We propose an approach to obtain the cell receptor for the tick-borne encephalitis (TBE) virus by affinity chromatography using polyclonal antiidiotyping antibodies (AlAbs) as antireceptor ...antibodies. The purified fraction of the cell receptor contains four polypeptides with molecular weights of 43, 67, 110, and 210 kDa. Polyclonal AlAbs interacted only with the 67 kDa protein in immunoblotting. The molecular size of the 67 kDa protein suggested that it is the nonintegrin laminin binding protein (LBP).
Using the polymerase chain reaction and appropriate primers, the amplified fragments, containing the gene of the human laminin binding protein, were cloned from the RH cells; a gene-engineering product of the recombinant laminin binding protein was developed. The highly purified recombinant LBP interacted with the native protein E of the TBE virus and also competed with monoclonal antibody for interaction with protein E. The affinity of the interaction of the recLBP with protein E of the TBE virus was estimated to be 0.5–5 x 10
7 M
‡1.
The complete genomic sequence (minus primer-generated ends) of the laboratory-adapted Crimean Congo hemorrhagic fever virus (CCHFV) strain ROS/HUVLV-100, isolated in 2003 from the blood of a deceased ...female from the Rostov region of southern European Russia, was determined by direct sequencing of overlapping reverse transcription/polymerase chain reaction amplified products. The size of the ROS/HUVLV-100 genome is 19.2 kilobases--individual genome segments are similar in size and sequence features to previously reported "Europe-1" group CCHFV strains. The low-passage ROS/HUVLV-100 strain is the first Russian Crimean Congo hemorrhagic fever virus isolate for which complete sequence information is available, and this work reports the first complete genomic CCHFV sequence determined from a single viral RNA preparation in the same laboratory.
