•Lipid production and growth by R. glutinis R4 and other oleaginous yeasts were compared.•R. glutinis R4 showed remarkable capacity for lipid production and fast growth.•Microbial oils produced by R. ...glutinis R4 are similar to vegetable oils.•Fatty Acyl Methyl Esters (FAME) produced from R. glutinis R4 lipids are adequate for biodiesel synthesis.•Biodiesel derived from R. glutinis R4 complies with international fuel standards.
Some Rhodotorula spp. have been characterized as oleaginous yeasts. Under certain culture conditions they can accumulate neutral lipids, which are mainly triglycerides (TAG). Microbial TAG that can be used as raw material for biodiesel synthesis are attractive for the biofuel industry. In this study, the ability to synthesize lipids of Rhodotorula glutinis R4, isolated in Antarctica, was compared with eight strains belonging to the genera Rhodotorula and Yarrowia with the aim of proposing a novel source of oils for biodiesel synthesis. All strains were cultured under nitrogen (N) limiting conditions and an excess of carbon (C) in the culture medium. We found that yeasts accumulated between 9–48.9 % (w/w) of lipids. Among them, R. glutinis R4 showed the highest growth (14 g L−1, μmax 0,092 h−1) and lipid production (7 g L−1; 47 % w/w). Microbial oils produced by R. glutinis R4 are similar to vegetable oils, with 61 % of oleic acid, indicating that it is adequate for biodiesel synthesis. Our results demonstrate that biodiesel derived from R. glutinis R4 complies with international fuel standards ASTM D6751 and EN 14214. Therefore, this work demonstrates that Rhodotorula glutinis R4 is a novel and valuable source of microbial oils for biodiesel synthesis.
The placement of obstacles in front of doors is believed to be an effective strategy to increase the flow of pedestrians, hence improving the evacuation process. Since it was first suggested, this ...counterintuitive feature is considered a hallmark of pedestrian flows through bottlenecks. Indeed, despite the little experimental evidence, the placement of an obstacle has been hailed as the panacea for solving evacuation problems. In this work, we challenge this idea and experimentally demonstrate that the pedestrians flow rate is not necessarily altered by the presence of an obstacle. This result-which is at odds with recent demonstrations on its suitability for the cases of granular media, sheep and mice-differs from the outcomes of most of existing numerical models, and warns about the risks of carelessly extrapolating animal behaviour to humans. Our experimental findings also reveal an unnoticed phenomenon in relation with the crowd movement in front of the exit: in competitive evacuations, an obstacle attenuates the development of collective transversal rushes, which are hazardous as they might cause falls.
Several theoretical predictions of the mass flow rate of granular media discharged from a silo are based on the spontaneous development of a free-fall arch region, the existence of which is still ...controversial. In this Letter, we study experimentally and numerically the particle flow through an orifice placed at the bottom of 2D and 3D silos. The implementation of a coarse-grained technique allows a thorough description of all the kinetic and micromechanical properties of the particle flow in the outlet proximities. Though the free-fall arch does not exist as traditionally understood--a region above which particles have negligible velocity and below which particles fall solely under gravity action--we discover that the kinetic pressure displays a well-defined transition in a position that scales with the outlet size. This universal scaling explains why the free-fall arch picture has served as an approximation to describe the flow rate in the discharge of silos.
Based on the implementation of a novel silo discharge procedure, we are able to control the grains velocities regardless of the outlet size. This allows isolating the geometrical and kinematic ...contributions to the clogging process. We find that, for a given outlet size, reducing the grains velocities to extremely low values leads to a clogging probability increment of almost two orders of magnitude, hence revealing the importance of particle kinematics in the silo clogging process. Then, we explore the contribution of both variables, outlet size and grains velocity, and we find that our results agree with an already known exponential expression that relates clogging probability with outlet size. We propose a modification of such expression revealing that only two parameters are necessary to fit all the data: one is related with the geometry of the problem, and the other with the grains kinematics.
