The aim of this study was to investigate the presence of heavy metals/metalloids (Pb, Cd, Hg, Cu, Fe, Zn, As) in the muscle tissue of fish from the Danube River (two locations: Zemun and Grocka). For ...the purpose of heavy metal determination in fish muscle, 120 samples of six different fish species, Prussian carp, barbel, bream, carp, pike perch, and catfish were collected. For determining heavy metals, we used microwave oven digestion and atomic absorption spectrometer methods. The highest average content of Pb (0.084 ± 0.004 mg kg
−1
), Cd (0.082 ± 0.003 mg kg
−1
), Hg (0.466 ± 0.006 mg kg
−1
), and As (0.333 ± 0.007 mg kg
−1
) was found in the muscle of carp (an omnivorous fish) from Grocka, while the highest average level of Fe (13.60 ± 0.03 mg kg
−1
) was deposited in bream (also omnivorous) from Zemun. Also, the average Cu level (1.62 ± 0.13 mg kg
−1
) was the highest in catfish muscle (a carnivorous fish) from Grocka, while the highest Zn content (11.16 ± 0.17 mg kg
−1
) was determined in muscle of Prussian carp (an omnivorous fish) from Zemun. The highest content of heavy metals (Cu, Fe, and Zn, respectively) in muscle of the six different types of fish from both locations was symmetrically arranged by species (catfish, barbel, and Prussian carp, respectively). Concentrations of Pb, Hg, and As in the Danube River fish muscle were under the maximum residual levels prescribed by the European Union (EU) and the maximum allowed concentrations (MAC) for Serbia. On the other hand, in all fish muscle from both locations (Zemun and Grocka), higher concentrations of Cd than prescribed (MAC) were found, with the exception of bream and pike perch.
The aim of this study was to investigate the presence of metal contamination in water, sediments and three different fish species. All samples were taken from the Danube River in Belgrade Region, a ...location upstream from Grocka. Concentrations of Cd, Hg and Pb in water samples were not detected, while concentrations of Zn, Fe, Cu and As were in the range of 0.004–0.41 mg L
−1
. Iron was the most deposited metal in sediment samples (17,530.00 mg kg
−1
). For the purpose of heavy metal determination in fish tissue, silver carp, common carp and wels catfish were collected. Concentrations of Pb, Cd and As were determined in muscle, digestive tract and liver by inductively coupled plasma-optical emission spectrophotometry (IPC-OES). The highest concentration of Pb was in the digestive tract of all three fish species, while Cd was mostly deposited in the liver. The highest concentration of Hg was in the muscle tissue of wels catfish, and these values are above the maximum residual levels prescribed by the European Union and the maximum allowed concentrations (MACs) for Serbia. Concentration of As was mostly deposited in the liver, but under the MAC.
Robule Lake, located in Eastern Serbia, near the city of Bor, is being influenced by waste materials from mining activities. For the purification of water from Robule Lake, contaminated with various ...metal ions (Fe, Cu, Zn, Mn, Cd, Ni, etc.), acid mine drainage (AMD) treatment methods such as: passive treatment method, sequential neutralization, ion exchange, adsorption process based on low cost adsorbents, adsorption process based on natural zeolits, electrodialysis, filtration with nanofiltration membranes, and reverse osmosis, were evaluated by the following MCDM methods: TOPSIS, VIKOR, MOOSRA, WASPAS, and CoCoSo. Criteria used for the evaluation were: efficiency in the metal ions removal and the quality of the purified water, necessity of pre-treatment and / or post-treatment of treated water, possibility of using the generated waste, capital costs, operating and maintenance costs, needed area, and sensitivity of the method. The results of the MCDM analysis showed that sequential neutralization was the most appropriate method for this wastewater, while passive treatment system and ion exchange were ranked as second and third, respectively.
After the selection of AMD treatment method, neutralization tests with lime were carried out with the water sample from Robule Lake. The results of sequential neutralization testing showed that concentration of Fe ions could be lowered to 1 mg/L at pH 4. The values of Cu, Zn, and Ni ions concentration obtained at pH 7 were 0.04, 0.65, and 0.21 mg/L, respectively, while the values of Mn and Cd ions concentration of 0.01 and 0.0001 mg/L, were obtained at pH 10.
