Super-Kamiokande (SK), a 50 kton water Cherenkov detector in Japan, is observing neutrinos and searching for proton decay and dark matter. The installation of new front-end electronics in 2008 marks ...the beginning of the 4th phase of SK (SK-IV). With the improvement of the water circulation system, calibration methods, reduction cuts, this phase achieved the lowest energy threshold thus far: 3.5 MeV kinetic energy. SK studies the effects of both the solar and terrestrial matter density on neutrino oscillations: a distortion of the solar neutrino energy spectrum would be caused by the edge of the Mikheyev-Smirnov-Wolfenstein resonance in the solar core, and terrestrial matter effects would induce a day/night solar neutrino flux asymmetry. SK observed solar neutrino interactions for more than 20 years. This long operation covers about ∼2 solar activity cycles. An analysis about a possible correlation between solar neutrino flux and 11 year activity cycle will be presented.
Amelogenin plays a crucial role in tooth enamel formation, and mutations on X-chromosomal amelogenin cause X-linked amelogenesis imperfecta (AI). Amelogenin pre–messenger RNA (mRNA) is highly ...alternatively spliced, and during alternative splicing, exon4 is mostly skipped, leading to the formation of a microRNA (miR-exon4) that has been suggested to function in enamel and bone formation. While delivering the functional variation of amelogenin proteins, alternative splicing of exon4 is the decisive first step to producing miR-exon4. However, the factors that regulate the splicing of exon4 are not well understood. This study aimed to investigate the association between known mutations in exon4 and exon5 of X chromosome amelogenin that causes X-linked AI, the splicing of exon4, and miR-exon4 formation. Our results showed mutations in exon4 and exon5 of the amelogenin gene, including c.120T>C, c.152C>T, c.155C>G, and c.155delC, significantly affected the splicing of exon4 and subsequent miR-exon4 production. Using an amelogenin minigene transfected in HEK-293 cells, we observed increased inclusion of exon4 in amelogenin mRNA and reduced miR-exon4 production with these mutations. In silico analysis predicted that Ser/Arg-rich RNA splicing factor (SRSF) 2 and SRSF5 were the regulatory factors for exon4 and exon5 splicing, respectively. Electrophoretic mobility shift assay confirmed that SRSF2 binds to exon4 and SRSF5 binds to exon5, and mutations in each exon can alter SRSF binding. Transfection of the amelogenin minigene to LS8 ameloblastic cells suppressed expression of the known miR-exon4 direct targets, Nfia and Prkch, related to multiple pathways. Given the mutations on the minigene, the expression of Prkch has been significantly upregulated with c.155C>G and c.155delC mutations. Together, we confirmed that exon4 splicing is critical for miR-exon4 production, and mutations causing X-linked AI in exon4 and exon5 significantly affect exon4 splicing and following miR-exon4 production. The change in miR-exon4 would be an additional etiology of enamel defects seen in some X-linked AI.
As described in this paper, we proposed the adoption of polyacrylic acid (PAA) with H2O, which becomes PAA + H2O mixture gel, as an ablation medium for arc quenching in a low-voltage DC circuit ...breaker. It is well known that H2O is a highly arc-quenching medium, but it has been difficult to use H2O in liquid phase directly as an arc-quenching medium. The arc performance of PAA + H2O mixture gel was studied using numerical and experimental approaches. Numerical thermofluid simulation was conducted to obtain the arc temperature and gas flow distribution at different H2O mixing ratios in PAA + H2O gel. To consider ablation of PAA + H2O gel, a method of alternately arranging PAA and H2O in the ablation wall was also proposed. Calculation results demonstrate that increasing the H2O mixing ratio in PAA + H2O mixture gel makes the arc narrower in the temperature and electrical conductivity distributions. The experimentally obtained results also indicate that arcs confined in PAA + H2O mixture gel with higher H2O mixture become narrower than those in PAA or other materials. Moreover, arcs confined by PAA + H2O mixture gel with higher H2O mixing ratio enhance the arc resistance rapidly. The results suggest that PAA + H2O mixture gel can have higher arc-quenching performance than pure polymers.
