MicroRNAs are small noncoding RNAs that play an important role in the regulation of various biological processes through their interaction with cellular messenger RNAs. They are frequently ...dysregulated in cancer and have shown great potential as tissue-based markers for cancer classification and prognostication. microRNAs are also present in extracellular human body fluids such as serum, plasma, saliva, and urine. Most of circulating microRNAs are present in human plasma and serum cofractionate with the Argonaute2 (Ago2) protein. However, circulating microRNAs have been also found in membrane-bound vesicles such as exosomes. Since microRNAs circulate in the bloodstream in a highly stable, extracellular form, they may be used as blood-based biomarkers for cancer and other diseases. A knowledge base of extracellular circulating miRNAs is a fundamental tool for biomedical research. In this work, we present miRandola, a comprehensive manually curated classification of extracellular circulating miRNAs. miRandola is connected to miRò, the miRNA knowledge base, allowing users to infer the potential biological functions of circulating miRNAs and their connections with phenotypes. The miRandola database contains 2132 entries, with 581 unique mature miRNAs and 21 types of samples. miRNAs are classified into four categories, based on their extracellular form: miRNA-Ago2 (173 entries), miRNA-exosome (856 entries), miRNA-HDL (20 entries) and miRNA-circulating (1083 entries). miRandola is available online at: http://atlas.dmi.unict.it/mirandola/index.html.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Vulnerability is a big issue for small inland urban centres, which are exposed to the risk of depopulation. In the climate of the centre-northern part of Italy, and in the context of the recent ...concentration of a high number of earthquakes in that area, seismic vulnerability can become the determinant cause of the final abandonment of a small town. In some Italian regions, as well as in Emilia Romagna, municipalities are implementing seismic vulnerability reduction policies based on the Emergency Limit Condition, which has become a basic point of reference for ordinary land planning. This study proposes an approach to seismic vulnerability reduction based on valuation planning for implementation within the general planning framework of the Faentina Union, a group of five small towns located in the southwestern part of the Province of Ravenna, Italy. This approach consists of three main stages: knowledge—the typological, constructive, and technological descriptions of the buildings, specifically concerning their degree of vulnerability; interpretation—analysis with the aim of outlining a range of hypotheses with respect to damage in case of a prospective earthquake; and planning—the identification of the courses of action intended to meaningfully reduce the vulnerability of buildings. This stage includes a cost modelling tool aimed at defining the trade-off between the extension and the intensity of the vulnerability reduction works, given the budget.
Among the fourteen towns most damaged by the earthquake that struck western Sicily in 1968, Poggioreale was subjected to total relocation a few kilometres away, and to the transfer of its whole ...population. More than half a century later, the damaged settlement is still largely recognisable and has been experiencing a new season of interest for some years now, both from the local community and various kinds of outsiders who imagine economic spin-offs of an uncertain nature in its enhancement. The research we present questions the chance of survival for the settlement, forgotten for decades, in the context of interventions aimed at its reuse. Alternative strategies are discussed: on the one hand, that of bringing the ancient centre back to life, even in part, with a limited inclusion of collective functions and without claiming to inhabit it again; on the other hand, and in the authors’ opinion, more likely, that of reusing the ruins as an archaeological area preserving their memory in a different form. In either case, the kinds of operations to be carried out on the body of the ancient buildings for their physical preservation play a key role. These issues are addressed as a whole through the canonical procedure of correlating the phases of knowledge, interpretation, and definition of minimal and compatible intervention criteria.