The dynamics of granular media within a silo in which the grain velocities are controlled by a conveyor belt has been experimentally investigated. To this end, the building of coarse-grained field ...maps of different magnitudes has allowed a deep analysis of the flow properties as a function of two parameters: the orifice size and the belt velocity. First, the internal dynamics of the particles within the silo has been fully characterized by the solid fraction, the velocity of the particles and the kinetic stress. Then, the analysis of the vertical profiles of the same magnitude (plus the acceleration) has allowed connection of the internal dynamics with the flow rate. In particular, we show that the gamma parameter – which accounts for the integration of the normalized acceleration along the vertical direction – can successfully discriminate the kind of flow established within the silo (from the quasistatic regime to the free discharge) depending on the outlet size and belt velocity.
We experimentally analyze the compaction dynamics of an ensemble of cubic particles submitted to a novel type of excitation. Instead of the standard tapping procedure used in granular materials we ...apply alternative twists to the cylindrical container. Under this agitation, the development of shear forces among the different layers of cubes leads to particle alignment. As a result, the packing fraction grows monotonically with the number of twists. If the intensity of the excitations is sufficiently large, an ordered final state is reached where the volume fraction is the densest possible compatible with the boundary condition. This ordered final state resembles the tetratic or cubatic phases observed in colloids.
Cyclin-dependent kinase 12 (CDK12) aberrations have been reported as a biomarker of response to immunotherapy for metastatic castration-resistant prostate cancer (mCRPC). Herein, we characterize ...CDK12-mutated mCRPC, presenting clinical, genomic, and tumor-infiltrating lymphocyte (TIL) data.
Patients with mCRPC consented to the molecular analyses of diagnostic and mCRPC biopsies. Genomic analyses involved targeted next-generation (MiSeq; Illumina) and exome sequencing (NovaSeq; Illumina). TILs were assessed by validated immunocytochemistry coupled with deep learning-based artificial intelligence analyses including multiplex immunofluorescence assays for CD4, CD8, and FOXP3 evaluating TIL subsets. The control group comprised a randomly selected mCRPC cohort with sequencing and clinical data available.
Biopsies from 913 patients underwent targeted sequencing between February 2015 and October 2019. Forty-three patients (4.7%) had tumors with CDK12 alterations. CDK12-altered cancers had distinctive features, with some revealing high chromosomal break numbers in exome sequencing. Biallelic CDK12-aberrant mCRPCs had shorter overall survival from diagnosis than controls 5.1 years (95% confidence interval (CI), 4.0-7.9) vs. 6.4 years (95% CI, 5.7-7.8); hazard ratio (HR), 1.65 (95% CI, 1.07-2.53);
= 0.02. Median intratumoral CD3
cell density was higher in CDK12 cancers, although this was not statistically significant (203.7 vs. 86.7 cells/mm
;
= 0.07). This infiltrate primarily comprised of CD4
FOXP3
cells (50.5 vs. 6.2 cells/mm
;
< 0.0001), where high counts tended to be associated with worse survival from diagnosis (HR, 1.64; 95% CI, 0.95-2.84;
= 0.077) in the overall population.
CDK12-altered mCRPCs have worse prognosis, with these tumors surprisingly being primarily enriched for CD4
FOXP3
cells that seem to associate with worse outcome and may be immunosuppressive.
.