Highly porous lignin-based microspheres, modified by magnetite nanoparticles, were used for the first time for the removal of selenate anions, Se(VI), from spiked and real water samples. The ...influence of experimental conditions: selenate concentration, adsorbent dosage and contact time on the adsorption capacity was investigated in a batch experimental mode. The FTIR, XRD, SEM techniques were used to analyze the structural and morphological properties of the native and exhausted adsorbent. The maximum adsorption capacity was found to be 69.9 mg/g for Se(VI) anions at pH 6.46 from the simulated water samples. The modified natural polymer was efficient in Se(VI) removal from the real (potable) water samples, originated from six cities in the Republic of Serbia, with an overage efficacy of 20%. The regeneration capacity of 61% in one cycle of desorption (0.5 M NaOH as desorption solution) of bio-based adsorbent was gained in this investigation. The examined material demonstrated a significant affinity for Se(VI) oxyanion, but a low potential for multi-cycle material application; consequently, the loaded sorbent could be proposed to be used as a Se fertilizer.
Today, a few differing sources of selenium (Se), i.e. inorganic, organic, and nano forms of Se, are used as feed supplements for poultry. Published research indicates that nano-Se and organic Se ...possess comparable efficiency to inorganic Se in increasing GSH-Px activity of plasma and various tissues, but they deposit at higher rates in various tissues. However, there are principal differences in absorption mechanisms, metabolism, and efficiency of these three forms of Se. The aim of this review was to analyze the available literature on the effects of different Se sources and levels in the diet on glutathione peroxidase (GSH-Px) activity, tissue Se distribution and growth performance in poultry. Higher levels of Se increase GSH-Px activity in the body, but this reaches a plateau even if Se concentrations in diet increase further, while the deposition of Se in tissues increases as Se content in diet increases. In addition, many studies have shown the positive effects of adding Se to diet on growth performance in poultry. Optimal Se supplementation is necessary not only for good poultry health but also to ensure and preserve meat quality during storage and to provide human beings with this microelement.
Abstract
The present study was conducted to assess effects of selenium (Se)-yeast supplementation on glutathione peroxidase activity, Se levels in tissues, growth performance, carcass, and meat ...composition in broilers. A total of 275 one-d-old Cobb 500 broilers of both sexes were randomly allotted to 1 of 5 treatments during a 42-d period. The 5 treatments differed only in Se content: group 1 had no additional Se (background only); groups 2, 3, and 4 received 0.3 mg/kg of added Se from the beginning of the trial until d 21, whereas in the second half of the study (from d 22 to 42), these groups received 0.3, 0.6, and 0.9 mg/kg of added Se, respectively; and group 5 received 0.9 mg/kg of Se for the entire experimental period. At the end of the study, the control group showed significantly lower (P < 0.01) glutathione peroxidase activity in blood plasma compared to Se-supplemented groups. Regarding Se concentration in various tissues, the groups receiving Se yeast showed higher plasma, feces, and meat Se contents than the control group (P < 0.01). Supplementation of Se improved broilers' body weight, weight gain and feed conversion ratio (P < 0.01). Dressing percentage was lower in the control group and the group with 0.3 mg/kg of added Se compared to other experimental groups (0.6 and 0.9 mg/kg of dietary Se). The proportion of less valuable carcass parts (wings and legs) was higher (P < 0.01) in the group fed the basal diet compared to groups supplemented with 0.9 mg/kg of Se. Initial and ultimate pH values differed among experimental groups (P < 0.05). Supplementation of Se improved the broiler's antioxidative resistance, growth performance, carcass quality, and chemical composition of meat.
