As patologias em edificações correspondem a problemas que ocorrem em suas estruturas, tais aspectos podem surgir devido a diversos fatores e agentes causadores, comprometendo a sua segurança e ...durabilidade. Diante disto, a avaliação técnica em projetos é um fator primordial para a identificação de manifestações patológicas e condições estruturais de uma edificação. Analisar tais aspectos é fundamental para garantir maior durabilidade, segurança em toda estrutura e evitar prejuízos maiores. Este estudo teve como objetivo avaliar as condições físicas e estruturais do muro de divisória e instalações dos forros de gesso em duas salas de aulas de uma edificação educacional situada na cidade de Garanhuns-PE. A metodologia aplicada para inspeção foi realizada conforme o enquadramento no Nível 1 (Identificação das anomalias e falhas aparentes, elaborada por profissional habilitado) e de acordo com a norma de Inspeção Predial Nacional do IBAPE. A partir da avaliação estrutural foram identificadas anomalias na extensão do muro, patologias e falhas nos forros de gesso, apresentando um grau de risco considerado como crítico, concluindo-se que é necessário imediatamente uma interdição, demolição e reconstrução.
A matéria orgânica do solo é um relevante controlador da quantidade de fósforo disponível (P) no sistema edáfico. Comparados ao próprio solo, os resíduos orgânicos proporcionam maior disponibilidade ...de fósforo. Acredita-se que o aumento gradativo dos teores de P esteja condicionado principalmente ao material de origem do solo e ao tipo de adubação realizada, onde, a adubação de origem orgânica apresenta teores de três a onze vezes maiores que as de fósforo. Portanto, este estudo buscou avaliar os teores de fósforo disponíveis em áreas de manejo agroecológico em diferentes anos de cultivo, entendendo a influência na manutenção da qualidade do solo. O estudo foi realizado em Governador Dix-Sept Rosado, Rio Grande do Norte, o solo classificado como um Chernossolo Rêndzico. Foram selecionadas parcelas com diferentes tempos de manejo agroecológico e coletadas amostras em duas profundidades: 0-5 e 5-15 cm, correspondente a 1, 6, 8 e 10 anos e uma área de mata nativa como testemunha. Dispondo como referência a mata nativa, os teores máximos de P apresentaram-se de forma crescente ao longo dos 10 anos de manejo agroecológico em ambas as profundidades. Áreas com adição de adubo apresentaram teores de P disponíveis lineares crescentes, ao longo de 10 anos de cultivo.
Objetivo: Analisar o conhecimento e as práticas de usuários com Diabetes Mellitus (DM) acerca da Automonitorização da Glicemia Capilar (AMGC) realizada no domicílio. Métodos: Estudo ...descritivo-exploratório, com metodologia qualitativa, desenvolvido em abril e maio 2013, com usuários com DM atendidos pelo sistema público de saúde de um interior pernambucano. Os dados foram coletados por meio de entrevistas e verificados por análise categorial temática, a amostra foi selecionada por meio da saturação do discurso totalizando 12 usuários com DM que realizam a AMGC no domicílio. Resultados: A análise de conteúdo dos dados possibilitou identificar três categorias: Realização da AMGC de forma não racional; Ausência de programas educativos para orientar sobre a realização da AMGC; Disponibilização insuficiente de insumos aos usuários que realizam a AMGC. Conclusão: Apesar dos avanços legalmente alcançados pelas políticas públicas, os usuários com DM desconhecem a legislação e como está interfere diretamente na realização da AMGC.
Familial hypercholesterolemia (FH) is a common genetic disorder of lipid metabolism caused by pathogenic/likely pathogenic variants in LDLR, APOB, and PCSK9 genes. Variants in FH-phenocopy genes ...(LDLRAP1, APOE, LIPA, ABCG5, and ABCG8), polygenic hypercholesterolemia, and hyperlipoprotein (a) Lp(a) can also mimic a clinical FH phenotype. We aim to present a new diagnostic tool to unravel the genetic background of clinical FH phenotype. Biochemical and genetic study was performed in 1,005 individuals with clinical diagnosis of FH, referred to the Portuguese FH Study. A next-generation sequencing panel, covering eight genes and eight SNPs to determine LDL-C polygenic risk score and LPA genetic score, was validated, and used in this study. FH was genetically confirmed in 417 index cases: 408 heterozygotes and 9 homozygotes. Cascade screening increased the identification to 1,000 FH individuals, including 11 homozygotes. FH-negative individuals (phenotype positive and genotype negative) have Lp(a) >50 mg/dl (30%), high polygenic risk score (16%), other monogenic lipid metabolism disorders (1%), and heterozygous pathogenic variants in FH-phenocopy genes (2%). Heterozygous variants of uncertain significance were identified in primary genes (12%) and phenocopy genes (7%). Overall, 42% of our cohort was genetically confirmed with FH. In the remaining individuals, other causes for high LDL-C were identified in 68%. Hyper-Lp(a) or polygenic hypercholesterolemia may be the cause of the clinical FH phenotype in almost half of FH-negative individuals. A small part has pathogenic variants in ABCG5/ABCG8 in heterozygosity that can cause hypercholesterolemia and should be further investigated. This extended next-generation sequencing panel identifies individuals with FH and FH-phenocopies, allowing to personalize each person’s treatment according to the affected pathway.
