Acute kidney injury (AKI) diagnosis relies on plasma creatinine concentration (Crpl), a relatively insensitive, surrogate biomarker of glomerular filtration rate that increases only after significant ...damage befalls. However, damage in different renal structures may occur without increments in Crpl, a condition known as subclinical AKI. Thus, detection of alterations in other aspects of renal function different from glomerular filtration rate must be included in an integral diagnosis of AKI. With this aim, we adapted to and validated in rats (for preclinical research) the furosemide stress test (FST), a tubular function test hitherto performed only in humans. We also tested its sensitivity in detecting subclinical tubular alterations. In particular, we predisposed rats to AKI with 3 mg/kg cisplatin and subsequently subjected them to a triggering insult (ie, 50 mg/kg/d gentamicin for 6 days) that had no effect on nonpredisposed animals but caused an overt AKI in predisposed rats. The FST was performed immediately before adding the triggering insult. Predisposed animals showed a reduced response to the FST (namely, reduced furosemide-induced diuresis and K+ excretion), whereas nonpredisposed animals showed no alteration, compared to the controls. Computational modeling of epithelial transport of solutes and water along the nephrons applied to experimental data suggested that proximal tubule transport was only minimally reduced, the sodium-chloride symporter was upregulated by 50%, and the renal outer medullary potassium channel was downregulated by 85% in predisposed animals. In conclusion, serial coupling of the FST and computational modeling may be used to detect and localize subclinical tubular alterations.
Scope: trans‐Resveratrol (RES) and/(or) its metabolites exert many effects in vivo. Our aim was to study the metabolism and tissue distribution of RES using the pig, a mammal physiologically close to ...humans.
Methods and results: Forty‐seven tissues, organs and fluids were analyzed 6 h after intragastric RES administration (5.9 mg/kg body weight) using HPLC‐MS/MS. Twelve RES and seven dihydroresveratrol (DH‐RES) metabolites were detected. DH‐RES was the main metabolite in cecum, colon and rectum, whereas RES‐3‐O‐glucuronide was the most abundant one in fluids and organs. Approximately 74.5% of the total RES administered was recovered in the form of RES, DH‐RES and derived metabolites (65.1% along the gastrointestinal tract, 7.7% in urine, 1.2% in bile and 0.5% in organs). We report here, for the first time, the occurrence of RES ribosyl‐sulfate derivative, DH‐RES diglucuronide, DH‐RES sulfoglucuronide and DH‐RES disulfate as well as the metabolic profile of RES and DH‐RES in the aorta, lymph, lymph node, ovaries, uterus, cerebellum, pancreas, urinary bladder tissue, fat and muscle.
Conclusion: This study contributes to the clarification of the metabolism and tissue distribution of RES and could help to further understand the mechanisms underlying its effects.
•EVOOs were differentiated between green fruity and ripe fruity sensory classes.•Sensory and volatile analyses were developed and correlated.•Three analytical methods were assessed for volatile ...characterization of classes.•The advantages of PARADISe processing for volatile analyses were confirmed.•Some volatile compounds were identified as “green” or “ripe” fruity markers.
An objective sensory evaluation of extra virgin olive oil (EVOO), involving the chemical characterization of positive attributes, is of interest. These attributes are objectively divided, according to fruitiness, into “green” and “ripe” fruity. This work studied the differentiation in the volatile profile of EVOOs into these two classes, obtained by three analytical methods, including different extraction techniques and detectors and two data processing strategies, and their relation with sensory results. According to the results, each method allowed the characterization of the two classes, providing information on different volatile compounds, which increased in number through PARADISe software (14 more than the conventional processing). Moreover, some volatile compounds showed significant differences between the two classes, 16 highlighted by the variables with importance in projection (VIP) for green fruity (e.g. (Z)-3-hexen-1-ol, methyl ether) and 23 for ripe fruity EVOOs (e.g. (Z)-2-hexen-1-ol), which could be considered as useful markers to complement quality assessment.
According to the theory of practice architecture, every practice enacted in classrooms is a result of interaction between social, physical and spatial elements. In relation, from a ...practice-referenced perspective, it is necessary to know which teaching-learning implementation features could help teachers/coaches/researchers to assemble Teaching Games for Understanding (TGfU) interventions in relation to the institutional environment. Purpose: This review aimed to explore from a practice-referenced perspective how TGfU researchers reported their interventions based on the teaching-learning implementation features (intervention design as a function of the context, intervention length, lesson content, basic lesson elements, lesson alignment, teacher/coach experience with the approach, and lesson validation and treatment verification) and their association with learners' outcomes. Results: We found 20 studies that included some of the teaching-learning implementation features, but none of the studies included all of these features. We also found that studies of TGfU measured and reported learners' outcomes in a variety of ways. This creates difficulties for drawing conclusions about the relationships between the presence of teaching-learning implementation features and student learning outcomes. Conclusion: Further TGfU interventions should be planned to consider the following: (a) that lessons need to be designed as a function of the context; (b) the number of intervention lessons, their duration and the duration of each lesson task; (c) the concrete tactical and technique contents and goals per lesson; (d) the modified games, questions and achievable challenges as basic lesson elements; (e) the alignment between the basic lesson elements and the structure of lessons, based on the goals of each lesson; (f) that teachers/coaches need to have previous experience in TGfU and be trained on the specific study purpose; (g) that lessons should be validated before implementation and verified during intervention; (h) researchers should regulate the ways in which learners' outcomes are measured and reported within TGfU studies.
