Climate change exacerbates abiotic stresses like salinization, negatively impacting crop yield, so development of strategies, like using salt-tolerant rootstocks, is crucial. The CALCINEURIN B-LIKE ...10 (SlCBL10) gene has been previously identified as a positive regulator of salt tolerance in the tomato shoot. Here, we report a different function of SlCBL10 in tomato shoot and root, as disruption of SlCBL10 only induced salt sensitivity when it was used in the scion but not in the rootstock. The use of SlCBL10 silencing rootstocks (Slcbl10 mutant and RNAi line) improved salt tolerance on the basis of fruit yield. These changes were associated with improved Na
and K
homoeostasis, as SlCBL10 silencing reduced the Na
content and increased the K
content under salinity, not only in the rootstock but also in the shoot. Improvement of Na
homoeostasis in Slcbl10 rootstock seems to be mainly due to induction of SlSOS1 expression, while the higher K
accumulation in roots seems to be mainly determined by expression of LKT1 transporter and SlSKOR channel. These findings demonstrate that SlCBL10 is a negative regulator of salt tolerance in the root, so the use of downregulated SlCBL10 rootstocks may provide a suitable strategy to increase tomato fruit production under salinity.
A dramatic evolution of fruit size has accompanied the domestication and improvement of fruit-bearing crop species. In tomato (Solanum lycopersicum), naturally occurring cis-regulatory mutations in ...the genes of the CLAVATA-WUSCHEL signaling pathway have led to a significant increase in fruit size generating enlarged meristems that lead to flowers with extra organs and bigger fruits. In this work, by combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBER OF FLORAL ORGANS (ENO), an AP2/ERF transcription factor which regulates floral meristem activity. Thus, the ENO gene mutation gives rise to plants that yield larger multilocular fruits due to an increased size of the floral meristem. Genetic analyses indicate that eno exhibits synergistic effects with mutations at the LOCULE NUMBER (encoding SlWUS) and FASCIATED (encoding SlCLV3) loci, two central players in the evolution of fruit size in the domestication of cultivated tomatoes. Our findings reveal that an eno mutation causes a substantial expansion of SlWUS expression domains in a flower-specific manner. In vitro binding results show that ENO is able to interact with the GGC-box cis-regulatory element within the SlWUS promoter region, suggesting that ENO directly regulates SlWUS expression domains to maintain floral stem-cell homeostasis. Furthermore, the study of natural allelic variation of the ENO locus proved that a cis-regulatory mutation in the promoter of ENO had been targeted by positive selection during the domestication process, setting up the background for significant increases in fruit locule number and fruit size in modern tomatoes.
Ca
2+
is a second messenger that mediates plant responses to abiotic stress; Ca
2+
signals need to be decoded by Ca
2+
sensors that translate the signal into physiological, metabolic, and molecular ...responses. Recent research regarding the Ca
2+
sensor CALCINEURIN B-LIKE PROTEIN 10 (CBL10) has resulted in important advances in understanding the function of this signaling component during abiotic stress tolerance. Under saline conditions, CBL10 function was initially understood to be linked to regulation of Na
+
homeostasis, protecting plant shoots from salt stress. During this process, CBL10 interacts with the CBL-interacting protein kinase 24 (CIPK24, SOS2), this interaction being localized at both the plasma and vacuolar (tonoplast) membranes. Interestingly, recent studies have exposed that CBL10 is a regulator not only of Na
+
homeostasis but also of Ca
2+
under salt stress, regulating Ca
2+
fluxes in vacuoles, and also at the plasma membrane. This review summarizes new research regarding functions of CBL10 in plant stress tolerance, predominantly salt stress, as this is the most commonly studied abiotic stress associated with the function of this regulator. Special focus has been placed on some aspects that are still unclear. We also pay particular attention on the proven versatility of CBL10 to activate (in a CIPK-dependent manner) or repress (by direct interaction) downstream targets, in different subcellular locations. These in turn appear to be the link through which CBL10 could be a key master regulator of stress signaling in plants and also a crucial participant in fruit development and quality, as disruption of
CBL10
results in inadequate Ca
2+
partitioning in plants and fruit. New emerging roles associated with other abiotic stresses in addition to salt stress, such as drought, flooding, and K
+
deficiency, are also addressed in this review. Finally, we provide an outline of recent advances in identification of potential targets of CBL10, as CBL10/CIPKs complexes and as CBL10 direct interactions. The aim is to showcase new research regarding this master regulator of abiotic stress tolerance that may be essential to the maintenance of crop productivity under abiotic stress. This is particularly pertinent when considering the scenario of a projected increase in extreme environmental conditions due to climate change.
