Primary hyperparathyroidism (PHPT) is a common endocrine disorder caused by a parathyroid tumor or hyperplasia, which is often accompanied with quality of life (QoL) impairment. A parathyroidectomy ...(PTX) is the preferred standard treatment for PHPT patients. In this single center study we aimed to evaluate the impact of PHPT on patient's QoL and identify QoL changes at early and long-term follow-up after surgery.
All the patients underwent routine PTX with the removal of the suspected hyperparathyroid gland(s). Patients filled out generic QoL questionnaire RAND SF-36, specific questionnaire PHPQoL and specific symptom assessment questionnaire PAS upon admission to the hospital before surgery, at 3 months, 12 months and 24 months after surgery.
A total of 92 patients with PHPT (median age was 56 years, 95.7% females) were included in the study. Before PTX patient's QoL by SF-36 scores was significantly lower as compared to healthy controls (p < 0.01). Almost 40% of patients had poor or very poor QoL. The most frequent symptoms by PAS before surgery were as follows: tiredness (97.8% of patients), weakness (94.6%), forgetfulness (94.6%), mood changes (90%), feeling "blue"/depression (88%), joint pains (83.3%), headaches (80.2%), constant irritability (77.2%), bone pains (75%), thirst (70.7%) and trouble getting out of a chair (67.4%). The half of the patients had moderate-to-severe (≥ 40 scores) tiredness, weakness, joint pains, forgetfulness, as well as mood changes. Post-operative QoL changes were analysed in the group of 72 patients. After surgery there was significant improvement in QoL by all scales of SF-36 questionnaire, excluding bodily pain, and the PHPQoL total score (GEE, p < 0.01) as compared with their values before surgery. Also severity of tiredness, mood changes, weakness and forgetfulness significantly decreased after surgery as compared to their baseline values (GEE, p < 0.05). Decreased mental component of QoL by PHPQoL (OR = 0.927, 95%CI = 0.874-0.984, p = 0.013) predicted improved QoL after surgery.
Patients with PHPT demonstrated significantly impaired QoL in physical, psychological and social functioning as well experienced a wide profile of common PHPT symptoms. Successful PTX was accompanied with remarkable QoL improvement and decrease in subjective symptoms for at least 24 months after surgery.
The method of accuracy improving and uncertain plant parameters sensitivity reducing based on multiobjective synthesis of two degree offreedom nonlinear robust control by discrete-continuous plant is ...developed. Synthesis of nonlinear robust regulators and nonlinear robust observers reduces to Hamilton-Jacobi-Isaacs equations solution. The robust control target vector is choiced by multicriterion nonlinear programming problem solution in which the objective function vectors is direct indexes performance vector that are presented to the system in various modes of its operation. The robust control target vector calculated by synthesized nonlinear robust control system modeling for various modes of system operation with different input signals and for various plant parameters values. The dynamic characteristics modeling end experimental researching results of a synthesized nonlinear electromechanical servo system for system operation various modes with different input signals and for plant parameters various values are given. References 8, figure 1.
Human ring chromosomes are often unstable during mitosis, and daughter cells can be partially or completely aneuploid. We studied the mitotic stability of four ring chromosomes, 8, 13, 18, and 22, in ...long-term cultures of skin fibroblasts and induced pluripotent stem cells (iPSCs) by GTG karyotyping and aCGH. Ring chromosome loss and secondary aberrations were observed in all fibroblast cultures except for r(18). We found monosomy, fragmentation, and translocation of indexed chromosomes. In iPSCs, aCGH revealed striking differences in mitotic stability both between iPSC lines with different rings and, in some cases, between cell lines with the same ring chromosome. We registered the spontaneous rescue of karyotype 46,XY,r(8) to 46,XY in all six iPSC lines through ring chromosome loss and intact homologue duplication with isoUPD(8)pat occurrence, as proven by SNP genotype distribution analysis. In iPSCs with other ring chromosomes, karyotype correction was not observed. Our results suggest that spontaneous correction of the karyotype with ring chromosomes in iPSCs is not universal and that pluripotency is compatible with a wide range of derivative karyotypes. We conclude that marked variability in the frequency of secondary rearrangements exists in both fibroblast and iPSC cultures, expanding the clinical significance of the constitutional ring chromosome.
The article is devoted to the influence of brown coal additives on the sintering of the lime-nepheline charge in the technology of alumina production from nepheline. The process of extraction of ...humic compounds from brown coal is considered, the effect of humic additives on the rheological properties of pulp depending on the size and moisture content of carbon-containing additives is studied.
Ring chromosome 18 is a rare chromosomal disorders that usually originate de novo and correlate with clinical manifestation: developmental delay as well as microcephaly, brain and ocular ...malformations, hypotonia and skeletal abnormalities. We generate iPSC clonal cell line ICGi024-A with pluripotency properties which were demonstrated in vitro by three germ layer differentiation capacity. ICGi024-A can be used for disease modeling and fundamental investigation of ring chromosome instability.
