Objective
To provide updated guidelines for pharmacologic management of juvenile idiopathic arthritis (JIA), focusing on treatment of oligoarthritis, temporomandibular joint (TMJ) arthritis, and ...systemic JIA with and without macrophage activation syndrome. Recommendations regarding tapering and discontinuing treatment in inactive systemic JIA are also provided.
Methods
We developed clinically relevant Patient/Population, Intervention, Comparison, and Outcomes questions. After conducting a systematic literature review, the Grading of Recommendations Assessment, Development and Evaluation approach was used to rate the quality of evidence (high, moderate, low, or very low). A Voting Panel including clinicians and patients/caregivers achieved consensus on the direction (for or against) and strength (strong or conditional) of recommendations.
Results
Similar to those published in 2019, these JIA recommendations are based on clinical phenotypes of JIA, rather than a specific classification schema. This guideline provides recommendations for initial and subsequent treatment of JIA with oligoarthritis, TMJ arthritis, and systemic JIA as well as for tapering and discontinuing treatment in subjects with inactive systemic JIA. Other aspects of disease management, including factors that influence treatment choice and medication tapering, are discussed. Evidence for all recommendations was graded as low or very low in quality. For that reason, more than half of the recommendations are conditional.
Conclusion
This clinical practice guideline complements the 2019 American College of Rheumatology JIA and uveitis guidelines, which addressed polyarthritis, sacroiliitis, enthesitis, and uveitis. It serves as a tool to support clinicians, patients, and caregivers in decision‐making. The recommendations take into consideration the severity of both articular and nonarticular manifestations as well as patient quality of life. Although evidence is generally low quality and many recommendations are conditional, the inclusion of caregivers and patients in the decision‐making process strengthens the relevance and applicability of the guideline. It is important to remember that these are recommendations. Clinical decisions, as always, should be made by the treating clinician and patient/caregiver.
Deforested and converted tropical peat swamp forests are susceptible to fires and are a major source of greenhouse gas (GHG) emissions. However, information on the influence of land-use change (LUC) ...on the carbon dynamics in these disturbed peat forests is limited. This study aimed to quantify soil respiration (heterotrophic and autotrophic), net primary production (NPP), and net ecosystem production (NEP) in peat swamp forests, partially logged forests, early seral grasslands (deforested peat), and smallholder-oil palm estates (converted peat). Peat swamp forests (PSF) showed similar soil respiration with logged forests (LPSF) and oil palm (OP) estates (37.7 Mg CO2 ha−1 yr−1, 40.7 Mg CO2 ha−1 yr−1, and 38.7 Mg CO2 ha−1 yr−1, respectively), but higher than early seral (ES) grassland sites (30.7 Mg CO2 ha−1 yr−1). NPP of intact peat forests (13.2 Mg C ha−1 yr−1) was significantly greater than LPSF (11.1 Mg C ha−1 yr−1), ES (10.8 Mg C ha−1 yr−1), and OP (3.7 Mg C ha−1 yr−1). Peat swamp forests and seral grasslands were net carbon sinks (10.8 Mg CO2 ha−1 yr−1 and 9.1 CO2 ha−1 yr−1, respectively). In contrast, logged forests and oil palm estates were net carbon sources; they had negative mean Net Ecosystem Production (NEP) values (−0.1 Mg CO2 ha−1 yr−1 and −25.1 Mg CO2 ha−1 yr−1, respectively). The shift from carbon sinks to sources associated with land-use change was principally due to a decreased Net Primary Production (NPP) rather than increased soil respiration. Conservation of the remaining peat swamp forests and rehabilitation of deforested peatlands are crucial in GHG emission reduction programs.
