A tool, the Infection Risk Scan has been developed to measure the quality of infection control and antimicrobial use. This tool measures various patient-, ward- and care-related variables in a ...standardized way. We describe the implementation of this tool in nine hospitals in the Dutch/Belgian border area and the obtained results.
The IRIS consists of a set of objective and reproducible measurements: patient comorbidities, (appropriate) use of indwelling medical devices, (appropriate) use of antimicrobial therapy, rectal carriage of Extended-spectrum beta-lactamase producing Enterobacterales and their clonal relatedness, environmental contamination, hand hygiene performance, personal hygiene of health care workers and presence of infection prevention preconditions. The Infection Risk Scan was implemented by an expert team. In each setting, local infection control practitioners were trained to achieve a standardized implementation of the tool and an unambiguous assessment of data.
The IRIS was implemented in 34 wards in six Dutch and three Belgian hospitals. The tool provided ward specific results and revealed differences between wards and countries. There were significant differences in the prevalence of ESBL-E carriage between countries (Belgium: 15% versus The Netherlands: 9.6%), environmental contamination (median adenosine triphosphate (ATP) level Belgium: 431 versus median ATP level The Netherlands: 793) and calculated hand hygiene actions based on alcohol based handrub consumption (Belgium: 12.5/day versus The Netherlands: 6.3/day) were found.
The Infection risk Scan was successfully implemented in multiple hospitals in a large cross-border project and provided data that made the quality of infection control and antimicrobial use more transparent. The observed differences provide potential targets for improvement of the quality of care.
The objective of this study was to determine the level of environmental contamination in hospitals in the Dutch/Belgian border area, using ATP measurements.
A cross-sectional observational survey.
...Standardized ATP measurements were conducted in 9 hospitals on 32 hospital wards. Thirty pre-defined surfaces per hospital ward were measured with the 3 M Clean Trace NG luminometer. Results are displayed in relative light units (RLU). RLU > 1000 was considered as "not clean." Differences in RLU values were compared between countries, hospitals, fomite groups and medical specialties.
A total of 960 ATP measurements were performed, ranging from 60 up to 120 per hospital. The median RLU-value was 568 (range: 3-277,586) and 37.7% of the measurements were rated as not clean (RLU > 1000). There were significant differences between countries, hospitals and fomite groups.
ATP measurements can be used as a more objective approach to determine the level of environmental contamination in hospitals. Significant differences in ATP levels were found between hospitals and between countries. Also, substantial differences were found between different fomite groups. These findings offer potential targets for improvement of cleanliness in healthcare facilities.
Background A tool, the Infection Risk Scan has been developed to measure the quality of infection control and antimicrobial use. This tool measures various patient-, ward- and care-related variables ...in a standardized way. We describe the implementation of this tool in nine hospitals in the Dutch/Belgian border area and the obtained results. Methods The IRIS consists of a set of objective and reproducible measurements: patient comorbidities, (appropriate) use of indwelling medical devices, (appropriate) use of antimicrobial therapy, rectal carriage of Extended-spectrum beta-lactamase producing Enterobacterales and their clonal relatedness, environmental contamination, hand hygiene performance, personal hygiene of health care workers and presence of infection prevention preconditions. The Infection Risk Scan was implemented by an expert team. In each setting, local infection control practitioners were trained to achieve a standardized implementation of the tool and an unambiguous assessment of data. Results The IRIS was implemented in 34 wards in six Dutch and three Belgian hospitals. The tool provided ward specific results and revealed differences between wards and countries. There were significant differences in the prevalence of ESBL-E carriage between countries (Belgium: 15% versus The Netherlands: 9.6%), environmental contamination (median adenosine triphosphate (ATP) level Belgium: 431 versus median ATP level The Netherlands: 793) and calculated hand hygiene actions based on alcohol based handrub consumption (Belgium: 12.5/day versus The Netherlands: 6.3/day) were found. Conclusion The Infection risk Scan was successfully implemented in multiple hospitals in a large cross-border project and provided data that made the quality of infection control and antimicrobial use more transparent. The observed differences provide potential targets for improvement of the quality of care. Keywords: Infection prevention, Guidelines, Benchmarking, Surveillance
Glucose oxidase is one of the most conspicuous commercial enzymes due to its many different applications in diverse industries such as food, chemical, energy and textile. Among these applications, ...the most remarkable is the manufacture of glucose biosensors and in particular sensor strips used to measure glucose levels in serum. The generation of ameliorated versions of glucose oxidase is therefore a significant biotechnological objective. We have used a strategy that combined random and rational approaches to isolate uncharacterized mutations of Aspergillus niger glucose oxidase with improved properties. As a result, we have identified two changes that increase significantly the enzyme's thermal stability. One (T554M) generates a sulfur-pi interaction and the other (Q90R/Y509E) introduces a new salt bridge near the interphase of the dimeric protein structure. An additional double substitution (Q124R/L569E) has no significant effect on stability but causes a twofold increase of the enzyme's specific activity. Our results disclose structural motifs of the protein which are critical for its stability. The combination of mutations in the Q90R/Y509E/T554M triple mutant yielded a version of A. niger glucose oxidase with higher stability than those previously described.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Protein kinase C (PKC) plays a key role in embryonic stem cell (ESC) proliferation, self-renewal, and differentiation. However, the function of specific PKC isoenzymes have yet to be determined. Of ...the PKCs expressed in undifferentiated ESCs, βIPKC was the only isoenzyme abundantly expressed in the nuclei. To investigate the role of βΙPKC in these cells, we employed a phosphoproteomics strategy and used two classical (cPKC) peptide modulators and one βIPKC-specific inhibitor peptide. We identified 13 nuclear proteins that are direct or indirect βΙPKC substrates in undifferentiated ESCs. These proteins are known to be involved in regulating transcription, splicing, and chromatin remodeling during proliferation and differentiation. Inhibiting βΙPKC had no effect on DNA synthesis in undifferentiated ESCs. However, upon differentiation, many cells seized to express βΙPKC and βΙPKC was frequently found in the cytoplasm. Taken together, our results suggest that βIPKC takes part in the processes that maintain ESCs in their undifferentiated state.
