Lymphocytes express tyrosine hydroxylase (TH), the rate-limiting enzyme for the synthesis of dopamine, norepinephrine and epinephrine. This suggests a broader role for cathecholamines in lymphocyte ...function, as well as the potential secretion of catecholamines by tumors of lymphoid origin. Our aim was to evaluate the expression of Th by murine lymphoma cells in an in vivo mouse model. For this, L5178Y-R lymphoma cells were implanted in nerve-intact and sympathectomized male BALB/c mice. Relative Th gene expression in tumor and brain was determined by quantitative PCR. Body composition, tumor volume, and plasma TH1/TH2/TH17 cytokines were also evaluated as markers of tumor-host condition and anti-tumor immune response in absence of adrenergic innervation.
We found a significant (p = 0.045) 3.3-fold decrease of Th gene expression in tumor and a non-significant (p = 0.60) 6.9-fold increase in brain after sympathectomy. Sympathectomized mice also showed a significant increase in tumor mass at days 18 (p = 0.032) and 28 (p = 0.022) and increased interscapular fat (p = 0.04). TH1/TH2 and TH17 cytokines levels in plasma from sympathectomized tumor-bearing mice were not different from control mice.
The L5178Y-R lymphoma does not express Th during in vivo progression.
Bacteria of the SAR11 clade constitute up to one half of all microbial cells in the oxygen-rich surface ocean. SAR11 bacteria are also abundant in oxygen minimum zones (OMZs), where oxygen falls ...below detection and anaerobic microbes have vital roles in converting bioavailable nitrogen to N2 gas. Anaerobic metabolism has not yet been observed in SAR11, and it remains unknown how these bacteria contribute to OMZ biogeochemical cycling. Here, genomic analysis of single cells from the world's largest OMZ revealed previously uncharacterized SAR11 lineages with adaptations for life without oxygen, including genes for respiratory nitrate reductases (Nar). SAR11 nar genes were experimentally verified to encode proteins catalysing the nitrite-producing first step of denitrification and constituted ~40% of OMZ nar transcripts, with transcription peaking in the anoxic zone of maximum nitrate reduction activity. These results link SAR11 to pathways of ocean nitrogen loss, redefining the ecological niche of Earth's most abundant organismal group.
•Cardiovascular disease tops maternal mortality causes in high-income countries.•International experts suggest educational curricula in obstetric critical care.•Key critical care topics such as ...advanced ultrasonography are warranted.•Critical care echocardiography allows advanced assessment of high-risk patients.
Globally, the increase in medically complex obstetric patients is challenging the educational approach and clinical management of critically ill obstetric patients. This increase in medical complexity calls into question the educational paradigm in which future physicians are trained. Obstetric anesthesiologists, physician experts in the perio-perative planning and management of complex obstetric patients, represent an essential workforce in the strategies to address maternal mortality. Unfortunately, the development of peri-operative medicine and maternal critical care curricula has only received minor attention in most countries.
Proposed guidelines and models highlight the existing need for tiered maternity care services in which critical care infrastructure plays a central role in the delivery of high-risk peripartum care. Therefore, the development of maternal critical care models designed to prepare obstetric anesthesiologists for the clinical challenges of a medically complex patient are warranted. Key critical care topics such as advanced ultrasonography, with the inclusion of quantitative echocardiographic assessments into obstetric anesthesiology educational curricula, will serve to better prepare physicians for the realities of an increasingly complex pregnant patient population, and further reinforce the critical care infrastructure detailed in the Levels of Maternal Care consensus.
Despite an increasingly complex obstetric patient population, heterogeneity of maternal critical care practices exists across the globe, warranting standardization and further development of proposed curricula.
