Streaks have been found to be an important part of wall-turbulence dynamics. In this paper, we extend the analysis for unbounded shear flows, in particular a Mach 0.4 round jet, using measurements ...taken using dual-plane, time-resolved, stereoscopic particle image velocimetry (PIV) taken at pairs of jet cross-sections, allowing the evaluation of the cross-spectral density of streamwise velocity fluctuations resolved into azimuthal Fourier modes. From the streamwise velocity results, two analyses are performed: the evaluation of wavenumber spectra (assuming Taylor’s hypothesis for the streamwise coordinate) and a spectral proper orthogonal decomposition (SPOD) of the velocity field using PIV planes in several axial stations. The methods complement each other, leading to the conclusion that large-scale streaky structures are also present in turbulent jets where they experience large growth in the streamwise direction, energetic structures extending up to eight diameters from the nozzle exit. Leading SPOD modes highlight the large-scale, streaky shape of the structures, whose aspect ratio (streamwise over azimuthal length) is approximately 15. The data were further analysed using SPOD, resolvent and transient growth analyses, good agreement being observed between the models and the leading SPOD mode for the wavenumbers considered. The models also indicate that the lift-up mechanism is active in turbulent jets, with streamwise vortices leading to streaks. The results show that large-scale streaks are a relevant part of the jet dynamics.
Summary
Background
Worldwide, dengue is the most prevalent human arbovirus disease. Dengue infection may cause a range of clinical manifestations from self‐limiting febrile illness through to a ...life‐threatening syndrome accompanied by both bleeding and shock. Thrombocytopenia is frequently observed in mild and severe disease; however, the mechanisms involved in DENV‐induced platelet activation and thrombocytopenia are incompletely understood.
Patients and methods
Freshly isolated platelets from patients with dengue were evaluated for markers of activation, mitochondrial alteration and activation of cell death pathways. In parallel, we examined direct DENV‐induced activation and apoptosis of platelets obtained from healthy subjects.
Results
We found that platelets from DENV‐infected patients exhibited increased activation by comparison to control subjects. Moreover, platelets from DENV‐infected patients exhibited classic signs of the intrinsic pathway of apoptosis that include increased surface phosphatidylserine exposure, mitochondrial depolarization and activation of caspase‐9 and ‐3. Indeed, thrombocytopenia was shown to strongly associate with enhanced platelet activation and cell death in DENV‐infected patients. Platelet activation, mitochondrial dysfunction and caspase‐dependent phosphatidylserine exposure on platelets were also observed when platelets from healthy subjects were directly exposed to DENV in vitro. DENV‐induced platelet activation was shown to occur through mechanisms largely dependent on DC‐SIGN.
Conclusions
Together our results demonstrate that platelets from patients with dengue present signs of activation, mitochondrial dysfunction and activation of the apoptosis caspase cascade, which may contribute to the development of thrombocytopenia in patients with dengue. Our results also suggest the involvement of DC‐SIGN as a critical receptor in DENV‐dependent platelet activation.
Three amplification mechanisms present in turbulent jets, namely lift-up, Kelvin–Helmholtz and Orr, are characterized via global resolvent analysis and spectral proper orthogonal decomposition (SPOD) ...over a range of Mach numbers. The lift-up mechanism was recently identified in turbulent jets via local analysis by Nogueira
et al.
(
J. Fluid Mech.
, vol. 873, 2019, pp. 211–237) at low Strouhal number (
$St$
) and non-zero azimuthal wavenumbers (
$m$
). In these limits, a global SPOD analysis of data from high-fidelity simulations reveals streamwise vortices and streaks similar to those found in turbulent wall-bounded flows. These structures are in qualitative agreement with the global resolvent analysis, which shows that they are a response to upstream forcing of streamwise vorticity near the nozzle exit. Analysis of mode shapes, component-wise amplitudes and sensitivity analysis distinguishes the three mechanisms and the regions of frequency–wavenumber space where each dominates, finding lift-up to be dominant as
$St/m\rightarrow 0$
. Finally, SPOD and resolvent analyses of localized regions show that the lift-up mechanism is present throughout the jet, with a dominant azimuthal wavenumber inversely proportional to streamwise distance from the nozzle, with streaks of azimuthal wavenumber exceeding five near the nozzle, and wavenumbers one and two most energetic far downstream of the potential core.
