Comparisons of the material qualities of pressed, milled, and 3D-printed occlusal devices are sparse, complicating informed decisions on material choice.
The purpose of this in vitro study was to ...compare the material properties of pressed, milled, and 3D-printed resins, as well as how these are affected by thermal aging. These data were then used to estimate the likely clinical performance of the tested materials.
Three pressed (ProBase Cold; Ivoclar Vivadent AG, Palapress clear; Kulzer GmbH, Aesthetic Blue clear; Candulor), 3 milled (Temp Premium Flexible Transpa; Zirkonzahn, idodentine PMMA transparent; Unión Dental S.A., Yamahachi PMMA clear; Yamahachi Dental MFG), and three 3D-printed (Freeprint splint; DETAX GmbH, LuxaPrint Ortho Plus; DMG GmbH, Nextdent Ortho Clear; Vertex-Dental B.V.) resin materials were evaluated. Flexural strength, Martens hardness (HM), Vickers hardness (HV), water sorption, water solubility, and surface topography were analyzed. The tests were carried out after 50 hours of water storage at 37 °C (baseline) and after simulated aging (50 hours of water storage at 37 °C, followed by 20 000 thermocycles TC at 5 °C and 55 °C).
At baseline, the mean flexural strength values were 92.8 to 99.5 MPa for pressed, 95.1 to 122.0 MPa for milled, and 19.5 to 91.3 MPa for 3D-printed materials. After aging, these values were 87.6 to 93.5 MPa for pressed, 93.1 to 116.0 MPa for milled, and 13.0 to 63.3 MPa for 3D-printed resins. The mean HM values were 130.1 to 134.1 N/mm for pressed and 130.3 to 158.5 N/mm for milled resins. After aging, the mean HM ranged from 121.6 to 124.2 N/mm for pressed and 116.2 to 149.7 N/mm for milled resins. The mean HV values were 18.2 to 19.9 for pressed and 18.4 to 23.0 for milled resins before aging and 16.9 to 18.7 for pressed and 17.3 to 22.3 N/mm for milled resins after aging. Printed resins could not be measured. At baseline, the mean modulus of elasticity ranged from 4.6 to 4.8 GPa for pressed and from 4.7 to 5.3 GPa for milled resins. For 3D-printed resins, only 1 material could be measured (3.7 GPa). The mean sorption values were 8.6 to 9.2 μg/mm3 for pressed, 7.9 to 10.5 μg/mm3 for milled, and 9.2 to 21.2 μg/mm3 for additive resins. After aging, these values were 21.1 to 22.6 μg/mm3 for pressed, 20.5 to 23.7 μg/mm3 for milled, and 19.4 to 45.5 μg/mm3 for 3D-printed resins. The mean solubility values ranged from 0.3 to 1.4 μg/mm3 for pressed, 0.4 to 1.7 μg/mm3 for milled, and −3.5 to 11 μg/mm3 for 3D-printed materials.
Pressed and milled resins can be considered equivalent in terms of their material properties. Relative to the pressed and milled resins, the 3D-printed resins had lower flexural strength and hardness values and higher water sorption and solubility.
Modern communication systems rely on efficient quadrature amplitude modulation formats that encode information on both the amplitude and phase of an electromagnetic carrier. Coherent detection of ...such signals typically requires complex receivers that contain a continuous-wave local oscillator as a phase reference and a mixer circuit for spectral down-conversion. In optical communications, the so-called Kramers–Kronig scheme has been demonstrated to simplify the receiver, reducing the hardware to a single photodiode1–3. In this approach, a local-oscillator tone is transmitted along with the signal, and the amplitude and phase of the complex signal envelope are digitally reconstructed from the photocurrent by exploiting their Kramers–Kronig-type relation4–6. Here, we transfer the Kramers–Kronig scheme to high-speed wireless communications at terahertz carrier frequencies. To this end, we generalize the approach to account for non-quadratic receiver characteristics and employ a Schottky-barrier diode as a nonlinear receiver element. Using 16-state quadrature amplitude modulation, we transmit a net data rate of 115 Gbit s−1 at a carrier frequency of 0.3 THz over a distance of 110 m.The Kramers–Kronig approach is applied to high-capacity, free-space terahertz communications, bringing a greatly simplified receiver design.
Ki-67 gene expression Uxa, Sigrid; Castillo-Binder, Paola; Kohler, Robin ...
