Purpose:
This study aimed to determine whether insomnia is associated with hypertension (HBP) and coronary artery disease (CAD) in a hospital-based sample of patients with type 2 diabetes mellitus ...(T2DM).
Methods:
Our present study included 354 patients with T2DM. According to the diagnostic criteria of insomnia, the participants were assigned to three groups based on the duration of T2DM and insomnia diagnosis. Patients with T2DM alone were placed in group A; patients with T2DM longer than insomnia were placed in group B; and patients with insomnia longer than T2DM were placed in group C. Medical history was collected from all the patients in detail. Besides, the participants underwent thorough physical examinations and laboratory measurements. Propensity score matching (PSM) was applied to evaluate the associations of insomnia with HBP and CAD. The univariate and multivariate logistic regression analysis was used to explore whether insomnia was a risk factor for HBP and CAD in patients with T2DM.
Results:
Of 354 patients, 225 patients were included in group A, 62 patients were included in group B, and 67 patients were included in group C. Compared with groups B and C, group A showed a lower prevalence of HBP and CAD (
p
< 0.05). In addition, compared with group B, group C showed no difference in the prevalence of HBP and CAD (
p
> 0.05). After PSM was performed, groups B and C had a higher prevalence of HBP and CAD (
p
< 0.05) than group A with no significant difference between groups B and C (
p
> 0.05). In the univariate and multivariate logistic regression analysis, insomnia was a risk factor for HBP univariate: odds ratio (OR) = 3.376, 95% CI 2.290–6.093,
p
< 0.001; multivariate: OR = 2.832, 95% CI 1.373–5.841,
p
= 0.005 and CAD (univariate: OR = 5.019, 95% CI 3.148–8.001,
p
< 0.001; multivariate: OR = 5.289, 95% CI 2.579–10.850,
p
< 0.001).
Conclusion:
T2DM combined with insomnia was related to HBP and CAD and insomnia was a risk factor for HBP and CAD in patients with T2DM. However, larger, prospective studies are required to confirm our findings.
Alpinia officinarum Hance (A. officinarum), a perennial herb known for its medicinal properties, has been used to treat various ailments, such as stomach pain, abdominal pain, emesis, and digestive ...system cancers. A. officinarum is extensively cultivated in the Qiongzhong and Baisha regions of Hainan, and it holds substantial therapeutic value for the local Li people of Hainan. Kaempferol, a flavonoid derived from A. officinarum, has demonstrated anticancer properties in various experimental and biological studies. Nevertheless, the precise mechanisms through which it exerts its anti-hepatocellular carcinoma (HCC) effects remain to be comprehensively delineated.
This investigation aims to elucidate the anti-HCC effects of kaempferol derived from A. officinarum and to delve into its underlying mechanistic pathways.
Using ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS) to identify active compounds in A. officinarum. HCCLM3 and Huh7 cells were used to study the anti-HCC effect of kaempferol from A. officinarum. The cytotoxicity and proliferation of kaempferol and A. officinarum were measured using CCK-8 and EDU staining. Wound-healing assays and three-dimensional tumor spheroid models were further used to evaluate migration and the anti-HCC activity of kaempferol. The cell cycle and apoptosis were evaluated by flow cytometry. Western blot and qRT-PCR were used to detect the expression of proteins and genes associated with the cell cycle checkpoints. Finally, bioinformatics was used to analyze the relationship between the differential expression of core targets in the ATM/CHEK2/KNL1 pathway and a poor prognosis in clinical HCC samples.
UPLC-MS/MS was employed to detect five active compounds in A. officinarum, such as kaempferol. The CCK-8 and EDU assays showed that kaempferol and A. officinarum significantly inhibited the proliferation of HCC cells. A wound-healing assay revealed that kaempferol remarkably inhibited the migration of HCC cells. Kaempferol significantly suppressed the growth of tumor spheroids. In addition, kaempferol markedly induced G2/M arrest and promoted apoptosis of HCC cells. Mechanically, kaempferol significantly reduced the protein and mRNA expression levels of ATM, CHEK2, CDC25C, CDK1, CCNB1, MPS1, KNL1, and Bub1. Additionally, the combination of kaempferol and the ATM inhibitor KU55933 had a more significant anti-HCC effect. The results of bioinformatics showed that ATM, CHEK2, CDC25C, CDK1, and KNL1 were highly expressed in patients with HCC and cancer tissues, indicating that these genes have certain value in the clinical diagnosis of HCC.
Collectively, our results revealed that kaempferol from A. officinarum inhibits the cell cycle by regulating the ATM/CHEK2/KNL1 pathway in HCC cells. In summary, our research presents an innovative supplementary strategy for HCC treatment.