Among the fourteen towns most damaged by the earthquake that struck western Sicily in 1968, Poggioreale was subjected to total relocation a few kilometres away, and to the transfer of its whole ...population. More than half a century later, the damaged settlement is still largely recognisable and has been experiencing a new season of interest for some years now, both from the local community and various kinds of outsiders who imagine economic spin-offs of an uncertain nature in its enhancement. The research we present questions the chance of survival for the settlement, forgotten for decades, in the context of interventions aimed at its reuse. Alternative strategies are discussed: on the one hand, that of bringing the ancient centre back to life, even in part, with a limited inclusion of collective functions and without claiming to inhabit it again; on the other hand, and in the authors’ opinion, more likely, that of reusing the ruins as an archaeological area preserving their memory in a different form. In either case, the kinds of operations to be carried out on the body of the ancient buildings for their physical preservation play a key role. These issues are addressed as a whole through the canonical procedure of correlating the phases of knowledge, interpretation, and definition of minimal and compatible intervention criteria.
RNA Editing is a type of post-transcriptional modification that takes place in the eukaryotes. It alters the sequence of primary RNA transcripts by deleting, inserting or modifying residues. Several ...forms of RNA editing have been discovered including A-to-I, C-to-U, U-to-C and G-to-A. In recent years, the application of global approaches to the study of A-to-I editing, including high throughput sequencing, has led to important advances. However, in spite of enormous efforts, the real biological mechanism underlying this phenomenon remains unknown.
In this work, we present VIRGO (http://atlas.dmi.unict.it/virgo/), a web-based tool that maps Ato-G mismatches between genomic and EST sequences as candidate A-to-I editing sites. VIRGO is built on top of a knowledge-base integrating information of genes from UCSC, EST of NCBI, SNPs, DARNED, and Next Generations Sequencing data. The tool is equipped with a user-friendly interface allowing users to analyze genomic sequences in order to identify candidate A-to-I editing sites.
VIRGO is a powerful tool allowing a systematic identification of putative A-to-I editing sites in genomic sequences. The integration of NGS data allows the computation of p-values and adjusted p-values to measure the mapped editing sites confidence. The whole knowledge base is available for download and will be continuously updated as new NGS data becomes available.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
A-to-I RNA editing is an important mechanism that consists of the conversion of specific adenosines into inosines in RNA molecules. Its dysregulation has been associated to several human diseases ...including cancer. Recent work has demonstrated a role for A-to-I editing in microRNA (miRNA)-mediated gene expression regulation. In fact, edited forms of mature miRNAs can target sets of genes that differ from the targets of their unedited forms. The specific deamination of mRNAs can generate novel binding sites in addition to potentially altering existing ones.
This work presents miR-EdiTar, a database of predicted A-to-I edited miRNA binding sites. The database contains predicted miRNA binding sites that could be affected by A-to-I editing and sites that could become miRNA binding sites as a result of A-to-I editing.
miR-EdiTar is freely available online at http://microrna.osumc.edu/mireditar.
alessandro.lagana@osumc.edu or carlo.croce@osumc.edu
Supplementary data are available at Bioinformatics online.
Objective: We were aimed at evaluating the long-term impact of perinatal an omega-3 fatty acid-enriched diet on the mevalonate/cholesterol pathway in the brain of male offspring.
Methods: Female rats ...were fed with standard or omega-3 fatty acid-enriched diet during pregnancy and lactation. Liver, brain and plasma were collected from infant, adolescent and adult male offspring for subsequent biochemical and morphological analyses.
Results: The omega-3 enriched diet induced region-dependent changes of the 3-hydroxy 3-methylglutaryl Coenzyme A reductase in the brain and affected notably RhoA/CREB signaling and the nerve growth factor content in the hippocampus. Our data reveal a long-lasting impact of perinatal omega-3 fatty acid supplementation on hippocampal nerve growth factor levels mediated by reduced 3-hydroxy 3-methylglutaryl Coenzyme A reductase activation state and enhanced CREB signaling.
Discussion: These data underline the importance of the perinatal omega-3 enriched diet for adult brain function and reveal a new pathway important for nerve growth factor regulation.
