Today the use of Construction and Demolition Materials (CDM) can be considered as a suitable solution for the construction or the rehabilitation of road pavements. In this context, it is central to ...minimizing waste production, favoring the reuse through new production cycles to replace virgin natural raw materials. As illustrated in this study, steel slag has mechanical properties that justify its use as aggregate in the manufacture of bituminous mixes. In road construction, their use is focused on the substitution of fine aggregate and filler in bituminous mixtures. Mechanical characterizations, Marshall stability and indirect tensile resilient modulus (ITSM) tests were used to evaluate the laboratory performance of the mixtures. The research aims are to provide the use of these materials for the construction of the entire road pavement structure; in this study authors used these materials both in the characterization of cementitious layers and in those with bituminous conglomerate. In both cases, the use of steel slag has favored an increase of stiffness in the mixtures.
Aim of this study is to evaluate any differences in VWF antigen, VWF activity and ADAMTS-13 activity before and after successful and non-successful Percutaneous Transluminal Angioplasty (PTA) in ...subjects with type 2 diabetes (T2DM) complicated by Chronic limb-threatening ischemia (CLTI) in diabetic foot vasculopathy.
In this prospective observational pilot study, we enrolled 35 T2DM subjects who underwent lower limb PTA. Transcutaneous oximetry was performed in all patients before and 6 weeks after PTA. The change in oxygen partial pressure (TcpO2) before and after PTA was expressed as TcpO2-delta (ΔTcpO2). VWF antigen, VWF activity and ADAMTS-13 activity were measured before and 6 weeks after PTA; changes were expressed as delta and ratio from baseline.
Subjects with ∆TcpO2 < 15 mmHg presented higher ΔVWF activity (p = 0.050) and lower ADAMTS-13 activity ratio (p = 0.080). Subjects with ∆TcpO2 < 30 mmHg showed lower ADAMTS-13 activity Δ and ratio (p = 0.028).
VWF antigen levels and VWF activity may potentially affect PTA outcome. Higher levels of VWF could derive from VWF release as consequence of PTA-induced mechanical endothelial damage and/or oxidative stress-induced modifications of VWF structure with impairment of VWF-ADAMTS13 interactions.
We evaluate and compare the sensitivity of gold nanodisks on silica substrates and nanoholes made in silica-supported gold films, two of the most common sensor structures used in plasmonic ...biosensing. An alumina overcoat was applied by atomic layer deposition (ALD) to precisely control the interfacial refractive index and determine the evanescent plasmonic field decay length. The results are in good agreement with analytical models and biomolecular binding experiments for the two substrates. We found that nanodisks outperform nanoholes for thin dielectric coatings (<∼20 nm), while the opposite holds true for thicker coatings (>∼20 nm). The optimum nanoplasmonic transducer element for a given biorecognition reaction can be chosen based on experimentally determined bulk sensitivities/noise levels and theoretically estimated evanescent field decay lengths.
In recent years, the use of crumb rubber as modifier or additive within asphalt concretes has allowed obtaining mixtures able to bind high performances to recovery and reuse of discarded tires. To ...date, the common technologies that permit the reuse of rubber powder are the wet and dry ones. In this paper, a dry-hybrid technology for the production of Stone Mastic Asphalt mixtures is proposed. It allows the use of the rubber powder as filler, replacing part of the limestone one. Fillers are added and mixed with a high workability bitumen, modified with SBS (styrene-butadiene-styrene) polymer and paraffinic wax. The role of rubber powder and limestone filler within the bituminous mastic has been investigated through two different approaches. The first one is a rheological approach, which comprises a macro-scale laboratory analysis and a micro-scale DEM simulation. The second, instead, is a performance approach at high temperatures, which includes Multiple Stress Creep Recovery tests. The obtained results show that the rubber works as filler and it improves rheological characteristics of the polymer modified bitumen. In particular, it increases stiffness and elasticity at high temperatures and it reduces complex modulus at low temperatures.
Diabetes technology has proliferated extensively over the past few decades with vast ameliorations in glucose monitoring and in insulin delivery systems. From a treatment based on daily insulin ...injections, we have moved to increasingly advanced technologies. Despite such advancements which have allowed better glycemic control, decreased diabetes-related complications, and improved the quality of life among diabetic patients, it has left many individuals unsatisfied with the current rate of commercial artificial pancreas development, stemming the need for further research into novel technologies. Accordingly, the Juvenile Diabetes Research Foundation has marked three generations for the development of an artificial pancreas comprising historical landmarks and future prospects which aim to produce an advanced technological system that attempts to mimic the endogenous pancreas, eliminating the need for user input. This review presents a synopsis of the development and evolution of insulin pumps, starting with the earliest technologies available such as continuous subcutaneous insulin infusion and continuous glucose monitoring as separate components, to currently available integrated advanced closed-loop hybrid systems and possible future technologies. The aim of the review is to provide insight of the advantages and limitations of past and currently available insulin pumps with the hope of driving research into novel technologies that attempt to mimic endogenous pancreatic function as closely as possible.
