Adoptive immunotherapy with tumour-infiltrating lymphocytes (TIL) may benefit from the use of selective markers, such as PD-1, for tumour-specific T-cell enrichment, and the identification of ...predictive factors that help identify those patients capable of rendering tumour-reactive TILs. We have investigated this in ovarian cancer (OC) patients as candidates for TIL therapy implementation.
PD-1
and PD-1
CD8 TILs were isolated from ovarian tumours and expanded cells were tested against autologous tumour cells. Baseline tumour samples were examined using flow cytometry, multiplexed immunofluorescence and Nanostring technology, for gene expression analyses, as well as a next-generation sequencing gene panel, for tumour mutational burden (TMB) calculation.
Tumour-reactive TILs were detected in half of patients and were exclusively present in cells derived from the PD-1
fraction. Importantly, a high TIL density in the fresh tumour, the presence of CD137
cells within the PD-1
CD8
TIL subset and their location in the tumour epithelium, together with a baseline T-cell-inflamed genetic signature and/or a high TMB, are features that identify patients rendering tumour-reactive TIL products.
We have demonstrated that PD-1 identifies ovarian tumour-specific CD8 TILs and has uncovered predictive factors that identify OC patients who are likely to render tumour-specific cells from PD-1
TILs.
The integral urban water cycle of the Balearic Islands (Spain) is composed of desalination plants, extraction wells, water distribution networks, treatment plants and sewerage systems. This article ...presents the results of the carbon and water footprint of each of the islands that form the Balearic archipelago, finding differences between those islands with a greater contribution of groundwater, such as Mallorca, and those that are fed exclusively with desalinated water, such as Formentera. Water consumption on these islands is highly seasonal, which results in peaks in demand, which are mainly supplied by desalination. This article aims to be the starting point for assessing the water and energy status of the facilities related to drinking water consumption in the archipelago to be able to take measures aimed at ecological transition in this sector. The results obtained show that seawater desalination plants have the largest carbon footprint, mainly due to their high electricity consumption.
Phelan-McDermid syndrome (PMS, OMIM# 606232) results from either different rearrangements at the distal region of the long arm of chromosome 22 (22q13.3) or pathogenic sequence variants in the
gene.
...codes for a structural protein that plays a central role in the formation of the postsynaptic terminals and the maintenance of synaptic structures. Clinically, patients with PMS often present with global developmental delay, absent or severely delayed speech, neonatal hypotonia, minor dysmorphic features, and autism spectrum disorders (ASD), among other findings. Here, we describe a cohort of 210 patients with genetically confirmed PMS. We observed multiple variant types, including a significant number of small deletions (<0.5 Mb, 64/189) and
sequence variants (21 cases). We also detected multiple types of rearrangements among microdeletion cases, including a significant number with post-zygotic mosaicism (9.0%, 17/189), ring chromosome 22 (10.6%, 20/189), unbalanced translocations (
or inherited, 6.4%), and additional rearrangements at 22q13 (6.3%, 12/189) as well as other copy number variations in other chromosomes, unrelated to 22q deletions (14.8%, 28/189). We compared the clinical and genetic characteristics among patients with different sizes of deletions and with
variants
Our findings suggest that
plays an important role in this syndrome but is probably not uniquely responsible for all the spectrum features in PMS. We emphasize that only an adequate combination of different molecular and cytogenetic approaches allows an accurate genetic diagnosis in PMS patients. Thus, a diagnostic algorithm is proposed.
This article covers ten years of GRB follow-ups by the Spanish BOOTES stations: 71 follow-ups providing 23 detections. Follow-ups by BOOTES-1B from 2005 to 2008 were given in a previous article and ...are here reviewed and updated, and additional detection data points are included as the former article merely stated their existence. The all-sky cameras CASSANDRA have not yet detected any GRB optical afterglows, but limits are reported where available.
