This objective of this work was to assess the knowledge of students in public schools about rabies and leishmaniasis, through educational interventions. A total of 628 questionnaires were applied in ...public students from Apodi, Felipe Guerra and Severiano Melo. The questionnaires were administered before and after the educational interventions in the form of lectures. The data were discussed through a descriptive analysis. Before the presentation, 53.9% of students claimed to know the transmission mode of leishmaniasis and after, this percentage increased to 92.2%. However, before the lectures, only 6.0% of respondents associated the transmission to sandflies, and after, this percentage increased to 75.7%. For rabies, before the lectures, 63.7% of students reported knowing how rabies is transmitted and, after the lectures, 92.8% said how it was the transmission. Concerning the transmission, before the presentations, 68.3% reported that it was through bite or injury of animals and, after the lectures, the index increased to 80.4%. In conclusion, the student´s perception of public education on rabies and leishmaniasis was higher in all aspects addressed after educational intervention compared to the same questions about the knowledge of the disease before the intervention. It also concludes that the perception of students for rabies is greater than for leishmaniasis.
The goals of the study were to verify the presence of coliforms in frozen Basa fish fillets market in different cities in Mato Grosso State, Brazil and identify if they complied with the standards ...established by the Brazilian Food Legislation. Twenty-five samples were collected at supermarkets in eight cities in Mato Grosso State. The microbiological analyses were performed using the coagulase-positive Staphylococci count; screening for Salmonella sp., enumeration of total coliforms, thermotolerant and Escherichia coli. In addition, it proceeded to parasitological analysis by parasitic morphological structures identification. Coagulase-positive Staphylococci strains were isolated in two samples (8%) with 3,8 x 10 4 and 1,4 x 10 5 CFU/g. In one sample (4%), the values obtained were 23 MPN/ g for total coliforms count, 4 MPN/ g for thermotolerant coliforms and 4 MPN/ g for E. coli. Salmonella spp. strains were not isolated. Parasitic structures were not observed. Frozen Panga fish fillets demonstrated low levels of coliform contamination; indicating that it is safe for human consumption considering that 23 (92%) of the analyzed samples were within the standards established by the current Brazilian Food Legislation.
The goals of the study were to verify the presence of coliforms in frozen Basa fish fillets market in different cities in Mato Grosso State, Brazil and identify if they complied with the standards ...established by the Brazilian Food Legislation. Twenty-five samples were collected at supermarkets in eight cities in Mato Grosso State. The microbiological analyses were performed using the coagulase-positive Staphylococci count; screening for Salmonella sp., enumeration of total coliforms, thermotolerant and Escherichia coli. In addition, it proceeded to parasitological analysis by parasitic morphological structures identification. Coagulase-positive Staphylococci strains were isolated in two samples (8%) with 3,8 x 10 4 and 1,4 x 10 5 CFU/g. In one sample (4%), the values obtained were 23 MPN/ g for total coliforms count, 4 MPN/ g for thermotolerant coliforms and 4 MPN/ g for E. coli. Salmonella spp. strains were not isolated. Parasitic structures were not observed. Frozen Panga fish fillets demonstrated low levels of coliform contamination; indicating that it is safe for human consumption considering that 23 (92%) of the analyzed samples were within the standards established by the current Brazilian Food Legislation.
Antibodies, a prominent class of approved biologics, play a crucial role in detecting foreign antigens. The effectiveness of antigen neutralisation and elimination hinges upon the strength, ...sensitivity, and specificity of the paratope-epitope interaction, which demands resource-intensive experimental techniques for characterisation. In recent years, artificial intelligence and machine learning methods have made significant strides, revolutionising the prediction of protein structures and their complexes. The past decade has also witnessed the evolution of computational approaches aiming to support immunotherapy design. This review focuses on the progress of machine learning-based tools and their frameworks in the domain of B-cell immunotherapy design, encompassing linear and conformational epitope prediction, paratope prediction, and antibody design. We mapped the most commonly used data sources, evaluation metrics, and method availability and thoroughly assessed their significance and limitations, discussing the main challenges ahead.