The species Inga laurina is native to the Brazilian Cerrado. There are no studies about the chemical composition and biological activities of extracts of this endangered species. The ethanolic ...extract and its successive fractions are rich in phenolic compounds and presented good antifungal activities. HPLC/MS-MS/MS and H1/C13 analysis led to the identification of seventeen compounds, most of which are gallic acid derivatives, myricetin and quercetin glycosides. The ethyl acetate fraction (EAF) contained high levels of total phenolics, expressed in milligrams of gallic acid equivalents per gram of extract (475.3 ± 1.9 mg GAE gextract-1) and flavonoids expressed in milligrams of quercetin equivalents per gram of extract (359.3 ± 10.6 mg QE gextract-1). This fraction was active against fungi of the Candida genus. The EAF showed MIC value 11.7 μg mL−1 against C. glabrata and a selectivity index of 1.6 against Vero cells. The flavonol glycoside myricetin-3-O-rhamnoside was isolated for the first time from the Inga laurina. These results make I. laurina a promising plant as a source of pharmaceutical and biological active antifungal compounds.
ABSTRACT The phytonematodes seriously harm plants worldwide, reducing agricultural productivity, especially root-knot nematodes, genus Meloidogyne. An alternative form of control for this pathogen is ...the use of essential oils, which have a complex mixture of compounds with potential nematicidal activity. The objective of this work was to identify the chemical compounds present in the essential oil of pequi and to verify the efficiency of the oil in the control of Meloidogyne javanica. The identification of chemical compounds was performed by comparing the mass spectra obtained with the spectra present in the equipment library, Nist08. Eighty-second stage juveniles (J2) were incubated in the following treatments: control 0 (2 mL of distilled water) 2, 4, 8 and 16 mg L-1 of pequi essential oil (PEO). The results obtained in the experiment were significant, with a high percentage of mortality of J2 in the treatment T5 (16 mg L-1) of 82% and T3 (4 mg L-1) 66%, respectively. Among the compounds identified, 58.3% are esters, highlight for ethyl hexanoate, and 25.0% are monoterpenes, and all are nematicidal compounds. Pequi essential oil has the potential to be used in the control of M. javanica.
Anadenanthera colubrina, popularly known as white angico, is a species extensively cultivated in Brazil, mainly in the cerrado region, including the state of Piauí. This study examines the ...development of films composed of white angico gum (WAG) and chitosan (CHI) and containing chlorhexidine (CHX), an antimicrobial agent. The solvent casting method was used to prepare films. Different combinations and concentrations of WAG and CHI were used to obtain films with good physicochemical characteristics. Properties such as the in vitro swelling ratio, the disintegration time, folding endurance, and the drug content were determined. The selected formulations were characterised by scanning electron microscopy, Fourier-transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, and X-ray diffraction, and the CHX release time and antimicrobial activity were evaluated. CHX showed a homogenous distribution in all CHI/WAG film formulations. The optimised films showed good physicochemical properties with 80% CHX release over 26 h, which is considered promising for local treatment of severe lesions in the mouth. Cytotoxicity tests of the films did not show toxicity. The antimicrobial and antifungal effects were very effective against the tested microorganisms.
Triatoma dimidiata, a Chagas disease vector widely distributed along Central America, has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in ...peridomestic areas fed human blood. Exploring the salivary compounds that overcome host haemostatic and immune responses is of great scientific interest. Here, we provide a deeper insight into its salivary gland molecules. We used high-throughput RNA sequencing to examine in depth the T. dimidiata salivary gland transcriptome. From >51 million reads assembled, 92.21% are related to putative secreted proteins. Lipocalin is the most abundant gene family, confirming it is an expanded family in Triatoma genus salivary repertoire. Other putatively secreted members include phosphatases, odorant binding protein, hemolysin, proteases, protease inhibitors, antigen-5 and antimicrobial peptides. This work expands the previous set of functionally annotated sequences from T. dimidiata salivary glands available in NCBI from 388 to 3815. Additionally, we complemented the salivary analysis through proteomics (available data via ProteomeXchange with identifier PXD008510), disclosing the set complexity of 119 secreted proteins and validating the transcriptomic results. Our large-scale approach enriches the pharmacologically active molecules database and improves our knowledge about the complexity of salivary compounds from haematophagous vectors and their biological interactions.