Se realizó la presentación de un caso con un tumor de células gigantes en el extremo distal del fémur derecho a nivel del cóndilo femoral interno. Se retira quirúrgicamente, previo estudio, donde se ...confirmó el diagnóstico a través de la biopsia. En dicho proceder se efectuó un raspado extenso de la lesión respetando la superficie articular del cóndilo femoral, rellenando la cavidad con la hidroxiapatita HAP-200 ®, sin necesidad de apoyar dicho acto con una osteosíntesis interna o externa, sólo con una calza de yeso por 6 semanas. El seguimiento del paciente ha sido, hasta la fecha, de 6 años y no se ha reportado ninguna recidiva o metástasis, con una osteointegración positiva del biomaterial, lográndose la curación ósea y una función articular sin restricción.
Introducción: las fracturas de tibia producidas por mecanismo de alta energía pueden provocar áreas de defectos, que llevan a pseudoartrosis o infección ósea. Por esto, se buscan nuevas alternativas ...para la solución de estos defectos óseos, entre ellos el uso del biomaterial hidroxiapatita coralina porosa HAP-200® como andamiaje en el tratamiento de estas fracturas. Objetivos: describir la eficacia de la HAP-200® como andamiaje. Material y métodos: se realizó un estudio de tipo descriptivo, longitudinal, que incluyó la totalidad de pacientes (13) atendidos por fracturas complejas de la tibia, desde enero de 1994 hasta el 2010. Fueron tratados con diversos materiales de osteosíntesis e injerto de HAP-200® como andamiaje en la zona de defecto óseo, y evaluados evolutivamente por consulta durante 10 años. En el estudio fueron empleadas las clasificaciones del Sistema Asociación para el Estudio de la Fijación Interna y la de Gustilo y Anderson en las fracturas abiertas; y las clasificaciones del Sistema Asociación para el Estudio de la Fijación Interna y la de Tscherne y Gotze para la evaluación de partes blandas en las fracturas cerradas. Se confeccionó una base de datos mediante el paquete estadístico SPSS 1.14, para cumplir los objetivos trazados. Se realizó el consentimiento informado a cada paciente. Resultados: el 76,9 % correspondió al sexo masculino. Se logró la curación ósea en el 92,3 %. El período de curación tuvo una duración media de 16 semanas en las fracturas cerradas, y de 20 semanas en las expuestas. Conclusiones: los resultados demuestran la validez e importancia de la HAP-200® como un biomaterial osteoconductor.
Se exponen los resultados obtenidos en el tratamiento de las fracturas inestables del extremo distal del radio, en una muestra de 55 pacientes. La reducción de las fracturas se realizó mediante ...tracción digital, con previa anestesia regional del plexo braquial. Se emplearon diversos métodos de osteosíntesis, el más frecuente fue el fijador externo (41 casos) y se empleó alambres bloqueantes en los casos que lo requirieron. Se aplicó implantes de hidroxiapatita coralina HAP-200 como relleno a 43 pacientes que presentaron pérdida de tejido óseo; se utilizó el biomaterial como sustituto del injerto óseo autólogo o de hueso liofilizado. Se empleó la clasificación y algoritmo descrito por Putnam para valorar la conducta a seguir con los pacientes. Los tipos de fractura más frecuentes fueron la C2, C3 y A3 con 20, 13 y 9 casos respectivamente. Se utilizó el método evaluativo funcional de Horner, para evaluar a los pacientes desde el punto de vista objetivo y subjetivo.
Following its emergence in late 2019, the spread of SARS-CoV-2.sup.1,2 has been tracked by phylogenetic analysis of viral genome sequences in unprecedented detail.sup.3-5. Although the virus spread ...globally in early 2020 before borders closed, intercontinental travel has since been greatly reduced. However, travel within Europe resumed in the summer of 2020. Here we report on a SARS-CoV-2 variant, 20E (EU1), that was identified in Spain in early summer 2020 and subsequently spread across Europe. We find no evidence that this variant has increased transmissibility, but instead demonstrate how rising incidence in Spain, resumption of travel, and lack of effective screening and containment may explain the variant's success. Despite travel restrictions, we estimate that 20E (EU1) was introduced hundreds of times to European countries by summertime travellers, which is likely to have undermined local efforts to minimize infection with SARS-CoV-2. Our results illustrate how a variant can rapidly become dominant even in the absence of a substantial transmission advantage in favourable epidemiological settings. Genomic surveillance is critical for understanding how travel can affect transmission of SARS-CoV-2, and thus for informing future containment strategies as travel resumes.
