We report the detection of steady radio emission from the known X-ray source X9 in the globular cluster 47 Tuc. With a double-peaked C iv emission line in its ultraviolet spectrum providing a clear ...signature of accretion, this source had been previously classified as a cataclysmic variable. In deep ATCA (Australia Telescope Compact Array) imaging from 2010 and 2013, we identified a steady radio source at both 5.5 and 9.0 GHz, with a radio spectral index (defined as S
ν ∝ να) of α = −0.4 ± 0.4. Our measured flux density of 42 ± 4 μJy beam−1 at 5.5 GHz implies a radio luminosity (νL
ν) of 5.8 × 1027 erg s−1, significantly higher than any previous radio detection of an accreting white dwarf. Transitional millisecond pulsars, which have the highest radio-to-X-ray flux ratios among accreting neutron stars (still a factor of a few below accreting black holes at the same L
X), show distinctly different patterns of X-ray and radio variability than X9. When combined with archival X-ray measurements, our radio detection places 47 Tuc X9 very close to the radio/X-ray correlation for accreting black holes, and we explore the possibility that this source is instead a quiescent stellar-mass black hole X-ray binary. The nature of the donor star is uncertain; although the luminosity of the optical counterpart is consistent with a low-mass main-sequence donor star, the mass transfer rate required to produce the high quiescent X-ray luminosity of 1033 erg s−1 suggests the system may instead be ultracompact, with an orbital period of order 25 min. This is the fourth quiescent black hole candidate discovered to date in a Galactic globular cluster, and the only one with a confirmed accretion signature from its optical/ultraviolet spectrum.
Human respiratory syncytial virus (RSV) is the leading cause of respiratory tract infections in children globally, with nearly all children experiencing at least one infection by the age of two. ...Partial sequencing of the attachment glycoprotein gene is conducted routinely for genotyping, but relatively few whole genome sequences are available for RSV. The goal of our study was to sequence the genomes of RSV strains collected from multiple countries to further understand the global diversity of RSV at a whole-genome level.
We collected RSV samples and isolates from Mexico, Argentina, Belgium, Italy, Germany, Australia, South Africa, and the USA from the years 1998-2010. Both Sanger and next-generation sequencing with the Illumina and 454 platforms were used to sequence the whole genomes of RSV A and B. Phylogenetic analyses were performed using the Bayesian and maximum likelihood methods of phylogenetic inference.
We sequenced the genomes of 34 RSVA and 23 RSVB viruses. Phylogenetic analysis showed that the RSVA genome evolves at an estimated rate of 6.72 × 10(-4) substitutions/site/year (95% HPD 5.61 × 10(-4) to 7.6 × 10(-4)) and for RSVB the evolutionary rate was 7.69 × 10(-4) substitutions/site/year (95% HPD 6.81 × 10(-4) to 8.62 × 10(-4)). We found multiple clades co-circulating globally for both RSV A and B. The predominant clades were GA2 and GA5 for RSVA and BA for RSVB.
Our analyses showed that RSV circulates on a global scale with the same predominant clades of viruses being found in countries around the world. However, the distribution of clades can change rapidly as new strains emerge. We did not observe a strong spatial structure in our trees, with the same three main clades of RSV co-circulating globally, suggesting that the evolution of RSV is not strongly regionalized.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Context. Intermediate-mass black holes (IMBHs) are of interest in a wide range of astrophysical fields. In particular, the possibility of finding them at the centers of globular clusters has recently ...drawn attention. IMBHs became detectable since the quality of observational data sets, particularly those obtained with HST and with high resolution ground based spectrographs, advanced to the point where it is possible to measure velocity dispersions at a spatial resolution comparable to the size of the gravitational sphere of influence for plausible IMBH masses. Aims. We present results from ground based VLT/FLAMES spectroscopy in combination with HST data for the globular cluster NGC 6388. The aim of this work is to probe whether this massive cluster hosts an intermediate-mass black hole at its center and to compare the results with the expected value predicted by the M• − σ scaling relation. Methods. The spectroscopic data, containing integral field unit measurements, provide kinematic signatures in the center of the cluster while the photometric data give information of the stellar density. Together, these data sets are compared to dynamical models and present evidence of an additional compact dark mass at the center: a black hole. Results. Using analytical Jeans models in combination with various Monte Carlo simulations to estimate the errors, we derive (with 68% confidence limits) a best fit black-hole mass of (17 ± 9) × 103 M⊙ and a global mass-to-light ratio of M/LV = (1.6 ± 0.3) M⊙/L⊙.
