SARS-CoV-2 emerged from animals and is now easily transmitted between people. Sporadic detection of natural cases in animals alongside successful experimental infections of pets, such as cats, ...ferrets and dogs, raises questions about the susceptibility of animals under natural conditions of pet ownership. Here, we report a large-scale study to assess SARS-CoV-2 infection in 919 companion animals living in northern Italy, sampled at a time of frequent human infection. No animals tested PCR positive. However, 3.3% of dogs and 5.8% of cats had measurable SARS-CoV-2 neutralizing antibody titers, with dogs from COVID-19 positive households being significantly more likely to test positive than those from COVID-19 negative households. Understanding risk factors associated with this and their potential to infect other species requires urgent investigation.
A
bstract
We present a detailed study of the production of dark matter in the form of a sterile neutrino via freeze-in from decays of heavy right-handed neutrinos. Our treatment accounts for thermal ...effects in the effective couplings, generated via neutrino mixing, of the new heavy neutrinos with the Standard Model gauge and Higgs bosons and can be applied to several low-energy fermion seesaw scenarios featuring heavy neutrinos in thermal equilibrium with the primordial plasma. We find that the production of dark matter is not as suppressed as to what is found when considering only Standard Model gauge interactions. Our study shows that the freeze-in dark matter production could be efficient.
Feline coronavirus (FCoV) exists as two different genotypes, FCoV type I and II, each including two biotypes, feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV), the ...latter being a virulent variant originating from the former virus. Recently, two amino acid substitutions, M1058L and S1060A, within the spike protein have been associated to the FECV/FIPV virulence change. In this study, we have analysed the frequency of detection of such mutations in FIPV and FECV strains circulating in Italian cats and obtained information about their evolutionary relationships with reference isolates. A total of 40 FCoV strains, including 19 strains from effusions or tissue samples of FIP cats and 21 strains from faecal samples of non-FIP cats, were analysed. Mutation M1058L was detected in 16/18 FCoV-I and 1/1 FCoV-II strains associated with FIP, while change S1060A was presented by two FIPV strains. By phylogenetic analysis, FCoV sequences clustered according to the genotype but not according to the biotype, with FECV/FIPV strains recovered from the same animal being closely related. Further studies are needed to better define the genetic signatures associated with the FECV/FIPV virulence shift.
•Spike protein sequences from FCoV strains circulating in Italian FIP (n = 19) and non-FIP (n = 21) cats were analysed.•Mutations M1058L and S1060A were detected in 17 and 2 FCoV strains associated with FIP, respectively.•By phylogenetic analysis, FCoV sequences clustered according to the genotype but not according to the pathotype.•FECV/FIPV strains recovered from the same animal were closely related at the phylogenetic level.•Further studies will better define the genetic signatures associated with the FCoV virulence shift.
Abstract Both egg- and cell-adapted canine distemper virus (CDV) vaccines are suspected to retain residual virulence, especially if administered to immuno-suppressed animals, very young pups or to ...highly susceptible animal species. In the early 1980s, post-vaccine encephalitis was reported in dogs from various parts of Britain after administration of a particular batch of combined CDV Rockborn strain/canine adenovirus type-1 vaccine, although incrimination of the Rockborn strain was subsequently retracted. Notwithstanding, this, and other reports, led to the view that the Rockborn strain is less attenuated and less safe than other CDV vaccines, and the Rockborn strain was officially withdrawn from the markets in the mid 1990s. By sequencing the H gene of the strain Rockborn from the 46th laboratory passage, and a commercial vaccine (Candur® SH+P, Hoechst Rousell Vet GmbH), the virus was found to differ from the commonly used vaccine strain, Onderstepoort (93.0% nt and 91.7% aa), and to resemble more closely (99.6% nt and 99.3% aa) a CDV strain detected in China from a Lesser Panda ( Ailurus fulgens ). An additional four CDV strains matching (>99% nt identity) the Rockborn virus were identified in the sequence databases. Also, Rockborn-like strains were identified in two vaccines currently in the market. These findings indicate that Rockborn-like viruses may be recovered from dogs or other carnivores with distemper, suggesting cases of residual virulence of vaccines, or circulation of vaccine-derived Rockborn-like viruses in the field.
In this work we consider a simple extension of the Standard Model involving additional fermionic singlets and assume an underlying inverse seesaw mechanism (with one or more right-handed neutrinos ...and one or more sterile fermions) for neutrino mass generation. Under the assumption that both sterile states and right-handed neutrinos are present, our goal is to determine which is the minimal inverse seesaw realisation that accounts for neutrino data while at the same time complying with all experimental requirements (electroweak precision tests and laboratory constraints). This study aims at identifying the minimal inverse seesaw realisation for the 3-flavour and for the 3 + more-mixing schemes, the latter giving an explanation for the reactor anomalies and/or providing a possible candidate for the dark matter of the Universe. Based on a perturbative approach, our generic study shows that in the class of inverse seesaw models giving rise to a 3-flavour flavour mixing scheme, only two mass scales are relevant (the light neutrino mass scale, mν and the mass of the right-handed neutrinos, MR) while in the case of a 3+1-mixing scheme, an additional mass scale (μ∈mν,MR) is required. For each of the two obtained inverse seesaw frameworks, we conduct a thorough numerical analysis, providing predictions for the hierarchy of the light neutrino spectrum and for the effective mass in neutrinoless double beta decay.
