Gejala mosaik kuning dan keriting daun ditemukan pada pertanaman kacang panjang di Sleman, Yogyakarta. Begomovirus diketahui sebagai salah satu penyebab penyakit tersebut. Penelitian dilakukan untuk ...mengidentifikasi spesies Begomovirus dan DNA satelit yang berasosiasi dengan penyakit mosaik kuning kacang panjang. Ekstraksi DNA total dari tanaman bergejala dilanjutkan dengan amplifikasi fragmen DNA spesifik Begomovirus dan Betasatelit. Amplikon DNA berukuran ±1500 pb dan ±1300 pb berhasil diperoleh menggunakan berturut-turut primer universal Begomovirus dan primer spesifik Betasatelit. Analisis sekuen nukleotida mengonfirmasi identitas Begomovirus yang menginfeksi tanaman kacang panjang ialah Mungbean yellow mosaic India virus (MYMIV) dengan homologi 99% terhadap isolat MYMIV asal Indonesia. DNA satelit yang berasosiasi dengan MYMIV menunjukkan karakteristik Betasatelit, yaitu memiliki satellite common region (SCR) dengan struktur stem-loop dan sekuen TAATATTAC pada bagian loop, adenine rich region sebesar 54.96%, dan ORF (open reading frame) non-coding. Lebih lanjut, analisis rekombinasi menggunakan SimPlot mengindikasikan bahwa satelit non-coding MYMIV merupakan satelit rekombinan antara Betasatelit dan DNA-B Pepper yellow leaf curl Indonesia virus (PepYLCIV). Artikel ini merupakan laporan pertama asosiasi betasatelit DNA non-coding dengan MYMIV di Indonesia.
In fruiting bodies and mycelia of several fungi belonging to a Basidiomycetes group, among others, Ganoderma contains active polysaccharides and has potential as an antiviral substances. This study ...aimed to determine the effect of mycelium and fruiting body extract of Ganoderma sp. against Rehmannia mosaic virus (ReMV) infections on Chenopodium amaranticolor with variations of dilution and application times. The results showed that mycelium and fruiting body extract of Ganoderma sp. could inhibit ReMV infections. Antiviral extract which were applied 4 hours before, and at the same time with virus inoculation resulted in a higher viral inhibition rates than when applied 4 hours after virus inoculation. Fruiting body extract at 10-1 and 10-2 dilutions delayed symptom appearance caused by ReMV infection when applied 4 hours before virus inoculation. Mycelium extract at dilution of 10-2 and 10-3 applied 4 hours before virus inoculation resulted in the highest inhibition rate (100%) which was indicated as the number of local lesions on Chenopodium amaranticolor leaves. Meanwhile the fruiting body extract dilution of 10-1 resulted in highest inhibition (99.55%) when applied 4 hours before virus inoculation.
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