Essential mixed cryoglobulinemia is frequently associated with hepatitis C virus (HCV) infection, with the formation of HCV antigen/antibody complexes. The hypervariable region (HVR) of the HCV ...E2/NS1 region is thought to include epitopes for neutralizing antibodies, but it remains uncertain whether cryoglobulins (CGs) contain such antibody-bound HCV. Thus, we studied HVR clones isolated from cryoprecipitate and supernatant in the sera of four chronic hepatitis C patients with cryoglobulinemia, and expressed as fusion proteins with glutathione S-transferase (GST). Patients' sera were tested for antibody binding to the proteins. The rate of anti-HVR antibody-positive clones was significantly higher in cryoprecipitate (89% +/- 13%, P < .05) than in supernatant (41% +/- 25%). Both HCV RNA and anti-HVR antibody were more concentrated in cryoprecipitates compared with those of serum and supernatant in two patients tested. Anti-HVR antibody-positive clones in cryoprecipitate showed common amino acid (aa) sequences in each of the four patients. Similarly, all the antibody-positive clones in supernatant showed the same aa sequences for three of the four patients. When aa sequences were compared with those of reported isolates with genotype 1b, the mean percentage of aa difference was greater in the clones from supernatant and in anti-HVR antibody-negative clones than in the clones from cryoprecipitate and in the antibody-positive clones, respectively. These findings indicate that serum CG contains anti-HVR antibody-bound HCV in patients with chronic hepatitis C. Anti-HVR antibody-free individual clones, which were more frequently noted in supernatant, showed closely related sequences, but which were of a heterogeneous quasispecies nature.
The genotoxicity of a pyrene oxide, 1-nitropyrene (NP) oxides and other related compounds was examined in the hepatocyte primary culture (HPC)/DNA repair test. Pyrene 4,5-oxide and both ...1-NP-4,5-oxide and 1-NP-9,10-oxide elicited clearly positive responses of DNA repair. In this assay, 1-NP itself was weakly positive. However, other related chemicals such as pyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene, and 1-nitro-8-hydroxypyrene did not generate positive responses.
We report here a 9-year-old boy presenting with absence and complex partial seizures. Absence seizures occurred several times a day, with sudden arrest of speech and gesture, alteration of ...consciousness, myoclonus of unilateral or bilateral angles of the mouth, occasional simple automatism and brisk recovery of consciousness. Complex partial seizures occurred once to three times a month with loss of consciousness, salivation, deviation of the head and eyes toward the left, elevation of upper limbs and tonic convulsion of the left upper and lower limbs. Interictal EEG showed right frontal pole-dominant high-voltage slow waves or spike-and-waves. Ictal simultaneous video-EEG recordings of absence seizures revealed a frontal dominant 3-3.5 Hz spike-wave burst lasting several seconds. A partial seizure never preceded the absence seizure. Transverse topographical analysis revealed that the first spike component of the spike-wave burst of absence seizure always showed phase reversal on the right anterior temporal electrode. The following ones, however, showed phase reversal on the left anterior temporal electrode. Ictal EEG of the complex partial seizure could not be detected because it rarely occurred. There was no abnormal finding on brain MRI. Interictal single photon emission tomography (SPECT) indicated hypoperfusion of the dorsal and medial cortex of the right middle frontal lobe. Interictal positron emission tomography (PET) also indicated hypometabolic areas in the dorsal and medial cortex of the right frontal lobe, together with those in the right temporal and parietal cortex. EEG evolution and neuroimaging studies suggested that the epileptic focus of the absence seizure might have originated at the dorsal cortex of the right middle frontal lobe and immediately spread to the medial cortex. Both the seizures were well controlled by the combination of phenytoin and high dose sodium valproate.
A study of the interstellar medium (ISM) and cosmic rays (CRs) using Fermi Large Area Telescope (LAT) data, in a region encompassing the nearby molecular clouds MBM 53, 54, and 55 and a far-infrared ...loop-like structure in Pegasus, is reported. By comparing Planck dust thermal emission model with Fermi-LAT gamma-ray data, it was found that neither the dust radiance (R) nor the dust opacity at 353 GHz (tau353) were proportional to the total gas column density N(Htot) primarily because N(Htot)/R and N(Htot)/tau353 depend on the dust temperature (Td). The N(Htot) distribution was evaluated using gamma-ray data by assuming the regions of high Td} to be dominated by optically thin atomic hydrogen (HI) and by employing an empirical linear relation of N(Htot)/R to Td. It was determined that the mass of the gas not traced by the 21-cm or 2.6-mm surveys is ~25% of the mass of HI in the optically thin case and is larger than the mass of the molecular gas traced by carbon monoxide by a factor of up to 5. The measured gamma-ray emissivity spectrum is consistent with a model based on CR spectra measured at the Earth and the nuclear enhancement factor of <=1.5. It is, however, lower than local HI emissivities reported by previous Fermi-LAT studies employing different analysis methods and assumptions on ISM properties by 15%-20% in energies below a few GeV, even if we take account of the statistical and systematic uncertainties. The origin of the discrepancy is also discussed.
A polymorphism in the angiotensin I-converting enzyme (ACE) gene has been associated with cerebrovascular diseases as a new potent risk factor. The purpose of this study was to investigate an ...association of the gene polymorphism with intracranial saccural aneurysmal patients. The study population consisted of 83 aneurysmal patients (age range 47-85 years) (the AN group) and 704 matched control subjects (age range 30-87 years) (the Control group). For detection of the ACE gene polymorphism, the standard PCR method was performed by using genomic DNA isolated from peripheral blood leukocytes. The PCR products were a 490-bp in the presence of the insertion (I) and a 190-bp fragment in the absence ofthe insertion (0). The ACE gene polymorphism was classified into three genotypes: 1,1 genotype (a 490-bp band); b,o genotype (a 790-bp band); or 1,0 genotype (both a 490-bp and a 190-bp band). The number of subjects with lilt 1/0, and % genotypes was 38, 40, and 5 in, the AN group and 43, 45, and 76 in the Control group, respectively. The frequency of the % genotype in the AN group was significantly lower (5/83 = 0.06) than that in the Control group (76/704 = 0.75) (X
2
= 4.06; P = 0.044). There was no significant difference between the genotypes of hypertensive patients and normotensive patients in the AN group. Thus, this present study suggests that genetic heterogeneity of the ACE gene may be correlated with the etiology of intracranial aneurysms. Neural Res 1998; 20: 607-611
PDF