Celotno besedilo
Dostopno za:
DOBA, FSPLJ, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
BACKGROUND: There is still no clear evidence available on the role of robot-assisted gait training (RAGT) in severe traumatic brain injury (TBI) and on the relationship between this intervention and ...cognitive impairment. OBJECTIVE: This study investigates the impact of the cognitive level at admission on functional recovery in a cohort of patients with severe TBI who received RAGT training within a multidisciplinary rehabilitation setting. METHODS: We included patients with gait disturbance due to a severe TBI. Patients were grouped into three classes according to their level of cognitive functioning (LCF) at admission (LCF 2-3; LCF 4-5-6; LCF 7-8). We collected demographics (sex, age), clinical data, and a set of outcome measures at admission and discharge. RESULTS: We registered 80 patients, 19 females and 61 males, 35.3 ± 14.85 years. Patients with a low cognitive level at admission were mostly subacute (p= 0.001). Cognitive impairment despite longer length stay in the hospital (LOS) (p= 0.001) did not preclude recovery after RAGT in terms of cognition (R2= 0.68; p< 0.0001), functional independence (R2= 0.30; p< 0.0001) and overall disability (R2= 0.32; p< 0.0001). CONCLUSION: Irrespective of their level of cognition, patients with severe TBI might benefit from RAGT during a multidisciplinary program.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, OILJ, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK, VSZLJ
•Glucocorticoids are an effective therapy for patients hospitalized with COVID-19.•The use of glucocorticoids was associated with a lower risk of in-hospital mortality.•A protective effect was mainly ...observed in patients with worse respiratory parameters.
Glucocorticoid therapy has emerged as an effective therapeutic option in hospitalized patients with coronavirus disease 2019 (COVID-19). This study aimed to focus on the impact of relevant clinical and laboratory factors on the protective effect of glucocorticoids on mortality.
A sub-analysis was performed of the multicenter Cardio-COVID-Italy registry, enrolling consecutive patients with COVID-19 admitted to 13 Italian cardiology units between 01 March 2020 and 09 April 2020. The primary endpoint was in-hospital mortality.
A total of 706 COVID-19 patients were included (349 treated with glucocorticoids, 357 not treated with glucocorticoids). After adjustment for relevant covariates, use of glucocorticoids was associated with a lower risk of in-hospital mortality (adjusted HR 0.44; 95% CI 0.26–0.72; p = 0.001). A significant interaction was observed between the protective effect of glucocorticoids on mortality and PaO2/FiO2 ratio on admission (p = 0.042), oxygen saturation on admission (p = 0.017), and peak CRP (0.023). Such protective effects of glucocorticoids were mainly observed in patients with lower PaO2/FiO2 ratio (<300), lower oxygen saturation (<90%), and higher CRP (>100 mg/L).
The protective effects of glucocorticoids on mortality in COVID-19 were more evident among patients with worse respiratory parameters and higher systemic inflammation.
Health care workers (HCWs) are prone to a heavy psycho-physical workload. Health promotion programs can help prevent the onset of chronic and work-related diseases. The aim of the STI-VI ...'before-and-after' study, with assessments scheduled at 6 and 12 months, was to improve the lifestyle of HCWs with at least one cardiovascular risk factor. A tailored motivational counseling intervention, focusing on dietary habits and physical activity (PA) was administered to 167 HCWs (53 males; 114 females). BMI, waist circumference, blood pressure, and cholesterol, triglyceride, and blood glucose levels were measured before and after the intervention. The 6-month results (total sample and by gender) showed a marked effect on lifestyle: PA improved (+121.2 MET,
= 0.01), and diets became more similar to the Mediterranean model (+0.8,
< 0.001). BMI dropped (-0.2,
< 0.03), and waist circumference improved even more (-2.5 cm;
< 0.001). Other variables improved significantly: total and LDL cholesterol (-12.8 and -9.4 mg/dL,
< 0.001); systolic and diastolic blood pressure (-4.4 and -2.5 mmHg,
< 0.001); blood glucose (-1.5 mg/dL,
= 0.05); and triglycerides (significant only in women), (-8.7 mg/dL,
= 0.008); but HDL cholesterol levels dropped too. If consolidated at 12 months, these results indicate that our intervention can help HCWs maintain a healthy lifestyle and work ability.
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