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Polyester fabrics are attributed with various performances and are currently applied widely in textiles. This necessitates a quick and effective selection process to choose polyester fabrics to ...correspond with engineers' designs for industrial textiles. Therefore, the main focus of this paper is to present the comprehensive handle evaluation system for fabrics and yarns (CHES-FY), which has been specially developed to measure the basic handle of textile materials, including softness, stiffness, smoothness and tightness. Several kinds of polyester fabrics were chosen to undergo subjective evaluation and testing by the CHES-FY system, and were assigned into corresponding clusters by the K-means cluster method. The basic hand indexes of polyester fabric were featured. Comparisons between subjective judgments and the objective K-means cluster method were conducted. Experimental results show that a good correlation exists between subjective judgment and the objective cluster method, indicating that the four basic hand indexes measured by the CHES-FY system can be utilized to characterize the comprehensive hand of industrial polyester fabrics, and that the CHES-FY system can be used to discriminate categories of polyester fabrics.
Model updating in structural dynamics has attracted much attention in recent decades. And high computational cost is frequently encountered during model updating. Surrogate model has attracted ...considerable attention for saving computational cost in finite element model updating (FEMU). In this study, a model updating method using frequency response function (FRF) based on Kriging model is proposed. The optimal excitation point is selected by using modal participation criterion. Initial sample points are chosen via design of experiment (DOE), and Kriging model is built using the corresponding acceleration frequency response functions. Then, Kriging model is improved via new sample points using mean square error (MSE) criterion and is used to replace the finite element model to participate in optimization. Cuckoo algorithm is used to obtain the updating parameters, where the objective function with the minimum frequency response deviation is constructed. And the proposed method is applied to a plane truss model FEMU, and the results are compared with those by the second-order response surface model (RSM) and the radial basis function model (RBF). The analysis results showed that the proposed method has good accuracy and high computational efficiency; errors of updating parameters are less than 0.2%; damage identification is with high precision. After updating, the curves of real and imaginary parts of acceleration FRF are in good agreement with the real ones.
Aiming at the large tension of the solution obtained by sequential algorithm for optimal sensor placement problem, a novel relaxation sequential algorithm is proposed by introducing the idea of edge ...relaxation operation of Dijkstra’s algorithm into sequential algorithm. An initial solution set is generated by sequential algorithm, and improved by relaxation till the relaxation operation terminates. The proposed algorithm takes modal assurance criterion (MAC) matrix as the objective fitness function. A truss structure and a rigid-framed arch bridge are applied as examples to verify the effectiveness of the new algorithm for optimal sensor placement. The result indicates that the relaxation sequential algorithm requires fewer sensors and can reach better maximum off-diagonal element of MAC matrix in OSP problem.
Objective. Postoperative gastrointestinal dysfunction is a common and important complication of surgery. This study aimed to explore the key pharmacological mechanisms of Tongfu decoction in treating ...postoperative ileus (POI). Methods. The active ingredients of Tongfu decoction and their targets were screened using the TCMSP database and STITCH and SwissTargetPrediction databases, respectively. The GeneCards and DisGeNET databases were used to obtain POI dysfunction-related therapeutic targets. After screening, a drug-active-ingredient-therapeutic target network was constructed and the key target functional enrichment analysis was carried out. The Sprague–Dawley rats with POI were used for in vivo experimental verification. The serum levels of IL-1β, IL-6, IL-10, IFN-γ, and MCP-1 were measured after surgery using enzyme-linked immunosorbent assay. The Western blot analysis was used to determine the expression of key proteins of the PI3K-Akt signaling pathway in colon tissues. Results. An interaction network was constructed containing 7 Chinese medicine components, 36 compounds, and 85 target proteins. The functional enrichment analysis showed that the target proteins mainly acted on the POI through the PI3K-Akt signaling pathway. In in vivo experiments, Tongfu decoction had a promoting effect on the serum level of IL-10, an inhibitory effect on the serum levels of IL-1β and CCL2, and an inhibitory effect on the local expression of PI3K, pAkt, and mTOR in colon tissue. In addition, the Tongfu decoction increased the intestinal ink advancing rate. Conclusion. Nonoral Tongfu decoction can also be used to treat POI; its mechanism is affected by IL-10 and IL-1β.The inhibition of the PI3K-Akt signaling pathway affected the treatment with Tongfu decoction by inducing an immune-inflammatory storm in POI.