Respiratory syncytial virus (RSV) is a major cause of respiratory infections and is classified in two main groups, RSV-A and RSV-B, with multiple genotypes within each of them. For RSV-B, more than ...30 genotypes have been described, without consensus on their definition. The lack of genotype assignation criteria has a direct impact on viral evolution understanding, development of viral detection methods as well as vaccines design. Here we analyzed the totality of complete RSV-B G gene ectodomain sequences published in GenBank until September 2018 (n = 2190) including 478 complete genome sequences using maximum likelihood and Bayesian phylogenetic analyses, as well as intergenotypic and intragenotypic distance matrices, in order to generate a systematic genotype assignation. Individual RSV-B genes were also assessed using maximum likelihood phylogenetic analyses and multiple sequence alignments were used to identify molecular markers associated to specific genotypes. Analyses of the complete G gene ectodomain region, sequences clustering patterns, and the presence of molecular markers of each individual gene indicate that the 37 previously described genotypes can be classified into fifteen distinct genotypes: BA, BA-C, BA-CC, CB1-THB, GB1-GB4, GB6, JAB1-NZB2, SAB1, SAB2, SAB4, URU2 and a novel early circulating genotype characterized in the present study and designated GB0.
Antecedentes: En México, la enfermedad de Still del adulto (ESA) es una causa de fiebre de origen desconocido (FOD). El objetivo de este informe fue describir una serie de casos de ESA de una cohorte ...de FOD para conocer las características clínicas y bioquímicas, así como describir el índice neutrófilo/linfocito (INL), marcador clínico de inflamación en enfermedades autoinmunes.Caso clínico: Estudio observacional de 24 casos con ESA; 72 % fue del sexo femenino, la edad fue de 43 años (37.7-59.7) y las manifestaciones más frecuentes fueron rash clásico (84 %) y artralgias (100 %). Todos tuvieron factor reumatoide, anticuerpos antinucleares negativos e hiperferritinemia; 83 % tuvo INL > 3.08. El tratamiento más empleado fue la combinación de metotrexato y corticosteroides; siete pacientes ameritaron terapia biológica, uno presentó reacción de hipersensibilidad.Conclusión: Ante fiebre de origen desconocido, debe sospecharse ESA si, además, existe rash, artralgias, hiperferritinemia e INL > 3.08.
Context. The formation of supermassive black holes at high redshift still remains a puzzle to astronomers. No accretion mechanism can explain the fast growth from a stellar mass black hole to several ...billion solar masses in less than one Gyr. The growth of supermassive black holes becomes reasonable only when starting from a massive seed black hole with mass on the order of 102−105 M⊙. Intermediate-mass black holes are therefore an important field of research. Especially the possibility of finding them in the centers of globular clusters has recently drawn attention. Searching for kinematic signatures of a dark mass in the centers of globular clusters provides a unique test for the existence of intermediate-mass black holes and will shed light on the process of black-hole formation and cluster evolution. Aims. We are investigating six galactic globular clusters for the presence of an intermediate-mass black hole at their centers. Based on their kinematic and photometric properties, we selected the globular clusters NGC 1851, NGC 1904 (M 79), NGC 5694, NGC 5824, NGC 6093 (M 80), and NGC 6266 (M 62). Methods. We used integral field spectroscopy to obtain the central velocity-dispersion profile of each cluster. In addition we completed these profiles with outer kinematic points from previous measurements for the clusters NGC 1851, NGC 1094, NGC 5824, and NGC 6093. We also computed the cluster photometric center and the surface brightness profile using HST data. After combining these datasets we compared them to analytic Jeans models. We used varying M/LV profiles for clusters with enough data points in order to reproduce their kinematic profiles in an optimal way. Finally, we varried the mass of the central black hole and tested whether the cluster is better fitted with or without an intermediate-mass black hole. Results. We present the statistical significance, including upper limits, of the black-hole mass for each cluster. NGC 1904 and NGC 6266 provide the highest significance for a black hole. Jeans models in combination with a M/LV profile obtained from N-body simulations (in the case of NGC 6266) predict a central black hole of M• = (3 ± 1) × 103 M⊙ for NGC 1904 and M• = (2 ± 1) × 103 M⊙ for NGC 6266. Furthermore, we discuss the possible influence of dark remnants and mass segregation at the center of the cluster on the detection of an IMBH.