Aim.Development of method for design a two-level active shielding system for an industrial frequency magnetic field based on a quasi-static model of a magnetic field generated by power line wires and ...compensating windings of an active shielding system, including coarse open and precise closed control. Methodology. At the first levelroughcontrol of the magnetic field in open-loop form is carried out based on a quasi-static model of a magnetic field generated by power line wires and compensating windings of an active shielding system. This design calculated based on the finite element calculations system COMSOL Multiphysics. At the second level, a stabilizingaccurate control of the magnetic field is implemented in the form of a dynamic closed system containing, in addition plant, also power amplifiers and measuring devices of the system. This design calculated based on the calculations system MATLAB. Results. The results of theoretical and experimental studies of optimal two-level active shielding system of magnetic field in residential building from power transmission line with a «Barrel» type arrangement of wires by means of active canceling with single compensating winding are presented. Originality. For the first time, the method for design a two-level active shielding system for an power frequency magnetic field based on a quasi-static model of a magnetic field generated by power line wires and compensating windings of an active shielding system, including coarse open and precise closed control is developed. Practical value. It is shown the possibility to reduce the level of magnetic field induction in residential building from power transmission line with a «Barrel» type arrangement of wires by means of active canceling with single compensating winding with initial induction of 3.5 µT to a safe level for the population adopted in Europe with an induction of 0.5 µT.
Wilson’s disease is a rare autosomal recessive disorder of copper metabolism. The copper accumulation in the viscera appears due to the functional impairment of copper-transporting ATPase, which is ...encoded by the ATP7B gene. In this study, PBMCs of a patient with two ATP7B mutations were reprogrammed. The first mutation is a missense mutation p.H1069Q, which is the most frequent mutation in the human population. At the same time, the second one is a frameshift mutation p.Lys1013fs. The generated iPSC line had a normal karyotype, maintained the original genotype, expressed pluripotency markers, and demonstrated the ability to differentiate into derivatives of the three germ layers.
Aim. Development of the method of designing a combined electromagnetic shield, consisting of active and passive parts, to improve the effectiveness of reduction of industrial frequency magnetic field ...created by two-circuit overhead power lines in residential buildings.Methodology.The problem ofdesignofcombinedelectromagnetic shieldincluding robust system of active shielding and electromagnetic passive shield of initial magnetic fieldsolved based on of the multi-criteria two-player antagonistic game. The game payoff vector calculated based on thefinite element calculations systemCOMSOL Muliphysics. The gamesolutioncalculated based on the particles multiswarm optimization algorithms. During the designof combined electromagnetic shields spatial location coordinatesof shielding winding,the currents andphasesin the shielding windingof active shielding,geometric dimensionsandthickness of theelectromagneticpassiveshieldarecalculated.Results.The results of theoretical and experimentalstudies ofcombinedelectromagnetic passive and active shielding of magnetic field inresidentialbuilding from power transmission line with a«Barrel» typearrangement of wirespresented. Originality.For the first timethe method of designing a combined electromagnetic shield, consisting of active and passive parts, for more effective reduction of the magnetic field of industrial frequency created by two-circuit overhead power lines in residential buildingsis developed.Practical value. Based onresults of calculated and experimental study the shielding efficiency of the initial magnetic field determined that shielding factorswhith onlyelectromagnetic passive shield is more 2 units,whith onlyactive shield is more 4 units andwithcombined electromagnetic passive and active shield is more 10 units.Itis shown the possibility to reduce the level of magnetic field inductioninresidentialbuilding from power transmission line with a«Barrel» typearrangement of wires by means of acombinedelectromagnetic passive and active shielding with single compensating winding to 0.5μTlevel safe for the population. References 53, figures 15.
Purpose
To study the contribution of embryo chromosomal abnormalities in primary and secondary recurrent pregnancy loss (RPL) and to analyze the recurrence of chromosomal constitution in miscarriages ...from the same couple.
Methods
Retrospective study of abortion karyotypes in RPL families based on the mother’s primary or secondary RPL status (563 embryo specimens, 335 samples from primary, and 228 samples from secondary RPL). RPL was defined as two or more consecutive miscarriages. One hundred eight cases of recurrent embryo/fetal loss in 51 families were analyzed to assess the probability of having the same karyotype pattern (recurrent normal or recurrent abnormal) in both previous and subsequent pregnancy loss. The karyotypes of abortions were established using standard cytogenetic analysis, as well as interphase fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH).
Results
The frequency of aberrations was 43.9% in abortions from primary RPL versus 52.6% in secondary RPL (
p
= 0.041). Women 35 years of age or older were the main contributors to this difference. The odds ratio of a subsequent abortion having the same karyotype pattern (normal or abnormal) as the previous one was 6.98 (
p
= 0.0013).
Conclusion
The frequency of abnormalities is higher in abortions from the secondary RPL versus primary RPL group, and this difference is due to the relative deficiency of miscarriages with abnormal karyotypes in older women with primary RPL. The probability of having the same karyotype pattern (recurrent normal or recurrent abnormal) in the previous and subsequent abortion is increased significantly compared with chance.
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