Species management and conservation efforts are often based on range-wide trends, assuming dynamic equilibrium across space and time, even though fine-scale variability may be driving local dynamics. ...Oncorhynchus mykiss is a globally introduced, facultatively anadromous salmonid that is experiencing demographic shifts characterized by greater proportions of population remaining in freshwater. The degree of niche overlap between age classes of O. mykiss in freshwater environments may dictate how resources are partitioned within populations. We conducted a meta-analysis of age-specific O. mykiss habitat use to evaluate the degree of niche-partitioning between age classes and how age-specific habitat use relates to global in-stream and landscape level habitat variation. O. mykiss used deeper habitats as they grew towards maturity but did not partition habitat based on water velocity or substrate composition. As annual precipitation increased, O. mykiss used deeper and shallower habitats, and as summer air temperature increased, O. mykiss used shallower habitats. O. mykiss of native origin used deeper habitats than nonnative O. mykiss. However, a large proportion (42–99%) of variation in habitat use was associated with study or ecoregion, making climactic predictors unreliable for predictive species distribution or population dynamics models. Although the exact mechanisms driving geographic variability in O. mykiss habitat use are not fully understood, our results boost our understanding of how demographic shifts affect population resilience under climate change. Further research incorporating individual competitive behavior in predictive population models may elucidate the links between resource availability, demographic rates, and long-term O. mykiss population stability.
Studies on hydromineral balance in fishes frequently employ measurements of electrolytes following euthanasia. We tested the effects of fresh- or salt-water euthanasia baths of tricaine mesylate ...(MS-222) on plasma magnesium (Mg2+) and sodium (Na+) ions, cortisol and osmolality in fish exposed to saltwater challenges, and the ion and steroid hormone fluctuations over time following euthanasia in juvenile spring Chinook salmon (Oncorhynchus tshawytscha). Salinity of the euthanasia bath affected plasma Mg2+ and Na+ concentrations as well as osmolality, with higher concentrations in fish euthanized in saltwater. Time spent in the bath positively affected plasma Mg2+ and osmolality, negatively affected cortisol, and had no effect on Na+ concentrations. The difference of temporal trends in plasma Mg2+ and Na+ suggests that Mg2+ may be more sensitive to physiological changes and responds more rapidly than Na+. When electrolytes and cortisol are measured as endpoints after euthanasia, care needs to be taken relative to time after death and the salinity of the euthanasia bath.
Background: Pneumococcal disease remains a public health priority in adults. Previous studies have suggested that administration of pneumococcal polysaccharide vaccine or pneumococcal conjugate ...vaccine within three years following receipt of PPV23 was associated with increased reactogenicity and reduced antibody titers in comparison to longer intervals. Safety and immunogenicity of 15-valent pneumococcal conjugate vaccine (PCV15) was evaluated in adults ≥ 65 years of age with prior history of PPV23 vaccination (V114-007; NCT02573181).
Methods: A total of 250 adults who received PPV23 at least 1 year prior to study entry received a single dose of either PCV15 or PCV13 (125/arm) and were followed for safety for 14 days postvaccination. Serotype-specific Immunoglobulin G (IgG) geometric mean concentrations (GMCs) and opsonophagocytic activity (OPA) geometric mean titers (GMTs) were measured immediately prior and 30 days postvaccination.
Results: Safety profiles were comparable between PCV15 and PCV13 recipients. Following vaccination, serotype-specific antibody responses for the 13 shared serotypes were generally comparable between recipients of PCV15 and PCV13 for IgG GMCs, OPA GMTs, and geometric mean fold rises (GMFRs) and percentages of subjects with ≥ 4-fold-rise from baseline for both IgG and OPA. Recipients of PCV15 had numerically higher antibody responses than PCV13 for two serotypes unique to PCV15 (22F, 33F).
Conclusion: PCV15 was generally well tolerated and induced high levels of IgG and OPA antibodies to all 15 serotypes included in the vaccine when given as a single dose to adults ≥ 65 years of age previously vaccinated with PPV23.
Context
Amphibian conservation efforts commonly assume populations are tied to waterbodies that collectively function as a metapopulation. This assumption is rarely evaluated, and there is a need to ...understand the degree of connectivity among patches to appropriately define, manage, and conserve biological populations.
Objectives
Our objectives were to quantify local persistence, colonization, and recruitment (metademographic rates) in relation to habitat attributes, evaluate the influence of the spatial arrangement of patches on landscape-scale population dynamics, and estimate the scale at which metapopulation dynamics are occurring for Oregon spotted frog (
Rana pretiosa
).
