Four new solvent-induced Co(II) complexes with chemical formulae {CoL(μ2-OAc)(MeOH)}2Co (1), {CoL(μ2-OAc)(EtOH)}2Co (2), {CoL(μ2-OAc)(Py)}2Co (3) and {CoL(μ2-OAc)(DMF)}2Co (4) (H2L = ...4-nitro-4′-chloro-2,2′-(1,3-propylene)dioxybis(nitrilomethylidyne)diphenol) have been synthesized and characterized by elemental analyses, FT–IR, UV–Vis spectra and single-crystal X-ray diffraction. Each of the prepared complexes, crystallizing in the space groups P–1 (1 and 4), P21/n (2) and P21/c (3), consists of three Co(II) atoms, two completely deprotonated (L)2− units, two μ2-acetato ligands and two coordinated solvent molecules. Although the four complexes 1–4 were synthesized in different solvents, it is worthwhile that the Co(II) atoms in the four complexes 1–4 adopt hexa–coordinated with slightly distorted octahedral coordination geometries, and the ratio of the ligand H2L to Co(II) atoms is 2:3. The complexes 2–4 possess a self-assembled infinite 1D, 2D and 1D supramolecular structures via the intermolecular hydrogen bonds, respectively. Magnetic measurement was performed in the complex 3.
Endometrial cancer stem cells (ECSCs) play a vital role in endometrial cancer (EC) metastasis, relapse, and chemoresistance. However, the molecular mechanisms that sustain ECSCs remain elusive. Here, ...we showed that the expression of UPF1 was upregulated in EC tissues and ECSCs and correlated with poor clinicopathological characteristics. UPF1 silencing suppressed ECSC hallmarks, such as sphere formation ability, carboplatin resistance, migration and invasion, and cell cycle progression. UPF1 regulated the behavior and fate of ECSCs by stabilizing LINC00963. LINC00963 further shares the same miRNA response element with the core transcription factor SOX2 and relieved the suppression of SOX2 by miR-508-5p in self-renewing ECSCs. Notably, inhibition of UPF1 and LINC00963 in combination severely impaired the in vivo tumorigenic potential of ECSCs. We demonstrate that the UPF1/LINC00963/miR-508-5p/SOX2 axis has potential value in modulating ECSC maintenance, chemoresistance, and tumorigenesis in EC, which highlights a novel promising target for EC treatment.
This study aimed to determine the cause of the outbreak of nosocomial adenoviral conjunctivitis in the Neonatal Intensive Care Unit (NICU) and the impact of infection control measures. The objectives ...of the present study included investigating the association between hospital-borne infection and adenoviral conjunctivitis, analyzing the possible risk factors, and setting bundled infection control measures, which were adjusted according to the control effect. This study also aimed to observe the effects of different intervention measures on controlling adenoviral conjunctivitis. During the first and second intervention periods, 635 and 597 patients in the NICU were enrolled, respectively. Ophthalmoscopy was performed in 188 ( first intervention) and 184 (second intervention) patients (P > 0.05) 417 and 457 times, respectively (P < 0.001). During the first intervention and second interventions, 13 patients and no patient had adenoviral conjunctivitis, respectively (P < 0.001). All adenoviral conjunctivitis cases were reported 6–27 days (mean, 12 days) after ophthalmoscopy. Hydrogen peroxide disinfection bundled measures can effectively restrict the prevalence of adenoviral conjunctivitis associated with ophthalmoscopy in premature infants.
