Flowering is a critical agricultural trait that substantially affects tomato fruit yield. Although drought stress influences flowering time, the molecular mechanism underlying drought-regulated ...flowering in tomato remains elusive. In this study, we demonstrated that loss of function of tomato OPEN STOMATA 1 (SlOST1), a protein kinase essential for abscisic acid (ABA) signaling and abiotic stress responses, lowers the tolerance of tomato plants to drought stress. slost1 mutants also exhibited a late flowering phenotype under both normal and drought stress conditions. We also established that SlOST1 directly interacts with and phosphorylates the NAC (NAM, ATAF and CUC)-type transcription factor VASCULAR PLANT ONE-ZINC FINGER 1 (SlVOZ1), at residue serine 67, thereby enhancing its stability and nuclear translocation in an ABA-dependent manner. Moreover, we uncovered several SlVOZ1 binding motifs from DNA affinity purification sequencing analyses and revealed that SlVOZ1 can directly bind to the promoter of the major flowering-integrator gene SINGLE FLOWER TRUSS to promote tomato flowering transition in response to drought. Collectively, our data uncover the essential role of the SlOST1-SlVOZ1 module in regulating flowering in response to drought stress in tomato and offer insights into a novel strategy to balance drought stress response and flowering.
As an evolutionarily conserved multi-protein complex, the Mediator complex modulates the association between transcription factors and RNA polymerase II to precisely regulate gene transcription. ...Although numerous studies have shown the diverse functions of Mediator complex in plant development, flowering, hormone signaling, and biotic stress response, its roles in the Abscisic acid (ABA) signaling pathway and abiotic stress response remain largely unclear. It has been recognized that the phytohormone, ABA, plays a predominant role in regulating plant adaption to various abiotic stresses as ABA can trigger extensive changes in the transcriptome to help the plants respond to environmental stimuli. Over the past decade, the Mediator complex has been revealed to play key roles in not only regulating the ABA signaling transduction but also in the abiotic stress responses. In this review, we will summarize current knowledge of the Mediator complex in regulating the plants' response to ABA as well as to the abiotic stresses of cold, drought and high salinity. We will particularly emphasize the involvement of multi-functional subunits of MED25, MED18, MED16, and CDK8 in response to ABA and environmental perturbation. Additionally, we will discuss potential research directions available for further deciphering the role of Mediator complex in regulating ABA and other abiotic stress responses.
Hydropower units are the core equipment of hydropower stations, and research on the fault prediction and health management of these units can help improve their safety, stability, and the level of ...reliable operation and can effectively reduce costs. Therefore, it is necessary to predict the swing trend of these units. Firstly, this study considers the influence of various factors, such as electrical, mechanical, and hydraulic swing factors, on the swing signal of the main guide bearing y-axis. Before swing trend prediction, the multi-index feature selection algorithm is used to obtain suitable state variables, and the low-dimensional effective feature subset is obtained using the Pearson correlation coefficient and distance correlation coefficient algorithms. Secondly, the dilated convolution graph neural network (DCGNN) algorithm, with a dilated convolution graph, is used to predict the swing trend of the main guide bearing. Existing GNN methods rely heavily on predefined graph structures for prediction. The DCGNN algorithm can solve the problem of spatial dependence between variables without defining the graph structure and provides the adjacency matrix of the graph learning layer simulation, avoiding the over-smoothing problem often seen in graph convolutional networks; furthermore, it effectively improves the prediction accuracy. The experimental results showed that, compared with the RNN-GRU, LSTNet, and TAP-LSTM algorithms, the MAEs of the DCGNN algorithm decreased by 6.05%, 6.32%, and 3.04%; the RMSEs decreased by 9.21%, 9.01%, and 2.83%; and the CORR values increased by 0.63%, 1.05%, and 0.37%, respectively. Thus, the prediction accuracy was effectively improved.
