The HAWC observatory is an air-shower detector, which is designed to study both astrophysical gamma-rays in the TeV region and galactic cosmic rays in the energy interval from 1 TeV to 1 PeV. This ...energy regime is interesting for cosmic ray research, since indirect observations overlap with direct measurements, which offers the opportunity for cross calibration and studies of experimental systematic errors in both techniques. One quantity that could help for this purpose is the all-particle energy spectrum of cosmic rays. In this work, we present an update of HAWC measurements on the total cosmic-ray energy spectrum between 10 TeV and 1 PeV. The spectrum was obtained from an unfolding analysis of almost two years of HAWC’s data, which was collected from January, 2018 to December, 2019. For the energy estimation, we employed the high-energy hadronic interaction model QGSJET-II-04. As in a previous work of HAWC, published in 2017, we observed the presence of a knee-like feature in the region of tens of TeV.
Weed coexistence with an agricultural crop can negatively affect its growth, development, and yield. From this perspective, this study aimed to evaluate weed management strategies and their effect on ...the agronomic parameters of cowpea cultivation in direct (SPD) and conventional (SPC) planting systems. The experiment was set up in a completely randomized block design with a split-plot arrangement with four replications. The plots received a source of variation referring to the planting systems (direct and conventional planting), and the subplots corresponded to ten weed management strategies (manual hoeing 18 days after planting (DAP); at 36 DAP; at 54 DAP; at 18 and 36 DAP; at 18 and 54 DAP; at 18 and 72 DAP; at 36 and 54 DAP; at 36-72 DAP; at 18, 36, and 54 DAP; and a control with no hoeing). Density and dry mass evaluations of the cowpea plants were performed at harvest (72 DAP) by determining the number of pods per plant, pod length, number of grains per pod, 1000-grain mass, and yield. A total of 28 species distributed in 12 botanical families were identified in the two cultivation systems. The family
showed the highest frequency, with 25% of the species identified. At the end of the assay, treatment 20 had the highest positive influence and provided significant quantitative gains to the complex of traits related to cowpea production (SPD and hoeing at 18, 36, and 54 DAP). UPGMA cluster analysis and canonical discriminant analysis were performed and allowed a better classification of the evaluated treatments. It was observed that the first two canonical variables explained 90.8% of the total variance contained in the original variables. The use of SPD with weeding at 18, 36, and 54 days after planting provides greater weed control and significant quantitative gains for the complex of characteristics related to cowpea production. The results underscore the importance of choosing the correct cropping system and implementing effective weeding practices to optimize weed control and improve crop performance.
Glioblastoma (GB) is the most aggressive form of glioma and is characterized by poor prognosis and high recurrence despite intensive clinical interventions. To retrieve the key factors underlying the ...high malignancy of GB with potential diagnosis utility, we combined the analysis of The Cancer Gene Atlas and the REMBRANDT datasets plus a molecular examination of our own collection of surgical tumor resections. We determined a net reduction in the levels of the non-canonical histone H3 variant H3.3 in GB compared to lower-grade astrocytomas and oligodendrogliomas with a concomitant increase in the levels of the canonical histone H3 variants H3.1/H3.2. This increase can be potentially useful in the clinical diagnosis of high-grade gliomas, as evidenced by an immunohistochemistry screening of our cohort and can be at least partially explained by the induction of multiple histone genes encoding these canonical forms. Moreover, GBs showing low bulk levels of the H3.1/H3.2 proteins were more transcriptionally similar to low-grade gliomas than GBs showing high levels of H3.1/H3.2. In conclusion, this study identifies an imbalanced ratio between the H3 variants associated with glioma malignancy and molecular patterns relevant to the biology of gliomas, and proposes the examination of the H3.3 and H3.1/H3.2 levels to further refine diagnosis of low- and high-grade gliomas in future studies.
Acute and subacute administration of zinc exert neuroprotective effects in hypoxia-ischemia animal models; yet the effect of chronic administration of zinc still remains unknown. We addressed this ...issue by injecting zinc at a tolerable dose (0.5 mg/kg weight, i.p.) for 14 days before common carotid artery occlusion (CCAO) in a rat. After CCAO, the level of zinc was measured by atomic absorption spectrophotometry, nitrites were determined by Griess method, lipoperoxidation was measured by Gerard-Monnier assay, and mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors was measured by qRT-PCR, whereas nitrotyrosine, chemokines, and their receptors were assessed by ELISA and histopathological changes in the temporoparietal cortex-hippocampus at different time points. Long-term memory was evaluated using Morris water maze. Following CCAO, a significant increase in nitrosative stress, inflammatory chemokines/receptors, and cell death was observed after 8 h, and a 2.5-fold increase in zinc levels was detected after 7 days. Although CXCL12 and FGF2 protein levels were significantly increased, the long-term memory was impaired 12 days after reperfusion in the Zn+CCAO group. Our data suggest that the chronic administration of zinc at tolerable doses causes nitrosative stress, toxic zinc accumulation, and neuroinflammation, which might account for the neuronal death and cerebral dysfunction after CCAO.
There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly ...consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzoapyrene (BaP) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with BaP (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with BaP and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzoapyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with BaP showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with BaP and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second part of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the BaP increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60%) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5% in relation to the animals administered with the BaP. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against BaP is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition.
La información actualizada sobre la representatividad geográfica y taxonómica de los ejemplares depositados en 28 colecciones mastozoológicas mexicanas y la disponible en las bases de datos Mammal ...Networked Information System (MaNIS), y de la Unidad de Informática para la Biodiversidad (UNIBIO) se recopiló con el fin de analizar y comparar el estado actual de los ejemplares de mamíferos mexicanos depositados en colecciones de Norteamérica. Se documentaron un total de 421,466 ejemplares de mamíferos depositados en 120 colecciones mastozoológicas, de los cuales 233,116 ejemplares se encuentran en colecciones de Estados Unidos de América y Canadá, y 188,350 ejemplares en colecciones de México. Las colecciones con el mayor número de ejemplares de mamíferos de México son la Colección Nacional de Mamíferos (CNMA 46,000), la Universidad de Kansas (KU 44,301), y la Escuela Nacional de Ciencias Biológicas (ENCB 43,985). En colecciones mexicanas, los estados del norte de México están escasamente representados en número de ejemplares (< 3,000), y en las colecciones extranjeras, los estados del centro de México están pobremente representados. Los estados mexicanos mejor representados por número de ejemplares (> 10,000) son Baja California Sur, Chiapas, Oaxaca, Puebla y Veracruz. Los órdenes mejor representados por el número de ejemplares son Rodentia y Chiroptera (234,978 y 120,956, respectivamente). Los estados mexicanos con menor extensión territorial tienen la mayor densidad de ejemplares colectados (ejemplares/km²): Distrito Federal (3.2), Tlaxcala (1.4), Colima (1.06) y Morelos (0.9). Se espera contar con una línea base que permita establecer estrategias adecuadas para completar el inventario nacional de los mamíferos mexicanos, y lograr que este patrimonio natural este adecuadamente representado en colecciones de México.
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