The novel coronavirus SARS-CoV-2 resulted in a significant worldwide health emergency known as the COVID-19 pandemic. This crisis has been marked by the widespread of various variants, with certain ...ones causing notable apprehension. In this study, we harnessed computational techniques to scrutinize these Variants of Concern (VOCs), including various Omicron subvariants. Our approach involved the use of protein structure prediction algorithms and molecular docking techniques, we have investigated the effects of mutations within the Receptor Binding Domain (RBD) of SARS-CoV-2 and how these mutations influence its interactions with the human angiotensin-converting enzyme 2 (hACE-2) receptor. Further we have predicted the structural alterations in the RBD of naturally occurring SARS-CoV-2 variants using the tr-Rosetta algorithm. Subsequent docking and binding analysis employing HADDOCK and PRODIGY illuminated crucial interactions occurring at the Receptor-Binding Motif (RBM). Our findings revealed a hierarchy of increased binding affinity between the human ACE2 receptor and the various RBDs, in the order of wild type (Wuhan-strain) < Beta < Alpha < Gamma < Omicron-B.1.1.529 < Delta < Omicron-BA.2.12.1 < Omicron-BA.5.2.1 < Omicron-BA.1.1. Notably, Omicron-BA.1.1 demonstrated the highest binding affinity of -17.4 kcal mol
to the hACE2 receptor when compared to all the mutant complexes. Additionally, our examination indicated that mutations occurring in active residues of the Receptor Binding Domain (RBD) consistently improved the binding affinity and intermolecular interactions in all mutant complexes. Analysis of the differences among variants has laid a foundation for the structure-based drug design targeting the RBD region of SARS-CoV-2.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
A reconfigurable gain circuit is proposed for an LCLC resonant converter with bidirectional capability considering wide varying redox flow battery source. A suitable hybrid control scheme for LCLC ...resonant converter with secondary synchronous rectifier is proposed in this work. The proposed reconfigurable circuit enables to configure secondary bridge as an active voltage doubler, full-bridge circuit during forward power transfer mode and reverse power transfer mode, respectively. The reconfigurability helps significantly to design a transformer with lower secondary turns and achieve desired high gain. The reduced transformer secondary turns result in reduced transformer parasitics. The presence of LCLC resonant tank helps to provide the additional gain; the hybrid control scheme ensures zero-voltage switching turn- on for the primary, and secondary synchronous mosfets throughout the operating range. The proposed converter is analysed using fundamental harmonic approximation. The proposed reconfigurable gain circuit and hybrid control scheme is verified experimentally for an 800 V/1 kW hardware prototype fed from 24 to 54 V input.
The research was conducted during August to November, 2020 at the Department of Plant Biotechnology and Dryland Farming Unit, University of Agricultural Sciences (UAS), Gandhi Krishi Vigyana Kendra ...(GKVK), Bengaluru, Karnataka, India to validate the identified SSR markers associated with resistance to yellow mosaic diseases of cowpea using different genotypes. 120 germplasm lines cowpea were used for the study including two check varieties (IC-202781 and V-585). Disease scoring scale by Diwakar and Mali (1976) was used for the disease scoring. Among 120 genotypes, 52 genotypes with moderately resistance, 28 genotypes with no disease symptoms, 15 genotypes with resistance, 14 genotypes with moderately susceptible, 11 genotypes with susceptible and no highly susceptible genotypes were observed. These germplasms were screened using four simple sequences repeat markers (SSR) specific to Bean common mosaic virus and four SSR markers specific to cowpea yellow mosaic virus were selected from the existing databases. Out of the four SSR marker linked to BCMV tested for validation, only three markers (M15, M80, and Y 96) gave the desired amplification. Single marker analysis (SMA) revealed that out of four SSR markers, MA15 marker explains 3.96% of phenotypic variation, among 4 SSR markers linked to CYMV, only two markers (VM1 and AG1/AF48383) gave the desired amplification. SMA revealed that out of two SSR markers, AG1/AF48383 marker explains 3.80% of phenotypic variation. The resistant genotypes identified in this investigation can be used in future breeding programmes for introgression of disease resistant traits in important competitive varieties in the market.
