Water quality of the inner Puno Bay was evaluated; we established 12 stations, and were assessed monthly from December 2010 to April 2011. Physicochemical water parameters were determined with EPA ...and APHA standard methods. Nutrients were determined spectrophotometrically. The parameters of temperature, dissolved oxygen, pH, phosphates, nitrates and nitrites of water show that the outlet of the stabilization lagoon of Puno City (Espinar Island) is a critical area of contamination at the inner Puno Bay. Transparency values were low. The electrical conductivity of water showed high values. Alkalinity values were high (75 - 150 mg/L) and very high (> 150 mg/L), indicating a high content of carbonates and bicarbonates. Water hardness were high (121-180 mg/L) and very high (> 180 mg/L). High levels of fecal coliform in waters near the island Espinar would be the result of wastewater discharges from the Puno city, without proper treatment.
Water quality of the inner Puno Bay was evaluated; we established 12 stations, and were assessed monthly from December 2010 to April 2011. Physicochemical water parameters were determined with EPA ...and APHA standard methods. Nutrients were determined spectrophotometrically. The parameters of temperature, dissolved oxygen, pH, phosphates, nitrates and nitrites of water show that the outlet of the stabilization lagoon of Puno City (Espinar Island) is a critical area of contamination at the inner Puno Bay. Transparency values were low. The electrical conductivity of water showed high values. Alkalinity values were high (75 - 150 mg/L) and very high (> 150 mg/L), indicating a high content of carbonates and bicarbonates. Water hardness were high (121- 180 mg/L) and very high (> 180 mg/L). High levels of fecal coliform in waters near the island Espinar would be the result of wastewater discharges from the Puno city, without proper treatment.
EcoCyc (http://EcoCyc.org) is a comprehensive model organism database for Escherichia coli K-12 MG1655. From the scientific literature, EcoCyc captures the functions of individual E. coli gene ...products; their regulation at the transcriptional, post-transcriptional and protein level; and their organization into operons, complexes and pathways. EcoCyc users can search and browse the information in multiple ways. Recent improvements to the EcoCyc Web interface include combined gene/protein pages and a Regulation Summary Diagram displaying a graphical overview of all known regulatory inputs to gene expression and protein activity. The graphical representation of signal transduction pathways has been updated, and the cellular and regulatory overviews were enhanced with new functionality. A specialized undergraduate teaching resource using EcoCyc is being developed.
EcoCyc (http://EcoCyc.org) is a model organism database built on the genome sequence of Escherichia coli K-12 MG1655. Expert manual curation of the functions of individual E. coli gene products in ...EcoCyc has been based on information found in the experimental literature for E. coli K-12-derived strains. Updates to EcoCyc content continue to improve the comprehensive picture of E. coli biology. The utility of EcoCyc is enhanced by new tools available on the EcoCyc web site, and the development of EcoCyc as a teaching tool is increasing the impact of the knowledge collected in EcoCyc.
EcoCyc (http://EcoCyc.org) provides a comprehensive encyclopedia of Escherichia coli biology. EcoCyc integrates information about the genome, genes and gene products; the metabolic network; and the ...regulatory network of E. coli. Recent EcoCyc developments include a new initiative to represent and curate all types of E. coli regulatory processes such as attenuation and regulation by small RNAs. EcoCyc has started to curate Gene Ontology (GO) terms for E. coli and has made a dataset of E. coli GO terms available through the GO Web site. The curation and visualization of electron transfer processes has been significantly improved. Other software and Web site enhancements include the addition of tracks to the EcoCyc genome browser, in particular a type of track designed for the display of ChIP-chip datasets, and the development of a comparative genome browser. A new Genome Omics Viewer enables users to paint omics datasets onto the full E. coli genome for analysis. A new advanced query page guides users in interactively constructing complex database queries against EcoCyc. A Macintosh version of EcoCyc is now available. A series of Webinars is available to instruct users in the use of EcoCyc.
This work assessed the effectiveness of several methods on degradation of microcystin-LR (MC-LR) by different Advanced Oxidation Processes, like solar photo-Fenton, UV-A/photo-Fenton and UV-C/H2O2. ...UV-C/H2O2 and UV-A/photo-Fenton processes were carried out in a bench scale photochemical apparatus and the solar photo-Fenton treatment was performed in a CPC photoreactor. MC-LR degradation was monitored by LC-ESI-MS/MS and kinetic parameters were calculated for all systems evaluated. The results demonstrated that UV-C/H2O2 was the most efficient method, showing a reduction of over 90% of initial MC-LR after 5 min of reaction. Solar and photo-Fenton/UVA had a rate decrease of 88 and 76% after the same time, respectively. The kinetic study indicated that the solar photo-Fenton and artificial radiation (UV-A) processes were very similar in their efficiency. The use of sunlight instead of artificial UV radiation significantly reduced the cost of photocatalytic treatment systems; it is also an environmentally friendly method, since it utilizes renewable energy.
