Here we determined the impact of salt shock and salt stress on the level of DNA methylation in selected CpG islands localized in promoters or first exons of sixteen salt-responsive genes in beets. ...Two subspecies differing in salt tolerance were subjected for analysis, a moderately salt-tolerant sugar beet Beta vulgaris ssp. vulgaris cv. Huzar and a halophytic beet, Beta vulgaris ssp. maritima. The CpG island methylation status was determined. All target sequences were hyper- or hypomethylated under salt shock and/or salt stress in one or both beet subspecies. It was revealed that the genomic regions analyzed were highly methylated in both, the salt treated plants and untreated controls. Methylation of the target sequences changed in a salt-dependent manner, being affected by either one or both treatments. Under both shock and stress, the hypomethylation was a predominant response in sugar beet. In Beta vulgaris ssp. maritima, the hypermethylation occurred with higher frequency than hypomethylation, especially under salt stress and in the promoter-located CpG sites. Conversely, the hypomethylation of the promoter-located CpG sites predominated in sugar beet plants subjected to salt stress. This findings suggest that DNA methylation may be involved in salt-tolerance and transcriptomic response to salinity in beets.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Ascorbate oxidase, which is known to play a key role in regulating the redox state in the apoplast, cell wall metabolism, cell expansion and abiotic stress response in plants, oxidizes apo-plastic ...ascorbic acid (AA) to dehydroascorbic acid (DHA). However, there is little information about the AAO genes and their functions in beets under abiotic stress. The term salt or drought stress refers to the treatment of plants with slow and gradual salinity/drought. Contrastingly, salt shock consists of exposing plants to high salt levels instantaneously and drought shock occurs under fast drought progression. In the present work, we have subjected plants to salinity or drought treatments to elicit either stress or shock and carried out a genome-wide analysis of ascorbate oxidase (AAO) genes in sugar beet (B. vulgaris cv. Huzar) and its halophytic ancestor (B. maritima). Here, conserved domain analyses showed the existence of twelve BvAAO gene family members in the genome of sugar beet. The BvAAO_1–12 genes are located on chromosomes 4, 5, 6, 8 and 9. The phylogenetic tree exhibited the close relationships between BvAAO_1-12 and AAO genes of Spinacia oleracea and Chenopodium quinoa. In both beet genotypes, downregulation of AAO gene expression with the duration of salt stress or drought treatment was observed. This correlated with a decrease in AAO enzyme activity under defined experimental setup. Under salinity, the key downregulated gene was BvAAO_10 in Beta maritima and under drought the BvAAO_3 gene in both beets. This phenomenon may be involved in determining the high tolerance of beet to salinity and drought.
Contamination of vegetables with human pathogenic microorganisms (HPMOs) is considered one of the most important problems in the food industry, as current nutritional guidelines include increased ...consumption of raw or minimally processed organic vegetables due to healthy lifestyle promotion. Vegetables are known to be potential vehicles for HPMOs and sources of disease outbreaks. In this study, we tested the susceptibility of radish (
) to colonization by different HPMOs, including
PCM 2561,
subsp.
PCM 2565,
PCM 2191 and
PCM 1948. We hypothesized that host plant roots containing bactericidal compounds are less prone to HPMO colonization than shoots and leaves. We also determined the effect of selected pathogens on radish growth to check host plant-microbe interactions. We found that one-week-old radish is susceptible to colonization by selected HPMOs, as the presence of the tested HPMOs was demonstrated in all organs of
. The differences were noticed 2 weeks after inoculation because
was most abundant in roots (log
CFU - 2.54),
was observed exclusively in stems (log
CFU - 3.15), and
and
were most abundant in leaves (log
CFU - 4.80 and 3.23, respectively). The results suggest that
and
show a higher ability to colonize and move across the plant than
and
. Based on fluorescence
hybridization (FISH) and confocal laser scanning microscopy (CLSM) approach HPMOs were detected in extracellular matrix and in some individual cells of all analyzed organs. The presence of pathogens adversely affected the growth parameters of one-week-old
especially leaf and stem fresh weight (decreased by 47-66 and 17-57%, respectively). In two-week-old plants, no reduction in plant biomass development was noted. This observation may result from plant adaptation to biotic stress caused by the presence of HPMOs, but confirmation of this assumption is needed. Among the investigated HPMOs,
turned out to be the pathogen that most intensively colonized the aboveground part of
and at the same time negatively affected the largest number of radish growth parameters.
