Three‐dimensional (3D) printing is an emerging technique that has shown promising success in engineering human tissues in recent years. Further development of vat‐photopolymerization printing ...modalities has significantly enhanced the complexity level for 3D printing of various functional structures and components. Similarly, the development of microfluidic chip systems is an emerging research sector with promising medical applications. This work demonstrates the coupling of a digital light processing (DLP) printing procedure with a microfluidic chip system to produce size‐tunable, 3D‐printable porosities with narrow pore size distributions within a gelatin methacryloyl (GelMA) hydrogel matrix. It is found that the generation of size‐tunable gas bubbles trapped within an aqueous GelMA hydrogel‐precursor can be controlled with high precision. Furthermore, the porosities are printed in two‐dimensional (2D) as well as in 3D using the DLP printer. In addition, the cytocompatibility of the printed porous scaffolds is investigated using fibroblasts, where high cell viabilities as well as cell proliferation, spreading, and migration are confirmed. It is anticipated that the strategy is widely applicable in a range of application areas such as tissue engineering and regenerative medicine, among others.
A digital light processing printing procedure is coupled with a microfluidic chip system to produce size‐tunable, 3D‐printable porosities with narrow pore size distributions within a gelatin methacryloyl hydrogel matrix, showing favorable cytocompatibility of the printed porous scaffolds. The strategy is anticipated to be broadly usable in a range of biomedical applications.
The identification of pollen in an automated way will accelerate different tasks and applications of palynology to aid in, among others, climate change studies, medical allergies calendar, and ...forensic science. The aim of this paper is to develop a system that automatically captures a hundred microscopic images of pollen and classifies them into the 12 different species from Lagunera Region, Mexico. Many times, the pollen is overlapping on the microscopic images, which increases the difficulty for its automated identification and classification. This paper focuses on a method to segment the overlapping pollen. First, the proposed method segments the overlapping pollen. Second, the method separates the pollen based on the mean shift process (100% segmentation) and erosion by H-minima based on the Fibonacci series. Thus, pollen is characterized by its shape, color, and texture for training and evaluating the performance of three classification techniques: random tree forest, multilayer perceptron, and Bayes net. Using the newly developed system, we obtained segmentation results of 100% and classification on top of 96.2% and 96.1% in recall and precision using multilayer perceptron in twofold cross validation.
Colon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, ...specifically bacterial cyclomodulin positivity (CM
). Unfortunately, there are no studies from Mexico that detail the presence of bacterial CM
in patients with colon diseases. We therefore performed a comprehensive study to investigate the associations and prevalence of cyclomodulin-positive Diarrheagenic
(DEC)
non-DEC, and
spp. strains isolated from Mexican subjects with colon diseases. In this work, we analyzed 43 biopsies, 87 different bacteria were isolated, and
was the most frequently noted, followed by
spp., and
spp.
, non-DEC, and EPEC belonging to phylogroup B2 were the most prevalent. More than 80% of
and
were CM
.
,
,
, and
were identified.
was associated with non-DEC,
and its combinations with EPEC, as well as
and
with
. Lastly, all the CM
bacteria were resistant to at least one antibiotic (34% were MDR, and 48% XDR). In conclusion, the high prevalence of bacterial CM
in colon disease patients suggests that these bacteria play an important role in the genesis of these diseases.
Abstract This study aimed to assess the psychometric properties of the Spanish version of the Problem Areas in Diabetes (PAID-SP) Scale. Data were collected from patients with type 1 and type 2 ...diabetes. The findings suggested that the PAID-SP is a reliable and valid measure of diabetes-specific emotional problems.
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Paraffin-embedded tissues have been used for research and therapeutic applications for decades, as they represent a valuable tool in histology and for molecular analysis, as well as ...being a way to preserve tissue samples for long periods at a low cost. For tissues such as the liver, lungs, kidney, heart or brain, there are many protocols available, already optimized. The purpose of this work is to optimize and simplify the protocols already available to take a single blastocyst from a mouse, fix it and embed it into a paraffin block without using gelatin, to then perform histological cuts using a microtome, with no need of sophisticated equipment or trained personnel.
•The protocol presented here preserves well the morphology of the blastocyst.•Paraffin-embedded sections of the sample can be used for studies such as in situ hybridization, immunohistochemistry, enzyme histochemistry, DNA, RNA or protein extractions, analysis of biomarkers, characterization of surface markers of stem cells integrated into the embryo, to prepare histological material for educational purposes, etc.•Some of these studies could represent a valuable source of new information for the field of reproductive biology.