We present an analysis of the linear stability characteristics of shock-containing jets. The flow is linearised around a spatially periodic mean, which acts as a surrogate for a mean flow with a ...shock-cell structure, leading to a set of partial differential equations with periodic coefficients in space. Disturbances are written using the Floquet ansatz and Fourier modes in the streamwise direction, leading to an eigenvalue problem for the Floquet exponent. The characteristics of the solution are directly compared with the locally parallel case, and some of the features are similar. The inclusion of periodicity induces minor changes in the growth rate and phase velocity of the relevant modes for small shock amplitudes. On the other hand, the eigenfunctions are now subject to modulation related to the periodicity of the flow. Analysis of the spatio-temporal growth rates led to the identification of a saddle point between the Kelvin–Helmholtz mode and the guided jet mode, characterising an absolute instability mechanism. Frequencies and mode shapes related to the saddle points for two conditions (associated with axisymmetric and helical modes) are compared with screech frequencies and the most energetic coherent structures of screeching jets, resulting in a good agreement for both. The analysis shows that a periodic shock-cell structure has an impulse response that grows upstream, leading to oscillator behaviour. The results suggest that screech can occur in the absence of a nozzle, and that the upstream reflection condition is not essential for screech frequency selection. Connections to previous models are also discussed.
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► Zeolites as nanocontainers for corrosion inhibitors. ► Ce (III) as corrosion inhibitor. ► pH as the trigger mechanism of cerium release from zeolites. ► Zeolites improved the ...barrier properties of the hybrid sol–gel films. ► Ce (III) releases from Ce–zeolite and precipitates on intermetallic inclusions.
This work presents a new approach to develop active protection coating systems. Zeolite microparticles were used as reservoirs for Ce (III). The zeolites were introduced into silica–zirconia sol–gel films, improving the barrier properties of the coating and conferring active corrosion protection to the AA2024 substrate. The corrosion process changes the pH of solution locally causing release of inhibitor that precipitates in the cathodic areas. Intermetallic inclusions seem to be sites prone to the formation of these cerium rich precipitates. The protection properties of the modified coatings were assessed using EIS.
In resolvent analyses of turbulent channel flows it has been common practice to neglect or model the nonlinear forcing term that forms the input of the resolvent. However, the spatiotemporal ...structure of this term is mostly unknown. Here, this nonlinear forcing term is quantified. The Fourier transform of its two-point space–time correlation, its cross-spectral density (CSD), is computed. The CSD is evaluated for two channel flows at friction Reynolds numbers $Re_{\tau } =179$ and $Re_{\tau } =543$ via direct numerical simulations (DNS). The CSDs are computed for energetic structures typical of buffer-layer and large-scale motions, for different temporal frequencies. It is found that the forcing is structured and that its solenoidal part, which is the only one affecting the velocity field, is the combination of an oblique streamwise vortical forcing and a streamwise component that counteract each other, as in a destructive interference. It is shown that a rank-2 approximation of the forcing, with only the most energetic spectral proper orthogonal decomposition (SPOD) modes, leads to the bulk of the response. Moreover, it is found that the nonlinear forcing term has a non-negligible projection onto the linear sub-optimal forcings of resolvent analysis, which demonstrates that the linear optimal forcing is not representative of the nonlinear forcing. Finally, it is clarified that the Cess eddy-viscosity-modelled forcing improves the accuracy of resolvent analysis prediction because the modelled forcing projects onto the linear sub-optimal forcings similarly to DNS data.
A model problem for analysing the interaction between coherent structures in shear flows with the presence of a convective instability is proposed in this work. Starting from Couette flow, a ...permanent forcing in the shape of a hyperbolic tangent is introduced in the laminar equations, leading to a wall-bounded flow with an inflection point, which triggers a hydrodynamic instability. Temporal linear stability analysis applied to this new flow model shows that this flow is unstable at low Reynolds numbers, giving rise to Kelvin–Helmholtz-like vortices. Due to the presence of shear, streaks and rolls (streamwise vortices), predicted by resolvent analysis, are also present in the flow, and these structures will interact with vortices via oblique waves. Results of locally parallel analysis inspired the design of a computational box for a direct numerical simulation of such flow and the numerical results exhibit a limit cycle involving streaks, vortices, rolls, oblique waves and the mean flow, so that the flow becomes periodically unstable for the present case. The flow dynamics is shown to reproduce some of the features of jets and mixing layers, such as jitter and translational instability, showing that the present model can potentially clarify some of the phenomena involved in the turbulent dynamics of such flows.
Abstract
An analysis of the statistics of the nonlinear terms in resolvent analysis is performed in this work for turbulent Couette flow at Reynolds number 400. Data from a direct numerical ...simulation of a minimal flow unit is used to compute the covariance matrix of the velocity. From the same data, we computed the nonlinear terms of the Navier–Stokes equations (treated as forcing), which allowed us to compute the covariance matrix of the forcing. The quantitative relation between the two covariances via the resolvent operator is confirmed here for the first time, accounting for relevant signal processing issues related to the windowing procedure for frequency-domain quantities. Such exact correspondence allowed the eduction of the most relevant force components for the dominant structures in this flow, which participate in the self-sustaining cycle of turbulence: (i) streamwise vortices and streaks, and (ii) spanwise-coherent fluctuations of spanwise velocity. The results show a dominance by a subset of the nonlinear terms for the prediction of the full statistics of streamwise vortices and streaks; a single term is seen to be dominant for spanwise motions. A relevant feature observed in these cases is that the forcing covariance is dominated by its first eigenfunction, showing that nonlinear terms also have a coherent structure at low frequencies in this flow. Different forcing components are also coherent between them, which leads to constructive and destructive interferences that greatly modify the flow response. These are key features of forcing ‘colour’ for the present flow.
