A scale down version of a multi component production plant, including a cell breeding reactor, a protein production reactor and a disc clarifier for enzyme separation is combined to an Integrated ...Bioprocess. Fully automated and global observable multistage parallel process courses are developed using industrial process control systems and atline measurements for enzyme concentration and enzyme activity. Optimal production conditions were found by application of DoE - Design of Experiment.
Two derivates of Escherichia coli BL21 (DE3) were used for the expression of an IPTG-inducible fluorescent fusion protein ZsGreen-hEphB2-6His. The influence of cell specific growth rate, liquid phase ...temperature and IPTG inducer concentration on soluble and insoluble protein expression was studied. A multi-fermenter system BIOSTAT® Qplus-6 was configured for the execution of automated screening and optimisation experiments according to a Design of Experiments (DoE) strategy.
An extensive monitoring system is build up to fully automate and scan production processes of recombinant Escherichia coli for optimal process performance. It is based on in-line, on-line and at-line ...measurements for substrates and products. MATLABr is connected via OPC OPC Object linked and embedded (OLE) for process control to the SCADA
SCADA Supervisory control and data acquisition system MFCS/winr to realise on-line calculations of cell specific and volumetric reaction rates, based on the applied analytical systems. For on-line determination of intracellular target protein recombinant GFP is expressed either as single or fusion protein.
BACKGROUND: The technique of laparoscopic cholecystectomy (LC) has evolved since its adoption in the late 1980s. We sought to document these changes and assess whether patient outcomes were ...influenced during this maturational process. STUDY DESIGN: A prospective data base was used to record the outcomes of all LCs performed in an academic surgeon's practice. Trends over time among 1,165 consecutive patients were assessed by comparing the first 100 LCs (group I), the middle 100 LCs (group II), and the most recent 100 LCs (group III). RESULTS: During a 93-month period with 1,165 patients undergoing LC, 25 procedures (2.1%) were converted to open cholecystectomy. Perioperative complications occurred in 31 patients (3%): grade I in 9 (0.8%), grade II in 16 (1.4%), grade III in 5 (0.4%), and grade IV (death) in 1 (0.1%). Length of hospital stay and convalescence were 1.1 +/- 0.1 and 9.5 +/- 0.5 days, respectively. Nineteen patients (2%) were readmitted early after operation and 10 (1%) developed long-term complications (port-site hernia or retained stone). In group III, cholangiography was largely replaced by intraoperative ultrasonography for ductal evaluation. Operating room time decreased, while the rates of conversion, morbidity, and readmission remained the same. Patients had higher ASA classifications in the latter two groups, whereas operative charges were greater in Group III than in Groups I and II. These trends occurred even though most procedures are currently performed by residents, and fewer LCs are being done. CONCLUSIONS: Laparoscopic cholecystectomy has matured into a more efficient operation, yet remains safe with low morbidity when performed by residents at an academic institution.
Integrated Bioprocesses as a direct technological link between methods for the cultivation of the yeast Pichia pastoris and additional down-stream procedures ranging from cross-flow filtration for ...product recovery up to purification with combined chromatography systems are developed for a fully automated production of recombinant pharmaceutical proteins. The set-up of an appropriate 40 l pilot plant with an extended Process Analytical Technology (PAT) for on-line monitoring of important process variables, e.g. the recombinant product, as well as the development of complex control strategies by means of Apparent Processing with a real-time simulation system are explained.
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