Extinction risk and threats to plants and fungi Nic Lughadha, Eimear; Bachman, Steven P.; Leão, Tarciso C. C. ...
Plants, people, planet,
September 2020, Letnik:
2, Številka:
5
Journal Article
Recenzirano
Odprti dostop
There is increasing awareness that plants and fungi, as natural solutions, can play an important role in tackling ongoing global environmental challenges. We illustrate how understanding current and ...projected threats to plants and fungi is necessary to manage and mitigate risks, and how building awareness and understanding of gaps and bias in current assessment coverage is essential to prioritize conservation efforts. We highlight the state of the art in conservation science and point to current methods of assessment and future studies needed to mitigate species extinction
Societal Impact Statement
There is increasing awareness that plants and fungi, as natural solutions, can play an important role in tackling ongoing global environmental challenges. We illustrate how understanding current and projected threats to plants and fungi is necessary to manage and mitigate risks, while building awareness of gaps and bias in current assessment coverage is essential to adequately prioritize conservation efforts. We highlight the state of the art in conservation science and point to current methods of assessment and future studies needed to mitigate species extinction.
Summary
Plant and fungal biodiversity underpin life on earth and merit careful stewardship in an increasingly uncertain environment. However, gaps and biases in documented extinction risks to plant and fungal species impede effective management. Formal extinction risk assessments help avoid extinctions, through engagement, financial, or legal mechanisms, but most plant and fungal species lack assessments. Available global assessments cover c. 30% of plant species (ThreatSearch). Red List coverage overrepresents woody perennials and useful plants, but underrepresents single‐country endemics. Fungal assessments overrepresent well‐known species and are too few to infer global status or trends. Proportions of assessed vascular plant species considered threatened vary between global assessment datasets: 37% (ThreatSearch), and 44% (International Union for Conservation of Nature Red List of Threatened Species). Our predictions, correcting for several quantifiable biases, suggest that 39% of all vascular plant species are threatened with extinction. However, other biases remain unquantified, and may affect our estimate. Preliminary trend data show plants moving toward extinction. Quantitative estimates based on plant extinction risk assessments may understate likely biodiversity loss: they do not fully capture the impacts of climate change, slow‐acting threats, or clustering of extinction risk, which could amplify loss of evolutionary potential. The importance of extinction risk estimation to support existing and emerging conservation initiatives is likely to grow as threats to biodiversity intensify. This necessitates urgent and strategic expansion of efforts toward comprehensive and ongoing assessment of plant and fungal extinction risk.
Background The use of postmastectomy reconstruction varies with socioeconomic status, but the etiology of these variations is not understood. We investigated whether these differences reflect ...variations in the rate or qualitative aspects of the provider’s discussion of reconstruction as an option. Study Design Data were collected through chart review and patient survey for stages I to III breast cancer patients during the National Initiative on Cancer Care Quality. Multivariable logistic regression was used to identify predictors of reconstruction and discussion of reconstruction as an option. Predictors of not receiving reconstruction despite a documented discussion were also determined. Results There were 253 of 626 patients who received reconstruction (40.4%). Younger, more educated Caucasian women who were not overweight or receiving postmastectomy radiation were more likely to receive reconstruction. Patients who were younger, more educated, and not receiving postmastectomy radiation were more likely to have a documented discussion of reconstruction. If a discussion was documented, patients who were older, Hispanic, not born in the US, and received postmastectomy radiation were less likely to receive reconstruction. The greatest predictor of reconstruction was medical record documentation of a discussion about reconstruction. Conclusions We observed disparities in the likelihood of reconstruction that were at least partially explained by differences in the likelihood that reconstruction was discussed. But there were also differences in the likelihood of reconstruction based on age, race, and radiation once discussions occurred. Efforts to increase and improve discussions about reconstruction may decrease disparities for this procedure.
High-resolution images of the south polar residual cap of Mars acquired in 1999 and 2001 show changes in the configuration of pits, intervening ridges, and isolated mounds. Escarpments have retreated ...1 to 3 meters in 1 martian year, changes that are an order of magnitude larger than can be explained by the sublimation of water ice, but close to what is expected for sublimation of carbon dioxide ice. These observations support a 35-year-old conjecture that Mars has a large surface reservoir of solid carbon dioxide. The erosion implies that this reservoir is not in equilibrium with the present environment and that global climate change is occurring on Mars.