Cell death and differentiation,
12/2021, Letnik:
28, Številka:
12
Journal Article
Recenzirano
Odprti dostop
Ki-67 serves as a prominent cancer marker. We describe how expression of the MKI67 gene coding for Ki-67 is controlled during the cell cycle. MKI67 mRNA and Ki-67 protein are maximally expressed in G
...phase and mitosis. Expression is dependent on two CHR elements and one CDE site in the MKI67 promoter. DREAM transcriptional repressor complexes bind to both CHR sites and downregulate the expression in G
/G
cells. Upregulation of MKI67 transcription coincides with binding of B-MYB-MuvB and FOXM1-MuvB complexes from S phase into G
/M. Importantly, binding of B-MYB to the two CHR elements correlates with loss of CHR-dependent MKI67 promoter activation in B-MYB-knockdown experiments. In knockout cell models, we find that DREAM/MuvB-dependent transcriptional control cooperates with the RB Retinoblastoma tumor suppressor. Furthermore, the p53 tumor suppressor indirectly downregulates transcription of the MKI67 gene. This repression by p53 requires p21/CDKN1A. These results are consistent with a model in which DREAM, B-MYB-MuvB, and FOXM1-MuvB together with RB cooperate in cell cycle-dependent transcription and in transcriptional repression following p53 activation. In conclusion, we present mechanisms how MKI67 gene expression followed by Ki-67 protein synthesis is controlled during the cell cycle and upon induction of DNA damage, as well as upon p53 activation.
Consumption Taxes and Corporate Investment Jacob, Martin; Michaely, Roni; Müller, Maximilian A.
The Review of financial studies,
08/2019, Letnik:
32, Številka:
8
Journal Article
Recenzirano
Consumers nominally pay the consumption tax, but theoretical and empirical evidence is mixed on whether corporations partly shoulder this burden, thereby affecting corporate investment. Using a ...quasi-natural experiment, we show that consumption taxes decrease investment. Firms facing more elastic demand decrease investment more strongly, because they bear more of the consumption tax. We corroborate the validity of our findings using 86 consumption tax changes in a cross-country panel. We document two mechanisms underlying the investment response: reduced firms’ profitability and lower aggregate consumption. Importantly, the magnitude of the investment response to consumption taxes is similar to that of corporate taxes.
•Serological studies on SARS- and MERS-coronavirus (CoV) diagnostics were reviewed.•Different types of serological assays and variable antigens were compared.•Immunogenic epitopes of CoV spike ...proteins were less conserved than nucleocapsid proteins.•Use of spike proteins was found to be superior over nucleocapsid proteins.•Applicability of serological assays for analysis of animal sera was reviewed.
More than a decade after the emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002/2003 the occurrence of a novel CoV termed Middle East respiratory syndrome (MERS) CoV challenges researchers and public health authorities. To control spread and finally contain novel viruses, rapid identification and subsequent isolation of infected individuals and their contacts is of utmost importance. Next to methods for nucleic acid detection, validated serological assays are particularly important as the timeframe for antibody detection is less restricted. During the SARS-CoV epidemic a wide variety of serological diagnostic assays were established using multiple methods as well as different viral antigens. Even though the majority of the developed assays showed high sensitivity and specificity, numerous studies reported on cross-reactive antibodies to antigens from wide-spread common cold associated CoVs. In order to improve preparedness and responsiveness during future outbreaks of novel CoVs, information and problems regarding serological diagnosis that occurred during the SARS-CoV should be acknowledged.
In this review we summarize the performance of different serological assays as well as the applicability of the two main applied antigens (spike and nucleocapsid protein) used during the SARS-CoV outbreak. We highlight challenges and potential pitfalls that occur when dealing with a novel emerging coronavirus like MERS-CoV. In addition we describe problems that might occur when animal sera are tested in serological assays for the identification of putative reservoirs. Finally, we give a recommendation for a serological testing scheme and outline necessary improvements that should be implemented for a better preparedness.
Nature developed numerous solutions to solve various technical problems related to material surfaces by combining the physico-chemical properties of a material with periodically aligned ...micro/nanostructures in a sophisticated manner. The utilization of ultra-short pulsed lasers allows mimicking numerous of these features by generating laser-induced periodic surface structures (LIPSS). In this review paper, we describe the physical background of LIPSS generation as well as the physical principles of surface related phenomena like wettability, reflectivity, and friction. Then we introduce several biological examples including e.g., lotus leafs, springtails, dessert beetles, moth eyes, butterfly wings, weevils, sharks, pangolins, and snakes to illustrate how nature solves technical problems, and we give a comprehensive overview of recent achievements related to the utilization of LIPSS to generate superhydrophobic, anti-reflective, colored, and drag resistant surfaces. Finally, we conclude with some future developments and perspectives related to forthcoming applications of LIPSS-based surfaces.
Context. Starbursts are galaxies undergoing massive episodes of star formation. The combined effect of stellar winds from hot stars and supernova explosions creates a high-temperature cavity in the ...nuclear region of these objects. The very hot gas expands adiabatically and escapes from the galaxy creating a superwind which sweeps matter from the galactic disk. The superwind region in the halo is filled with a multi-phase gas with hot, warm, cool, and relativistic components. Aims. The shocks associated with the superwind of starbursts and the turbulent gas region of the bubble inflated by them might accelerate cosmic rays up to high energies. In this work we calculate the cosmic ray production associated with the superwind using parameters that correspond to the nearby southern starburst galaxy NGC 253, which has been suggested as a potential accelerator of ultra-high-energy cosmic rays. Methods. We evaluate the efficiency of both diffusive shock acceleration (DSA) and stochastic diffusive acceleration (SDA) in the superwind of NGC 253. We estimate the distribution of both hadrons and leptons and calculate the corresponding spectral energy distributions of photons. The electromagnetic radiation can help to discriminate between the different scenarios analyzed. Results. We find that the strong mass load of the superwind, recently determined through ALMA observations, strongly attenuates the efficiency of DSA in NGC 253, whereas SDA is constrained by the age of the starburst. Conclusions. We conclude that NGC 253 and similar starbursts can only accelerate iron nuclei beyond ~1018 eV under very special conditions. If the central region of the galaxy harbors a starved supermassive black hole of ~106 M⊙, as suggested by some recent observations, a contribution in the range 1018−1019 eV can be present for accretion rates ṁ ~ 10−3 in Eddington units. Shock energies of the order of 100 EeV might only be possible if very strong magnetic field amplification occurs close to the superwind.
