Supplementing minerals beyond dietary requirements can increase the risk of toxicity and mineral excretion, making the selection of more bioavailable sources crucial. Thus, this work aimed to use ...metalloproteomics tools to investigate possible alterations in the hepatic proteome of broilers fed with diets containing two sources (sulfate and hydroxychloride) and two levels of copper (15 and 150 ppm) and manganese (80 and 120 ppm), totaling four treatments: low Cu/Mn SO
, high Cu/Mn SO
, low Cu/Mn (OH)Cl and high Cu/Mn (OH)Cl. The difference in abundance of protein spots and copper and manganese concentrations in liver and protein pellets were analyzed by analysis of variance with significance level of 5%. The Cu and Mn concentrations determined in liver and protein pellets suggested greater bioavailability of hydroxychloride sources. We identified 19 Cu-associated proteins spots, 10 Mn-associated protein spots, and 5 Cu and/or Mn-associated protein spots simultaneously. The analysis also indicated the induction of heat shock proteins and detoxification proteins in broilers fed with high levels of copper and manganese, suggesting the involvement of these proteins in metal tolerance and stress.
Monilinia fructicola (Wint.) Honey is a plant pathogenic fungus that infects stone fruits such as peach, nectarine and plum, which are high demand cultivars found in Brazil. This pathogen may remain ...latent in the host, showing no apparent signs of disease, and consequently may spread to different countries. The aim of this study was to evaluate the activity of hydroalcoholic extract (HydE) obtained from Lactarius deliciosus (L.) Sf. Gray a mushroom, against M. fructicola phytopathogenic-induced mycelial growth. In addition, the purpose of this study was to examine phytotoxicity attributed to HydE using Brassica oleracea seeds, as well as cytotoxic analysis of this extract on cells of mouse BALB/c monocyte macrophage cell line (J774A.1 cell line) (ATCC TIB-67). The L. deliciosus HydE inhibited fungal growth and reduced phytopathogen mycelial development at a concentration of 1.25 mg/ml. Our results demonstrated that the extract exhibited phytotoxicity as evidenced by (1) interference on germination percentage and rate index, (2) decreased root and initial growth measures, and (3) lower fresh weight of seedlings but no cytotoxicity in Vero cell lines. Data suggest that the use of the L. deliciosus extracts may be beneficial for fungal control without any apparent adverse actions on mouse BALB/c monocyte macrophage cell line (J774A.1 cell line) viability.
Bioelectrical impedance vector analysis (BIVA) is able to identify differences in hydration status and body composition components, such as body cell mass (BCM) by means of plotting individuals in ...ellipses, when comparing groups with different characteristics.
Compare the confidence and tolerance ellipses of BIVA in individual and team sports athletes with a non-athlete reference population.
One hundred sixty-seven college athletes (team sports: 117 athletes, individual sports: 50 athletes) aged between 18 and 35 years. Bioelectrical impedance was used to measure resistance (R) and reactance (Xc) values that were used in the BIVA analysis to identify hydration status and BCM, respectively. Hotelling's t-test was used to identify differences between groups in the confidence ellipses and the comparison was made with a non-athlete Italian reference population.
There were no differences between male team sports and individual athletes (p = 0.151) and for female athletes (p = 0.624). Most athletes were located in the 50% tolerance ellipses, indicating adequate hydration. Compared to the Italian a non-athlete population, athletes of both sexes presented left impedance vector deviation in the minor axis (Xc) of the tolerance ellipses, indicating higher BCM.
There were no differences in BIVA between team and individual sports athletes, but most athletes presented an adequate hydration state and, compared to a non-athlete population, the athletes of the present study presented higher BCM.