A numerical model was developed on the evaporation process of intermittently fed feedstock into pulse-modulated induction thermal plasma (PMITP). This experimental method combining the PMITP with ...intermittent feedstock feeding has been developed for the synthesis of large amounts of nanoparticles. To treat this method, a full transient numerical model for the PMITP was combined with transient equations of particle behaviour with different time steps. Transient two-way interactions from particle evaporation to the PMITP were also taken into account at each time step as source terms in mass, momentum and energy conservations. The temperature, gas flow field and mass fraction of feedstock vapor were obtained in the transient state in the PMITP with the motion of feedstock particles. The calculation results were compared with the experimental results for the Ar I line and feedstock atomic line. They were found to be in fair agreement even in the transient state.
Since the conventional coherent transceiver is costly to be deployed in short-reach networks due to its complicated receiver structure, it is desired to simplify the structure itself. In this paper, ...we propose a simple polarization-diversity coherent receiver structure by exploiting the concept of the Stokes analyzer. Compared to the conventional architecture, the number of the photodiodes (PDs) is reduced from eight to six without relying on complicated analog circuits. In addition, splitters and combiners for dual-polarization (DP) signals can be replaced with only one polarization beam splitter or combiner (PBS/C). For evaluation of the proof-of-concept (PoC), we developed a prototype of the receiver using free-space optical components. We demonstrate the transmission of 120-Gb/s DP quadrature phase-shift keying (QPSK) and DP 8-ary quadrature-amplitude modulation (8QAM) signals over a 100-km single-mode fiber (SMF). We believe that the demonstrated architecture could potentially be integrated monolithically on silicon-photonic or InP platforms to realize compact and low-cost coherent transceivers for short-reach applications.
Hypophosphatasia (HPP) occurs from loss-of-function mutation in the tissue-non-specific alkaline phosphatase (TNALP) gene, resulting in extracellular pyrophosphate accumulation that inhibits skeletal ...and dental mineralization. TNALP-null mice (Akp2
-/-
) phenocopy human infantile hypophosphatasia; they develop rickets at 1 week of age, and die before being weaned, having severe skeletal and dental hypomineralization and episodes of apnea and vitamin B6-responsive seizures. Delay and defects in dentin mineralization, together with a deficiency in acellular cementum, are characteristic. We report the prevention of these dental abnormalities in Akp2
-/-
mice receiving treatment from birth with daily injections of a mineral-targeting, human TNALP (sALP-FcD10). sALP-FcD10 prevented hypomineralization of alveolar bone, dentin, and cementum as assessed by micro-computed tomography and histology. Osteopontin – a marker of acellular cementum – was immuno-localized along root surfaces, confirming that acellular cementum, typically missing or reduced in Akp2
-/-
mice, formed normally. Our findings provide insight concerning how acellular cementum is formed on tooth surfaces to effect periodontal ligament attachment to retain teeth in their osseous alveolar sockets. Furthermore, they provide evidence that this enzyme-replacement therapy, applied early in post-natal life – where the majority of tooth root development occurs, including acellular cementum formation – could prevent the accelerated tooth loss seen in individuals with HPP.
Abstract In addition to cognitive impairment, behavioral and psychological symptoms of dementia (BPSD) are another important aspect of most dementia patients. This study was designed for a new simple ...assessment of BPSD. We first employed a clinical survey for the local community with sending an inquiry letter to all members (n = 129) of dementia caregiver society, and then attempted to create a new BPSD score for dementia with 10 BPSD items. This new simple BPSD score was compared to a standard-detailed BPSD score neuropsychiatric inventory (NPI) for a possible correlation (n = 792) and a time to complete (n = 136). Inter-rater reliability was examined comparing scores between main and second caregivers (n = 70) for AD. Based on the clinical survey for local caregivers, a new BPSD score for dementia (ABS, Abe's BPSD score) was newly created, in which each BPSD item was allotted by an already-weighted score (maximum 1–9) based on the frequency and severity, and was finalized with taking temporal occurrences into account. ABS was filled by the main caregiver with a full score of 44, was well correlated with NPI (r = 0.716, **p < 0.01) in 792 AD patients (age 78.6 ± 7.0 years, MMSE 19.0 ± 5.9), and took a shorter time as only 56.8 ± 38.8 s (**p < 0.01) than NPI score (132.7 ± 94.0 s) with 136 AD patients. A high inter-rater reliability was obtained (r = 0.964, **p < 0.01) with a little smaller score (0.877 time) of ABS in secondary than the main caregivers. ABS provides a new simple and quick test for BPSD assessment, with a good correlation to NPI but a shorter time, and with a high inter-rater reliability. Thus ABS is useful for evaluating BPSD for mild to moderate dementia patients.
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