The problem of finding optimal forcing and response for unbounded base flows, exemplified by the Blasius boundary layer, is assessed by means of a locally parallel resolvent analysis. A new analysis ...of previous results in the literature, which stated that a maximum resolvent gain occurs for spanwise wavenumber
k
z
≈
0.2
, revealed that this result was not domain converged, and larger domains lead to peak amplification for
k
z
→
0
; this result is seen to depend strongly on domain size. It is seen that forcing and response modes for low frequency and wavenumber tend to be extended throughout the computational domain, with substantial support in the free stream. Free-stream modes and their gains are found analytically by considering the resolvent operator for uniform flow, and it is seen that low frequencies and wavenumbers lead to a dominance of such free-stream modes in the resolvent analysis of boundary layers. The lack of domain convergence is explained by the analysis, as gains scale with the square of the domain height. We then propose a new approach to evaluate the resolvent gains for this kind of unbounded flows, by means of a weighting function for the chosen norm that neglects response modes above a cut-off height
y
p
, typically placed outside the boundary layer thickness; this ensures that relevant responses will only be sought in a region of interest, which here corresponds to the boundary layer. The method proved to solve the problem raised by the presence of free-stream modes, resulting in domain-converged forcing and response modes with the shape of streamwise vortices and streaks, respectively. The results were also shown to be independent of the choice of the filter parameters, leading to converged gains for the whole spectrum.
ABSTRACT This study aimed to evaluate methane emission, milk yield and behavior of ewes kept exclusively on irrigated pasture of Tifton 85 grass (Cynodon spp.) or supplemented with ground corn or ...whole cottonseed (WCS) based concentrates. Twelve Lacaune x Santa Ines ewes (43.07±0.83 kg of body weight, 77±24 days after parturition, on average) were distributed in replicated 3x3 Latin square. Treatments consisted of three diets: pasture (no concentrate supplementation); corn (pasture + corn-based supplement); whole cottonseed (pasture + whole cottonseed-based supplement), offering 0.5 kg/ewe/day. The WCS group showed the highest concentrate dry matter intake (DMI) (p=0.049) and crude protein (CP) intake (p=0.001) compared to the others. There was no difference on total DMI (p=0.115) for the tested diets. Animals exclusively kept on pasture had the greatest forage DMI (p=0.004), lowest CP digestibility (p=0.015), showed longer grazing time (p=0.01) and shorter idle time (p=0.01) compared to the supplemented groups. Milk yield (0.36 kg/ewe/day) (p=0.15) and methane emission (33.12 g/ewe/day) (p=0.95) were similar for all three evaluated groups. Supplementation with concentrate based on corn or whole cottonseed does not improve productive performance nor decrease methane emission. However, lactating ewes kept exclusively in pasture show longer grazing time, without changes in milk yield and methane emission.
RESUMO Objetivou-se avaliar a emissão de metano, a produção de leite e o comportamento de ovelhas mantidas exclusivamente em pastagem irrigada de Tifton 85 (Cynodon spp.) ou suplementadas com concentrados à base de milho grão ou caroço de algodão. Doze ovelhas Lacaune x Santa Inês (43,07±0,83 kg de peso corporal e 77±24 dias após a parição, em média) foram distribuídas em quadrados latinos 3x3 replicados. Os tratamentos consistiram em três dietas: pastagem (sem suplementação concentrada); milho (pastagem + suplemento à base de milho); caroço de algodão integral (pastagem + suplemento à base de caroço de algodão), oferecendo 0,5 kg/ovelha/dia. O grupo caroço de algodão apresentou maior consumo de matéria seca (CMS) (P=0,049) e consumo de proteína bruta (PB) (P=0,001) em relação aos demais. Não houve diferença no CMS total (P=0,115) para as dietas testadas. Os animais exclusivamente em pastagem apresentaram maior consumo de forragem (P=0,004), menor digestibilidade da PB (P=0,015), maior tempo em pastejo (P=0,01) e menor tempo em ócio (P=0,01), em relação aos grupos suplementados. A produção de leite (0,36 kg/ovelha/dia) (P=0,15) e a emissão de metano (33,12 g/ovelha/dia) (P=0,95) foram semelhantes nos três grupos avaliados. A suplementação com concentrado à base de milho grão ou caroço de algodão não melhorou o desempenho produtivo e não reduziu a emissão de metano. Entretanto, as ovelhas lactantes mantidas exclusivamente em pastagem apresentaram maior tempo em pastejo, sem alterações na produção de leite e na emissão de metano.
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