The aim of this experiment was to investigate the effects of benzoic acid and chelates in which copper (Cu), zinc (Zn) and manganese (Mn) were bound to methionine hydroxy analogue on growth ...performance, intestinal morphology, intestinal microbiota and digesta pH value of post-weaning piglets at 28 days of age. The experiment was conducted on 96 piglets randomly assigned to one of four treatments (6 replicate pens of 4 piglets each): 1) control (C) – microminerals were provided as sulfates of Cu, Zn and Mn at 130 (80 at second phase), 100, 120 mg/kg in the first phase, respectively; 2) chelates (CTM), microelements were provided as chelates of Cu, Zn and Mn at 130 (80 in second phase), 60, 60 mg/kg in the first phase, respectively; 3) benzoic acid (BA), with the addition of 2500 mg/kg during both periods; 4) chelates + benzoic acid (CTM + BA), microelements were provided as chelates of Cu, Zn and Mn at 130 (80 in second phase), 60, 60 mg/kg in the first phase, respectively, and 2500 mg/kg of benzoic acid during both periods. Results showed that chelates and benzoic acid supplementation not only improved the final body weight (p<0.05), average daily gain (p<0.05) and feed conversion ratio (p<0.05), but also increased the morphology performance and decreased the number of
in the jejunum and ileum in the treated groups (p<0.05). This study provides the evidence that dietary supplementation has beneficial effects on the intestinal morphology and microflora of weaned pigs, which can partly explain why growth performance of the piglets was improved.
This paper considers the impact of copper mining-influenced water and metallurgical wastewater on the surface water in the Bor area, Serbia. Sampling, realized through the four campaigns (2020–2021), ...confirmed that both types of water, discharged without appropriate treatment in the Bor River, had a signific impact on the concentration of metal ions, pH and electrical conductivity on the watercourse in the Bor area. The highest concentrations of the following metal ions, Cu—271 mg/L, As—25,991 μg/L, Ni—13,856 μg/L, Cd—2627 μg/L, and Pb—2855 μg/L, were registered in the metallurgical wastewater samples. After changes occurred in the copper production process by stopping the discharge of untreated wastewater into the Bor River, the concentrations of monitored elements were drastically decreased. In the period 2022–2024, the concentration values for Cu, As and Pb ions were below the maximum allowable value, and the concentration values of Ni and Cd ions were also decreased. The values for pH and electrical conductivity were in the maximum allowable range. The return of wastewater to the copper production process would lead to both a reduction in the primary water consumption and reduction in the negative impact on the environment.
Anisakis allergy in human Ivanović, Jelena; Baltić, Milan Ž.; Bošković, Marija ...
Trends in food science & technology,
January 2017, 2017-01-00, 20170101, Letnik:
59
Journal Article
Recenzirano
Several case reports show allergy and anaphylactic reactions to the fish parasite Anisakis in the domestic and occupational setting. Compared with other well-studied parasitic diseases, fish-borne ...parasitic zoonoses do not get enough attention, especially because these zoonoses have been limited for the most part to populations living in middle-income countries. Human fishery product-borne parasitic diseases caused by nematodes result in infection following ingestion of viable parasites, or as allergic reactions against parasite antigens. With the globalization of the seafood industry, the risk of humans acquiring anisakiasis in developed countries appears to be underestimated. The risk of infection with Anisakis simplex and related parasites of fish has been recognized for some time, but it is now emerging that indigestion of material from dead parasites in food also present a danger. The resulting allergic reactions range from rapid onset and potentially lethal anaphylactic reaction to chronic, debilitating conditions. This review discusses the importance of infection with Anisakis simplex and potential of allergy in human.
•Food allergy increase in world.•Anisakis simplex increase is food-borne nematode.•Human infected by Anisakis simplex by eating raw fish.•Infection with Anisakis simplex often results in allergic host immune response.
Three different forms of Cu powder particles obtained by either galvanostatic electrolysis or a non-electrolytic method were analyzed by a scanning electron microscope (SEM), X-ray diffraction (XRD) ...and particle size distribution (PSD). Electrolytic procedures were performed under different hydrogen evolution conditions, leading to the formation of either 3D branched dendrites or disperse cauliflower-like particles. The third type of particles were compact agglomerates of the Cu grains, whose structural characteristics indicated that they were formed by a non-electrolytic method. Unlike the sharp tips that characterize the usual form of Cu dendrites, the ends of both the trunk and branches were globules in the formed dendrites, indicating that a novel type of Cu dendrites was formed in this investigation. Although the macro structures of the particles were extremely varied, they had very similar micro structures because they were constructed by spherical grains. The Cu crystallites were randomly oriented in the dendrites and compact agglomerates of the Cu grains, while the disperse cauliflower-like particles showed (220) and (311) preferred orientation. This indicates that the applied current density affects not only the morphology of the particles, but also their crystal structure. The best performance, defined by the largest specific surface area and the smallest particle size, was by the galvanostatically produced powder consisting of disperse cauliflower-like particles.