We have used intramolecular cross-linking, MS, and sequence threading to rapidly identify the fold of a model protein, bovine basic fibroblast growth factor (FGF)-2. Its tertiary structure was probed ...with a lysine-specific cross-linking agent, bis(sulfosuccinimidyl) suberate (BS3). Sites of cross-linking were determined by tryptic peptide mapping by using time-of-flight MS. Eighteen unique intramolecular lysine (Lys-Lys) cross-links were identified. The assignments for eight cross-linked peptides were confirmed by using post source decay MS. The interatomic distance constraints were all consistent with the tertiary structure of FGF-2. These relatively few constraints, in conjunction with threading, correctly identified FGF-2 as a member of the β -trefoil fold family. To further demonstrate utility, we used the top-scoring homolog, IL-1β , to build an FGF-2 homology model with a backbone error of 4.8 angstrom (rms deviation). This method is fast, is general, uses small amounts of material, and is amenable to automation.
Wave modeling and analysis of sedimentary structures were used to evaluate whether four examples of symmetrical, reversing, or straight‐crested bedforms in Gale crater sandstones are preserved wave ...ripples; deposition by waves would demonstrate that the lake was not covered by ice at that time. Wave modeling indicates that regardless of atmospheric density, winds that exceeded the threshold of aeolian sand transport could have generated waves capable of producing nearshore wave ripples in most grain sizes of sand. Reversing 3‐m‐wavelength bedforms in the Kimberley formation are interpreted not as wave ripples but rather as large aeolian ripples that formed in an atmosphere approximately as thin as at present. These exhumed bedforms define many of the ridges at outcrops that appear striated in satellite images. At Kimberley these bedforms demonstrably underlie and therefore predate subaqueous beds, suggesting that a thin atmosphere existed at least temporarily before subaqueous deposition ceased in the crater. The other three candidate wave ripples (Square Top, Hunda, and Voe) are consistent with modeled waves, but other origins cannot be excluded. The predominance of flat‐laminated (non‐rippled) beds in the lacustrine Murray formation suggests that some aspect of the lake was not conducive to formation or preservation of recognizable wave ripples. Water depths may generally have been too deep, lakebed sediment may have been too fine‐grained, the lake may have been smaller than modeled, or the lake may have been covered by ice.
Plain Language Summary
Wave modeling and analysis of sedimentary structures were used to evaluate whether ancient lake deposits in Gale crater contain ripples formed by waves on the surface of the lake. Deposition by waves would show that the lake was not covered by ice at that time. Modeling shows that regardless of atmospheric density, winds capable of moving sand on land would generally have been strong enough to form waves that would produce ripples near shore. Large bedforms in the Kimberley formation are interpreted as ripples formed by the wind in an atmosphere similar to that of Mars today. These bedforms underlie and are older than other beds deposited in water, thereby showing that a thin atmosphere existed at least temporarily before deposition in water ceased in the crater. Three other candidate wave ripples are consistent with modeled waves, but other origins are possible. Thick sequences of sedimentary rock in Gale crater are flat‐laminated rather than rippled, suggesting that some aspect of the lake was not favorable for their formation or preservation. Much of the lake may have been too deep or ice‐covered, or the lake may have been smaller than modeled or had sediment too fine to form easily observed ripples.
Key Points
Wave modeling was used to test the hypothesis that 4 examples of lithified bedforms observed by the Curiosity rover are wave ripples
3‐m‐wavelegnth bedforms are thin‐atmosphere aeolian ripples formed before the last subaqueous deposition ended in Gale crater
Three examples remain viable candidates for wave ripples‐which would indicate a lake that was largely free of ice at time of deposition
Viruses cleave cellular proteins to remodel the host proteome. The study of these cleavages has revealed mechanisms of immune evasion, resource exploitation, and pathogenesis. However, the full ...extent of virus-induced proteolysis in infected cells is unknown, mainly because until recently the technology for a global view of proteolysis within cells was lacking. Here, we report the first comprehensive catalog of proteins cleaved upon enterovirus infection and identify the sites within proteins where the cleavages occur. We employed multiple strategies to confirm protein cleavages and assigned them to one of the two enteroviral proteases. Detailed characterization of one substrate, LSM14A, a p body protein with a role in antiviral immunity, showed that cleavage of this protein disrupts its antiviral function. This study yields a new depth of information about the host interface with a group of viruses that are both important biological tools and significant agents of disease.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Cellular senescence, an irreversible proliferative cell arrest, is caused by excessive intracellular or extracellular stress/damage. Increased senescent cells have been identified in multiple tissues ...in different metabolic and other aging-related diseases. Recently, several human and mouse studies emphasized the involvement of senescence in development and progression of NAFLD. Hyperinsulinemia, seen in obesity, metabolic syndrome, and other conditions of insulin resistance, has been linked to senescence in adipocytes and neurons. Here, we investigate the possible direct role of chronic hyperinsulinemia in the development of senescence in human hepatocytes.
