Oxidative stress is central to the pathology of several neurodegenerative diseases, including multiple sclerosis, and therapeutics designed to enhance antioxidant potential could have clinical value. ...The objective of this study was to characterize the potential direct neuroprotective effects of dimethyl fumarate (DMF) and its primary metabolite monomethyl fumarate (MMF) on cellular resistance to oxidative damage in primary cultures of central nervous system (CNS) cells and further explore the dependence and function of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway in this process. Treatment of animals or primary cultures of CNS cells with DMF or MMF resulted in increased nuclear levels of active Nrf2, with subsequent up-regulation of canonical antioxidant target genes. DMF-dependent up-regulation of antioxidant genes in vivo was lost in mice lacking Nrf2 Nrf2(-/-). DMF or MMF treatment increased cellular redox potential, glutathione, ATP levels, and mitochondrial membrane potential in a concentration-dependent manner. Treating astrocytes or neurons with DMF or MMF also significantly improved cell viability after toxic oxidative challenge in a concentration-dependent manner. This effect on viability was lost in cells that had eliminated or reduced Nrf2. These data suggest that DMF and MMF are cytoprotective for neurons and astrocytes against oxidative stress-induced cellular injury and loss, potentially via up-regulation of an Nrf2-dependent antioxidant response. These data also suggest DMF and MMF may function through improving mitochondrial function. The clinical utility of DMF in multiple sclerosis is being explored through phase III trials with BG-12, which is an oral therapeutic containing DMF as the active ingredient.
ABSTRACT Familial Mediterranean fever (FMF) is a hereditary disorder that presents with recurrent fever, rash, and polyserosal inflammation. The nonspecific symptoms of FMF allow it to mimic a large ...variety of diseases including metastatic Crohn's disease (MCD). MCD is a rare extraintestinal manifestation of Crohn's disease characterized by the presence of cutaneous noncaseating granulomas that are noncontiguous within the gastrointestinal tract. We describe a patient who had a delay in diagnosis of FMF as her clinical presentation mimicked MCD.
Endophytic bacteria present ubiquitously in all plant parts. Their community structure may vary depending on plant tissue and growth condition. This work mainly focused on exploring the diversity of ...culturable nitrogen-fixing endophytic bacteria in above-ground plant parts of wheat by harvesting it during various growth points (Seed stage, 1st, 2nd, and 3rd month old plants, respectively). Distinct endophytic bacterial colonies were selected on Jensen’s agar plate. Based on the 16S rRNA sequencing, 43 putative nitrogen-fixing endophytic bacteria were identified. Most of the isolates were found unique to the plant growth phase except for
Pseudomonas
sp.,
Bacillus
sp.,
Paenibacillus
sp.,
Microbacterium
sp.,
Exiguobacterium
sp. Further, endophytic bacteria were scrutinized for their plant growth promoting traits. They were found positive for IAA production (100%), P-solubilization (21%), Zn-solubilization (63%), ammonia production (93%), and
nifH
gene (33%). Extracellular enzyme production was found positive for cellulase (98%), pectinase (98%), and protease (100%). Their endophytic colonization ability was assessed using reactive oxygen species (ROS) induction assay, upon their entry inside the host plant.
COVID-19 is real a worldwide terrific problem. This paper focuses on the different aspects of data analytics and visualization by using various datasets supported by authorized sources. It also ...discusses the practical aspects using open source tools and python library support. Here chapter focuses on comparative analysis also. It also visualize analytical aspects by different aspects such as country wise, date wise and so on. In this paper, the COVID infected cases and its reaction on people will be discussed. This case study will predict the COVID-19 infected cases and death ratio with symptoms in future. This paper focus on data visualization, data analytics and comparative study based on practical aspects. Machine Learning plays a vital role to predict the cases by providing learning instances.
