In Table 3, the letter R was mistakenly removed from the beginning of each SNP during the production process. Download corrected item. ...https://doi.org/10.1371/annotation/7b3edc45-39c7-416b-a7dc-124f4846303d.t003.cn Citation: Sharma NK, Langberg KA, Mondal AK, Das SK (2013) Correction: Phospholipid Biosynthesis Genes and Susceptibility to Obesity: Analysis of Expression and Polymorphisms.
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"In the present paper, we study three-dimensional quasi-Sasakian manifolds admitting the Schouten-van Kampen connection. We characterize quasi-Sasakian manifolds and nd certain curvature properties ...with respect to the Schouten-van Kampen connection. Finally, we construct an example of a three-dimensional quasi-Sasakian manifold admitting the Schouten-van Kampen connection which veri es the results discussed in the present paper."
Indoleamine 2,3 dioxygenase-1 (IDO-1) is an enzyme in the kynurenine pathway which augments tumor-induced immune tolerance. Previous studies in childhood acute myeloid leukemia (AML) have shown a ...negative correlation of IDO-1 mRNA expression with outcomes. The aim of our study was to develop a practical and objective immunohistochemical technique to quantify IDO-1 expression on diagnostic bone marrow biopsies of AML patients in order to facilitate its use in routine clinical practice. IDO-1 mRNA was extracted from diagnostic bone marrow specimens from 29 AML patients. IDO-1 protein expression was assessed in 40 cases via immunohistochemistry and quantified by a novel 'composite IDO-1 score'. In a univariate analysis, higher age (p = 0.0018), male gender (p = 0.019), high risk cytogenetics (p = 0.002), higher IDO-1 mRNA (p = 0.005), higher composite IDO-1 score (p < 0.0001) and not undergoing allogeneic stem cell transplant (SCT, p = 0.0005) predicted poor overall survival. In a multivariate model that included the aforementioned variables, higher composite IDO-1 score (p = 0.007) and not undergoing allogeneic SCT (p = 0.007) was found to significantly predict poor outcomes. Further, patients who failed induction had higher composite IDO-1 score (p = 0.01). In conclusion, 'composite IDO-1 score' is a prognostic tool that can help identify a certain subset of AML patients with 'early mortality'. This unique subset of patients can potentially benefit from specific IDO-1 inhibitor therapy, currently in clinical trials.
1 Endocrinology Section, Medicine and Research Services, Central Arkansas Veterans Healthcare System, Little Rock; and 2 Division of Endocrinology and Metabolism, Department of Medicine, College of ...Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas
Submitted 10 April 2008
; accepted in final form 6 June 2008
Obesity and elevated cytokine secretion result in a chronic inflammatory state and may cause the insulin resistance observed in type 2 diabetes. Recent studies suggest a key role for endoplasmic reticulum stress in hepatocytes and adipocytes from obese mice, resulting in reduced insulin sensitivity. To address the hypothesis that thiazolidinediones, which improve peripheral insulin sensitivity, act in part by reducing the endoplasmic reticulum stress response, we tested subcutaneous adipose tissue from 20 obese volunteers treated with pioglitazone for 10 wk. We also experimentally induced endoplasmic reticulum stress using palmitate, tunicamycin, and thapsigargin in the human HepG2 liver cell line with or without pioglitazone pretreatment. We quantified endoplasmic reticulum stress response by measuring both gene expression and phosphorylation. Pioglitazone significantly improved insulin sensitivity in human volunteers ( P = 0.002) but did not alter markers of endoplasmic reticulum stress. Differences in pre- and posttreatment endoplasmic reticulum stress levels were not correlated with changes in insulin sensitivity or body mass index. In vitro, palmitate, thapsigargin, and tunicamycin but not oleate induced endoplasmic reticulum stress in HepG2 cells, including increased transcripts CHOP, ERN1, GADD34, and PERK, and increased XBP1 splicing along with phosphorylation of eukaryotic initiation factor eIF2 , JNK1, and c- jun . Although patterns of endoplasmic reticulum stress response differed among palmitate, tunicamycin, and thapsigargin, pioglitazone pretreatment had no significant effect on any measure of endoplasmic reticulum stress, regardless of the inducer. Together, our data suggest that improved insulin sensitivity with pioglitazone is not mediated by a reduction in endoplasmic reticulum stress.
insulin sensitivity; fatty acid; thiazolidinediones; type 2 diabetes; obesity
Address for reprint requests and other correspondence: S. C. Elbein or Swapan K. Das, Univ. of Arkansas for Medical Sciences, Endocrinology 111J-1/LR, John L. McClellan Memorial Veterans Hospital, 4300 W. 7th St., Little Rock, AR 72205 (e-mail: elbeinstevenc{at}uams.edu )
In the present paper, we study three-dimensional trans-Sasakian manifolds admitting the Schouten-van Kampen connection. Also, we have proved some results on $\phi$-projectively flat, ...$\xi-$projectively flat and $\xi-$concircularly flat three-dimensional trans-Sasakian manifold with respect to the Schouten-van Kampen connection. Locally $\phi-$symmetry trans-Sasakian manifolds of dimension three have been studied with respect to Schouten-van Kampen connection. Finally, we construct an example of a three-dimensional trans-Sasakian manifold admitting Schouten-van Kampen connection which verifies Theorem 4.1.