Methods
We collected
R. pretiosa
detection/non-detection data and habitat information from 93 sites spread throughout the species’ extant range in Oregon, USA, 2010–2018. We developed a spatial multistate dynamic occupancy model to analyze these data.
Results
The proportion of sites occupied by
R. pretiosa
was relatively stable despite regular turnover in site occupancy. Connectivity was greatest when the distance between sites was within 4.49–7.70 km, and the results suggested that populations within 1 km are at the appropriate spatial scale for effective population management.
Rana pretiosa
metademographic rates were strongly tied to water availability, vegetation characteristics, and beaver dams.
Conclusions
Our analysis provides critical information to identify the appropriate spatial scale for effective population management, estimates the distance at which populations are connected, and quantifies the effects of hypothesized threats to species at a landscape scale. We believe this model will prove to be useful to inform conservation and management strategies for multiple species.
The present study evaluated the effects of nicotine concentration (0-10 mg/ml) and flavor (gummy bear vs unflavored) on the subjective experiences of vaporized nicotine in young adult low-dose ...nicotine (3 mg/ml) ECIG users.
Eight young adult ECIG users were recruited.
A single blinded crossover study was used. Participants were instructed to take ten 1.5 second puffs, each separated by 20 seconds. After self-administration, heart rate was recorded, and participants completed the Drug Effects, Direct Effects of Nicotine, and Direct Effects of ECIG questionnaires.
ECIG user's standard daily nicotine dose influenced the rewarding and aversive effects of nicotine as the 10 mg/ml dose was found to be aversive in this user group. The combination of flavor and nicotine increased the subjective effects of ECIGs.
Flavored e-liquids contribute to the reinforcing properties of nicotine by enhancing the subjective effects, which may lead to continued ECIG use.
Here, we provide evidence that the freshwater parasitic copepod, Salmincola californiensis, acts as a vector for Aeromonas salmonicida. While investigating the effects of S. californiensis on ...Chinoook salmon (Oncorhynchus tshawytscha), we tangentially observed that fish infected with the copepod developed furunculosis, caused by A. salmonicida. This occurred despite being reared in pathogen-free well water in a research facility with no prior history of spontaneous infection. We further investigated the possibility of S. californiensis to serve as a vector for the bacterium via detection of fluorescently labelled A. salmonicida inside the egg sacs from copepods in which the fish hosts were experimentally infected with GFP-A449 A. salmonicida. We then evaluated copepod egg sacs that were collected from adult Chinook salmon from a freshwater hatchery with A. salmonicida infections confirmed by either culture or PCR. The bacterium was cultured on tryptic soy agar plates from 75% of the egg sacs, and 61% were positive by PCR. These three separate experiments indicate an alternative tactic of transmission in addition to direct transmission of A. salmonicida in captivity. The copepod may play an important role in transmission of the bacterium when fish are more dispersed, such as in the wild.
Freshwater mussel populations are highly susceptible to environmental alterations because of their diminished numbers and primarily sessile behaviors; nonlethal biomonitoring programs are needed to ...evaluate the health of populations prior to mass mortality events. Our objectives were to determine (i) which biochemical parameters in freshwater mussel hemolymph could be consistently quantified, (ii) how hemolymph parameters and tissue glycogen respond to a thermal stress gradient (25, 30, and 35°C), and (iii) the effects of tissue and hemolymph extraction on long-term growth and survival of smallerand larger-bodied mussel species. Glucose exhibited elevated expression in both species with increasing water temperature. Two transaminase enzymes had elevated expression in the 30°C treatment. The effects of hemolymph extraction and tissue biopsies were evaluated with a large-bodied species, Elliptio crassidens, and a smaller species, Villosa vibex. Individuals were monitored for 820 to 945 days after one of four treatments: hemolymph extraction, tissue biopsy, tissue and hemolymph extraction, and control. Hemolymph extraction and tissue biopsy adversely affected survival of V. vibex, suggesting that these extraction methods may add some risk of reduced survival to smaller-bodied species. Survival of E. crassidens was not impaired by any of the treatments, supporting the use of these techniques in nonlethal biomonitoring programs for larger-bodied mussel species.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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