An extensive monitoring system of high cell density cultivations (HCDC) with Escherichia coli was developed. It is based on at-line measurements of the main carbon source glucose, the nitrogen source ...ammonia and the undesired by-product acetate using flow analysis techniques. Besides, MATLAB® was connected to the SCADA system MFCS/win via OPC. Experiments were carried out with two different strains, wild type strain E. coli K12 TG1 (DSMZ 6056) and E. coli JM 105 with green fluorescence protein (GFP). Final cell densities up to 140 gl-1 were reached
During the past years one amplified research topic of the University of Applied Sciences in Hamburg was the purification of high nitrogen and methanol loaded waste water with simultaneous ...nitrification and denitrification processes. These include modern methods of bioprocess and automation technology as well as analytical techniques and simulation tools. The apparent processing was used to establish an advanced controlled waste water treatment process.
An integrated bioprocess is built up for production of an antagonist of recombinant monocyte chemoattractant protein-1 with Pichia pastoris.
The process line based on full automated cultivation ...procedures includes micro-and ultra-filtration membrane modules for in-situ interactions as well as liquid chromatography methods for post cultivation opcrations.
Summary
Background
Prenatal exposure to traffic pollution has been associated with faster infant weight gain, but implications for cardiometabolic health in later childhood are unknown.
Methods
Among ...1418 children in Project Viva, a Boston‐area pre‐birth cohort, we assessed anthropometric and biochemical parameters of cardiometabolic health in early (median age 3.3 years) and mid‐ (median age 7.7 years) childhood. We used spatiotemporal models to estimate prenatal and early life residential PM2.5 and black carbon exposure as well as traffic density and roadway proximity. We performed linear regression analyses adjusted for sociodemographics.
Results
Children whose mothers lived close to a major roadway at the time of delivery had higher markers of adverse cardiometabolic risk in early and mid‐childhood. For example, total fat mass was 2.1 kg (95%CI: 0.8, 3.5) higher in mid‐childhood for children of mothers who lived <50 m vs. ≥200 m from a major roadway. Black carbon exposure and traffic density were generally not associated with cardiometabolic parameters, and PM2.5 exposure during the year prior was paradoxically associated with improved cardiometabolic profile.
Conclusions
Infants whose mothers lived close to a major roadway at the time of delivery may be at later risk for adverse cardiometabolic health.
A powerful tool for biotechnological research is a mystat, a bioreactor system which allows to control the cell specific growth rate. A typical application of the mystat is a high cell density ...cultivation (HCDC) of E. coli up to 100 g/l dry weight without any change in cell regulation. This paper describes the biotechnological background of the control method, the development of a proper decentralized automation structure in CAN-technology, the testing of the control system by means of online-simulation methods and an experimental verification.
Progressive multifocal leukoencephalopathy (PML) is an infectious disease of the central nervous system caused by the JC virus. Progressive multifocal leukoencephalopathy represents a reactivation of ...the JC virus after long-standing immunosuppression. Also, PML plays an important role as an opportunistic infection in patients with AIDS. The average time of survival in patients with PML in combination with chronic lymphatic leukemia (CLL) (n = 17 in the literature) is 4.3 months, and therapeutic options are not established. We report the case of a patient with CLL and PML. Clinical symptoms are slight hemiparesis of the right side, mainly appearing as a disturbance of motor function. In MRI, a typical subcortical lesion was shown, and JC virus DNA was positive in the CSF by PCR. Because of first positive results in treatment of PML in patients with AIDS, therapy with cidofovir was started. After treatment for 16 months, symptoms are stable, the PML-induced lesions in MRI are in regression, and JC virus DNA is not detectable in the CSF.
Objectives This study sought to evaluate the association of air pollution with the onset of atrial fibrillation (AF). Background Air pollution in general and more specifically particulate matter has ...been associated with cardiovascular events. Although ventricular arrhythmias are traditionally thought to convey the increased cardiovascular risk, AF may also contribute. Methods Patients with dual chamber implantable cardioverter-defibrillators (ICDs) were enrolled and followed prospectively. The association of AF onset with air quality including ambient particulate matter <2.5 μm aerodynamic diameter (PM2.5 ), black carbon, sulfate, particle number, NO2 , SO2 , and O3 in the 24 h prior to the arrhythmia was examined utilizing a case-crossover analysis. In sensitivity analyses, associations with air pollution between 2 and 48 h prior to the AF were examined. Results Of 176 patients followed for an average of 1.9 years, 49 patients had 328 episodes of AF lasting ≥30 s. Positive but nonsignificant associations were found for PM2.5 in the prior 24 h, but stronger associations were found with shorter exposure windows. The odds of AF increased by 26% (95% confidence interval: 8% to 47%) for each 6.0 μg/m3 increase in PM2.5 in the 2 h prior to the event (p = 0.004). The odds of AF were highest at the upper quartile of mean PM2.5. Conclusions PM was associated with increased odds of AF onset within hours following exposure in patients with known cardiac disease. Air pollution is an acute trigger of AF, likely contributing to the pollution-associated adverse cardiac outcomes observed in epidemiological studies.
The methylotrophic yeast Pichia pastoris is used for expression of pharmaceutical proteins like 1-8del MCP-1 and vMIP-II. Here the proteins are secreted and these extra cellular products open new ...strategies in production processes.
The combining of complex cultivations and primary down stream steps with cross flow filtration units leads to integrated bioprocessing. The development of optimised sequential production strategies in research and industrial scale needs on-line measurement of substrates and products up to the recombinant protein itself.
Full automated processes are designed to analyse integrated filtration steps for modeling of cross flow units and combined bioreaction. The transfer of research work into industrial pilot plant scale is also demonstrated.
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