Canine distemper virus (CDV) is a highly contagious viral pathogen causing lethal disease in dogs and other mammalians. A high degree of genetic variation is found between recent CDV strains and the ...old CDV isolates used in the vaccines and such genetic variation is regarded as a possible cause of the increasing number of CDV-related diseases in dogs. The H gene shows the greatest extent of genetic variation that allows for distinction of various lineages, according to a geographical pattern of distribution and irrespective of the species of identification. In the present study, hemagglutinin (H) genes obtained from field strains detected from clinical specimens of Italian dogs were analyzed genetically. Phylogenetic analysis revealed that a homogeneous group of CDV strains is widespread in Italian dogs, all which are included into the European lineage. Unexpectedly, strains 179/04 and 48/05 clustered along with CDVs of the Arctic lineage, the highest identity being to strain GR88 (98.0 and 98.4%
aa, respectively). The full-length sequence of a red fox CDV strain, 207/00 was also determined and analyzed. The H protein of the fox CDV strain was unrelated to strains within the major European lineage. These results suggest that at least three different CDV lineages are present in Italy.
Thirty-nine parvovirus strains contained in faecal samples collected in Italy (n=34) and UK (n=5) from cats with feline panleukopenia were characterized at the molecular level. All viruses were ...proven to be true feline panleukopenia virus (FPLV) strains by a minor groove binder probe assay, which is able to discriminate between FPLV and the closely related canine parvovirus type 2. By using sequence analysis of the VP2 gene, it was found that the FPLV strains detected in Italy and UK were highly related to each other, with a nucleotide identity of 99.1-100 and 99.4-99.8 % among Italian and British strains, respectively, whereas the similarities between all the sequences analysed were 98.6-100 %. Eighty-eight variable positions were detected in the VP2 gene of the field and reference FPLV strains, most of which were singletons. Synonymous substitutions (n=57) predominated over non-synonymous substitutions (n=31), and the ratio between synonymous and non-synonymous substitutions (dN/dS) was 0.10, thus confirming that evolution of FPLV is driven by random genetic drift rather than by positive selection pressure. Some amino acid mutations in the VP2 protein affected sites that are thought to be responsible for antigenic and biological properties of the virus, but no clear patterns of segregation and genetic markers, were identified, confirming that FPLV is in evolutionary stasis.
The VP4 gene of a G5 Italian porcine rotavirus strain, 344/04-1, was nontypeable by PCR genotyping. The amino acid sequence of the full-length VP4 protein had low identity (<=76.6%) with the ...homologous sequences of representative strains of the remaining P genotypes, providing evidence for a novel P genotype.
•Bubaline herpesvirus 1 (BuHV-1) was detected in trigeminal ganglia of experimentally infected goats.•Latent infection was reactivated with desamethasone in a goat 10 months after the experimental ...infection.•The reactivated virus was shed through nasal and ocular secretions.•Reactivation of BuHV-1 in goat resulted in a 4-fold increase in the antibody titer.
Latent infection is a common mechanism used by several alphaherpesviruses to persist in their host but it is not clear whether this mechanism is also triggered in heterologous infections. Cross-species infections have been documented repeatedly for alphaherpesviruses of ruminants, a group of closely related viruses. Herewith we report latent infection with bubaline alphaherpesvirus 1 (BuHV-1) in experimentally infected goats and subsequent virus reactivation after treatment with dexamethasone (DMS) at 10 months after infection. After DMS treatment, the virus was isolated in one such animal in the nasal swabs from day 3 to 9 post treatment and in the ocular swabs at day 6. The goat was euthanized 48 days after DMS treatment and viral DNA was detected by PCR in the trigeminal ganglia and in two cervical ganglia. Additionally, BuHV-1 DNA was detected by PCR in the trigeminal ganglia of the other 3 goats.
Summary
The clinical features and economic impact of the infection caused by an emerging group of pestiviruses, namely HoBi‐like pestivirus, in a cattle herd of southern Italy are reported. In 2011, ...the virus was first associated with respiratory disease, causing an abortion storm after 1 year and apparently disappearing for the following 3 years after persistently infected calves were slaughtered. However, in 2014, reproductive failures and acute gastroenteritis were observed in the same herd, leading to a marked decrease of productivity. A HoBi‐like strain closely related to that responsible for previous outbreaks was detected in several animals. Application of an intensive eradication programme, based on the detection and slaughtering of HoBi‐like pestivirus persistently infected animals, resulted in a marked improvement of the productive performances.
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