Human Parainfluenza viruses (HPIV) type 1 and 3 are important causes of respiratory tract infections in young children globally. HPIV infections do not confer complete protective immunity so ...reinfections occur throughout life. Since no effective vaccine is available for the two virus subtypes, comprehensive understanding of HPIV-1 and HPIV-3 genetic and epidemic features is important for diagnosis, prevention, and treatment of HPIV-1 and HPIV-3 infections. Relatively few whole genome sequences are available for both HPIV-1 and HPIV-3 viruses, so our study sought to provide whole genome sequences from multiple countries to further the understanding of the global diversity of HPIV at a whole-genome level. We collected HPIV-1 and HPIV-3 samples and isolates from Argentina, Australia, France, Mexico, South Africa, Switzerland, and USA from the years 2003-2011 and sequenced the genomes of 40 HPIV-1 and 75 HPIV-3 viruses with Sanger and next-generation sequencing with the Ion Torrent, Illumina, and 454 platforms. Phylogenetic analysis showed that the HPIV-1 genome is evolving at an estimated rate of 4.97 × 10-4 mutations/site/year (95% highest posterior density 4.55 × 10-4 to 5.38 × 10-4) and the HPIV-3 genome is evolving at a similar rate (3.59 × 10-4 mutations/site/year, 95% highest posterior density 3.26 × 10-4 to 3.94 × 10-4). There were multiple genetically distinct lineages of both HPIV-1 and 3 circulating on a global scale. Further surveillance and whole-genome sequencing are greatly needed to better understand the spatial dynamics of these important respiratory viruses in humans.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Staphylococcus aureus is a leading cause of broad-spectrum infections both in the community and within healthcare settings. Methicillin-resistant Staphylococcus aureus (MRSA) has become a global ...public health issue. The aim of this study was to examine the clinical and molecular characteristics of Staphylococcus aureus isolates and to define the population structure and distribution of major MRSA clones isolated in a tertiary-care hospital in Mexico.
From April 2017 to April 2018, 191 Staphylococcus aureus isolates were collected. The frequency of MRSA was 26.7%; these strains exhibited resistance to clindamycin (84.3%), erythromycin (86.2%), levofloxacin (80.3%), and ciprofloxacin (86.3%). The majority of MRSA strains harbored the SCCmec type II (76.4%) and t895 (56.8%) and t9364 (11.7%) were the most common spa types in both hospital-associated MRSA and community-associated MRSA isolates. ST5-MRSA-II-t895 (New York /Japan clone) and ST1011-MRSA-II-t9364 (New York /Japan-Mexican Variant clone) were the most frequently identified clones. Furthermore, different lineages of Clonal Complexes 5 (85.4%) and 8 (8.3%) were predominantly identified in this study.
Our study provides valuable information about the epidemiology of MRSA in a city of the central region of Mexico, and this is the first report on the association between t895 and t9364 spa types and ST5 and ST1011 lineages, respectively. These findings support the importance of permanent surveillance of MRSA aimed to detect the evolutionary changes of the endemic clones and the emergence of new strains.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
During the COVID-19 pandemic, nonpharmaceutical interventions (NPIs) were implemented in order to control the transmission of SARS-CoV-2, potentially affecting the prevalence of respiratory syncytial ...virus (RSV). This review evaluated the impact of NPIs on RSV-related hospitalizations in children during the lockdown (2020-2021) compared to the pre-pandemic (2015-2020) and post-lockdown (2021-2022) periods. In this systematic review and meta-analysis, we searched through PubMed, Scopus, and Web of Science for studies published in English between 1 January 2015 and 31 December 2022. Additionally, we conducted hand searches of other records published between 1 January 2023 and 22 January 2024. Our target population was hospitalized children aged 0-18 years with RSV-related lower respiratory tract infections confirmed through immunofluorescence, antigen testing, or molecular assays. We focused on peer-reviewed observational studies, analyzing the primary outcome of pooled RSV prevalence. A generalized linear mixed model with a random-effects model was utilized to pool each RSV prevalence. Heterogeneity was assessed using Cochran's Q and
statistics, while publication bias was evaluated through funnel plots and Egger's tests. We identified and analyzed 5815 publications and included 112 studies with 308,985 participants. Notably, RSV prevalence was significantly lower during the lockdown period (5.03% 95% CI: 2.67; 9.28) than during the pre-pandemic period (25.60% 95% CI: 22.57; 28.88,
< 0.0001). However, RSV prevalence increased notably in the post-lockdown period after the relaxation of COVID-19 prevention measures (42.02% 95% CI: 31.49; 53.33 vs. 5.03% 95% CI: 2.67; 9.28,
< 0.0001). Most pooled effect estimates exhibited significant heterogeneity (
: 91.2% to 99.3%). Our findings emphasize the effectiveness of NPIs in reducing RSV transmission. NPIs should be considered significant public health measures to address RSV outbreaks.
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