Magnetic porous silicon flakes (MPSF) were obtained from mesoporous silicon layers formed by multi-step anodization and subsequent composite formation with Fe oxide nanoparticles by thermal ...annealing. The magnetic nanoparticles adhered to the surface and penetrated inside the pores. Their structure evolved as a result of the annealing treatments derived from X-ray diffraction and X-ray absorption analyses. Moreover, by tailoring the magnetic load, the dynamic and hydrodynamic properties of the particles were controlled, as observed by the pressure displayed against a sensor probe. Preliminary functionality experiments were performed using an eye model, seeking potential use of MPSF as reinforcement for restored detached retina. It was observed that optimal flake immobilization is obtained when the MPSF reach values of magnetic saturation >10−4Am2g−1. Furthermore, the MPSF were demonstrated to be preliminarily biocompatible in vitro. Moreover, New Zealand rabbit in vivo models demonstrated their short-term histocompatibility and their magnetic functionality as retina pressure actuators.
The genus Trichoderma contains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, ...comprehensive overview of the genomic content of these species for "hot topic" research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism in T. reesei, T. atroviride, and T. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of each Trichoderma species discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved in N-linked glycosylation was detected, as were indications for the ability of Trichoderma spp. to generate hybrid galactose-containing N-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique to Trichoderma, and these warrant further investigation. We found interesting expansions in the Trichoderma genus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique to T. atroviride is the duplication of the alternative sulfur amino acid synthesis pathway.
Actinobacillus pleuropneumoniae is the causal agent of porcine pleuropneumonia, which is a highly contagious respiratory disease that affects swine nearly exclusively. An isolate with characteristics ...of some Pasteurellaceae family members (Gram-negative bacterium, pleomorphic, and NAD-dependent) was isolated from layer hens showing clinical signs of infectious coryza. This bacterium presented hemolysis on rabbit red blood cell agar plates, and PCR amplification and sequencing of its 16S rDNA gene indicated 99% identity with A. pleuropneumoniae serotypes 3 and 7. The presence of a putative apxIIA gene was also determined by PCR. A single, smooth colony of this bacterium inoculated in five, 7-day-old chicken embryos via the yolk sac route induced 100% mortality. However, inoculation into 10-wk-old, specific-pathogen-free chickens induced only light facial swelling, and reisolation of the inoculated bacterium was negative. Reporte de caso—Aislamiento de Actinobacillus pleuropneumoniae de gallinas de postura con signos clínicos de coriza infecciosa. Actinobacillus pleuropneumoniae es el agente causal de la pleuroneumonía porcina, una enfermedad respiratoria altamente contagiosa que ha sido considerada que afecta casi exclusivamente a los cerdos. Se obtuvo un aislamiento con características de algunos de los miembros de la familia Pasteurellaceae (bacteria Gram negativa, pleomórfica y dependiente de NAD) a partir de gallinas de postura que mostraban signos clínicos de coriza infecciosa. Esta bacteria produjo hemólisis en placas de agar sangre de conejo. La amplificación por PCR del gene rDNA 16S y su secuenciación indicó una identidad del 99% con el rDNA de A. pleuropneumoniae serotipos 3 y 7. La presencia de un supuesto gene apxIIA también determinada por PCR. Cuando se inocularon cinco huevos embrionados de pollo de siete días de edad con una colonia lisa de esta bacteria a través del saco vitelino se observó 100% de mortalidad. Sin embargo, su inoculación en aves libres de patógenos específicos de 10 semanas de edad indujo únicamente una inflamación facial ligera, y el re-aislamiento de la bacteria inoculada fue negativo.
The draft genome sequence of
strain CL serovar C is reported here. The genome comprises 154 contigs corresponding to 2.4 Mb with 41% G+C content and many insertion sequence (IS) elements, a ...characteristic not previously reported in
.
Actinobacillus pleuropneumoniaeis the causal agent of porcine pleuropneumonia, which is a highly contagious respiratory disease that affects swine nearly exclusively. An isolate with characteristics ...of somePasteurellaceaefamily members (Gram-negative bacterium, pleomorphic, and NAD-dependent) was isolated from layer hens showing clinical signs of infectious coryza. This bacterium presented hemolysis on rabbit red blood cell agar plates, and PCR amplification and sequencing of its 16S rDNA gene indicated 99% identity withA. pleuropneumoniaeserotypes 3 and 7. The presence of a putativeapxIIA gene was also determined by PCR. A single, smooth colony of this bacterium inoculated in five, 7-day-old chicken embryos via the yolk sac route induced 100% mortality. However, inoculation into 10-wk-old, specific-pathogen-free chickens induced only light facial swelling, and reisolation of the inoculated bacterium was negative.
Actinobacillus pleuropneumoniaees el agente causal de la pleuroneumonía porcina, una enfermedad respiratoria altamente contagiosa que ha sido considerada que afecta casi exclusivamente a los cerdos. Se obtuvo un aislamiento con características de algunos de los miembros de la familiaPasteurellaceae(bacteria Gram negativa, pleomórfica y dependiente de NAD) a partir de gallinas de postura que mostraban signos clínicos de coriza infecciosa. Esta bacteria produjo hemólisis en placas de agar sangre de conejo. La amplificación por PCR del gene rDNA 16S y su secuenciación indicó una identidad del 99% con el rDNA deA. pleuropneumoniaeserotipos 3 y 7. La presencia de un supuesto geneapxIIA también determinada por PCR. Cuando se inocularon cinco huevos embrionados de pollo de siete días de edad con una colonia lisa de esta bacteria a través del saco vitelino se observó 100% de mortalidad. Sin embargo, su inoculación en aves libres de patógenos específicos de 10 semanas de edad indujo únicamente una inflamación facial ligera, y el re-aislamiento de la bacteria inoculada fue negativo.
PDF