Hypoxia is defined as a reduction in oxygen supply to a tissue below physiological levels. However, physiological hypoxic conditions occur during early embryonic development; and in adult organisms, ...many cells such as bone marrow stem cells are located within hypoxic niches. Thus, certain processes take place in hypoxia, and recent studies highlight the relevance of hypoxia in stem cell cancer physiology. Cellular response to hypoxia depends on hypoxia-inducible factors (HIFs), which are stabilized under low oxygen conditions. In a hypoxic context, various inducible HIF alpha subunits are able to form dimers with constant beta subunits and bind the hypoxia response elements (HRE) in the genome, acting as transcription factors, inducing a wide variety of gene expression. Typically, the HIF pathway has been shown to enhance vascular endothelial growth factor (VEGF) expression, which would be responsible for angiogenesis and, therefore, re-oxygenation of the hypoxic sites. Embryonic stem cells inhibit a severely hypoxic environment, which dictates their glycolytic metabolism, whereas differentiated cells shift toward the more efficient aerobic respiration for their metabolic demands. Accordingly, low oxygen tension levels have been reported to enhance induced pluripotent stem cell (iPS) generation. HIFs have also been shown to enhance pluripotency-related gene expression, including Oct4 (Octamer-binding transcription factor 4), Nanog and Wnt. Therefore, cell metabolism might play a role in stemness maintenance, proliferation and cell reprogramming. Moreover, in the hypoxic microenvironment of cancer cells, metabolism shifts from oxidative phosphorylation to anaerobic glycolysis, a process known as the Warburg effect, which is involved in cancer progression and malignancy.
•Correlate Electrical Resistivity Tomography with metallic concentration data.•Modelling spatial distribution of heavy metal concentrations statistically.•Assessment of a forsaken phosphogypsum pond ...efficiently.
One of the wastes generated in fertiliser production from phosphoric rock is phosphogypsum, whose mismanagement lead to environmental and health risks. Therefore, a detailed evaluation of the chemical composition of phosphogypsum is necessary to determine effective means of its management. Due to the high amount of generated waste, the cost and time consumed for this characterisation by chemical analysis is limiting. Hence, efficient tools should be developed to predict the chemical composition of this waste. Thus, this study aims to: 1) determine the physic-chemical characterisation of phosphogypsum pond using geochemical and geophysical techniques and 2) predict the heavy metals spatial distribution through statistical models. Results show that the most concentrate metal is chromium with a maximum of ≈900 mg.kg−1 and cadmium is the least concentrated (maximum ≈23 mg.kg-1). The Electrical Resistivity Tomography revealed the superposition of two layers. The top one (waste) presents low resistivity (≈17Ω.m) while the bottom layer shows higher resistivity (>124Ω.m). Metal concentrations and resistivities were combined by applying non-linear regression models. Cr showed the strongest correlation (R2 = 0.68), yielding an accurate model that was used for revealing the spatial distribution of the highest Cr concentrations in the pond, with the consequent reduction of expensive traditional methods.
The lack of environmental regulations before 1998 brought about significant ecological consequences in Europe. There are 4,000,000 ha potentially contaminated by waste, resulting from industrial ...activity. These sites present abnormal values of salinity, alkalinity, and organic and inorganic pollutants. A representative example of this is the production of fertiliser derived from phosphoric rock through the wet acid process which mainly produces phosphogypsum and pyrite ashes. For each tonne of fertiliser produced, five tonnes of phosphogypsum are generated, which in most of the cases were simply piled into non-conditionate deposits; currently, there is no information about these deposits. Hence, fast and affordable methodologies should be developed to calculate the volume contained in the existing waste deposits. Thus, this study aims to: i) scan the industrial area selected in order to identify the distribution and possible variants of the waste that make up the configuration of geoelectrical profiles and boreholes, and ii) accurately determine the volume contained in the chosen deposits by merging Electrical Resistivity Tomography with LiDAR point cloud. Results show a range of deposit volumes that run from 4900 m3 to 59,300 m3. The density of LiDAR point cloud (1 point/m2) ensures metric precision for the superficial layer. The Electrical Resistivity Tomography revealed the geometry of the deposits that superposes two layers marking the border between waste and natural terrain, and the boreholes guarantee the exactness of the waste layer thickness. This synergistic combination of Electrical Resistivity Tomography with LiDAR point cloud yields an accurate method that we used to calculate the volume of waste present in the deposits.
Display omitted
•Correlation of borehole data with Electrical Resistivity Tomography profiles•Topographic layer modelling based on LiDAR point cloud•Volumetric assessment of forsaken waste deposits
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