Objective: To compare indicators of fluid distribution and cellular integrity in university athletes through a cross-sectional study of 167 university athletes (18-35 years) who competed in team ...sports and individual sports. Approach: Bioimpedance was used to estimate total body water (TBW), intracellular water (ICW), extracellular water (ECW), ECW/ICW ratio, impedance (Z), reactance (Xc), resistance (R), phase angle, body cell mass (BCM) and ECW/BCM ratio at a frequency of 50 kHz. X-ray absorptiometry was used to determine lean soft tissue mass and body fat. As covariables, sex, age, time of practice and type of modality were obtained by questionnaire. Main results: Individual male athletes presented higher values of ECW/ICW ratio (means difference MD = 0.006, p = 0.020) and ECW/BCM ratio (MD = 0.006, p = 0.017) than team sports male athletes. Values of Z (MD = 24.988, p = 0.009), Xc (MD = 3.327, p = 0.008) and R (MD = 24.755, p = 0.09) were higher in male athletes practicing team sports than individual sports. Female team sports athletes presented higher values of Z (MD = 32.029, p = 0.015) and R (MD = 31.987, p = 0.015) than individual female athletes. It was concluded that ECW/ICW and ECW/BCM ratios were higher in male athletes who practiced individual sports. For the Z, R, and Xc values, the athletes in team sports presented higher values. The female athletes who practiced team sports presented higher values of Z and R than did individual female athletes. Significance: ECW/ICW and ECW/BCM ratios were higher in male athletes who practiced individual sports. For the Z, R, and Xc values, the athletes in team sports presented higher values. The female athletes who practiced team sports presented higher values of Z and R than did individual female athletes.
Maropitant, an antagonist of neurokinin-1 (NK-1) receptors, blocks the pharmacological action of substance P on the central and peripheral nervous systems. The objective of this study was to compare ...the antinociceptive and cardiorespiratory effects of the continuous intraoperative infusion of maropitant with ketamine and lidocaine in female dogs undergoing unilateral radical mastectomy. Twenty-four female dogs were used and were divided randomly into two groups (n = 12). The GLK group received ketamine bolus (1.0 mg/kg), lidocaine bolus (1.5 mg/kg), and continuous infusion of ketamine and lidocaine (10 mcg/kg/min and 50 mcg/kg/min), respectively; the GLKM group received the same anesthetic protocol combined with maropitant bolus (1.5 mg/kg/IV) and continuous infusion of maropitant (100 mcg/kg/h). Continuous infusion was initiated at the start of surgery and was maintained until 1 hour postoperatively. Pain was evaluated in the postoperative period using four scales and a digital analgesimeter. Data were analysed using analysis of variance, Student's t-test, Mann–Whitney test, and Friedman’s test (P < 0.05). Kaplan–Meier curves were compared using the log-rank test. The results indicated lower pain scores, better survival curves with a lower number of patients requiring rescue analgesia, and lower peripheral sensitization, in the GLKM group than in the GLK group. It was concluded that the coadministration of maropitant with ketamine and lidocaine had an adjuvant effect with minimal cardiorespiratory effects and effective analgesia, improving pain management and patient comfort.
Information on natural resource exploitation is vital for conservation but scarce in developing nations, which encompass most of the world and often lack the capacity to produce it. A growing ...approach to generate information about resource use in the context of developing nations relies on surveys of resource users about their recollections (recall) of past harvests. However, the reliability of harvest recalls remains unclear. Here, we show that harvest recalls can be as accurate to data collected by standardized protocols, despite that recalls are variable and affected by the age of the recollecting person and the length of time elapsed since the event. Samples of harvest recalls permit relatively reliable reconstruction of harvests for up to 39 years in the past. Harvest recalls therefore have strong potential to inform data‐poor resource systems and curb shifting baselines around the world at a fraction of the cost of conventional approaches.