Climate change and warming affect the development cycle of ornamental plants and, thus, their quality and ornamental value. The study aimed to investigate the flowering phenology of 37 cultivars of ...shrub roses in response to meteorological conditions based on eight years of observations. Based on 14,800 field records, we grouped shrub rose cultivars according to their dominant flowering stage as a sensitive indicator of variability in weather conditions. We also selected the rose cultivars that would retain the highest ornamental value even at increasing temperatures. The total flowering duration of the cultivars ranged from 31 to 115 days (mean 90.4 days) and was significantly positively correlated with temperature. Meteorological conditions significantly influenced individual flowering stages (FS) and a trend of changes concerning the long-term gradient was observed: the increase in temperature extended FS10 and shortened FS25, FS50, and FS75 but did not affect FS100. If the warming trend continues, the abundant flowering of roses may be shortened considerably. Rose cultivars with the longest flowering period at FS10 abundance may adapt better to rising temperatures, and, therefore may be more useful in landscape planning and management.
Micropropagation via organogenesis of Lachenalia viridiflora W. F. Barker, a native endangered plant from South Africa, is presented in this study. Leaves, collected from in vivo plants, were a ...source for culture initiation to obtain three types of explants (leaf, bulb, callus). They were grown on Murashige and Skoog (MS) medium supplemented with two concentrations of sucrose (3 or 6%, marked as S3 and S6) and different concentrations of benzylaminopurine (BA) (0.0–5.0 μM) and naphthalene acetic acid (NAA) (0.0–0.5 μM). The results demonstrated that bulbing was more efficient on media with 3% sucrose and this process was closely related to explant type—irrespective of media type twice more adventitious bulbs (5.1–5.6) were regenerated on leaf and bulb explants than on callus (2.6). The highest value of adventitious bulb number was recorded for interaction: leaf × S3/2.5 BA/0.5 NAA (9.3 bulbs per explant). Irrespective of media type, the higher the number of adventitious bulbs (obtained from leaf and bulb explants), the lower their diameter. Moreover, adventitious bulbs regenerated from bulb explants formed the longest leaves (18.9 mm) and in the greatest percentage (52.3%). The highest biomass weight increase indicator (50 times) was recorded for interaction: leaf × S6/2.5 BA/0.25 NAA. Various combinations of plant growth regulators favor biomass increase.
Geophytes are an increasingly widely used group of plants. Its high biodiversity, however, significantly impacts the cultivation process. Therefore, every new insight into the behavioral aspects of ...each single species is crucial for production. This study, for the first time, aims at contributing to a better understanding of the interplay between the bulb size potential of Narcissus poeticus and the pattern of bulb lifting time to find the optimal combination of these factors in obtaining the best quality propagation material. Twelve bulb size groups were examined. Plants were planted in the open air in two locations of Central East Europe and lifted in three terms: immediately and two and four weeks after flowering. Bulb fresh and dry weight, as well as leaf, root and total plant dry weight, increased with growing bulb size and delayed the time of lifting. The bulb weight increase indicator was twice as high for bulbs lifted in the third date compared to those lifted earlier. Its value was the highest for the smallest bulbs. Bulb shape ratio gradually decreased together with the increase of bulb weight and later lifting term. Principal components analysis allowed singling out two principal components that accounted for 86.58% of total variability.