GAMMA RAY BURST FOLLOW-UPS WITH BOOTES Sergey Guziy; Alberto J. Castro-Tirado; Martin Jelínek ...
Odessa astronomical publications,
10/2014, Letnik:
27, Številka:
1
Journal Article
Recenzirano
Odprti dostop
The Burst Observer and Optical Transient Exploring System (BOOTES), is a global robotic observatory network, which started in 1998 with Spanish leadership devoted to study optical emissions from ...gamma ray bursts (GRBs) that occur in the Universe. We present shot history and current status of BOOTES-4 telescope. Some details of 38 GRBs followed-up with BOOTES-4 are discussed.
Safflower cultivation is of great socioeconomic importance worldwide. Production is intended for the extraction of oil from the seeds. In 2021 Mexico ranked fifth in world production with ...approximately 52,553.28 tons (SIAP, 2021). In April 2022, in the north-central zone of Sinaloa, Mexico, diseased plants were reported in fields planted with safflower. Symptoms included chlorotic plants, necrosis and rot in vascular bundles, dwarfed plants and reflexed plants bent towards the ground. The disease caused estimated losses of 15% of seed production, with respect to the production obtained from the previous year in the safflower fields surveyed. Twenty-five plants with symptoms were sampled to isolate the pathogen. Plants were cut at the base of the stem near the roots and roots cut into 5 mm2 pieces. Tissue samples were superficially disinfected by immersing in 70% alcohol for 10 sec, 2% sodium hypochlorite for 1 min, washed in sterile water, and placed on potato dextrose agar (PDA) at 28 ºC for 7 days in the dark. Twelve monosporic isolates derived from the PDA culture were morphologically characterized. Abundant white aerial mycelium and small pink to dark violet pigments in the center of the culture were observed. From 10-day-old cultures grown on carnation leaf agar medium microconidia and macroconidia were produced. Microconidia were hyaline, had zero to two septa, and were oval or ellipsoidal, 4.6 to 14 x 1.8 to 4.2 μm (n = 40). The macroconidia were hyaline, were slightly curved with three to five septa, and measured from 26 to 69 x 3 to 6.1 μm (n = 40). No chlamydospores were observed. According to the morphological characteristics, the isolates were identified as Fusarium verticillioides (Leslie and Summerell, 2006). DNA was extracted from one isolate and the Translation Elongation Factor 1-α (EF1) gene was amplified and sequenced (O'Donnell et al. 2010). The sequence obtained from isolate FV3CARCULSIN with 645 base pairs was submitted to NCBI GenBank with accession number OQ262963. The BLAST search revealed 100% similarity with F. verticillioides isolate 13 (KM598773) (Lizárraga et al. 2015). Identification in FUSARIUM ID resulted in a 99.85% similarity with isolate F. verticillioides CBS 131389 (MN534047) (Yilmaz et al. 2021). A phylogenetic tree, made with sequences of the EF1 gene, revealed that FV3CARCULSIN was most closely related to F. verticillioides (100% bootstrap). Pathogenicity tests were carried out on safflower plants (cv. Oleico) grown in sterile vermiculite. Plants were inoculated with a conidial suspension (1 × 105 conidia/ml) obtained from FV3CARCULSIN grown on PDA for 7 days. A total of 45 plants were inoculated by drenching the roots with 20 ml of inoculum when the plants were 20 days old. Fifteen plants served as negative controls without inoculation. Plants were kept for 60 days in greenhouse conditions; however, after 45 days the plants began to die. The assay was conducted twice. Rotting and necrosis was observed in the roots of the plants. The pathogen was reisolated from the tissue of all the plants with symptoms and identified as F. verticillioides using morphological characteristics and EF1 sequences, completing Koch's postulates. No symptoms were observed in control plants after 60 days. This is the first report of root rot in safflower caused by F. verticillioides in Mexico. The fungus has been reported in maize (Figueroa et al. 2010), but it is unknown if it could be the same pathogen of safflower. Identification of the pathogen is important for implementing management methods to reduce yield losses and for additional studies on the impact of the disease on oil quality extracted from safflower seeds.
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