The bioactive compounds produced by Streptomyces spp. bacteria can inhibit and reduce the incidence of phytopathogenic fungi in agricultural crops. In this study, two Streptomyces strains 6(4) and ...R18(6) were tested separately and in a consortium and demonstrated antifungal activity against the phytopathogenic fungus Pyrenophora tritici-repentis (Ptr) that causes tan spots in wheat plants. Streptomyces isolates inhibited mycelial growth and they induce changes in hyphal development in the germination of Ptr. In the dual culture assay, the Streptomyces 6(4) strain inhibited growth of Ptr by 44% and in the agar well diffusion assay, produced the largest inhibition halos when the crude extract was tested (≥20 mm). The detached leaf assay showed that Streptomyces 6(4) and R18(6) strains and consortium crude extracts, reduced the disease index on the leaves of wheat, with 100, 90, and 95% biocontrol efficacy, respectively. Inoculating seeds with the propagules of spores of the same strains, 6(4), R18(6) and consortium, also resulted in biocontrol of the pathogen on detached leaves, although with 48, 53.08 and 60% biocontrol efficacy respectively. Therefore, we can conclude that the crude extracts of these isolates have the potential to control the fungus Pyrenophora tritici-repentis and reduce the symptoms of tan spot in wheat.
Laccases produced by
Leucoagaricus gongylophorus
act in lignocellulose degradation and detoxification processes. Therefore, the use of
L. gongylophorus
laccase (Lac1Lg) was proposed in this work for ...degradation of anthracene and others polycyclic aromatic hydrocarbons without the use of mediators. Degradation reactions were performed in buffer aqueous solution with 10 ppm of anthracene and other PAHs, Tween-20 in 0.25%
v
/
v
and a laccase preparation of 50 U. The optimum condition (pH 6.0 and 30 °C) was determined by response surface methodology with an excellent coefficient of determination (
R
2
) of 0.97 and an adjusted coefficient of determination (
R
2
adj
) of 0.93. In addition, the employment of the mediator ABTS decreased the anthracene biodegradation from 44 ± 1% to 30 ± 1%. This optimum pH of 6.0 suggests that the reaction occurs by a hydrogen atom transfer mechanism. Additionally, in 24 h Lac1Lg biodegraded 72 ± 1% anthracene, 40 ± 3% fluorene and 25 ± 3% phenanthrene. The yellow laccase from
L. gongylophorus
biodegraded anthracene and produced anthrone and anthraquinone, which are interesting compounds for industrial applications. Moreover, this enzyme also biodegraded the PAHs phenanthrene and fluorene justifying the study of Lac1Lg for bioremediation of these compounds in the environment.
Carbapenemase-producing Klebsiella pneumoniae has become a global priority, not least in low- and middle-income countries. Here, we report the emergence and clinical impact of a novel Klebsiella ...pneumoniae carbapenemase-producing K. pneumoniae (KPC-KP) sequence type (ST) 16 clone in a clonal complex (CC) 258-endemic setting.
In a teaching Brazilian hospital, a retrospective cohort of adult KPC-KP bloodstream infection (BSI) cases (January 2014 to December 2016) was established to study the molecular epidemiology and its impact on outcome (30-day all-cause mortality). KPC-KP isolates underwent multilocus sequence typing. Survival analysis between ST/CC groups and risk factors for fatal outcome (logistic regression) were evaluated. Representative isolates underwent whole-genome sequencing and had their virulence tested in a Galleria larvae model.
One hundred sixty-five unique KPC-KP BSI cases were identified. CC258 was predominant (66%), followed by ST16 (12%). The overall 30-day mortality rate was 60%; in contrast, 95% of ST16 cases were fatal. Patients' severity scores were high and baseline clinical variables were not statistically different across STs. In multivariate analysis, ST16 (odds ratio OR, 21.4; 95% confidence interval CI, 2.3-202.8; P = .008) and septic shock (OR, 11.9; 95% CI, 4.2-34.1; P < .001) were independent risk factors for fatal outcome. The ST16 clone carried up to 14 resistance genes, including blaKPC-2 in an IncFIBpQIL plasmid, KL51 capsule, and yersiniabactin virulence determinants. The ST16 clone was highly pathogenic in the larvae model.
Mortality rates were high in this KPC-KP BSI cohort, where CC258 is endemic. An emerging ST16 clone was associated with high mortality. Our results suggest that even in endemic settings, highly virulent clones can rapidly emerge demanding constant monitoring.
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