Abstract 3037
In 2009, the Center for International Blood and Marrow Transplant Research (CIBMTR) published an updated, unified international set of post HCT vaccine guidelines which for the first ...time recommended vaccinating all patients with the protein conjugated pneumococcal vaccine (PCV7), rather than the pure polysaccharide vaccine PPV23, which is poorly immunogenic in this patient population. Due to the licensing of PCV13 in 2010 and cessation of PCV7 production, the guidelines were updated in 2010 to substitute PCV13 for PCV7. To date, there are limited studies evaluating PCV13 in HCT recipients. Since May of 2010, 59 patients transplanted at this center received three doses of PCV13 administered 1–3 months apart following an HLA-matched sibling (n=17) or alternative donor (n=42) HCT. The median patient age (range) at HCT was 39 (range: 3–68) years. The majority of patients were transplanted for acute leukemia (63%) or myelodysplastic syndrome (15%). Thirty-three percent of patients received an unmodified graft. Due to the variable kinetics of immune reconstitution post HCT in recipients of alternative donor HCTs compared to HLA matched siblings, patients were immunized upon acquisition of minimal milestones of immune competence (CD4 cell count >200 cells/ul and IgG level >500 mg/dl at least 6 weeks off gammaglobulin), rather than at fixed times post HCT. The median time to vaccination was 452 (range: 143–1187) days post HCT. Time to vaccination was significantly shorter in patients <21 versus >21 years of age (median: 318 vs 498 days, p=0.006) and significantly longer in patients who received post HCT rituximab versus those who did not (median: 610 versus 405 days, p=<0.001). Response was defined as seroconversion or 3 fold rise in titer against serotypes 1, 3, 4, 14, 19, 23, 26, 51, and 56. There were no serious adverse events attributable to the vaccine. Prior to immunization, 77% of patients were susceptible to >7 measured serotypes. Overall response was 73%, including 87% of patients <21 years of age at HCT and 67% of adults. Poorer responses were observed in recipients of post HCT rituximab despite a median CD19 cell count of 342 /ul at the time of vaccination. Response correlated with higher levels of circulating CD4+CD45RA+ T cells/ul (p=0.03) and CD27+, IgM- (memory isotype switched) B cells/ul, (p=0.03). This study suggests that PCV13 is very immunogenic when given upon acquisition of pre-set immune milestones. Results of an ongoing international trial in which PCV13 is initiated 3–6 months post HCT will determine its immunogenicity when given early post HCT. Prospective trials designed to identify biological markers predictive of an optimal and durable PCV13 response are needed.
Small:Pfizer, Inc: Equity Ownership, family member employed by Pfizer, Inc. Off Label Use: Use of 13cPnC in transplant patients. Perales:Pfizer, Inc: Equity Ownership.
Desde el lanzamiento del primer dispositivo tecnológico a mediados del siglo XX el mundo no imaginó el gran impacto que se alcanzaría con el ingreso a la nueva era digital.;
La forma de vivir, pensar ...y actuar de las personas ha sufrido drásticos cambios a nivel social, cultural y económico, debido a que las empresas han sabido sacarle provecho al avance tecnológico y se esfuerzan cada día más por conectar al mundo en una sola comunidad, dando como resultado que en la actualidad personas de toda edad, desde niños a adultos mayores, se encuentren interactuando a diario con dispositivos digitales.;
Ante ello, este plan de negocios se enfoca en una rama de investigación en particular: los niños entre las edades de 6 a 12 años de edad ubicados en Lima Metropolitana; y demuestra que existe una problemática latente que involucra el aprendizaje de aquellos niños que se encuentran expuestos a los medios tecnológicos y que eventualmente prefieren estar conectados a internet, dejando de lado el interés hacia sus actividades académicas habituales.;
ONClass es una plataforma web creada para dar solución a la preocupación que mantengan aquellos padres de familia con hijos menores en edad escolar, que pertenezcan a los NSE B y C, y que vean afectado el rendimiento escolar de sus hijos a causa del uso excesivo de la tecnología, brindando un servicio de aprendizaje y reforzamiento diferente e innovador que combina cursos escolares, tecnología y juegos para alcanzar un mejor aprovechamiento académico y la retención constante del conocimiento.
Since the launch of the first technological device in the mid-twentieth century the world did not imagine the great impact that would be achieved with the entry to the new digital era.;
The way of living, thinking and acting of people has undergone drastic social, cultural and economic changes, because companies have known how to take advantage of technological progress and strive every day to connect the world in one community, as a result, people of all ages, from children to older adults, are interacting daily with digital devices.;
In view of this, this business plan focuses on a particular research branch: children between the ages of 6 to 12 years of age located in Metropolitan Lima; and it shows that there is a latent problem that involves the learning of those children who are exposed to technologic media and who eventually prefer to be connected to the internet, leaving aside their interests in their usual academic activities.;
ONClass is a web platform created to solve the concern of parents with children under school age, who belong to the NSE B and C, and who are affected the school performance of their children because of excessive use of technology, providing a different and innovative learning and reinforcement service that combines school courses, technology and games to achieve better academic achievement and constant retention of knowledge.
Trabajo de investigación
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