PurposeThe purpose of this paper is to examine the relationship between digital skills and employment and in this way accentuate importance of policy interventions for improving digital ...literacy.Design/methodology/approachUsing Eurostat data, this paper demonstrates statistically significant correlation between digital skills and employment rates in EU.FindingsThis implies why it is so important for governments and employers to seek, propose and implement new strategies to promote digital inclusion, literacy and the training not only for new ICT professionals but also for the whole workforce.Social implicationsDigital skills, particularly digital literacy, are an important factor for socio-economic development of the society and employability of the labour force. Without adequate digital literacy, it is not possible to participate in the economy and the digital society, particularly having in mind the digital transformation that the world of work is experiencing in the content and the organisation. Beyond the work environment, it also affects the way people live and communicate.Originality/valueIn this paper, the importance and definition of digital literacy are elaborated, explaining the measurement of digital literacy and skills, as well as the relation between digital literacy and employability.
The evolution of modern healthcare underscores the crucial question of societal priorities in development. When distributing finite healthcare resources, decision-makers must weigh numerous factors ...and navigate a range of sometimes conflicting criteria, including equity, access, fairness, and effectiveness. This chapter aims to survey the social implications of digital literacy as a pivotal element for the optimal advancement of P5 medicine, with the objective of enhancing healthcare services. The untapped potential of telemedicine and other emerging P5 technologies remains substantial in terms of their integration into routine medical practice. Adequate digital literacy among both healthcare providers and users stands as a vital prerequisite for the successful adoption and further evolution of these innovations. Coupled with improved health literacy, these factors collectively should contribute to the provision of superior healthcare services and consequently to the overall well-being of the populace.
This research explores the interconnectedness between tourism infrastructure, recreational facilities and tourism development. It analyses their importance in, and compliance with the current phase ...of tourism development in the destination (TALC). Attention has been given to the tourist board managers' perception of infrastructural management and key limitation for their involvement in the management process. Finally, the role of the private sector in the development of infrastructure and facilities in destination has been explored. The semi-structured questioner has been repeatedly sent to 312 tourist board managers in Croatia, leading Southern Mediterranean destination. The research applies qualitative and quantitative analysis. There is a significant correlation between TALC and number of arrivals, overnights, the current state of the infrastructure and facilities. Findings suggest growing demand and expectations regarding infrastructure and facilities in the examined destination can be related to a destination position in TALC. The compliance level between the stage of the tourism development and state of the infrastructure and facilities varies especially between destinations in initial and maturing phases of tourism development. The destinations position in TALC is correlated with the importance of specific types of infrastructure and facilities for a specific destination. Due to mostly financial limitations, managers are not willing to take responsibility for the development of tourism infrastructure. Their expectations regarding private sector involvement vary, considering the type of infrastructure, facilities and destinations position in TALC. Research provides supply-side perspective and new insights into the infrastructural development - TALC relation, and delivers tourist board managers attitudes toward the private sector involvement.
The concept of a last universal common ancestor of all cells (LUCA, or the progenote) is central to the study of early evolution and life's origin, yet information about how and where LUCA lived is ...lacking. We investigated all clusters and phylogenetic trees for 6.1 million protein coding genes from sequenced prokaryotic genomes in order to reconstruct the microbial ecology of LUCA. Among 286,514 protein clusters, we identified 355 protein families (∼0.1%) that trace to LUCA by phylogenetic criteria. Because these proteins are not universally distributed, they can shed light on LUCA's physiology. Their functions, properties and prosthetic groups depict LUCA as anaerobic, CO2-fixing, H2-dependent with a Wood-Ljungdahl pathway, N2-fixing and thermophilic. LUCA's biochemistry was replete with FeS clusters and radical reaction mechanisms. Its cofactors reveal dependence upon transition metals, flavins, S-adenosyl methionine, coenzyme A, ferredoxin, molybdopterin, corrins and selenium. Its genetic code required nucleoside modifications and S-adenosyl methionine-dependent methylations. The 355 phylogenies identify clostridia and methanogens, whose modern lifestyles resemble that of LUCA, as basal among their respective domains. LUCA inhabited a geochemically active environment rich in H2, CO2 and iron. The data support the theory of an autotrophic origin of life involving the Wood-Ljungdahl pathway in a hydrothermal setting.