During the traditional reverse electrodialysis (RED) process, second only to concentration polarization phenomenon, the spacer shadow effect that blocks the ionic transport as a consequence of the ...use of non-permselective spacers has an undesirable impact on the practical power output. In this work, a configuration of ion-permselective spacer is proposed to eliminate the spacer shadow effect to improve the power density. By combining direct current and alternating current experimental methods, the spacer shadow and concentration polarization effects are measured. At the same time, their impact on stack resistance and power out are analyzed quantatively under different hydrodynamic conditions and spacer configurations to optimize the RED process performance. Compared with traditional spacers, the use of ion-permselective spacers makes the spacer shadow effect reduced by more than 95% inversely with a stronger concentration polarization phenomenon, which results in a higher power density due to remarkable decrease in stack resistance. In order to further suppress the concentration polarization, increasing flow rate and reducing pore size of channel are both feasible, which meanwhile leads to a further reduction in shadow effect. Yet, the variation of channel shape has little impact on the shadow and concentration polarization effects. The maximum power output of 0.81 W/m
2
is harvested with pore size of 4.5 mm and cell pair of 3 at flow rate of 40 L/h under the investigated conditions and is improved by 50% in comparison to non-permselective spacers.
Graphical abstract
Rotor-stator interaction in axial pumps can produce pressure fluctuations and further vibrations even damage to the pump system in some extreme case. In this paper, the influence of tip clearance on ...pressure fluctuations in an axial flow water pump has been investigated by numerical method. Three-dimensional unsteady flow in the axial flow water pump has been simulated with different tip clearances between the impeller blade tip and the casing wall. In addition to monitoring pressure fluctuations at some typical points, a new method based on pressure statistics was proposed to determine pressure fluctuations at all grid nodes inside the whole pump. The comparison shows that the existence of impeller tip clearance magnifies the pressure fluctuations in the impeller region, from the hub to shroud. However, the effect on pressure fluctuation in the diffuser region is not evident. Furthermore, the tip clearance vortex has also been examined under different tip clearances.
Precessing vortex rope (PVR) plays a key role in inducing hydraulic resonance in Francis turbines operating at partial load, possibly degrading power plant stability and availability. Air injection ...into the runner cone is a suitable mitigating alternative; however, the influence mechanism of air injection on PVR remains unclear. The principal objective of this study was to establish response relationships between the characteristic parameters and air injection by the method of computational fluid dynamics (CFD) considering the fluid components of water, water vapour and air. The findings show that cavitation flow can be completely suppressed by slight air injection; however, the helical vortex structures are persisted at 1.0% and 2.0% air volume fractions, and the static pressure recovery is improved together with a slight increase in the hydraulic loss. At 3.0% air volume fraction, the vortex structure completely disappears, leaving an umbrella-shaped structure, with no pressure vibration arising in the turbine. Moreover, the physical mechanism of reducing the pressure amplitudes is clarified. This results clarify the influence mechanism of air injection on PVR, and contribute to steadily extending the flexibility of the operating range of the turbine during the engineering application.
Apoptosis of chondrocyte is involved in osteoarthritis (OA) pathogenesis, and mechanical stress plays a key role in this process by activation of Piezo1. However, the negative regulation of signal ...conduction mediated by mechanical stress is still unclear. Here, we elucidate that the critical role of G protein coupled estrogen receptor (GPER) in the regulation of mechanical stress-mediated signal transduction and chondrocyte apoptosis.
The gene expression profile was detected by gene chip upon silencing Piezo1. The expression of GPER in cartilage tissue taken from the clinical patients was detected by RT-PCR and Western blot as well as immunohistochemistry, and the correlation between GPER expression and OA was also investigated. The chondrocytes exposed to mechanical stress were treated with estrogen, G-1, G15, GPER-siRNA and YAP (Yes-associated protein)-siRNA. The cell viability of chondrocytes was measured. The expression of polymerized actin and Piezo1 as well as the subcellular localization of YAP was observed under laser confocal microscope. Western blot confirmed the changes of YAP/ Rho GTPase activating protein 29 (ARHGAP29) /RhoA/LIMK /Cofilin pathway. The knee specimens of osteoarthritis model were stained with safranin and green. OARSI score was used to evaluate the joint lesions. The expressions of GPER and YAP were detected by immunochemistry.
Expression profiles of Piezo1- silenced chondrocytes showed that GPER expression was significantly upregulated. Moreover, GPER was negatively correlated with cartilage degeneration during OA pathogenesis. In addition, we uncovered that GPER directly targeted YAP and broadly restrained mechanical stress-triggered actin polymerization. Mechanism studies revealed that GPER inhibited mechanical stress-mediated RhoA/LIMK/cofilin pathway, as well as the actin polymerization, by promoting expression of YAP and ARHGAP29, and the YAP nuclear localization, eventually causing the inhibition of Piezo1. YAP was obviously decreased in degenerated cartilage. Silencing YAP caused significantly increased actin polymerization and activation of Piezo1, and an increase of chondrocyte apoptosis. In addition, intra-articular injection of G-1 to OA rat effectively attenuated cartilage degeneration.