The Achilles tendon is the strongest tendon in the human body but its mechanical behaviour during failure has been little studied and the basis of its high tensile strength has not been elucidated in ...detail. In the present study, healthy, human, Achilles tendons were loaded to failure in an anatomically authentic fashion while the local deformation and strains were studied in real time, with very high precision, using digital image correlation (DIC). The values determined for the strength of the Achilles tendon were at the high end of those reported in the literature, consistent with the absence of a pre-existing tendinopathy in the samples, as determined by careful gross inspection and histology. Early in the loading cycle, the proximal region of the tendon accumulated high lateral strains while longitudinal strains remained low. However, immediately before rupture, the mid-substance of the Achilles tendon, its weakest part, started to show high longitudinal strains. These new insights advance the understanding of the mechanical behaviour of tendons as they are stretched to failure.
Summary
Marinimicrobia bacteria are widespread in subeuphotic areas of the oceans and particularly abundant in oxygen minimum zones (OMZs). Information on Marinimicrobia metabolism is sparse, making ...the biogeochemical influence of this group challenging to predict. Here, metagenome‐assembled genomes representing Marinimicrobia subgroups PN262000N21 and ARCTIC96B‐7 were retrieved to near completion (97% and 94%) from OMZ metagenomes, with contamination (14.1%) observed only in ARCTIC96B‐7. Genes for aerobic carbon monoxide (CO) oxidation, polysulfide metabolism and hydrogen utilization were identified only in PN262000N21, while genes for partial denitrification occurred in both genomes. Transcripts mapping to these genomes increased from <0.3% of total mRNA from the oxic zone to a max of 22% under anoxia. ARCTIC96B‐7 transcript representation decreased an order of magnitude from non‐sulfidic to sulfidic depths. In contrast, PN262000N21 representation was relatively constant throughout the OMZ, although transcripts encoding sulfur‐utilizing proteins, including sulfur transferases, were enriched at sulfidic depths. PN262000N21 transcripts encoding a protein with fibronectin domains similar to those in cellulosome‐producing bacteria were also abundant, suggesting a potential for high molecular weight carbon cycling. These data provide omic‐level descriptions of metabolic potential and activity in OMZ‐associated Marinimicrobia, suggesting differentiation between subgroups with roles in carbon and dissimilatory inorganic nitrogen and sulfur cycling.
Abstract
The Physics of the Accelerating Universe Survey (PAUS) is an innovative photometric survey with 40 narrow-bands at the William Herschel Telescope (WHT). The narrow-bands are spaced at 100 Å ...intervals covering the range 4500–8500 Å and, in combination with standard broad-bands, enable excellent redshift precision. This paper describes the technique, galaxy templates, and additional photometric calibration used to determine early photometric redshifts from PAUS. Using bcnz2, a new photometric redshift code developed for this purpose, we characterize the photometric redshift performance using PAUS data on the COSMOS field. Comparison to secure spectra from zCOSMOS DR3 shows that PAUS achieves σ68/(1 + $z$) = 0.0037 to iAB < 22.5 for the redshift range 0 < $z$ < 1.2, when selecting the best 50 per cent of the sources based on a photometric redshift quality cut. Furthermore, a higher photo-z precision σ68/(1 + $z$) ∼ 0.001 is obtained for a bright and high-quality selection, which is driven by the identification of emission lines. We conclude that PAUS meets its design goals, opening up a hitherto uncharted regime of deep, wide, and dense galaxy survey with precise redshifts that will provide unique insights into the formation, evolution, and clustering of galaxies, as well as their intrinsic alignments.