The ALICE Experiment at CERN’s Large Hadron Collider (LHC) has undergone a major upgrade during LHC Long Shutdown 2 in 2019-2021, which includes a new computing system called O 2 (Online-Offline). To ...ensure the efficient operation of the upgraded experiment and of its newly designed computing system, a reliable, high performance, full-featured experiment control system has also been developed and deployed at LHC Point 2. The ALICE Experiment Control System (AliECS) is a microservices-oriented system based on state-of-the-art cluster management technologies that emerged recently in the distributed and high-performance computing ecosystem. It is designed, developed and maintained as a comprehensive solution and single entry point for control of experiment data acquisition (up to 3.5 TB/s) and processing. This communication describes the AliECS architecture by providing an in-depth overview of the system’s components, interfaces, features, and design elements, as well as its performance. It also reports on the experience with AliECS during the first year of ALICE Run 3 data taking with LHC beam, including integration and operational challenges, and lessons learned from real-world use.
Chemical Antiquity in Metabolism Mrnjavac, Natalia; Schwander, Loraine; Brabender, Max ...
Accounts of chemical research,
07/2024
Journal Article
Recenzirano
Odprti dostop
ConspectusLife is an exergonic chemical reaction. The same was true when the very first cells emerged at life's origin. In order to live, all cells need a source of carbon, energy, and electrons to ...drive their overall reaction network (metabolism). In most cells, these are separate pathways. There is only one biochemical pathway that serves all three needs simultaneously: the acetyl-CoA pathway of CO2 fixation. In the acetyl-CoA pathway, electrons from H2 reduce CO2 to pyruvate for carbon supply, while methane or acetate synthesis are coupled to energy conservation as ATP. This simplicity and thermodynamic favorability prompted Georg Fuchs and Erhard Stupperich to propose in 1985 that the acetyl-CoA pathway might mark the origin of metabolism, at the same time that Steve Ragsdale and Harland Wood were uncovering catalytic roles for Fe, Co, and Ni in the enzymes of the pathway. Subsequent work has provided strong support for those proposals.In the presence of Fe, Co, and Ni in their native metallic state as catalysts, aqueous H2 and CO2 react specifically to formate, acetate, methane, and pyruvate overnight at 100 °C. These metals (and their alloys) thus replace the function of over 120 enzymes required for the conversion of H2 and CO2 to pyruvate via the pathway and its cofactors, an unprecedented set of findings in the study of biochemical evolution. The reactions require alkaline conditions, which promote hydrogen oxidation by proton removal and are naturally generated in serpentinizing (H2-producing) hydrothermal vents. Serpentinizing hydrothermal vents furthermore produce natural deposits of native Fe, Co, Ni, and their alloys. These are precisely the metals that reduce CO2 with H2 in the laboratory; they are also the metals found at the active sites of enzymes in the acetyl-CoA pathway. Iron, cobalt and nickel are relicts of the environments in which metabolism arose, environments that still harbor ancient methane- and acetate-producing autotrophs today. This convergence indicates bedrock-level antiquity for the acetyl-CoA pathway. In acetogens and methanogens growing on H2 as reductant, the acetyl-CoA pathway requires flavin-based electron bifurcation as a source of reduced ferredoxin (a 4Fe4S cluster-containing protein) in order to function. Recent findings show that H2 can reduce the 4Fe4S clusters of ferredoxin in the presence of native iron, uncovering an evolutionary precursor of flavin-based electron bifurcation and suggesting an origin of FeS-dependent electron transfer in proteins. Traditionally discussed as catalysts in early evolution, the most common