Several haematophagous triatomine species can transmit Trypanosoma cruzi, the etiological agent of Chagas disease. Due to the reemergence of this disease, new drugs for its prevention and treatment are considered priorities. For this reason, the knowledge of vector saliva emerges as relevant biological finding, contributing to the design of different strategies for vector control and disease transmission. Here we report the transcriptomic and proteomic compositions of the salivary glands (sialome) of the reduviid bug Triatoma dimidiata, a relevant Chagas disease vector in Central America. Our results are robust and disclosed unprecedented insights into the notable diversity of its salivary glands content, revealing relevant anti-haemostatic salivary gene families. Our work expands almost ten times the previous set of functionally annotated sequences from T. dimidiata salivary glands available in NCBI. Moreover, using an integrated transcriptomic and proteomic approach, we showed a correlation pattern of transcription and translation processes for the main gene families found, an important contribution to the research of triatomine sialomes. Furthermore, data generated here reinforces the secreted proteins encountered can greatly contribute for haematophagic habit, Trypanosoma cruzi transmission and development of therapeutic agent studies.
Display omitted
•We provide a Triatoma dimidiata sialome analysis.•We were able to extract 3,815 CDS from transcriptomic analysis.•119 salivary proteins were identified by proteomic analysis.•The results disclosed relevant salivary molecules to the vector-host interaction for Triatoma dimidiata species.
Sugarcane Harvesting Quality by Digital Image Processing De Moura Araújo, Guilherme; dos Santos, Fernando Ferreira Lima; de Almeida, Samira Luns Hatum ...
Sugar tech : an international journal of sugar crops & related industries,
02/2021, Letnik:
23, Številka:
1
Journal Article
Recenzirano
Sugarcane, a major crop in the Brazilian agribusiness sector, has had significant changes in its cultivation over the years. The harvest of sugarcane was usually carried out manually. However, due to ...the great demand for labours and high health hazards, manual harvesting was replaced by a mechanized process. Harvesters have a basal cutting mechanism composed of blades that are responsible for cutting the sugarcane. The blades must be continuously analysed and replaced if necessary. The analysis of these blades is performed qualitatively, in which the operator analyses the current conditions of the blades (based on prior experiences); or quantitatively, using a digital calliper to measure the thickness of the blade, wherein the increase in thickness indicates greater wear. Both approaches require the harvester to be stationary and are extremely time-consuming. In order to obtain more efficient results, the present study proposes a novel method to analyse blade wear and harvesting quality through the use of digital image processing. The change in geometric characteristics (area, perimeter, rectangularity, and length) of the blades over time was evaluated along with blade wear and harvesting quality indices (damage index to stalks). The obtained results indicated that the proposed methodology was effective at assessing the quality of the harvesting operation of sugarcane and the wear in the mechanisms of basal cutting present in sugarcane harvesters. The error in estimating the damage index ranged between 0.030 and 0.033%.
The elderly is a risk group for changes in health conditions and as well as in functional performance related to the decline in grip strength and reduced walking speed, which can compromise the ...performance of activities of daily living. Based on this, the objective was to analyze the factors associated with functional performance in the elderly. For that, a cross-sectional study was developed involving a group of 179 elderly people who received medical care at an outpatient clinic of the public health network. Muscle strength and gait speed were measured. Nutritional status was determined by Body Mass Index (BMI), calf circumference (CC), waist circumference (WC) and waist-to-hip ratio (WHR). Level of physical activity was measured by the adapted version of the Minnesota Leisure Activities Questionnaire. Low muscle strength was found in 20.7% of the elderly and slow gait in 20.1%. Increased risk of cardiovascular disease was found in 40.8% considering WC, and 35.2% by WHR. Most of the elderly had low weight and no loss of muscle mass. Loss of muscle strength was associated with sex and CC and gait speed were associated with sex, age, and presence of a partner (p < 0.05). The chance of loss of muscle strength and lower gait speed was higher in elderly women. Loss of muscle mass due to CC was associated with the chance of loss of strength, and the chance of reduced walking speed was greater among the elderly individuals aged 75 years and over.
PDF