We propose a novel regulatory mechanism underlying mechanical stress-mediated apoptosis of chondrocyte and elucidate the potential application value of GPER as therapy targets for OA.
The internal flow scale of a Pelton turbine is variable, and the interaction between the jet and the bucket has strong transient characteristics, resulting in an incomplete understanding of its ...internal vortex structure evolution and energy dissipation mechanisms. In order to reveal the influence of vortices on the flow regime and energy dissipation mechanisms in the turbine, this paper establishes the correlation between the unsteady flow characteristics and energy dissipation inside the Pelton turbine based on the energy balance equation and quantifies the energy losses inside the turbine. The results indicate that vortex structures present a non-uniform distribution inside the jet, which disrupts the uniformity of the jet velocity distribution, resulting in an uneven distribution of high-vorticity zones on the bucket surfaces and intensifying flow interference among the buckets. The strong shear flow caused by the downstream flow detachment of the needle guide, the turbulent boundary layer on the jet surface, and the wake effect downstream of the needle tip are the main reasons for the dissipation of fluid kinetic energy. The distribution range of energy loss on the rotating bucket closely corresponds to the position of the high-vorticity zone. Energy dissipation inside the turbine is primarily in the form of turbulent kinetic energy, and the injectors and runner are the primary energy dissipation components. Moreover, inside the injectors, each form of energy loss remains relatively constant, whereas inside the runner, its rotation induces fluctuating energy losses attributed to Reynolds stress work. The results contribute to an enhanced understanding of the energy dissipation characteristics and complex flow mechanisms within the turbine, providing reference for the optimisation and efficient operation of the multi-nozzle Pelton turbine.
The massive grid integration of renewable energy necessitates frequent and rapid response of hydropower output, which has brought enormous challenges to the hydropower operation and new opportunities ...for hydropower development. To investigate feasible solutions for complementary systems to cope with the energy transition in the context of the constantly changing role of the hydropower plant and the rapid evolution of wind and solar power, the short-term coordinated scheduling model is developed for the wind–solar–hydro hybrid pumped storage (WSHPS) system with peak shaving operation. The effects of different reservoir inflow conditions, different wind and solar power forecast output, and installed capacity of pumping station on the performance of WSHPS system are analyzed. The results show that compared with the wind–solar–hydro hybrid (WSH) system, the total power generation of the WSHPS system in the dry, normal, and wet year increased by 10.69%, 11.40%, and 11.27% respectively. The solar curtailment decreased by 68.97%, 61.61%, and 48.43%, respectively, and the wind curtailment decreased by 76.14%, 58.48%, and 50.91%, respectively. The high proportion of wind and solar energy connected to the grid in summer leads to large net load fluctuations and serious energy curtailment. The increase in the installed capacity of the pumping station will promote the consumption of wind and solar energy in the WSHPS system. The model proposed in this paper can improve the operational flexibility of hydropower station and promote the consumption of wind and solar energy, which provides a reference for the research of cascade hydropower energy storage system.
Inter‐blade cavitation vortex at part load has raised significant concerns about operation stability for Francis turbines due to tremendous development and integration of renewable energy resources ...into power grid. The principal objective of this study is to investigate the flow characteristics of inter‐blade cavitation vortex and its influence on pressure fluctuations for a low‐head Francis model turbine. Unsteady numerical simulations are carried out using shear stress transport turbulence model and Zwart cavitation model. Numerical results yield to a good validation with the experimental data. The findings show that inter‐blade cavitation vortices highlighted by cavitation structure and vortex structure are extremely different at the points adjacent to the incipient line of inter‐blade cavitation vortex. The location of inter‐blade cavitation vortex development in the runner is more sensitive to rotating speed than that to guide vane opening. The formation mechanism of inter‐blade cavitation vortex is attributed to flow separation in the vicinity of the runner hub. Pressure fluctuations induced by inter‐blade cavitation vortex are directly associated with the evolution of vapour volume in the runner and feature wide‐band and low‐frequency characteristic, and the fluctuations on the suction side of the runner blade are significantly magnified because of the presence of inter‐blade cavitation vortex.
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