The design of a collagen scaffold containing iron oxide nanostructures capped by a TiO2 (anatase) layer is reported. The TiO2 shell is proposed to perform a dual role: 1) as an innovative and ...biocompatible cross‐linker agent, providing binding sites to the protein moiety, through the well‐known TiO2 chemical affinity towards carboxyl groups, and 2) as a protective surface layer for the paramagnetic core against oxidation. Simultaneously, the presence of the nanostructures confers to the collagen gel sensitivity to an external stimulus; that is, the application of a magnetic field. The hybrid biomaterial was demonstrated to be nontoxic and is proposed as a smart scaffold for the release of bioactive compounds on demand. The tuneable release of a model protein (myoglobin) upon application of a magnetic field was investigated. Myoglobin was loaded in the microporous material and discharge was induced by consecutive magnet applications, leading to release of the protein with high spatio–temporal and dosage control.
Find escape in a magnetic field: A collagen scaffold containing iron oxide nanostructures capped by a TiO2 (anatase) layer is reported. The nontoxic hybrid biomaterial can be used for the tuneable release of a model protein (myoglobin) upon application of a magnetic field (see figure).
Collagen-based matrices with axially oriented pores Madaghiele, Marta; Sannino, Alessandro; Yannas, Ioannis V. ...
Journal of biomedical materials research. Part A,
06/2008, Letnik:
85A, Številka:
3
Journal Article
Type I collagen is the most abundant protein of the human body. Due to its favourable properties, collagen extracted from animal tissues is adopted to manufacture a wide range of devices for ...biomedical applications. Compared to bovine and porcine collagens, which are the most largely used, equine collagen is free from the risk of zoonosis, has no reported immune reactions, and has not religious constraints. In this work, a recently available type I collagen extracted from horse tendon was evaluated and compared with a commercially available collagen isoform derived from the same species and tissue. Detailed physical, chemical and biological investigations were performed, in agreement with the requirements of the current standard for the characterization of type I collagen to be used for the manufacture of Tissue Engineering Medical Products. To the best of our knowledge, this is the first report on the complete primary structure of the investigated collagen.
In the present work, we investigated the potential of novel semi‐interpenetrating polymer network (semi‐IPN) cryogels, obtained through ultraviolet exposure of aqueous mixtures of poly(ethylene ...glycol) diacrylate and type I collagen, as tunable off‐the‐shelf platforms for 3D cancer cell research. We synthesized semi‐IPN cryogels with variable collagen amounts (0.1% and 1% w/v) and assessed the effect of collagen on key cryogel properties for cell culture, for example, porosity, degradation rate and mechanical stiffness. Then, we investigated the ability of the cryogels to sustain the long‐term growth of two pancreatic ductal adenocarcinoma (PDAC) cell populations, the parenchymal Panc1 cells and their derived cancer stem cells. Results revealed that both cell lines efficiently infiltrated, attached and expanded in the cryogels over a period of 14 days. However, only when grown in the cryogels with the highest collagen concentration, both cell lines reproduced their characteristic growth pattern previously observed in collagen‐enriched organotypic cultures, biomimetic of the highly fibrotic PDAC stroma. Cellular preembedding in Matrigel, that is, the classical approach to develop/grow organoids, interfered with an efficient intra‐scaffold migration and growth. Although preliminary, these findings highlight the potential of the proposed cryogels as reproducible and tunable cancer cell research platforms.
The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic ...commitment. The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68–87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold. Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface. Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture. However, scaffolds decorated with BMP-mimicking peptide show higher values of ALP than the biomineralized one. Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation. At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed. Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21. The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
Peripheral nerve injuries may lead to a significant function loss, which deeply affect patient's quality of life. In this context, tissue engineered collagen-based nerve guides are a promising ...alternative to autografts. To enhance the regeneration of the injured nerve tract, several bioactive molecules can be adopted as further components of the collagen-based conduits. Herein, sericin (Ser), a waste product of textile industry, was combined with type I collagen (Col), for the development of a bioactive substrate potentially able to support and enhance the regeneration of the peripheral nervous system. In particular, in order to identify an optimal substrate composition in terms of physicochemical and biological properties, thin Ser-Col films with different Ser:Col ratios were produced by air-drying and subsequently crosslinked through two different crosslinking methods. Then, Ser:Col films were characterized from a physicochemical point of view by examining secondary protein structure modifications via FTIR spectroscopy, swelling ratios, degradation rates and Ser release kinetics. Moreover, Ser:Col films ability to promote adhesion and proliferation of Schwann cells was evaluated in vitro. The results obtained in this study, although preliminary, suggest that Ser:Col blends could represent a promising strategy to enhance the repair of damaged peripheral nerves, by providing a sustained release of Ser from collagen-based nerve substrates.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
In the last two decades, marine collagen has attracted great scientific and industrial interest as a ‘blue resource’, with potential for use in various health-related sectors, such as food, medicine, ...pharmaceutics and cosmetics. In particular, the large availability of polluting by-products from the fish processing industry has been the key factor driving the research towards the conversion of these low cost by-products (e.g. fish skin and scales) into collagen-based products with high added value and low environmental impact. After addressing the extraction of collagen from aquatic sources and its physicochemical properties, this review focuses on the use of marine collagen and its derivatives (e.g. gelatin and peptides) in different healthcare sectors. Particular attention is given to the bioactive properties of marine collagen that are being explored in preclinical and clinical studies, and pave the way to an increased demand for this biomaterial in the next future. In this context, in addition to the use of native collagen for the development of tissue engineering or wound healing devices, particularly relevant is the use of gelatin and peptides for the development of dietary supplements and nutraceuticals, specifically directed to weight management and glycemic control. The marine collagen market is also briefly discussed to highlight the opportunities and the most profitable areas of interest.
•Growing scientific and industrial interest on marine collagen as a blue resource•Marine collagen improves wound healing and reduces inflammation.•Fish collagen enhances skin repairing processes and minimizes wrinkles.•Marine collagen is expected to show the fastest growth in the global collagen market.
Abstract Several bioengineering approaches have been proposed for peripheral nervous system repair, with limited results and still open questions about the underlying molecular mechanisms. We ...assessed the biological processes that occur after the implantation of collagen scaffold with a peculiar porous micro-structure of the wall in a rat sciatic nerve transection model compared to commercial collagen conduits and nerve crush injury using functional, histological and genome wide analyses. We demonstrated that within 60 days, our conduit had been completely substituted by a normal nerve. Gene expression analysis documented a precise sequential regulation of known genes involved in angiogenesis, Schwann cells/axons interactions and myelination, together with a selective modulation of key biological pathways for nerve morphogenesis induced by porous matrices. These data suggest that the scaffold's micro-structure profoundly influences cell behaviors and creates an instructive micro-environment to enhance nerve morphogenesis that can be exploited to improve recovery and understand the molecular differences between repair and regeneration.
PDF