The methodology of sensory assessment (“panel test”) of virgin olive oil (VOO) is the sensory method included in international regulations and its application is compulsory in trading. Despite its ...application has been a success in authenticity of VOO quality, the application of panel test is not exempt of heated debates and controversies among olive oil actors that sometimes surpasses scientific arguments. Problems are consequence of some disagreements in the evaluation of the same VOOs from different testing laboratories, and today, there is a clamor for its enhancement from a considerable part of the industrial sector. The thin line that exists between the absence of sensory defects in extra‐VOOs and a very slight sensory defect that may be perceived in some VOOs by some tasters is in the origin of the controversy. Thus, this work is focuses on providing practical solutions to the current concerns of Panel Test after analyzing proposals evaluating aroma descriptors from chemistry, examining flaws of panel test and strengthening its weak points.
Practical Applications: The work describes a strategy that combines volatiles and sensory descriptors for avoiding disagreements with results provided by non‐recognized testing laboratories, and proposes a blueprint for improving the process of training tasters with reference materials.
Panel test of virgin olive oil (VOO) is the only sensory assessment included in international regulations. Although its application has been a success, some problems based on disagreements among sensory qualifications of VOOs by panel tests have been reported. The article proposes solutions and future challenges to enhance and strengthen panel test with the assistance of the volatile compounds.
Young players report that they dropout of organized football due to excessive emphasis on technical execution, low success, and the lack of autonomy and motivation experienced by players during ...training sessions. Purpose: To determine whether a TGfU intervention during a youth football program led players to improve in variables related to dropout. That means tactical-technical competence (decision-making, skill execution), success (successful game performance), autonomy (number of decisions made, player autonomy, number of game involvements, player participation), and motivation (enjoyment, intention to be physically active). Method: Twenty under-11-players and two coaches were recruited from 17 clubs. A pretest-posttest design with a multi-method approach was used. Coaches were trained and mentored in TGfU. Data were collected using Game Performance Assessment Instrument, enjoyment and intention to be physically active scales, and two focus groups with the players and the coaches. Results: Players improved in decision-making, skill execution, successful game performance, number of decisions made, number of game involvements, and intention to be physically active (p < .05). Participants attributed the results to the TGfU pedagogical features emphasized during the coaches' training and mentoring. Conclusion: Considering the reasons for dropout in football, in terms of excessive emphasis on technical execution, low success, and the lack of autonomy and motivation experienced by players, TGfU could be a useful pedagogical approach for teaching-learning organized youth football. The TGfU pedagogical features emphasized during coaches' training and mentoring could be crucial to obtain these results due to the fact that they were the sub-themes highlighted during the focus groups.
Freeze injuries in olives are responsible for the ‘frostbitten olives’ sensory defect that is sometimes detected in virgin olive oil. This defect is becoming one of the most common negative ...attributes today because climate change has modified weather patterns. The temperature changes can take place abruptly, with rapid freeze–thaw cycles, or gradually. These changes produce significant alterations in the quality of the oils. This study analyzed the volatile composition of virgin olive oils characterized with ‘frostbitten olives’ defect. The volatile information allowed grouping these oils into two types characterized with two different profiles. One of them is characterized by ‘soapy’ and ‘strawberry-like’ perceptions and the presence of two volatile compounds (ethyl 2-methyl butanoate and ethyl propanoate). The second profile is characterized by ‘wood’ and ‘humidity’ descriptors and a high concentration of two volatiles (pentanal and octanal). These results on volatiles explain the existence of two sensory profiles associated with the ‘frostbitten olives’ defect.
Background: Although a great deal of research has been undertaken from 1982 on TGfU, teachers consider its implementation to be complex and are reluctant to integrate it into their teaching practice. ...Furthermore, some TGfU literature does not suggest how it might be implemented and how problems might be overcome. On the other hand, a review of TGfU studies showed three shortcomings. First, most of them only compared TGfU with a technique-based approach in the school setting. Second, the studies did not follow the fidelity guidelines for models-based practice research and it is therefore difficult to contrast their results. Third, lesson design was not aligned on the principles of play. Thus, this study provide additional support for going forward from comparative studies in educational settings, and enacting an aligned TGfU unit of floorball contextualized in the principles of play.
Purpose: To verify whether pupils improved in variables related to performance and adherence after the enactment of an aligned TGfU unit.