Using fluorescence microscopy, immunoblotting, and gene expression, we tested senescence markers in human hepatocytes subjected to chronic hyperinsulinemia in vitro and validated the data in vivo by using liver-specific insulin receptor knockout (LIRKO) mice. The consequences of hyperinsulinemia were also studied in senescent hepatocytes following doxorubicin as a model of stress-induced senescence. Furthermore, the effects of senolytic agents in insulin- and doxorubicin-treated cells were analyzed.
Results showed that exposing the hepatocytes to prolonged hyperinsulinemia promotes the onset of senescence by increasing the expression of p53 and p21. It also further enhanced the senescent phenotype in already senescent hepatocytes. Addition of insulin signaling pathway inhibitors prevented the increase in cell senescence, supporting the direct contribution of insulin. Furthermore, LIRKO mice, in which insulin signaling in the liver is abolished due to deletion of the insulin receptor gene, showed no differences in senescence compared to their wild-type counterparts despite having marked hyperinsulinemia indicating these are receptor-mediated effects. In contrast, the persistent hyperinsulinemia in LIRKO mice enhanced senescence in white adipose tissue. In vitro, senolytic agents dasatinib and quercetin reduced the prosenescent effects of hyperinsulinemia in hepatocytes.
Our findings demonstrate a direct link between chronic hyperinsulinemia and hepatocyte senescence. This effect can be blocked by reducing the levels of insulin receptors or administration of senolytic drugs, such as dasatinib and quercetin.
•Chronic hyperinsulinemia promotes human hepatocyte senescence via the p53/p21 pathway.•Hyperinsulinemia-induced senescence requires the PI3K/AKT/mTOR signaling pathway.•Hyperinsulinemia makes human hepatocytes more susceptible to stress-induced senescence.•Insulin sustains pro-senescence cellular stress by enhancing Cyclin D1 expression.•Dasatinib and quercetin inhibit the hyperinsulinemia-induced hepatocyte senescence.
Earlier studies have provided varying risk estimates for lymphoma in patients with inflammatory bowel disease (IBD), but often have been limited by detection biases (especially during the first year ...of follow-up evaluation), misclassification, and small sample size; and rarely reflect modern-day management of IBD.
We performed a binational register-based cohort study (Sweden and Denmark) from 1969 to 2019. We compared 164,716 patients with IBD with 1,639,027 matched general population reference individuals. Cox regression estimated hazard ratios (HRs) for incident lymphoma by lymphoma subtype, excluding the first year of follow-up evaluation.
From 1969 to 2019, 258 patients with Crohn’s disease (CD), 479 patients with ulcerative colitis (UC), and 6675 matched reference individuals developed lymphoma. This corresponded to incidence rates of 35 (CD) and 34 (UC) per 100,000 person-years in IBD patients, compared with 28 and 33 per 100,000 person-years in their matched reference individuals. Although both CD (HR, 1.32; 95% CI, 1.16–1.50) and UC (HR, 1.09; 95% CI, 1.00–1.20) were associated with an increase in lymphoma, the 10-year cumulative incidence difference was low even in CD patients (0.08%; 95% CI, 0.02–0.13). HRs have increased in the past 2 decades, corresponding to increasing use of immunomodulators and biologics during the same time period. HRs were increased for aggressive B-cell non-Hodgkin lymphoma in CD and UC patients, and for T-cell non-Hodgkin lymphoma in CD patients. Although the highest HRs were observed in patients exposed to combination therapy (immunomodulators and biologics) or second-line biologics, we also found increased HRs in patients naïve to such drugs.
