The report contains a review of data in the Central Brassica Database and provision for further data transfer; review of methodologies for safety-duplication and regeneration; in situ conservation ...actions; strengths and weaknesses in Brassica genetic resources work in each country; thirteen country reports; and six papers in the GEN RES session, on developments in the Brassica project EU GEN RES CT99 109-112.
The Workshop was intended to be used to find ways of preventing loss of genetic resources and of counteracting the recurrent shortage of funds. The main concern related to the vegetatively propagated ...Allium collections was the high cost of conservation and the need to find solutions to ensure formal long-term commitments for the conservation of existing genetic diversity. Participants from the following countries presented the status of their collections: Czech Republic, Israel, Nordic countries, Germany, Poland, France, Portugal, UK, Netherlands, Spain, Bulgaria and Greece.
The European Cooperative Programme for Plant Genetic Resources (ECPGR) is a regional network funded by the European countries and coordinated by Bioversity International. The Vegetables Network with ...representatives of 42 countries, is one of the crop specific ECPGR networks (http://www.ecpgr.cgiar.org/ networks/vegetables.html). It consists of six Working Groups (WGs), i.e., on Allium, Brassica, Cucurbits, Leafy Vegetables, Solanaceae and Umbellifer Crops. Sharing responsibilities for the ex situ conservation of European vegetable crops genetic resources is the highest priority of the Vegetables Network. It is foreseen that the rationalization of the collections will lead to higher cost-efficiency, and improvement of plant genetic resources documentation and quality. These efforts will be continued within the framework of the AEGIS initiative (A European Genebank Integrated System) (http://www.aegis.cgiar.org/). Challenges for the Vegetables Network include the identification of the so-called Most Appropriate Accessions (MAA) for each crop for their inclusion in the decentrally managed European Collection, and the development of agreed crop specific technical standards for conservation. Achievements of the Network in recent years include the development of European Central Crop Databases (ECCDBs), quality standards for collection man-agement of seed-propagated crops and cryopreserved material, safety duplication improvement and definition of minimum characterization descriptors. Several EU-funded projects have initiated and accelerated the activities of the WGs. Apart from further improvements within the framework of AEGIS, the Network is planning a number of other initiatives, such as improving collaboration at the global level (Allium), developing molecular characterization protocols (lettuce), filling the gaps in the conservation of wild relatives (Brassica and Umbellifer Crops), and improving the Network’s communication with the scientific community and the public at large.
Proceedings of the meeting - briefing on EPGRIS (Establishment of a Plant Genetic Resources Infra-Structure) and EURISCO (European Internet Search Catalogue); presentations on national collections of ...Bulgaria, Hungary, Russian Federation and Turkey; adoption of FAO/IPGRI EURISCO Multi-crop passport descriptors for the European Arachis Database, Arachis characterization descriptors and the methodology for testing and describing accessions; recommendations and the work plan are reported. Country reports from Greece, Israel, Morocco, Portugal and Romania are also included in the publication besides papers of those presented in the meeting. Methodology for testing and description of accessions for the establishment of a database and minimum descriptor list for Arachis are included in the appendices
Part I of this publication is a summary report of the meeting and part II includes the papers presented. Papers are presented on the conservation strategies for medicinal and aromatic plants, ...sustainable use of medicinal and aromatic plants in Europe, and the origin of medicinal plants in Central Europe. Country reports from over twenty countries are presented. The design of the Central Database of the Slovene Plant Gene Bank is described. Research on molecular tools for determining genetic variability and genetic variability of native populations of Oregano in Slovenia is reviewed. A list of priority species/genera is included
The presentations given during the meeting covered the status of national collections, the status of the European Vitis Database (EVDB) and its descriptors, the problems of identification of ...grapevine varieties and reports of recent surveys on Vitis genetic resources.
At this meeting, the progress made by the group since 1996 was reported (in the organization of genetic resources management, computerization of data, documentation etc.) and also in the development ...of the central data base. Workplans and recommendations were drawn up for sharing of responsibilities, safety-duplication, rationalization of the collections etc. Papers were presented on the status of national collections in 27 European countries.
After a discussion on the International Flax Database and other European networks, papers were presented on the status of national collections in Bulgaria, Czech Republic, France, Germany, Hungary, ...Latvia, The Netherlands, Poland, Romania, Russia, Ukraine.
The histopathologic findings of mucosal epithelial cell injury in acute and chronic rejection in small bowel transplantation are well known. Several studies have been done on the pig either as ...autotransplantation, allotrans-plantation, orthotopic, or enterotopic transplantation, with or without immunosuppression.
Very few investigations on the enteroendocrine system have been done. In particular, none have been done on rejection in pigs after intestinal transplantation. On the other hand, changes in the enteroendocrine markers (somatostatin and chromogranin A) have been examined in detail after auxiliary small bowel transplantation in dogs without immunosuppression, showing no apparent changes on the 7th postoperative day, even if in a severe rejection status.
Recent studies on rats have evaluated the presence of serotonin after small bowel allotransplantation with different times of preservation, and after reperfusion. The authors concluded that the number of serotonin-positive cells decreased with a preservation time of more than 1 hour in Ringer lactate at 4°C.
We too have examined the modifications of argentaffin as well as serotonin-positive cells in the harvested small bowel of pigs perfused with Belzer solution (4°C) and preserved in Ringer lactate at 4°C, after preservation and reperfusion in the combined liver-small bowel allotransplantation. We noticed that the argentaffin and serotonin cells were preserved up to 20 hours and that after reperfusion the most important injury was due to the disappearance of these cells from the crypts.
In this study, we studied the modifications of the intestinal enterochromaffin cells after combined liver-small bowel and small bowel alone allotransplantation in pigs, with the aim of evaluating a correlation with rejection.