We study how double-stranded DNA translocates through graphene nanogaps. Nanogaps are fabricated with a novel capillary-force induced graphene nanogap formation technique. DNA translocation ...signatures for nanogaps are qualitatively different from those obtained with circular nanopores, owing to the distinct shape of the gaps discussed here. Translocation time and conductance values vary by ∼ 100%, which we suggest are caused by local gap width variations. We also observe exponentially relaxing current traces. We suggest that slow relaxation of the graphene membrane following DNA translocation may be responsible. We conclude that DNA-graphene interactions are important, and need to be considered for graphene-nanogap based devices. This work further opens up new avenues for direct read of single molecule activitities, and possibly sequencing.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
In the present study, blue light absorbing pigment protein phycoerythrin (PE) is purified up to molecular grade purity from marine Halomicronema sp. R31DM. The purification method is based on the use ...of non-ionic detergent Triton-X 100 in ammonium sulphate precipitation. The purified PE is characterized for its antioxidant activity in vitro and in vivo. PE is noted to show substantial in vitro antioxidant activity probed by various biochemical assays. The PE moderated rise in the intracellular-ROS (reactive oxygen species) in wild type Caenorhabditis elegans upon heat and oxidative stress. Further, the antioxidant asset of PE is noted an expedient in averting the ROS associated abnormalities, i.e. impaired physiological behaviour (health span) and aging in C. elegans. The structural attributes of PE contributing to its antioxidant virtue are analysed; the presence of ample residues having antioxidant activity and chromophore-PEB in PE are identified as a source of its antioxidant activity. Furthermore, the stability of PE is assessed under three physico-chemical stresses, temperature, pH and oxidative stress.
•The marine cyanobacterium is isolated and identified as Halomicronema sp. R31DM.•Phycoerythrin (PE) of molecular grade purity is obtained.•In vitro and in vivo antioxidant activity of Halomicronema PE is determined.•The antioxidant virtue of PE is studied by in-silico structure analysis.
Conduit arterial disease in chronic kidney disease (CKD) is an important cause of cardiac complications. Cardiac function in CKD has not been studied in the absence of arterial disease. In an Alport ...syndrome model bred not to have conduit arterial disease, mice at 225 days of life (dol) had CKD equivalent to humans with CKD stage 4-5. Parathyroid hormone (PTH) and FGF23 levels were one log order elevated, circulating sclerostin was elevated, and renal activin A was strongly induced. Aortic Ca levels were not increased, and vascular smooth muscle cell (VSMC) transdifferentiation was absent. The CKD mice were not hypertensive, and cardiac hypertrophy was absent. Freshly excised cardiac tissue respirometry (Oroboros) showed that ADP-stimulated O
flux was diminished from 52 to 22 pmol/mg (
= 0.022). RNA-Seq of cardiac tissue from CKD mice revealed significantly decreased levels of cardiac mitochondrial oxidative phosphorylation genes. To examine the effect of activin A signaling, some Alport mice were treated with a monoclonal Ab to activin A or an isotype-matched IgG beginning at 75 days of life until euthanasia. Treatment with the activin A antibody (Ab) did not affect cardiac oxidative phosphorylation. However, the activin A antibody was active in the skeleton, disrupting the effect of CKD to stimulate osteoclast number, eroded surfaces, and the stimulation of osteoclast-driven remodeling. The data reported here show that cardiac mitochondrial respiration is impaired in CKD in the absence of conduit arterial disease. This is the first report of the direct effect of CKD on cardiac respiration.
Heart disease is an important morbidity of chronic kidney disease (CKD). Hypertension, vascular stiffness, and vascular calcification all contribute to cardiac pathophysiology. However, cardiac function in CKD devoid of vascular disease has not been studied. Here, in an animal model of human CKD without conduit arterial disease, we analyze cardiac respiration and discover that CKD directly impairs cardiac mitochondrial function by decreasing oxidative phosphorylation. Protection of cardiac oxidative phosphorylation may be a therapeutic target in CKD.
Anthocyanins are a group of water‐soluble polyphenolic pigments found primarily in flowers, vegetables, and fruits. These pigments play critical roles in plant and human health. Spectrophotometric ...methods are a simple and inexpensive way to quantify anthocyanins in plant tissues. Two main spectrophotometric methods have been developed, organic solvent‐based, and pH differential methods. Both of these methods are subject to interference from light‐absorbing impurities and need to be optimized for different matrixes of different plant materials. Eight methods have been tested in this experiment to quantify anthocyanins in strawberry fruits. Six organic solvent‐based methods tested methanol, chloroform‐methanol, and MgO in different ratios. The other two methods were pH differential method and a combination of organic solvent‐based and pH differential method. Two methods used organic solvents (methanol and chloroform‐methanol) were the best in extracting anthocyanin from strawberry fruits. Adding MgO increased the pH of the extract and was less efficient in anthocyanin extraction. All other methods had lower anthocyanin yield compared with methanol and chloroform‐methanol methods and are not recommended for strawberry fruit anthocyanin extraction.
Two organic solvents, methanol and chloroform‐methanol, were the best in extracting anthocyanin from strawberry fruits. MgO decreased anthocyanin yield. All other methods had lower anthocyanin yield compared with methanol and chloroform‐methanol methods.
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