During the past 20 years, the risk of lymphomas have increased in CD, but not in UC, and were driven mainly by T-cell lymphomas and aggressive B-cell lymphomas.
The Thermal Emission Imaging System (THEMIS) on 2001 Mars Odyssey will investigate the surface mineralogy and physical properties of Mars using multi-spectral thermal-infrared images in nine ...wavelengths centered from 6.8 to 14.9 mm, and visible/near-infrared images in five bands centered from 0.42 to 0.86 mm. THEMIS will map the entire planet in both day and night multi-spectral infrared images at 100-m per pixel resolution, 60% of the planet in one-band visible images at 18-m per pixel, and several percent of the planet in 5-band visible color. Most geologic materials, including carbonates, silicates, sulfates, phosphates, and hydroxides have strong fundamental vibrational absorption bands in the thermal-infrared spectral region that provide diagnostic information on mineral composition. The ability to identify a wide range of minerals allows key aqueous minerals, such as carbonates and hydrothermal silica, to be placed into their proper geologic context. The specific objectives of this investigation are to: (I) determine the mineralogy and petrology of localized deposits associated with hydrothermal or sub-aqueous environments, and to identify future landing sites likely to represent these environments; (2) search for thermal anomalies associated with active sub-surface hydrothermal systems; (3) study small-scale geologic processes and landing site characteristics using morphologic and thermophysical properties; and (4) investigate polar cap processes at all seasons. THEMIS follows the Mars Global Surveyor Thermal Emission Spectrometer (TES) and Mars Orbiter Camera (MOC) experiments, providing substantially higher spatial resolution IR multi-spectral images to complement TES hyperspectral (143-band) global mapping, and regional visible imaging at scales intermediate between the Viking and MOC cameras. The THEMIS uses an uncooled microbolometer detector array for the IR focal plane. The optics consists of all-reflective, three-mirror anastigmat telescope with a 12-cm effective aperture and a speed of f/1.6. The IR and visible cameras share the optics and housing, but have independent power and data interfaces to the spacecraft. The IR focal plane has 320 cross-track pixels and 240 downtrack pixels covered by 10 --1-p.m-bandwidth strip filters in nine different wavelengths. The visible camera has a 1024 x 1024 pixel array with 5 filters. The instrument weighs 11.2 kg, is 29 cm by 37 cm by 55 cm in size, and consumes an orbital average power of 14 W.
The Adhesion G-protein-coupled receptors (GPCRs) are the most complex gene family among GPCRs with large genomic size, multiple introns, and a fascinating flora of functional domains, though the ...evolutionary origin of this family has been obscure. Here we studied the evolution of all class B (7tm2)-related genes, including the Adhesion, Secretin, and Methuselah families of GPCRs with a focus on nine genomes. We found that the cnidarian genome of Nematostella vectensis has a remarkably rich set of Adhesion GPCRs with a broad repertoire of N-terminal domains although this genome did not have any Secretin GPCRs. Moreover, the single-celled and colony-forming eukaryotes Monosiga brevicollis and Dictyostelium discoideum contain Adhesion-like GPCRs although these genomes do not have any Secretin GPCRs suggesting that the Adhesion types of GPCRs are the most ancient among class B GPCRs. Phylogenetic analysis found Adhesion group V (that contains GPR133 and GPR144) to be the closest relative to the Secretin family in the Adhesion family. Moreover, Adhesion group V sequences in N. vectensis share the same splice site setup as the Secretin GPCRs. Additionally, one of the most conserved motifs in the entire Secretin family is only found in group V of the Adhesion family. We suggest therefore that the Secretin family of GPCRs could have descended from group V Adhesion GPCRs. We found a set of unique Adhesion-like GPCRs in N. vectensis that have long N-termini containing one Somatomedin B domain each, which is a domain configuration similar to that of a set of Adhesion-like GPCRs found in Branchiostoma floridae. These sequences show slight similarities to Methuselah sequences found in insects. The extended class B GPCRs have a very complex evolutionary history with several species-specific expansions, and we identified at least 31 unique N-terminal domains originating from other protein classes. The overall N-terminal domain structure, however, concurs with the phylogenetic analysis of the transmembrane domains, thus enabling us to track the origin of most of the subgroups.
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