Abstract
High-resolution Doppler spectroscopy provides an avenue to study the atmosphere of both transiting and non-transiting planets. This powerful method has also yielded some of the most robust ...atmospheric detections to date. Currently, high-resolution Doppler spectroscopy detects atmospheric signals by cross-correlating observed data with a model atmospheric spectrum. This technique has been successful in detecting various molecules such as H2O, CO, HCN and TiO, as well as several atomic species. Here we present an alternative method of performing high-resolution Doppler spectroscopy, using a technique known as Doppler tomography. We present an analysis of HD 179949 b using Doppler tomography and provide Doppler tomograms confirming previous detections of CO at 2.3 μm and H2O at both 2.3 μm and 3.5 μmwithin the atmosphere of HD 179949 b, showing significantly lower background noise levels when compared to cross-correlation methods applied to the same data. We also present a novel detection of H2O at 2.1 μm as well as a tentative detection of CO on the night side of the planet at 2.3 μm. This represents the first observational evidence for molecular absorption in the night-side emission spectrum of an exoplanet using Doppler spectroscopy.
Here we report that a synthesised form of a naturally occurring chemical (a butenolide, 3-methyl-2H-furo2,3-cpyran-2-one) found in smoke can stimulate seedling emergence of the economically important ...weed species Avena fatua L. (Poaceae), Arctotheca calendula (L.) Levyns (Asteraceae), Brassica tournefortii Gouan (Brassicaceae), and Raphanus raphanistrum L. (Brassicaceae) under field conditions at rates equivalent to 2-20 g/ha a.i. The butenolide also stimulates germination of freshly collected seeds from wild populations of these species, as well as those of Sisymbrium orientale L. (Brassicaceae), Hordeum leporinum Link (Poaceae) and Echium plantagineum L. (Boraginaceae) under laboratory conditions, consistently greater than that of smoke water. Experiments using B. tournefortii seeds collected from several locations across Western Australia and in different growing seasons found that these factors significantly influence the butenolide response, implying a role of the maternal environment in seed germination/dormancy characteristics. This research highlights the potential of butenolide as an agent for broad acre weed control and land restoration.
In degraded dryland systems, native plant community re-establishment following disturbance is almost exclusively carried out using seeds, but these efforts commonly fail. Much of this failure can be ...attributed to the limited understanding of seed dormancy and germination traits. We undertook a systematic classification of seed dormancy of 26 species of annual and perennial forbs and shrubs that represent key, dominant genera used in restoration of the Great Basin ecosystem in the western United States. We examined germination across a wide thermal profile to depict species-specific characteristics and assessed the potential of gibberellic acid (GA
) and karrikinolide (KAR
) to expand the thermal germination envelope of fresh seeds. Of the tested species, 81% produce seeds that are dormant at maturity. The largest proportion (62%) exhibited physiological (PD), followed by physical (PY, 8%), combinational (PY + PD, 8%) and morphophysiological (MPD, 4%) dormancy classes. The effects of chemical stimulants were temperature- and species-mediated. In general, mean germination across the thermal profile was improved by GA
and KAR
for 11 and five species, respectively. We detected a strong germination response to temperature in freshly collected seeds of 20 species. Temperatures below 10 °C limited the germination of all except Agoseris heterophylla, suggesting that in their dormant state, the majority of these species are thermally restricted. Our findings demonstrate the utility of dormancy classification as a foundation for understanding the critical regenerative traits in these ecologically important species and highlight its importance in restoration planning.
Paranthropus boisei was first described in 1959 based on fossils from the Olduvai Gorge and now includes many fossils from Ethiopia to Malawi. Knowledge about its postcranial anatomy has remained ...elusive because, until recently, no postcranial remains could be reliably attributed to this taxon. Here, we report the first associated hand and upper limb skeleton (KNM-ER 47000) of P. boisei from 1.51 to 1.53 Ma sediments at Ileret, Kenya. While the fossils show a combination of primitive and derived traits, the overall anatomy is characterized by primitive traits that resemble those found in Australopithecus, including an oblique scapular spine, relatively long and curved ulna, lack of third metacarpal styloid process, gracile thumb metacarpal, and curved manual phalanges. Very thick cortical bone throughout the upper limb shows that P. boisei had great upper limb strength, supporting hypotheses that this species spent time climbing trees, although probably to a lesser extent than earlier australopiths. Hand anatomy shows that P. boisei, like earlier australopiths, was capable of the manual dexterity needed to create and use stone tools, but lacked the robust thumb of Homo erectus, which arguably reflects adaptations to the intensification of precision grips and tool use. KNM-ER 47000 provides conclusive evidence that early Pleistocene hominins diverged in postcranial and craniodental anatomy, supporting hypotheses of competitive displacement among these contemporaneous hominins.