Rodent models are commonly used to investigate tendon healing, with the biomechanical and structural properties of the healed tendons being important outcome measures. Tendon storage for later ...testing becomes necessary when performing large experiments with multiple time-points. However, it is unclear whether freezing rodent tendons affects their material properties. Thus the aim of this study was to determine whether freezing rat Achilles tendons affects their biomechanical or structural properties. Tendons were frozen at either −20 °C or −80 °C directly after harvesting, or tested when freshly harvested. Groups of tendons were subjected to several freeze-thaw cycles (1, 2, and 5) within 3 months, or frozen for 9 months, after which the tendons were subjected to biomechanical testing. Additionally, fresh and thawed tendons were compared morphologically, histologically and by transmission electron microscopy. No major differences in biomechanical properties were found between fresh tendons and those frozen once or twice at −20 °C or −80 °C. However, deterioration of tendon properties was found for 5-cycle groups and both long-term freezing groups; after 9 months of freezing at −80 °C the tear resistance of the tendon was reduced from 125.4 ± 16.4N to 74.3 ± 18.4N (p = 0.0132). Moreover, tendons stored under these conditions showed major disruption of collagen fibrils when examined by transmission electron microscopy. When examined histologically, fresh samples exhibited the best cellularity and proteoglycan content of the enthesis. These properties were preserved better after freezing at −80 °C than after freezing at −20 °C, which resulted in markedly smaller chondrocytes and less proteoglycan content. Overall, the best preservation of histological integrity was seen with tendons frozen once at −80 °C. In conclusion, rat Achilles tendons can be frozen once or twice for short periods of time (up to 3 months) at −20 °C or −80 °C for later testing. However, freezing for 9 months at either −20 °C or −80 °C leads to deterioration of certain parameters.
In this study, the electro-oxidation reaction of ethanol over Pd–Cu supported on Cu porphyrin (TPPCu) was investigated. The catalyst was synthesized using the microwave-assisted polyol method and ...physicochemically characterized by XRD, XPS, SEM, EDS, TEM, EDAX, UV–Vis, FTIR, and RBS. A Cu-enriched catalyst with Cu
3
Pd, Pd,Cu, and TPPCu phases was identified using XRD and XPS. However, according to the RBS results, the catalytic surface was enriched with Pd, indicating that the interaction between TPPCu and Pd–Cu allowed the presence of Pd on the surface, thus enhancing the catalytic response of the material. This synthesis prevented the deprotonation of porphyrin on the electrocatalyst, as confirmed by XPS analysis. Electrochemical studies based on cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the response of the catalyst to variations in the scan rate and increasing ethanol concentration. The electrochemical response of PdCu/TPPCu improved with an increasing number of cycles, indicating improved mass transport, thus improving its electrochemical response and tolerance to CO contamination. This catalyst exhibited a high electroactive surface area of 49.4 m
2
/g, which could be related to the presence of TPPCu as a support. The behavior of the catalyst on the anode of a fuel cell fed with ethanol, bioethanol, and bioethanol residues was evaluated.
Graphical Abstract
Tendon injuries present a considerable clinical challenge due to the limited regenerative capacity of tendons. The use of gene transfer to deliver growth factors to sites of tendon damage has been ...suggested as a promising strategy for improving tendon regeneration. A major issue for this approach is to identify clinically acceptable vectors that can deliver genes to the cells of the tendon, preferably by in vivo delivery. Adeno-associated virus (AAV) has many advantages in this regard, including a favourable safety profile and the ability to sustain long-term transgene expression. Here we explored the use of AAV to deliver marker genes to the supra- and infra-spinatus tendons of the rotator cuff in the rat by injection into the subacromial space. First, we screened various AAV serotypes for their transducing ability towards rat and human tenocytes in vitro. Of the 10 serotypes tested, AAV2.5 and AAV2 exhibited the highest in vitro transduction efficiency in both rat and human tenocytes. Ex vivo transduction of cells within explants of isolated, intact tendon was also demonstrated. Injection of AAV2.5 encoding luciferase into the subacromial space confirmed gene delivery to the infra-, but not supra-, spinatus tendon in vivo with transgene expression persisting for 7 days post-transduction. These data demonstrate the ability of AAV2.5 to deliver genes to the infraspinatus tendon, leading to sustained local expression following in vivo delivery. Our findings suggest that AAV2.5 has several advantages as vector for stimulating tendon regeneration by local, in vivo, gene transfer.
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