Asphalt concrete mixtures are composed of two main components: aggregates and binder. The fraction of aggregates passing through the 63μm sieve is traditionally considered as filler. During years, ...several researches have shown the importance and the influence of filler in controlling the physical and mechanical properties of Hot Mix Asphalts (HMAs). The main objective of this research is to investigate and to assess the effects given by the use of Mining Waste Powder (MWP) within HMAs in total substitution of traditional limestone filler. The MWP used in this study is a residual of the tungsten extraction process in Panasqueira (Portugal) mine. The evaluation of properties conferred by the presence of the MWP filler within asphalt mixtures is based on a physical and mechanical laboratory characterization. For this purpose, tests have been performed both on bituminous mastics and on HMAs. Results indicate that the use of MWP in total substitution of limestone filler does not negatively affect the performances of HMAs and their bituminous mastics.
Aiming to study the rheo-mechanical effects of fine crumb rubber into gap graded stone mastic asphalt (SMA) mixtures, a multi-scale experimental approach was adopted. Therefore, in the perspective of ...the reuse of end of life tires’ in asphalt layers, the adopted new dry-hybrid technology effects have been investigated from the mastic, mortar and mixture points of view. The new rubberized asphalt production technology allows the use of rubber powder as filler, the rubber amount optimization being validated through multi-scale performance tests. Mastics and mortars’ complex modulus measured with dynamic shear and torsional tests were related to the mixture stiffness modulus recorded in direct tension-compression mode. The rheological properties of mastic are strictly influenced by the rubber presence, and consequently the asphalt mixtures stiffness and thermo-sensitivity are connected to the mastic and mortar rheo-mechanical behavior. Results are consistent through the adopted approach and reveal that with the new dry-hybrid technology, overcoming the wet and dry limits, it seems to be possible obtaining more durable and eco-friendly bituminous pavement layers.
Spitzoid melanoma is very rare tumour in the pediatric population, with clinical and non-uniform behaviour, different from adult melanoma 1. It can be difficult to differentiate an atypical Spitz ...nevus from a Spitzoid melanoma, resulting in diagnostic problems. In addition, in our clinical case, the COVID-19pandemiccaused significant delays both in the diagnosis and in the surgical treatment of our patient.
We present the clinical case of a 4-year-old child suffering from a localized polypoid cutaneous neoformation on the dorsum of the left hand, which started immediately before the lockdown and steadily increased during the COVID-19 pandemic. After a general clinical framing, the child underwent an excisional biopsy at our Department of Plastic and Reconstructive Surgery, at the Policlinico of Foggia. Subsequently, two independent anatomic pathology groups examined the specimen. Definitive diagnosis was made only after careful genetic analysis in combination with supporting histological and immunohistochemical examinations.
This clinical case shows how during the pandemic we have been facing advanced forms of tumours, compared to the previous period and highlight show an interdisciplinary and multicenter collaboration allowed a quick diagnosis of certainty, demonstrating the utility of molecular pathology as a fundamental aid in clinical/surgical practice.
In recent years, an increasing enthusiasm has been observed towards artificial intelligence and machine learning, involving different areas of medicine. Among these, although still in the embryonic ...stage, the dermatopathological field has also been partially involved, with the attempt to develop and train algorithms that could assist the pathologist in the differential diagnosis of complex melanocytic lesions. In this article, we face this new challenge of the modern era, carry out a review of the literature regarding the state of the art and try to determine promising future perspectives.
The antigenic repertoire presented by MHC molecules is generated by the antigen processing and presentation (APP) pathway. We analyzed the evolutionary history of 45 genes involved in APP at the ...inter- and intra-species level. Results showed that 11 genes evolved adaptively in mammals. Several positively selected sites involve positions of fundamental importance to the protein function (e.g. the TAP1 peptide-binding domains, the sugar binding interface of langerin, and the CD1D trafficking signal region). In CYBB, all selected sites cluster in two loops protruding into the endosomal lumen; analysis of missense mutations responsible for chronic granulomatous disease (CGD) showed the action of different selective forces on the very same gene region, as most CGD substitutions involve aminoacid positions that are conserved in all mammals. As for ERAP2, different computational methods indicated that positive selection has driven the recurrent appearance of protein-destabilizing variants during mammalian evolution. Application of a population-genetics phylogenetics approach showed that purifying selection represented a major force acting on some APP components (e.g. immunoproteasome subunits and chaperones) and allowed identification of positive selection events in the human lineage. We also investigated the evolutionary history of APP genes in human populations by developing a new approach that uses several different tests to identify the selection target, and that integrates low-coverage whole-genome sequencing data with Sanger sequencing. This analysis revealed that 9 APP genes underwent local adaptation in human populations. Most positive selection targets are located within noncoding regions with regulatory function in myeloid cells or act as expression quantitative trait loci. Conversely, balancing selection targeted nonsynonymous variants in TAP1 and CD207 (langerin). Finally, we suggest that selected variants in PSMB10 and CD207 contribute to human phenotypes. Thus, we used evolutionary information to generate experimentally-testable hypotheses and to provide a list of sites to prioritize in follow-up analyses.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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