Increasing land salinization in recent decades has led to a decrease in crop productivity worldwide. We hypothesized that bioaugmentation of beetroot (Beta vulgaris) with halotolerant endophytic ...bacterial strains isolated from the obligatory halophytic plant Salicornia europaea L. may mitigate salt stress in new host plants. Therefore, we investigated the effects of inoculation with Pseudomonas stutzeri ISE12 or Kushneria marisflavi CSE9 on B. vulgaris growth in substrates enriched with various NaCl concentrations (0, 50, 150, 300 mM). The results of this study indicated that bioaugmentation with either bacteria resulted in improved growth parameters and increased chlorophyll content, as well as decreased proline and hydrogen peroxide concentrations, in B. vulgaris organs. However, K. marisflavi CSE9 was more efficient in achieving salt stress mitigation than P. stutzeri ISE12. In conclusion, the range of salinity tolerance seems to be a key parameter in the selection of strains for beet inoculation. The selected halotolerant endophytes (P. stutzeri ISE12 and K. marisflavi CSE9) isolated from the roots of obligatory halophytic S. europaea may be employed for plant growth promotion, especially in saline areas, and have potential applications in sustainable agriculture.
The Narcissus poeticus (L.) species stands out from other narcissus due to its unique ornamental and sensory values. In our experiment, the flowers of N. poeticus were harvested at five different ...stages. After cutting, the plants were placed directly at room temperature or at 4 °C for 1 week. The opening status of the flowers was recorded every 12 h, and based on this, the ornamental value of the flowers was calculated. Additionally, the flower diameter, the scape and flower weight, the dry matter content, the water soluble K, Ca, and Mg content of the plant parts, and the chlorophyll content of the scapes were measured. The complete senescence of the stored flowers was noted to be earlier (after 6.6–7.5 days) than that of the fresh flowers (7.5–8.5 days) and depended on the bud stage at harvest. Flowers opened from all the buds, but the flower size was smaller in the early developmental harvest stage (a 10 mm reduction in diameter and a 0.38 g reduction in fresh weight between the green bud and the large white bud harvest stages). Cold storage delayed further bud development and reduced the flower size in several cases, but it increased the maximum decorative value of the flowers for those flowers that had been harvested as big white buds.
The work under the conditions of the micropark experience analyzes the impact of the method of application of mineral wool and various reclamation methods for the physicochemical changes in the ...soilless track devastated by mining sulfur. Our results showed that the tested waste, as well as its manner of application to the soil, had a positive influence on the physical and chemical properties of degraded soils. The most beneficial impact was recorded in the conditions of association of the mineral wool in the top layer of the soil (0–25 cm) of the sewage sludge.
This study focused on the identification and elimination of endophytic bacterial contaminations during
propagation of European bladdernut (
). Axillary shoots were propagated on Murashige and Skoog ...medium with 20 mg ∙ dm
FeEDDHA, 5 μM BA and 0.5 μM NAA at 20/18°C (day/night) and a 16-h photoperiod. Clouding by endophytic bacterial colonies was observed where shoots contacted the media. Bacteria were isolated and separated by repeated streaking as two strains. Gram staining revealed that both strains were Gram-negative. The colonies were very precisely identified as
, strain ATCC 17909, and
, strain DSM 5687, using VITEK
2—a rapid bacterial identification system—and the 16S rRNA gene sequencing method. The agar disc-diffusion test proved that both bacterial strains were susceptible to 13 antibiotics (out of 25 tested), derived from the groups of fluoroquinolones, aminoglycosides and tetracyclines. Doxycycline or gentamicin (100–300 mg ∙ dm
) was added to the
shoot propagation medium to eliminate bacteria. Gentamicin 100 mg ∙ dm
showed the best effect, inhibiting the growth of endogenous bacteria (63%) when applied in the medium for 4 weeks. After the following transfer to media without antibiotics, shoots developed axillary buds and bacterial colonies were not observed.
PDF