Coronavirus disease 2019 (COVID-19) is an acute infection of the respiratory tract that emerged in late 2019
. Initial outbreaks in China involved 13.8% of cases with severe courses, and 6.1% of ...cases with critical courses
. This severe presentation may result from the virus using a virus receptor that is expressed predominantly in the lung
; the same receptor tropism is thought to have determined the pathogenicity-but also aided in the control-of severe acute respiratory syndrome (SARS) in 2003
. However, there are reports of cases of COVID-19 in which the patient shows mild upper respiratory tract symptoms, which suggests the potential for pre- or oligosymptomatic transmission
. There is an urgent need for information on virus replication, immunity and infectivity in specific sites of the body. Here we report a detailed virological analysis of nine cases of COVID-19 that provides proof of active virus replication in tissues of the upper respiratory tract. Pharyngeal virus shedding was very high during the first week of symptoms, with a peak at 7.11 × 10
RNA copies per throat swab on day 4. Infectious virus was readily isolated from samples derived from the throat or lung, but not from stool samples-in spite of high concentrations of virus RNA. Blood and urine samples never yielded virus. Active replication in the throat was confirmed by the presence of viral replicative RNA intermediates in the throat samples. We consistently detected sequence-distinct virus populations in throat and lung samples from one patient, proving independent replication. The shedding of viral RNA from sputum outlasted the end of symptoms. Seroconversion occurred after 7 days in 50% of patients (and by day 14 in all patients), but was not followed by a rapid decline in viral load. COVID-19 can present as a mild illness of the upper respiratory tract. The confirmation of active virus replication in the upper respiratory tract has implications for the containment of COVID-19.
The gut mucosal epithelium separates the host from the microbiota, but enteropathogens such as Salmonella Typhimurium (S.Tm) can invade and breach this barrier. Defenses against such acute insults ...remain incompletely understood. Using a murine model of Salmonella enterocolitis, we analyzed mechanisms limiting pathogen loads in the epithelium during early infection. Although the epithelium-invading S.Tm replicate initially, this intraepithelial replicative niche is restricted by expulsion of infected enterocytes into the lumen. This mechanism is compromised if inflammasome components (NAIP1-6, NLRC4, caspase-1/-11) are deleted, or ablated specifically in the epithelium, resulting in ∼100-fold higher intraepithelial loads and accelerated lymph node colonization. Interestingly, the cytokines downstream of inflammasome activation, interleukin (IL)-1α/β and IL-18, appear dispensable for epithelial restriction of early infection. These data establish the role of an epithelium-intrinsic inflammasome, which drives expulsion of infected cells to restrict the pathogen’s intraepithelial proliferation. This may represent a general defense mechanism against mucosal infections.
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•Salmonella initially invade and replicate within enterocytes in vivo•Expulsion of infected enterocytes restricts Salmonella intraepithelial replication•Intraepithelial bacterial restriction requires NAIPs and NLRC4, but not IL-1/18•Infected enterocyte expulsion depends on epithelium-intrinsic inflammasome activity
The gut is covered by a single layer of epithelial cells, which can become a portal for pathogen entry. Using a murine model of Salmonella enterocolitis, Sellin et al. show that activation of an epithelium-intrinsic inflammasome early after bacterial invasion drives expulsion of infected cells to restrict intraepithelial pathogen proliferation.
From all non-human animals dogs are very likely the best decoders of human behavior. In addition to a high sensitivity to human attentive status and to ostensive cues, they are able to distinguish ...between individual human faces and even between human facial expressions. However, so far little is known about how they process human faces and to what extent this is influenced by experience. Here we present an eye-tracking study with dogs emanating from two different living environments and varying experience with humans: pet and lab dogs. The dogs were shown pictures of familiar and unfamiliar human faces expressing four different emotions. The results, extracted from several different eye-tracking measurements, revealed pronounced differences in the face processing of pet and lab dogs, thus indicating an influence of the amount of exposure to humans. In addition, there was some evidence for the influences of both, the familiarity and the emotional expression of the face, and strong evidence for a left gaze bias. These findings, together with recent evidence for the dog's ability to discriminate human facial expressions, indicate that dogs are sensitive to some emotions expressed in human faces.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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