function of FeS clusters in metabolism is one-electron transfer, also in radical SAM enzymes, a large and ancient enzyme family. The cofactors and active sites in enzymes of the acetyl-CoA pathway uncover chemical antiquity in metabolism involving metals, methyl groups, methyl transfer reactions, cobamides, pterins, GTP, S-adenosylmethionine, radical SAM enzymes, and carbon-metal bonds. The reaction sequence from H2 and CO2 to pyruvate on naturally deposited native metals is maximally simple. It requires neither nitrogen, sulfur, phosphorus, RNA, ion gradients, nor light. Solid-state metal catalysts tether the origin of metabolism to a H2-producing, serpentinizing hydrothermal vent.ConspectusLife is an exergonic chemical reaction. The same was true when the very first cells emerged at life's origin. In order to live, all cells need a source of carbon, energy, and electrons to drive their overall reaction network (metabolism). In most cells, these are separate pathways. There is only one biochemical pathway that serves all three needs simultaneously: the acetyl-CoA pathway of CO2 fixation. In the acetyl-CoA pathway, electrons from H2 reduce CO2 to pyruvate for carbon supply, while methane or acetate synthesis are coupled to energy conservation as ATP. This simplicity and thermodynamic favorability prompted Georg Fuchs and Erhard Stupperich to propose in 1985 that the acetyl-CoA pathway might mark the origin of metabolism, at the same time that Steve Ragsdale and Harland Wood were uncovering catalytic roles for Fe, Co, and Ni in the enzymes of the pathway. Subsequent work has provided strong support for those proposals.In the presence of Fe, Co, and Ni in their native metallic state as catalysts, aqueous H2 and CO2 react specifically to formate, acetate, methane, and pyruvate overnight at 100 °C. These metals (and their alloys) thus replace the function of over 120 enzymes required for the conversion of H2 and CO2 to pyruvate via the pathway and its cofactors, an unprecedented set of findings in the study of biochemical evolution. The reactions require alkaline conditions, which promote hydrogen oxidation by proton removal and are naturally generated in serpentinizing (H2-producing) hydrothermal vents. Serpentinizing hydrothermal vents furthermore produce natural deposits of native Fe, Co, Ni, and their alloys. These are precisely the metals that reduce CO2 with H2 in the laboratory; they are also the metals found at the active sites of enzymes in the acetyl-CoA pathway. Iron, cobalt and nickel are relicts of the environments in which metabolism arose, environments that still harbor ancient methane- and acetate-producing autotrophs today. This convergence indicates bedrock-level antiquity for the acetyl-CoA pathway. In acetogens and methanogens growing on H2 as reductant, the acetyl-CoA pathway requires flavin-based electron bifurcation as a source of reduced ferredoxin (a 4Fe4S cluster-containing protein) in order to function. Recent findings show that H2 can reduce the 4Fe4S clusters of ferredoxin in the presence of native iron, uncovering an evolutionary precursor of flavin-based electron bifurcation and suggesting an origin of FeS-dependent electron transfer in proteins. Traditionally discussed as catalysts in early evolution, the most common function of FeS clusters in metabolism is one-electron transfer, also in radical SAM enzymes, a large and ancient enzyme family. The cofactors and active sites in enzymes of the acetyl-CoA pathway uncover chemical antiquity in metabolism involving metals, methyl groups, methyl transfer reactions, cobamides, pterins, GTP, S-adenosylmethionine, radical SAM enzymes, and carbon-metal bonds. The reaction sequence from H2 and CO2 to pyruvate on naturally deposited native metals is maximally simple. It requires neither nitrogen, sulfur, phosphorus, RNA, ion gradients, nor light. Solid-state metal catalysts tether the origin of metabolism to a H2-producing, serpentinizing hydrothermal vent.