Summary
CRABS CLAW (CRC) orthologues play a crucial role in floral meristem (FM) determinacy and gynoecium formation across angiosperms, the key developmental processes for ensuring successful plant ...reproduction and crop production. However, the mechanisms behind CRC mediated FM termination are far from fully understood.
Here, we addressed the functional characterization of tomato (Solanum lycopersicum) paralogous CRC genes. Using mapping‐by‐sequencing, RNA interference and CRISPR/Cas9 techniques, expression analyses, protein–protein interaction assays and Arabidopsis complementation experiments, we examined their potential roles in FM determinacy and carpel formation.
We revealed that the incomplete penetrance and variable expressivity of the indeterminate carpel‐inside‐carpel phenotype observed in fruit iterative growth (fig) mutant plants are due to the lack of function of the S. lycopersicum CRC homologue SlCRCa. Furthermore, a detailed functional analysis of tomato CRC paralogues, SlCRCa and SlCRCb, allowed us to propose that they operate as positive regulators of FM determinacy by acting in a compensatory and partially redundant manner to safeguard the proper formation of flowers and fruits.
Our results uncover for the first time the physical interaction of putative CRC orthologues with members of the chromatin remodelling complex that epigenetically represses WUSCHEL expression through histone deacetylation to ensure the proper termination of floral stem cell activity.
Pollination and therefore fruit set in tomato (Solanum lycopersicum) is very sensitive to temperature. Parthenocarpy can be a very useful trait in tomato breeding in sustainable agriculture faced ...with global warming. Within a collection of Moneymaker tomato mutants a monogenic and recessive mutant with high fruit number was identified in heat stress conditions and named high fruit set under stress (hfs). No morphological alterations in vegetative and reproductive organs were observed except the bigger size of ovary. hfs is parthenocarpic, pollination not being required for fruit set, although this trait was not absolute since it produced some under‐seeded fruit. When plants were grown under extreme temperatures (higher than 35°C), hfs exhibits higher fruit yield than wild‐type (WT) due to increased fruit number. Another very interesting characteristic of hfs is its improved fruit quality under heat stress, exhibiting a better sweetness/acidity balance than WT. Interestingly, hfs was also tolerant to the combination of heat and salt stress, and the positive effect on production was due to both components of yield, fruit number and fruit weight. The generation of mostly seedless fruit and the high productivity and fruit quality under extreme temperatures make hfs a very interesting mutant to obtain new breeding high‐yield lines in adverse environmental conditions.
A new identified monogenic and recessive parthenocarpic tomato mutant (high fruit set under stress hfs) that exhibits high fruit productivity in heat (H; >35°C) and salt (S; 100 mM NaCl) stress and their combination (H + S), compared with wild‐type (C: controlled unstressed conditions). hfs shows no morphological alterations in vegetative and reproductive development but mutant fruit presents higher fruit quality (total soluble solids and titratable acidity).
Tomato cell wall-associated kinase 1 (
) has only been studied in biotic stress response and hence its function in abiotic stress remains unknown. In a screening under salinity of an insertional ...mutant collection of tomato (
L.), a mutant exhibiting lower degree of leaf chlorosis than wild type (WT) together with reduced leaf Na
accumulation was selected. Genetic analysis of the mutation revealed that a single T-DNA insertion in the
gene was responsible of the mutant phenotype.
null mutant reduced its shoot growth compared with WT, despite its improved Na
homeostasis.
disruption affected osmotic homeostasis, as leaf water content was lower in mutant than in WT under salt stress. In addition,
altered the source-sink balance under salinity, by increasing sucrose content in roots. Finally, a significant fruit yield reduction was found in
vs. WT under long-term salt stress, mainly due to lower fruit weight. Our results show that disruption of
induces a higher sucrose transport from source leaf to sink root, negatively affecting fruit, the main sink at adult stage.
Summary
With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are ...based on the analysis of phenotypic variations between a wild type and its mutant; hence, mutant collections are a valuable resource. In this sense, T‐DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T‐DNA collection in tomato (Solanum lycopersicum L.), which has been produced by Agrobacterium‐mediated transformation using a binary vector bearing a minimal promoter fused to the uidA reporter gene. Two genes have been isolated from different T‐DNA mutants, one of these genes codes for a UTP‐glucose‐1‐phosphate uridylyltransferase involved in programmed cell death and leaf development, which means a novel gene function reported in tomato. Together, our results support that enhancer trapping is a powerful tool to identify novel genes and regulatory elements in tomato and that this T‐DNA mutant collection represents a highly valuable resource for functional analyses in this fleshy‐fruited model species.