In the present paper, we study η-Ricci solitons on para-Kenmotsu manifolds with Codazzi type of the Ricci tensor. We study η-Ricci solitons on paraKenmotsu manifolds with cyclic parallel Ricci ...tensor. We also study η-Ricci solitons on ϕ-conformally semi-symmetric, ϕ-Ricci symmetric and conformally Ricci semi-symmetric para-Kenmotsu manifolds. Finally, we construct an example of a three-dimensional para-Kenmotsu manifold which admits η-Ricci solitons. KCI Citation Count: 0
In the present paper, we study three-dimensional f-Kenmotsu manifolds admitting the Schouten-Van Kampen connection. We study the concircular curvature tensor of a three-dimensional f-Kenmotsu ...manifold with respect to the Schouten-Van Kampen connection. Finally, we have cited an example of a three-dimensional f-Kenmotsu manifold admitting Schouten-Van Kampen connection which verify our results. KCI Citation Count: 0
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, led to unprecedented demands assigned to clinical diagnostic laboratories worldwide, forcing them to make significant ...changes to their regular workflow as they adapted to new diagnostic tests and sample volumes. Herein, we summarize the modifications/adaptation the laboratory had to exercise to cope with rapidly evolving situations in the current pandemic. In the first phase of the pandemic, the laboratory validated 2 reverse transcription polymerase chain reaction–based assays to test ∼1000 samples/day and rapidly modified procedures and validated various preanalytical and analytical steps to overcome the supply chain constraints that would have otherwise derailed testing efforts. Further, the pooling strategy was validated for wide-scale population screening using nasopharyngeal swab samples and saliva samples. The translational research arm of the laboratory pursued several initiatives to understand the variable clinical manifestations that this virus presented in the population. The phylogenetic evolution of the virus was investigated using next-generation sequencing technology. The laboratory has initiated the formation of a consortium that includes groups investigating genomes at the level of large structural variants, using genome optical mapping via this collaborative global effort. This article summarizes our journey as the laboratory has sought to adapt and continue to positively contribute to the unprecedented demands and challenges of this rapidly evolving pandemic.
In the present paper, biharmonic Legendre curves with respect to Schouten-Van Kampen connection have been studied on three-dimensional f-Kenmotsu manifolds. Locally $\phi $-symmetric Legendre curves ...on three-dimensional f-Kenmotsu manifolds with respect to Schouten-Van Kampen Connection have been introduced.Also slant curves have been studied on three-dimensional f-Kenmotsu manifolds with respect to Schouten-Van Kampen connection. Finally, we constract an example of a Legendre curve in a 3-dimensional f-Kenmotsu manifold.
Type 2 diabetes (T2D)-associated SNPs are more likely to be expression quantitative trait loci (eQTLs). The allelic expression imbalance (AEI) analysis is the measure of relative expression between ...two allelic transcripts and is the most sensitive measurement to detect cis-regulatory effects. We performed AEI screening to detect cis-regulators for genes expressed in transformed lymphocytes of 190 Caucasian (CA) and African American (AA) subjects to identify functional variants for T2D susceptibility in the chromosome 1q21-24 region of linkage. Among transcribed SNPs studied in 115 genes, significant AEI (P < 0.001) occurred in 28 and 30 genes in CA and AA subjects, respectively. Analysis of the effect of selected AEI-SNPs (≥10% mean AEI) on total gene expression further established the cis-eQTLs in thioesterase superfamily member-4 (THEM4) (rs13320, P = 0.027), and IGSF8 (rs1131891, P = 0.02). Examination of published genome-wide association data identified significant associations (P < 0.01) of three AEI-SNPs with T2D in the DIAGRAM-v3 dataset. Six AEI single nucleotide polymorphisms, including rs13320 (P = 1.35E-04) in THEM4, were associated with glucose homeostasis traits in the MAGIC dataset. Evaluation of AEI-SNPs for association with glucose homeostasis traits in 611 nondiabetic subjects showed lower AIRG (P = 0.005) in those with TT/TC genotype for rs13320. THEM4 expression in adipose was higher (P = 0.005) in subjects carrying the T allele; in vitro analysis with luciferase construct confirmed the higher expression of the T allele. Resequencing of THEM4 exons in 192 CA subjects revealed four coding nonsynonymous variants, but did not explain transmission of T2D in 718 subjects from 67 Caucasian pedigrees. Our study indicates the role of a cis-regulatory SNP in THEM4 that may influence T2D predisposition by modulating glucose homeostasis.
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