We present DES13S2cmm, the first spectroscopically-confirmed superluminous supernova (SLSN) from the Dark Energy Survey (DES). We briefly discuss the data and search algorithm used to find this event ...in the first year of DES operations, and outline the spectroscopic data obtained from the European Southern Observatory (ESO) Very Large Telescope to confirm its redshift (z = 0.663 ± 0.001 based on the host-galaxy emission lines) and likely spectral type (Type I). Using this redshift, we find
$M^{\rm peak}_U=-21.05^{+0.10}_{-0.09}$
for the peak, rest-frame U-band absolute magnitude, and find DES13S2cmm to be located in a faint, low-metallicity (sub-solar), low stellar-mass host galaxy (log (M/M⊙) = 9.3 ± 0.3), consistent with what is seen for other SLSNe-I. We compare the bolometric light curve of DES13S2cmm to 14 similarly well-observed SLSNe-I in the literature and find that it possesses one of the slowest declining tails (beyond +30 d rest-frame past peak), and is the faintest at peak. Moreover, we find the bolometric light curves of all SLSNe-I studied herein possess a dispersion of only 0.2–0.3 mag between +25 and +30 d after peak (rest frame) depending on redshift range studied; this could be important for ‘standardizing’ such supernovae, as is done with the more common Type Ia. We fit the bolometric light curve of DES13S2cmm with two competing models for SLSNe-I – the radioactive decay of 56Ni, and a magnetar – and find that while the magnetar is formally a better fit, neither model provides a compelling match to the data. Although we are unable to conclusively differentiate between these two physical models for this particular SLSN-I, further DES observations of more SLSNe-I should break this degeneracy, especially if the light curves of SLSNe-I can be observed beyond 100 d in the rest frame of the supernova.
Vegetation within some parts of Western Australia has been degraded by resource extraction, and ecological restoration is necessary to prevent erosion and reinstate plant biodiversity. Two ...restoration approaches, seed broadcasting and planting of seedlings, were tested with plant species (Acacia tetragonophylla F. Muell., Atriplex bunburyana F. Muell. and Solanum orbiculatum Poir.) known to have been dominant prior to mining activities in the World Heritage Area at Shark Bay. For broadcast seeding, soil raking and/or ripping increased seedling emergence, but only after sufficient rainfall. Survival of A. bunburyana seedlings (≤92%) was higher than A. tetragonophylla (≤13%) almost two years after planting and soil ripping partly alleviated soil impedance and resulted in increased seedling survival. Shoot pruning, fertiliser and moisture retaining gel had a reduced or detrimental effect on survival. Seedling survival differed between the three experimental sites, with electrical conductivity being the most noted soil difference between the sites. Restoration in the arid environs of the World Heritage Area at Shark Bay in Western Australia is challenging, but this study shows that seedling establishment is technically feasible and provides methodology useful to other arid restoration projects.
► Soil preparation increases seedling emergence from seed broadcasting. ► Soil ripping prior to planting may alleviate compaction and increase seedling survival. ► Seedling emergence and survival in the arid zone should be assessed over several years due to rainfall variation.
Revegetation of disturbed land, particularly in arid environments, is often hindered by low seedling establishment. Information on seed biology and germination cues of keystone species is lacking, ...particularly in arid Australia; a major zone for mining developments. This study investigated seed characteristics and germination of 18 common species required for rehabilitation of disturbed areas at Shark Bay Salt in the Shark Bay World Heritage Area, Western Australia. Untreated seeds of seven species (
Aphanopetalum clematideum,
Atriplex bunburyana,
Austrostipa elegantissima,
Melaleuca cardiophylla,
Pembertonia latisquamea,
Rhagodia baccata,
Salsola tragus) exhibited high germination percentages. Seeds of two species (
Acacia tetragonophylla,
Stylobasium spathulatum) had low imbibition, which increased with hot-water treatment, hence require scarification for germination. Gibberellic acid, smoke water and karrikinolide (a butenolide isolated from smoke) substantially increased germination percentages of three species (
Anthocercis littorea,
Diplolaena grandiflora,
Solanum orbiculatum). Seeds of the remaining six species (
Dioscorea hastifolia,
Eremophila oldfieldii,
Nitraria billardierei,
Ptilotus exaltatus,
Thryptomene baeckeacea,
Zygophyllum fruticulosum) had low germination percentages regardless of treatment. Most species germinated equally well at 26/13
°C and 33/18
°C, however seven species had improved performance at 26/13
°C. This study is significant to land managers and conservation agencies with an interest in optimising germination of arid zone seeds for restoration.