A novel heterometallic platinum(
ii
)-silver(
i
) alkynyl complex PtAg(dppm)
2
(C&z.tbd;CC
6
H
4
COOCH
3
-4)
2
Cl (
1
) (dppm = bis(diphenylphosphino)methane) forms two different crystalline ...polymorphs that can be separately obtained on manipulating the recrystallization solvents. Two crystalline structures were confirmed by X-ray crystallography. Morphology-dependent photoluminescence behaviours were observed. Pale yellow rod-like crystals 1·CH
2
Cl
2
(
1a
) exhibit strongly cyan luminescence (
λ
em
= 476 nm,
τ
= 10.8 μs) at room temperature and have a rhombohedral
Pbca
structure, while weak green luminescent prismatic crystals 1·2CH
3
OH (
1b
) (
λ
em
= 491 nm,
τ
= 3.3 μs) have a triclinic
P
1&cmb.macr; structure with shorter Pt Ag distance (3.0418(3) Å), and significant intra/inter-molecular hydrogen bonds between O-H protons from different methanol molecules as H donors and an oxygen atom of the alkynyl ligand (
d
O-H O
= 2.185 Å) from the
1
unit, and the Cl atom from the neighbouring
1
unit (
d
O-H Cl
= 2.335 Å). Meanwhile,
1a
displays significant mechanochromism, with an intriguing emission color change from cyan to green under UV irradiation at 365 nm. The powder XRD test results indicate that the morphology transition between crystalline and partial amorphous states is responsible for the mechanochromism phenomenon of
1a
.
A novel complex PtAg(dppm)
2
(C&z.tbd;CC
6
H
4
COOCH
3
-4)
2
Cl with two crystal morphologies as rod-like and prism was separately obtained by manipulating the recrystallization solvents. They exhibit glamorous morphology-dependent photoluminescence.
Inspired by the discovery of adhesive proteins in mussels, we prepared a graphene-polydopamine (GPDA) hybrid, in which the commonly used graphene oxide was replaced by graphene synthesized through ...physical routes. Then, the hybrid was decorated with ultrafine Pd nanoparticles to obtain a catalyst that was stable and well-dispersed in polar solvents. The Pd nanoparticles on graphene-polydopamine (GPDAP) were 2.0 nm on average and showed good monodispersibility on the polydopamine-modified graphene, whereas the Pd particles on unmodified graphene (GP) were larger than 4.5 nm and were obviously aggregated. The catalytic activity of the catalyst was investigated in the reduction of 4-nitrophenol (4-NP), K 3 Fe(CN) 6 , methylene blue (MB) and rhodamine B (RhB), which are common industrial pollutants. A comparison between Pd/C (CP), GP and GPDA showed that the prepared catalyst, GPDAP, showed superior activity even when just a tiny amount of catalyst was added.
Photosynthesis provides food, fibre and fuel that support our society; understanding the mechanisms controlling dynamic changes in this process helps identify new options to improve photosynthesis. ...Photosynthesis shows diel changes, which have been largely attributed to external light/dark conditions, as well as internal gene expression and the post-translational modification of critical enzymes. Here we report diel fluctuations of magnesium (Mg) in rice (Oryza sativa) chloroplasts, which may function as a rhythm regulator contributing to the post-translational regulation of photosynthetic CO
assimilation in rice. We found that a chloroplast-localized Mg
transporter gene, OsMGT3, which is rhythmically expressed in leaf mesophyll cells, partly modulates Mg fluctuations in rice chloroplasts. Knockout of OsMGT3 substantially reduced Mg
uptake, as well as the amplitude of free Mg
fluctuations in chloroplasts, which was closely associated with a decrease in ribulose 1,5-bisphosphate carboxylase activity in vivo and a consequent decline in the photosynthetic rate. In addition, the mesophyll-specific overexpression of OsMGT3 remarkably improved photosynthetic efficiency and growth performance in rice. Taken together, these observations demonstrate that OsMGT3-dependent diel Mg fluctuations in chloroplasts may contribute to Mg-dependent enzyme activities for photosynthesis over the daily cycle. Enhancing Mg
input to chloroplasts could be a potential approach to improving photosynthetic efficiency in plants.
Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress.
Soybean NF-Y gene GmNFYA5 ...was identified to have the highest transcript level among all 21 NF-YA genes in soybean (Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter: GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes (ABI2, ABI3, NCED3, LEA3, RD29A, P5CS1, GmWRKY46, GmNCED2 and GmbZIP1) and ABA-independent genes (DREB1A, DREB2A, DREB2B, GmDREB1, GmDREB2 and GmDREB3) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo.
Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Acid soluble collagen (ASC) from scales of croceine croaker (ASC-C) was successfully isolated with the yield of 0.37% ± 0.08% (dry weight basis), and characterized as type I collagen on the basis of ...amino acid analysis and electrophoretic pattern. The antioxidant hydrolysate of ASC-C (ACH) was prepared through a two-stage in vitro digestion (4-h trypsin followed by 4-h pepsin), and three antioxidant peptides (ACH-P1, ACH-P2, and ACH-P3) were further isolated from ACH using ultrafiltration, gel chromatography, and RP-HPLC, and their amino acid sequences were identified as GFRGTIGLVG (ACH-P1), GPAGPAG (ACH-P2), and GFPSG (ACH-P3). ACH-P1, ACH-P2, and ACH-P3 showed good scavenging activities on hydroxyl radical (IC50 0.293, 0.240, and 0.107 mg/mL, respectively), DPPH radical (IC₅₀ 1.271, 0.675, and 0.283 mg/mL, respectively), superoxide radical (IC₅₀ 0.463, 0.099, and 0.151 mg/mL, respectively), and ABTS radical (IC₅₀ 0.421, 0.309, and 0.210 mg/mL, respectively). ACH-P3 was also effectively against lipid peroxidation in the model system. The antioxidant activities of three collagen peptides were due to the presence of hydrophobic amino acid residues within the peptide sequences. The collagen peptides might be used as antioxidant for the therapy of diseases associated with oxidative stress, or reducing oxidative changes during storage.
Prolonged hyperglycemia during diabetes mellitus (DM) is associated with severe complications that may affect both the anterior and posterior ocular segments, leading to impaired vision or blindness. ...The cornea is a vital part of the eye that has a dual role as a protective transparent barrier and as a major refractive structure and is likewise negatively affected by hyperglycemia in DM. Understanding the cellular and molecular mechanisms underlying the phenotypic changes associated with DM is critical to developing targeted therapies to promote tissue integrity. In this proof-of-concept study, we applied a cell sheet-based approach to generate stacked constructs of physiological corneal thickness using primary human corneal fibroblasts isolated from cadaveric control (healthy), Type 1 DM and Type 2 DM corneal tissues. Self-assembled corneal stromal sheets were generated after 2 weeks in culture, isolated, and subsequently assembled to create stacked constructs, which were evaluated using transmission electron microscopy. Analysis of gene expression patterns revealed significant downregulation of fibrotic markers, α-smooth muscle actin, and collagen type 3, with stacking in Type 2 DM constructs when compared to controls. IGF1 expression was significantly upregulated in Type 2 DM constructs compared to controls with a significant reduction induced by stacking. This study describes the development of a thicker, self-assembled corneal stromal construct as a platform to evaluate phenotypic differences associated with DM-derived corneal fibroblasts and enable the development of targeted therapeutics to promote corneal integrity.
The human genome encodes thousands of long noncoding RNA (lncRNA) genes; the function of majority of them is poorly understood. Aberrant expression of a significant number of lncRNAs is observed in ...various diseases, including cancer. To gain insights into the role of lncRNAs in breast cancer progression, we performed genome-wide transcriptome analyses in an isogenic, triple negative breast cancer (TNBC/basal-like) progression cell lines using a 3D cell culture model. We identified significantly altered expression of 1853 lncRNAs, including ~500 natural antisense transcript (NATs) lncRNAs. A significant number of breast cancer-deregulated NATs displayed co-regulated expression with oncogenic and tumor suppressor protein-coding genes in cis. Further studies on one such NAT, PDCD4-AS1 lncRNA reveal that it positively regulates the expression and activity of the tumor suppressor PDCD4 in mammary epithelial cells. Both PDCD4-AS1 and PDCD4 show reduced expression in TNBC cell lines and in patients, and depletion of PDCD4-AS1 compromised the cellular levels and activity of PDCD4. Further, tumorigenic properties of PDCD4-AS1-depleted TNBC cells were rescued by exogenous expression of PDCD4, implying that PDCD4-AS1 acts upstream of PDCD4. Mechanistically, PDCD4-AS1 stabilizes PDCD4 RNA by forming RNA duplex and controls the interaction between PDCD4 RNA and RNA decay promoting factors such as HuR. Our studies demonstrate crucial roles played by NAT lncRNAs in regulating post-transcriptional gene expression of key oncogenic or tumor suppressor genes, thereby contributing to TNBC progression.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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