Method: An eight lessons unit was designed, validated, and enacted using a mixed-methods quasi-experimental pre-test and post-test design. Participants were 41 pupils (23 boys and 18 girls; M = 11.73, SD = .66 years old) from two regular physical education classes with the same teacher. The teacher was trained in the TGfU approach. In pre-test and post-test assessments, data from decision-making, technical execution, cover, support, game performance, game involvement, enjoyment, perceived competence, and intention to be physically active were collected, using the Game Performance Assessment Instrument, two psychological scales, and two focus groups interviews with the pupils and the teacher. We analysed quantitative data using t-tests and qualitative data following an open and axial coding based on the pre-existing categories.
Results: Pupils improved in decision-making, technical execution, cover, support, game performance, game involvement, enjoyment, perceived competence, and intention to be physically active after implementation of the TGfU unit. The pupils and the teacher perceived improvement in all the variables analysed as a consequence of the intervention.
Conclusion: After the implementation of the aligned TGfU floorball unit, pupils improved in the variables related to performance and adherence. Based on participants' key comments, four aspects appeared to be essential for the success of the unit: (a) the lesson design; (b) collaboration from planning to teaching the unit between the researcher and the teacher; (c) the inclusion of learning tasks linked with cover and support; and (d) the use of two reflective periods that helped pupils to understand and apply new knowledge.
ABSTRACTBazuelo-Ruiz, B, Padial, P, García-Ramos, A, Morales-Artacho, AJ, Miranda, MT, and Feriche, B. Predicting maximal dynamic strength from the load-velocity relationship in squat exercise. J ...Strength Cond Res 29(7)1999–2005, 2015—The aim of this study was to develop a rapid indirect method to determine an individualʼs maximal strength or 1 repetition maximum (RM) in untrained subjects during half-squat exercise. One hundred and five physically active young subjects (87 men and 18 women) performed a submaximal and a maximal load test during half-squat exercises on a Smith machine. In the submaximal test, subjects completed 3 repetitions with a load equivalent to body weight. The velocity and power of barbell displacement were recorded during the upward movement from 90° of knee flexion. All repetitions were performed at maximum velocity. In a subsequent 1–2RM test, the 1RM for the exercise was calculated. The variablesʼ load and mean velocity (Vmean) were used to construct an adjusted 1RM prediction model, which was capable of estimating the 1RM with an accuracy of 58% (Fexp = 72.82; 2; 102 df; p ≤ 0.001). Our results indicate a good correlation between the mean displacement velocity of a load equivalent to body weight and 1RM. This relationship enables a safe and fast estimation of 1RM values in half-squat exercise (1RM = −61.93 + 121.92·Vmean + 1.74·load) and provides valuable information to untrained subjects who are starting resistance training programs.
Background
Nail changes in patients with congenital epidermolysis bullosa (EB) are caused by abnormalities of the nail matrix and bed secondary to pathogenic alterations of the dermoepidermal ...junction. Even though ungual alterations are extremely frequent in these patients, there are scarce studies about their frequency and/or association with subtypes or clinical course of EB.
Objectives
To systematically review nail abnormalities in patients with EB reported in the literature.
Methods
We searched all published articles in electronic databases until June 2020 reporting patients with EB with detailed descriptions of malformed/diseased nails using specific terms and inclusion/exclusion criteria. Clinical data were extracted by two independent authors. Descriptive statistics were used.
Results
We included 36 articles reporting 74 individual patients with a mean age of 28.23 years: 29 (39.2%) had dominant dystrophic EB, 27 (36.4%) had junctional EB, 8 (10.8%) had EB simplex, 6 (8.1%) had Kindler syndrome and 4 (5.4%) had recessive dystrophic EB. The most common abnormalities were dystrophic nails (48.6%), anonychia (43.2%) and pachyonychia (40.5%). Anonychia was considered the most severe abnormality and was reported more frequently in patients with junctional (62.9%) and recessive dystrophic EB (50%). Multiple organ involvement was present in 52.7% of patients. Patients with severe junctional epidermolysis bullosa and recessive dominant epidermolysis bullosa presented anonychia since birth.
Conclusions
In this summary of nail abnormalities in patients with EB, anonychia was more frequent in patients with severe EB subtypes and multiple organ involvement. Further prospective studies are required to understand the associations between nail abnormalities in specific EB subtypes and/or patient outcomes.
Nail changes in patient with congenital epidermolysis bullosa (EB) are a result of abnormalities of the nail matrix and bed secondary to pathogenic alterations of the dermoepidermal junction. They are extremely frequent, but there are scarce studies about their frequency and/or association with subtypes or clinical course. This systematic review of the literature found that all patients with EB have nail abnormalities, regardless of subtype. The most common ungual alterations were dystrophic nails (48.6%), anonychia (43.2%) and pachyonychia (40.5%). Anonychia was more frequent in patients with multiple organ involvement, and loss of multiple nails since birth or early life was reported in patients with severe subtypes.