BACKGROUND: Preclinical studies in animal models have demonstrated tumor regression following intratumoral administration of an adenovirus vector containing wild-type p53 complementary DNA (Ad-p53). ...Therefore, in a phase I clinical trial, we administered Ad-p53 to 28 patients with non-small-cell lung cancer (NSCLC) whose cancers had progressed on conventional treatments. METHODS: Patients received up to six, monthly intratumoral injections of Ad-p53 by use of computed tomography-guided percutaneous fine-needle injection (23 patients) or bronchoscopy (five patients). The doses ranged from 106 plaque-forming units (PFU) to 1011 PFU. RESULTS: Polymerase chain reaction (PCR) analysis showed the presence of adenovirus vector DNA in 18 (86%) of 21 patients with evaluable posttreatment biopsy specimens; vector-specific p53 messenger RNA was detected by means of reverse transcription-PCR analysis in 12 (46%) of 26 patients. Apoptosis (programmed cell death) was demonstrated by increased terminal deoxynucleotide transferase-mediated biotin uridine triphosphate nick-end labeling (TUNEL) staining in posttreatment biopsy specimens from 11 patients. Vector-related toxicity was minimal (National Cancer Institute's Common Toxicity Criteria: grade 3 = one patient; grade 4 = no patients) in 84 courses of treatment, despite repeated injections (up to six) in 23 patients. Therapeutic activity in 25 evaluable patients included partial responses in two patients (8%) and disease stabilization (range, 2-14 months) in 16 patients (64%); the remaining seven patients (28%) exhibited disease progression. CONCLUSIONS: Repeated intratumoral injections of Ad-p53 appear to be well tolerated, result in transgene expression of wild-type p53, and seem to mediate antitumor activity in a subset of patients with advanced NSCLC.
BACKGROUND AND AIMS: Following a period of burial, more Actinotus leucocephalus (Apiaceae) and Tersonia cyathiflora (Gyrostemonaceae) seeds germinate in smoke water. The main aim of this study was to ...determine whether these fire-ephemeral seeds exhibit annual dormancy cycling during burial. This study also aimed to determine the effect of dormancy alleviation on the range of light and temperature conditions at which seeds germinate, and the possible factors driving changes in seed dormancy during burial. METHODS: Seeds were collected in summer, buried in soil in mesh bags in autumn and exhumed every 6 months for 24 months. Germination of exhumed and laboratory-stored (15 °C) seeds was assessed at 20 °C in water or smoke water. Germination response to light or dark conditions, incubation temperature (10, 15, 20, 25 and 30 °C), nitrate and gibberellic acid were also examined following burial or laboratory storage for 24 months. In the laboratory seeds were also stored at various temperatures (5, 15, 37 and 20/50 °C) for 1, 2 and 3 months followed by germination testing in water or smoke water. KEY RESULTS: The two species exhibited dormancy cycling during soil burial, producing low levels of germination in response to smoke water when exhumed in spring and high levels of germination in autumn. In autumn, seeds germinated in both light and dark and at a broader range of temperatures than did laboratory-stored seeds, and some Actinotus leucocephalus seeds also germinated in water alone. Dormancy release of Actinotus leucocephalus was slow during dry storage at 15 °C and more rapid at higher temperatures (37 and 20/50 °C); weekly wet/dry cycles further accelerated the rate of dormancy release. Cold stratification (5 °C) induced secondary dormancy. By contrast, no Tersonia cyathiflora seeds germinated following any of the laboratory storage treatments. CONCLUSIONS: Temperature and moisture influence dormancy cycling in Actinotus leucocephalus seeds. These factors alone did not simulate dormancy cycling of Tersonia cyathiflora seeds under the conditions tested.
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