Over 380 host plant species have been known to develop leaf spots as a result of the fungus Alternaria alternata. It is an aspiring pathogen that affects a variety of hosts and causes rots, blights, ...and leaf spots on different plant sections. In this investigation, the lipopeptides from the B. subtilis strains T3, T4, T5, and T6 were evaluated for their antifungal activities. In the genomic DNA, iturin, surfactin, and fengycin genes were found recovered from B. subtilis bacterium by PCR amplification. From different B. subtilis strains, antifungal Lipopeptides were extracted, identified by HPLC, and quantified with values for T3 (24 g/ml), T4 (32 g/ml), T5 (28 g/ml), and T6 (18 g/ml). To test the antifungal activity, the isolated lipopeptides from the B. subtilis T3, T4, T5, and T6 strains were applied to Alternaria alternata at a concentration of 10 g/ml. Lipopeptides were found to suppress Alternaria alternata at rates of T3 (75.14%), T4 (75.93%), T5 (80.40%), and T6 (85.88%). The T6 strain outperformed the other three by having the highest antifungal activity against Alternaria alternata (85.88%).
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Synchronous rectifier schemes for the resonant DCDC converter significantly improve the power conversion efficiency. The predictive synchronous rectification scheme for an LCLC resonant converter for ...a battery charging and telecom application is proposed in this work. During converter operation, the resonant capacitor voltage is sensed through an analog to digital converter, and based on the discretized time domain equations, the turn-on and turn-off instances are precisely computed using computation and synchronous MOSFET on time predictor block for varying switching frequency of operation. The proposed predictive technique eliminates the need for current sensors, reducing overall system cost and conduction loss associated with it. The proposed predictive synchronous rectification method is tested in a 1 kW hardware prototype for varying line and load conditions. The proposed control scheme is able to track turn-on and turn-off instances precisely with a low timing error of 2 % to 3 % of the synchronous MOSFET ON time.
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in ...t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay of the epigenome and transcription factors prevents apoptosis in t(8;21) AML cells.
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•Global analysis of AML1-ETO (AE) in two patient blasts•The AML1-ETO complex consists of hematopoietic, chromatin, and splicing regulators•ERG or RUNX1 knockdown upregulates AML1-ETO expression•AML1-ETO overexpression in differentiated iPSCs induces apoptosis
As part of the International Human Epigenome Consortium (IHEC), Mandoli et al. investigate the AML1-ETO-associated epigenome, transcriptome, and proteome in t(8;21) patient cells and cell lines. Together their results suggest that a balanced interplay between the chromatin environment and expression of RUNX1 and ERG prevent AML1-ETO oncogene overdose and thereby inhibit apoptosis. Explore the Cell Press IHEC webportal at www.cell.com/consortium/IHEC.
The Covid-19 a pandemic infectious disease and affected life across the world resulting in over 188.65 million confirmed cases across 223 countries, territories and areas with 4.06 million deaths. It ...is caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and spike (S) protein of SARS-CoV-2, which plays a key role in the receptor recognition and cell membrane fusion process, is composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (RBD) that recognizes and binds to the host receptor angiotensin-converting enzyme 2 (ACE2), while the S2 subunit mediates viral cell membrane fusion. Hence, it is a key target for developing neutralizing antibodies. Here, we have performed phylogenetic analysis and structural modeling of the SARS-CoV-2 spike glycoprotein, which is found highly conserved. The overall percent protein sequence identity from the SARS-CoV-2 spike protein sequences from the NCBI database was 99.68%. The functional domains of the S protein reveal that the S1 subunit was highly conserved (99.70%) than the S2 subunit (99.66%). Further, the 319–541 residues (RBD) of amino acids within the S1 domain were 100% similar among the spike protein. The 3D modeling of SARS-CoV-2 spike glycoprotein indicated that S protein has four domains with five protein units and the S1 subunit from 1 to 289 amino acid of domain 1 is highly conserved without any change in the ligand interaction site. This analysis clearly suggests that the S1 subunit (RBD 319–541) can be used as a target region for stable and safe vaccine development.
In this study of fecal samples from 1000 children below 6 years of age, 680 (68.0%) detected to have intestinal helminthic infection. The incidence of intestinal helminthiasis in urban group of ...children was 56.8% (284 out of 500 tested) while in rural group of children was 79.2% (396 out of 500 tested). Both in rural and urban population Ascaris lumbricoides was the single predominant species, whereas a combination of A. lumbricoides and Trichuris trichiura was common multiple infection. All cultures of fecal samples positive for hook worm ova revealed the prevalent species as Necator americanus in this area.
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