The arbitrary selection of phosphate fertilizer source and their low use efficiency in tobacco production makes it necessary to select rational phosphate fertilizer in purple soil, which is ...characterized with low soil phosphorus (P) availability. No phosphate fertilizer (control), single superphosphate (SSP), calcium–magnesium phosphate (CMP), ammonium polyphosphate (APP), monoammonium phosphate (MAP), and diammonium phosphate (DAP) were used to study the effects of different phosphate fertilizer source on inorganic P transformation, tobacco growth, and P utilization. During the 90-day incubation experiment, there were obvious differences in the dynamics of soil inorganic P fractions among the five P applied treatments, and the soil Olsen-P contents of all P applied treatments showed an increasing trend while soil pH showed the opposite trend. In the greenhouse pot experiment, the five P applied treatments increased the agronomic traits and P use efficiency (PUE) of tobacco compared to the control, and CMP and DAP had the larger increase of tobacco weight and PUE. Also, the soil Olsen-P, pH, phosphatase activities, and inorganic P fractions obviously differed among the five P applied treatments, the CMP had the highest alkaline phosphatase (ALP) activity and NH
4
Ac-extractable P (Ca
8
-P) contents, and DAP had the second-highest acid phosphatase (ACP) and neutral phosphatase (NP) activities and highest NaHCO
3
-extractable P (Ca
2
-P) content. In addition, the differences in the contents and proportions of inorganic P fractions between the indoor incubation soil and the tobacco-growing soil indicated that Ca
8
-P was an important source of P for tobacco growth. In all, the CMP and DAP are more suitable phosphate fertilizer source in purple soil, as they better promoted tobacco growth and development and P utilization.
Chlorogenic acid is an important polyphenolic compound in plants, which plays an important physiological role in plant growth and development, disease and pest resistance, cold resistance and other ...stresses. It has a variety of pharmacological functions, including anti-inflammatory, antibacterial, antiviral, anticancer, anti-ultraviolet light and radiation, immunoregulation, hypolipidemic, hypoglycemic, etc. Chlorogenic acid has great potential especially in functional foods, nutritional supplements, raw food materials, chemical industry and drug development. Tomato, eggplant, pepper, potato and tobacco are important cash crops in the world. They are all important types of Solanaceae plants, and also important sources of chlorogenic acid. The content of chlorogenic acid in fruits or vegetative organs of Solanaceae crops varies greatly, and their chlorogenic acid anabolism is a complex biological process determined by genetic and environmental factors. Therefore, the research on biosynthesis and molecular mechanisms of regulation of chlorogenic acid in Solanaceae crops has important theoretical significance and application value for crop genetic improvement. The article outlines the content of chlorogenic acid in the above five Solanaceae crops, the biosynthetic pathways and transcriptional regulation molecular mechanisms of chlorogenic acid, and the effects of genotype differences, environmental factors, cultivation measures on chlorogenic acid content in Solanaceae crops, which is expected to provide theoretical bases for the development and utilization of chlorogenic acid in Solanaceae crops, the creation of germplasm resources with high chlorogenic acid and the breeding of new varieties of Solanaceae crops.
【Objective】The study aims to comprehensively evaluate the ecological adaptability of different cigar varieties and screen cigar varieties suitable for planting in Zhanjiang tobacco areas of Guangdong ...Province.【Method】Field experiments were carried out on 8 newly introduced cigar varieties to analyze and compare their differences in field growth periods, agronomic traits, natural disease rates, conventional chemical components and sensory quality evaluation. Finally, comprehensive index method was used to evaluate their comprehensive traits.【Result】All the eight cigar varieties had a long field growth period, with an average of 141.36 days. The field growth periods of different cigar varieties varied greatly, with a maximum difference of 22 days (133 days for 'Haiyan 108' and 'Haiyan 201', 155 days for 'Yunxue 8'). 'Yunxue 8' and 'Haiyan 200' performed best at agronomic traits, their plant height, maximum leaf width, internodal length and effective leaf number were better than the average of the tested varieties, indicating that these two varieties had strong field growth potential. The incidence of tobacco mosaic virus of 'Haiyan 200', 'Haiyan 101' and 'Yunxue 2' was low, ranging from 5.09% to 6.85%, and no weather fleck occurred, indicating that the disease resistance of the three varieties was strong. 'Haiyan 200' and 'Haiyan 101' had better coordination of conventional chemical components, and their comprehensive scores were higher than the average level, however, the potassium chloride ratio of the two varieties was lower than the suitable range of high-quality tobacco leaves (4-10). In sensory quality evaluation, 'Haiyan 101' scored the highest (46.07), which was characterized by slightly sufficient aroma, clean aftertaste, strong sweetness and good burning ability. The results of comprehensive index calculation showed that the scores of eight cigar varieties ranked as follows: 'Haiyan 200' > 'Haiyan 101' > 'Yunxue 8' > 'Yunxue 2' > 'Haiyan 108' > 'Haiyan 201' > 'Yunxue 3' > 'Havana'.【Conclusion】'Haiyan 200' and 'Haiyan 101' have good comprehensive properties and were suitable for planting in Zhanjiang tobacco areas. It is suggested to further improve the quality of tobacco leaves by optimizing the fertilization scheme as well as drying and fermentation technology.
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