Background and Aims
Hepatic ischemia–reperfusion injury (IRI) is the leading cause of early posttransplantation organ failure as mitochondrial respiration and ATP production are affected. A shortage ...of donors has extended liver donor criteria, including aged or steatotic livers, which are more susceptible to IRI. Given the lack of an effective treatment and the extensive transplantation waitlist, we aimed at characterizing the effects of an accelerated mitochondrial activity by silencing methylation‐controlled J protein (MCJ) in three preclinical models of IRI and liver regeneration, focusing on metabolically compromised animal models.
Approach and Results
Wild‐type (WT), MCJ knockout (KO), and Mcj silenced WT mice were subjected to 70% partial hepatectomy (Phx), prolonged IRI, and 70% Phx with IRI. Old and young mice with metabolic syndrome were also subjected to these procedures. Expression of MCJ, an endogenous negative regulator of mitochondrial respiration, increases in preclinical models of Phx with or without vascular occlusion and in donor livers. Mice lacking MCJ initiate liver regeneration 12 h faster than WT and show reduced ischemic injury and increased survival. MCJ knockdown enables a mitochondrial adaptation that restores the bioenergetic supply for enhanced regeneration and prevents cell death after IRI. Mechanistically, increased ATP secretion facilitates the early activation of Kupffer cells and production of TNF, IL‐6, and heparin‐binding EGF, accelerating the priming phase and the progression through G1/S transition during liver regeneration. Therapeutic silencing of MCJ in 15‐month‐old mice and in mice fed a high‐fat/high‐fructose diet for 12 weeks improves mitochondrial respiration, reduces steatosis, and overcomes regenerative limitations.
Conclusions
Boosting mitochondrial activity by silencing MCJ could pave the way for a protective approach after major liver resection or IRI, especially in metabolically compromised, IRI‐susceptible organs.
El estudio tuvo como ob¡etivo describir la propensión de estudiantes universitarios peruanos a las dificultades de aprendizaje, problemas del desarrollo y otras dificultades relacionadas al quehacer ...universitario. Se realizó una investigación descriptiva con dos muestras conformas por 119 estudiantes pertenecientes al semestre 2020-I y 169 del semestre 2020II. Se utilizó la escala DASS-21 para ansiedad, depresión y estrés; el cuestionario de Pittsburg de calidad de sueño, el cuestionario breve CBA y el cuestionario para identificar las dificultades de aprendizaje, sensoriales y motores. Los resultados señalan que los estudiantes presentaron mayor propensión a las dificultades relacionadas con la privación de sueño, dificultades con las matemáticas, ansiedad no patológica, dificultades de la escritura, ortografía y comprensión lectora y, por último, limitaciones visuales. Finalmente, se concluyó que existen dificultades de aprendizaje, privación de sueño, trastorno de adaptación con estados de ánimo deprimido y el permisible trastorno por consumo de alcohol en los estudiantes universitarios de la Amazonía peruana.
La investigación se orientó a establecer la asociación entre las características motivacionales y la actitud hacia la ecoeficiencia en los trabajadores de la universidad pública peruana. El estudio ...se realizó en base a la metodología cuantitativa, asimismo corresponde al tipo de estudio descriptivo correlacional con una muestra de 148 participantes. Los strumentos de de datos fueron el Cuestionario de Motivación Laboral y el Cuestionario de Actitud Ecoeficiente, compuestos en cada caso por 30 ítems de tipo Likert. Según los resultados, las características motivacionales tienden a favorecer la motivación de los trabajadores, la mayoría de ellos presentan una alta disposición hacia el uso eficiente de los recursos y mediante la prueba de Chi Cuadrado se obtuvo una significación asintótica (bilateral) inferior al nivel de significancia (p<0,05). Se concluyó que existe dependencia entre las variables características motivacionales con la actitud hacia la ecoeficiencia en los trabajadores universitarios. En ese sentido, las características motivacionales podrían ayudar a predecir la actitud hacia la ecoeficiencia.
PDF