Characterization of a new tomato (Solanum lycopersicum) T-DNA mutant allowed for the isolation of the CALCINEURIN B-LIKE PROTEIN 10 (SlCBL10) gene whose lack of function was responsible for the ...severe alterations observed in the shoot apex and reproductive organs under salinity conditions. Physiological studies proved that SlCBL10 gene is required to maintain a proper low Na⁺/Ca2⁺ ratio in growing tissues allowing tomato growth under salt stress. Expression analysis of the main responsible genes for Na⁺ compartmentalization (i.e. Na⁺/H⁺ EXCHANGERs, SALT OVERLY SENSITIVE, HIGH-AFFINITY K+ TRANSPORTER 1;2, H⁺-pyrophosphatase AVP1 SlAVP1 and V-ATPase SlVHA-A1) supported a reduced capacity to accumulate Na⁺ in Slcbl10 mutant leaves, which resulted in a lower uploading of Na⁺ from xylem, allowing the toxic ion to reach apex and flowers. Likewise, the tomato CATION EXCHANGER 1 and TWO-PORE CHANNEL 1 (SlTPC1), key genes for Ca2⁺ fluxes to the vacuole, showed abnormal expression in Slcbl10 plants indicating an impaired Ca2⁺ release from vacuole. Additionally, complementation assay revealed that SlCBL10 is a true ortholog of the Arabidopsis (Arabidopsis thaliana) CBL10 gene, supporting that the essential function of CBL10 is conserved in Arabidopsis and tomato. Together, the findings obtained in this study provide new insights into the function of SlCBL10 in salt stress tolerance. Thus, it is proposed that SlCBL10 mediates salt tolerance by regulating Na⁺ and Ca2⁺ fluxes in the vacuole, cooperating with the vacuolar cation channel SlTPC1 and the two vacuolar H⁺-pumps, SlAVP1 and SlVHA-A1, which in turn are revealed as potential targets of SlCBL10.
Floral organogenesis requires coordinated interactions between genes specifying floral organ identity and those regulating growth and size of developing floral organs. With the aim to isolate ...regulatory genes linking both developmental processes (i.e., floral organ identity and growth) in the tomato model species, a novel mutant altered in the formation of floral organs was further characterized. Under normal growth conditions, floral organ primordia of mutant plants were correctly initiated, however, they were unable to complete their development impeding the formation of mature and fertile flowers. Thus, the growth of floral buds was blocked at an early stage of development; therefore, we named this mutant as
(
). Genetic analysis performed in a segregating population of 543 plants showed that the abnormal phenotype was controlled by a single recessive mutation. Global gene expression analysis confirmed that several MADS-box genes regulating floral identity as well as other genes participating in cell division and different hormonal pathways were affected in their expression patterns in
mutant plants. Moreover,
mutant inflorescences showed higher hormone contents, particularly ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and strigol compared to wild type. Such results indicate that
may have a key function as positive regulator of the development of floral primordia once they have been initiated in the four floral whorls. This function should be performed by affecting the expression of floral organ identity and growth genes, together with hormonal signaling pathways.
The tropical common bean (
L.) is an obligatory short-day plant that requires relaxation of the photoperiod to induce flowering. Similar to other crops, photoperiod-induced floral initiation depends ...on the differentiation and maintenance of meristems. In this study, the global changes in transcript expression profiles were analyzed in two meristematic tissues corresponding to the vegetative and inflorescence meristems of two genotypes with different sensitivities to photoperiods. A total of 3396 differentially expressed genes (DEGs) were identified, and 1271 and 1533 were found to be up-regulated and down-regulated, respectively, whereas 592 genes showed discordant expression patterns between both genotypes.
homologues of DEGs were identified, and most of them were not previously involved in
floral transition, suggesting an evolutionary divergence of the transcriptional regulatory networks of the flowering process of both species. However, some genes belonging to the photoperiod and flower development pathways with evolutionarily conserved transcriptional profiles have been found. In addition, the flower meristem identity genes
and
, as well as
, were identified as markers to distinguish between the vegetative and reproductive stages. Our data also indicated that the down-regulation of the photoperiodic genes seems to be directly associated with promoting floral transition under inductive short-day lengths. These findings provide valuable insight into the molecular factors that underlie meristematic development and contribute to understanding the photoperiod adaptation in the common bean.
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