Serpentinization in hydrothermal vents is central to some autotrophic theories for the origin of life because it generates compartments, reductants, catalysts and gradients. During the process of ...serpentinization, water circulates through hydrothermal systems in the crust where it oxidizes Fe (II) in ultramafic minerals to generate Fe (III) minerals and H
2
. Molecular hydrogen can, in turn, serve as a freely diffusible source of electrons for the reduction of CO
2
to organic compounds, provided that suitable catalysts are present. Using catalysts that are naturally synthesized in hydrothermal vents during serpentinization H
2
reduces CO
2
to formate, acetate, pyruvate, and methane. These compounds represent the backbone of microbial carbon and energy metabolism in acetogens and methanogens, strictly anaerobic chemolithoautotrophs that use the acetyl-CoA pathway of CO
2
fixation and that inhabit serpentinizing environments today. Serpentinization generates reduced carbon, nitrogen and — as newer findings suggest — reduced phosphorous compounds that were likely conducive to the origins process. In addition, it gives rise to inorganic microcompartments and proton gradients of the right polarity and of sufficient magnitude to support chemiosmotic ATP synthesis by the rotor-stator ATP synthase. This would help to explain why the principle of chemiosmotic energy harnessing is more conserved (older) than the machinery to generate ion gradients via pumping coupled to exergonic chemical reactions, which in the case of acetogens and methanogens involve H
2
-dependent CO
2
reduction. Serpentinizing systems exist in terrestrial and deep ocean environments. On the early Earth they were probably more abundant than today. There is evidence that serpentinization once occurred on Mars and is likely still occurring on Saturn’s icy moon Enceladus, providing a perspective on serpentinization as a source of reductants, catalysts and chemical disequilibrium for life on other worlds.
Long-term unemployment (when a person is jobless for more than 12 months) can have serious negative consequences for the individual, society and its economic system. People who are unemployed for a ...long-term period find it more difficult to be employed as time goes by. Long-term unemployed persons often have limited employability, due to their low level of educational attainment, loss of skills and the substantial expenditures that are necessary to restore these skills. They also face significantly lower earnings and restricted career prospects. The participation rate of the long-term unemployed in employment programmes is usually very low. The article considers the significance of long-term unemployment in Croatia and measures for its reduction.
The ALICE Experiment at CERN LHC (Large Hadron Collider) is under preparation for a major upgrade that is scheduled to be deployed during Long Shutdown 2 in 2019-2020 and that includes new computing ...systems, called O2 (Online-Offine). To ensure the efficient operation of the upgraded experiment along with its newly designed computing system, a reliable, high performance and automated control system will be developed with the goal of managingthe lifetime of all the O2 processes, and of handling the various phases of the data taking activity by interacting with the detectors, the trigger system and the LHC. The ALICE O2 control system will be a distributed systembased on state of the art cluster management and microservices which have recently emerged in the distributed computing ecosystem. Such technologies weren’t available during the design and development of the original LHC computing systems, and their use will allow the ALICE collaboration to benefit from a vibrant and innovatingopen source community. This paper illustrates the O2 control system architecture. It evaluates several olutionsthat were considered during an initial prototyping phase and provides a rationale for the choices made. It also provides an in-depth overview of the components, features and design elements of the actual system.
ALICE (A Large Ion Collider Experiment) has undertaken a major upgrade during the LHC Long Shutdown 2. The increase in the detector data rates led to a hundredfold increase in the input raw data, up ...to 3.5 TB/s. To cope with it, a new common Online and Offline computing system, called O 2 , has been developed and put in production. The O 2 /FLP (First Level Processor) system, successor of the ALICE DAQ system, implements the critical functions of detector readout, data quality control and operational services running in the CR1 data centre at the experimental site. Data from the 15 ALICE subdetectors are read out via 8000 optical links by 500 custom PCIe cards hosted in 200 nodes. It addresses novel challenges such as the continuous readout of the TPC detector while keeping compatibility with legacy detector front-end electronics. This paper discusses the final architecture and design of the O 2 /FLP system and provides an overview of all its components, both hardware and software. It presents the selection process for the FLP nodes, the different commissioning steps and the main accomplishments so far. It will conclude with the challenges that lie ahead and how they will be addressed.
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