Abstract
This study investigated the progressive effects of HgCl
2
in the testis and sperm of the tropical fish tuvira
G
ymnotus carapo
L. exposed to increasing Hg concentrations (5–30 μ
m
) and ...increasing exposure times (24–96 h). Histopathology and metal concentrations in the testis were observed. Hg concentrations in the testis reached 5.1 and 5.2 μg g
−1
in fish exposed to 20 and 30 μ
m
of Hg, respectively. Hg effects on testicular tissue were observed even at low Hg concentrations, with no alterations in gonadosomatic index. However, the quantitative analysis of the induced alterations (lesion index) demonstrated that the Hg effects in testis became more severe after exposure to higher concentrations (20 and 30 μ
m
) and during longer exposure (72 and 96 h), probably leading to partial or total loss of the organ function. Hg exposure (20 μ
m
) also affected sperm count and altered sperm morphology. This study showed that HgCl
2
caused progressive damage to testicular tissue, reduced sperm count and altered sperm morphology. These results are important in establishing a direct correlation between Hg accumulation and severity of lesions.
A satellite DNA sequence of Parodon hilarii (named pPh2004) was isolated, cloned and sequenced. This satellite DNA is composed of 200bp, 60% AT rich. In situ hybridization (FISH) results revealed ...that the satellite DNA pPh2004 is located in the terminal regions of several chromosomes, forming highly evident blocks in some and punctual marks in others. The comparison between the FISH and C-banding results showed that the location of this satellite DNA coincides with that of most terminal heterochromatins. However, some regions are only marked by FISH whereas other regions are only marked by C-banding. The possible existence of more than one satellite DNA family could explain these partial differences. The in situ hybridization with the satellite DNA and the G- and C-bandings confirmed the presence of a sex chromosome system of the ZZ/ZW type in P. hilarii, as well as the correct identification of the Z chromosome in the karyotype. This chromosome displays a segment of terminal heterochromatin in the long arm, similar to the segment observed in the short arm of the W chromosome, also showing a G-banding pattern similar to that of the short arm and part of the long arm of the W chromosome. A hypothesis on the origin of the W chromosome from an ancestral chromosome similar to the Z chromosome is presented.
Cytogenetic studies were conducted on 154 specimens of Astyanax scabripinnis collected at three localities in the Campos do Jordão region (State of São Paulo, Brazil). The C-banding pattern suggested ...that the metacentric B chromosome found in most of the specimens is an isochromosome derived from chromosome 24, the only chromosome in the standard complement that carries interstitial C-bands similar to those present in each arm of the B chromosome. The sex ratio was biased toward females in the Córrego das Pedras and Ribeirão do Casquilho streams and toward males in the Ribeirão das Perdizes stream. In all three populations analyzed, the B chromosome was more frequent in females than in males. In the most exhaustively sampled population (those from Córrego das Pedras), there was a highly significant association between B-chromosome frequency and sex-ratio distortion, with a disproportionately high number of males without B chromosomes and females with one B chromosome.
Heart failure (HF) is known to lead to skeletal muscle atrophy and dysfunction. However, intracellular mechanisms underlying HF-induced myopathy are not fully understood. We hypothesized that HF ...would increase oxidative stress and ubiquitin-proteasome system (UPS) activation in skeletal muscle of sympathetic hyperactivity mouse model. We also tested the hypothesis that aerobic exercise training (AET) would reestablish UPS activation in mice and human HF.
Time-course evaluation of plantaris muscle cross-sectional area, lipid hydroperoxidation, protein carbonylation and chymotrypsin-like proteasome activity was performed in a mouse model of sympathetic hyperactivity-induced HF. At the 7(th) month of age, HF mice displayed skeletal muscle atrophy, increased oxidative stress and UPS overactivation. Moderate-intensity AET restored lipid hydroperoxides and carbonylated protein levels paralleled by reduced E3 ligases mRNA levels, and reestablished chymotrypsin-like proteasome activity and plantaris trophicity. In human HF (patients randomized to sedentary or moderate-intensity AET protocol), skeletal muscle chymotrypsin-like proteasome activity was also increased and AET restored it to healthy control subjects' levels.
Collectively, our data provide evidence that AET effectively counteracts redox imbalance and UPS overactivation, preventing skeletal myopathy and exercise intolerance in sympathetic hyperactivity-induced HF in mice. Of particular interest, AET attenuates skeletal muscle proteasome activity paralleled by improved aerobic capacity in HF patients, which is not achieved by drug treatment itself. Altogether these findings strengthen the clinical relevance of AET in the treatment of HF.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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