•Age-dependent increase in Ca2+d and Na+d in mdx cardiomyocytes.•Gadolinium significantly reduced both Ca2+d and Na+d at all ages.•IP3-pathway inhibition reduced cations concentrations in dystrophic ...cardiomyocytes.
Duchenne muscular dystrophy (DMD) is a lethal X-inherited disease caused by dystrophin deficiency. Besides the relatively well characterized skeletal muscle degenerative processes, DMD is also associated with a dilated cardiomyopathy that leads to progressive heart failure at the end of the second decade. The aim of the present study was to characterize the diastolic Ca2+ concentration (Ca2+d) and diastolic Na+ concentration (Na+d) abnormalities in cardiomyocytes isolated from 3-, 6-, 9-, and 12-month old mdx mice using ion-selective microelectrodes. In addition, the contributions of gadolinium (Gd3+)-sensitive Ca2+ entry and inositol triphosphate (IP3) signaling pathways in abnormal Ca2+d and Na+d were investigated. Our results showed an age-dependent increase in both Ca2+d and Na+d in dystrophic cardiomyocytes compared to those isolated from age-matched wt mice. Gd3+ treatment significantly reduced both Ca2+d and Na+d at all ages. In addition, blockade of the IP3-pathway with either U-73122 or xestospongin C significantly reduced ion concentrations in dystrophic cardiomyocytes. Co-treatment with U-73122 and Gd3+ normalized both Ca2+d and Na+d at all ages in dystrophic cardiomyocytes. These data showed that loss of dystrophin in mdx cardiomyocytes produced an age-dependent intracellular Ca2+ and Na+ overload mediated at least in part by enhanced Ca2+ entry through Gd3+ sensitive transient receptor potential channels (TRPC), and by IP3 receptors.
Duchenne muscular dystrophy (DMD) is a lethal X-inherited disease caused by dystrophin deficiency. Besides the relatively well characterized skeletal muscle degenerative processes, DMD is also ...associated with a dilated cardiomyopathy that leads to progressive heart failure at the end of the second decade. The aim of the present study was to characterize the diastolic Ca(2+) concentration (Ca(2+)d) and diastolic Na(+) concentration (Na(+)d) abnormalities in cardiomyocytes isolated from 3-, 6-, 9-, and 12-month old mdx mice using ion-selective microelectrodes. In addition, the contributions of gadolinium (Gd(3+))-sensitive Ca(2+) entry and inositol triphosphate (IP3) signaling pathways in abnormal Ca(2+)d and Na(+)d were investigated. Our results showed an age-dependent increase in both Ca(2+)d and Na(+)d in dystrophic cardiomyocytes compared to those isolated from age-matched wt mice. Gd(3+) treatment significantly reduced both Ca(2+)d and Na(+)d at all ages. In addition, blockade of the IP3-pathway with either U-73122 or xestospongin C significantly reduced ion concentrations in dystrophic cardiomyocytes. Co-treatment with U-73122 and Gd(3+) normalized both Ca(2+)d and Na(+)d at all ages in dystrophic cardiomyocytes. These data showed that loss of dystrophin in mdx cardiomyocytes produced an age-dependent intracellular Ca(2+) and Na(+) overload mediated at least in part by enhanced Ca(2+) entry through Gd(3+) sensitive transient receptor potential channels (TRPC), and by IP3 receptors.
Research update for articles published in EJCI in 2015 Al‐Daghri, Nasser M.; Batzel, Jerry J.; Burgmann, Heinz ...
European journal of clinical investigation,
October 2017, 2017-10-00, 20171001, Letnik:
47, Številka:
10
Journal Article
► Runway Overrun-Undershoot risk depends on multiple factors and operating conditions. ► Risk Models allows assessing risk level when safety areas standards are not meet. ► Runway Overrun-Undershoot ...risk Models are supported by historical data on accidents. ► This methods offer high quality results from limited time, computing power and data.
The risks of landing overrun (LDOR – LanDing OverRun), Take-off Overrun (TOOR – Take-Off OverRun) and landing undershoot (LDUS – LanDing UnderShoot) are dependent on multiple factors related to operating conditions. These include wind, runway surface conditions, landing or take-off distances required, the presence of obstacles, runway distance available, the existence and dimensions of runway safety areas.
In this paper we propose risk models for runway overrun and landing undershoot, using a probabilistic approach. These models are supported by historical data on accidents in the area around the runway and will enable us to determine if the risk level is acceptable or whether action must be taken to mitigate such risks at a given airport. Furthermore, these models permit comparison of the results of different risk mitigation actions in terms of operational risk and safety.
The principal advantage of this method is the high quality results obtained for a limited investment in terms of time, computing power and data. As such the method is extremely practical and easy to apply in aerodrome planning, development and operation.
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