Argyrins are a group of anticancer and antibacterial octapeptide bioactive substances isolated from myxobacteria. In this study, we showed that the myxobacterium Archangium gephyra MEHO_001, isolated ...in Korea, produces argyrins A and B. MEHO_001 cells tend to aggregate when cultured in liquid media. Hence, a dispersion mutant, MEHO_002, was isolated from MEHO_001. The MEHO_002 strain produced approximately 3.5 times more argyrins than that produced by the wild-type strain MEHO_001. We determined the whole-genome sequence of A. gephyra MEHO_002 and identified a putative argyrin biosynthetic gene cluster comprising five genes, arg1-arg5, encoding non-ribosomal peptide synthases and tailoring enzymes. Inactivation of arg2 by plasmid insertion disrupted argyrin production. The amino acid sequences of the proteins encoded by arg2-arg5 of A. gephyra MEHO_002 were 90-98% similar to those encoded by the argyrin biosynthetic genes of Cystobacter sp. SBCb004, an argyrin-producing myxobacterium with identical domain organization.
Argyrins are a group of anticancer and antibacterial octapeptide bioactive substances isolated from myxobacteria.
In this study, we showed that the myxobacterium Archangium gephyra MEHO_001, isolated ...in Korea, produces argyrins A and B. MEHO_001 cells tend to aggregate when cultured in liquid media.
Hence, a dispersion mutant, MEHO_002, was isolated from MEHO_001. The MEHO_002 strain produced approximately 3.5 times more argyrins than that produced by the wild-type strain MEHO_001. We determined the whole-genome sequence of A. gephyra MEHO_002 and identified a putative argyrin biosynthetic gene cluster comprising five genes, arg1-arg5, encoding non-ribosomal peptide synthases and tailoring enzymes.
Inactivation of arg2 by plasmid insertion disrupted argyrin production. The amino acid sequences of the proteins encoded by arg2-arg5 of A. gephyra MEHO_002 were 90-98% similar to those encoded by the argyrin biosynthetic genes of Cystobacter sp. SBCb004, an argyrin-producing myxobacterium with identical domain organization. KCI Citation Count: 0
Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function of the eukayotic cytoskeleton. We developed a pair of PCR primers that specifically amplified ...tubulysin biosynthetic genes. Using these primers, eight out of the eighty-one strains of myxobacteria belonging to the Cystobacteraceae family that harbored putative tubulysin biosynthetic genes were screened through PCR analysis. The selected strains included two Archangium gephyra, two Stigmatella sp., two Vitiosangium cumulatum, and two unidentified myxobacteria. LC-MS analysis of the culture extracts from the selected strains revealed that A. gephyra KYC4066 produced putative tubulysin A and B.
Tubulysin은 다양한 암세포주에 대해 강한 항암활성을 보이는 점액세균 유래 이차대사 생리활성물질이다. 본 연구에서는 tubulysin을 생산하는 두 균주의 점액세균 Archangium gephyra MEHO_002와 MEHO_004의 유전체 분석을 통해 tubulysin 생합성 유전자들로 추정되는 유전자군을 발견하였으며, 플라스미드 삽입에 의한 ...유전자 불활성화를 통해 이들 유전자들이 tubulysin 생산과 직접 연관되어 있음을 확인하였다. A. gephyra MEHO_002와 MEHO_004 균주의 tubulysin 생합성 유전자군(tubA~tubF)은 DNA 염기서열이 서로 97% 동일하였으며, 암호화하는 단백질들의 아미노산 서열도 서로 97-100% 유사하였다. MEHO_002와 MEHO_004 균주의 tubulysin 생합성 유전자군은 tubulysin 생산 점액세균으로 알려진 Cystobacter sp. SBCb004의 tubulysin 생합성 유전자군과 DNA 염기서열이 86% 동일하였다. 유전자군의 구성은 tubZ 유전자가 존재하지 않는다는 점을 제외하고는 SBCb004의 tubulysin 생합성 유전자군 구성과 동일하였다. 각 유전자가 암호화하는 단백질의 아미노산 서열은 Cystobacter sp. SBCb004의 tubulysin 생합성 유전자가 암호화하는 단백질들과 88-97% 유사하였으며, 각 단백질들의 도메인 구성도 동일하였다.
Tubulysins are a group of bioactive secondary metabolites from myxobacteria exhibiting strong anticancer activity against various cancer cell lines. In this study, we describe the identification of putative tubulysin biosynthetic gene clusters (tubA~tubF) in the genome sequences of two tubulysin-producing myxobacterial strains, Archangium gephyra MEHO_002 and MEHO_004. The inactivation of the putative tubulysin biosynthetic genes resulted in a tubulysin-production defect. The DNA sequences of the A. gephyra MEHO_002 and MEHO_004 tubulysin biosynthetic genes were 97% identical, and the amino acid sequences of the encoded proteins shared a similarity of 97-100%. The nucleotide sequences of the tubulysin biosynthetic gene clusters in MEHO_002 and MEHO_004 were 86% identical to that in Cystobacter sp. SBCb004 known as a tubulysin-producing myxobacterium, and the organization of the clusters was identical except for the lack of a tubZ gene in the clusters in MEHO_002 and MEHO_004. The amino acid sequences of the proteins encoded by each gene were 88-97% similar to those encoded by SBCb004, and the domain compositions of the proteins were also identical.
Tubulysins are a group of bioactive secondary metabolites from myxobacteria exhibiting strong anticancer activity against various cancer cell lines. In this study, we describe the identification of ...putative tubulysin biosynthetic gene clusters (tubA~tubF) in the genome sequences of two tubulysin-producing myxobacterial strains, Archangium gephyra MEHO_002 and MEHO_004. The inactivation of the putative tubulysin biosynthetic genes resulted in a tubulysin-production defect. The DNA sequences of the A. gephyra MEHO_002 and MEHO_004 tubulysin biosynthetic genes were 97% identical, and the amino acid sequences of the encoded proteins shared a similarity of 97−100%. The nucleotide sequences of the tubulysin biosynthetic gene clusters in MEHO_002 and MEHO_004 were 86% identical to that in Cystobacter sp. SBCb004 known as a tubulysin-producing myxobacterium, and the organization of the clusters was identical except for the lack of a tubZ gene in the clusters in MEHO_002 and MEHO_004. The amino acid sequences of the proteins encoded by each gene were 88-97% similar to those encoded by SBCb004, and the domain compositions of the proteins were also identical. KCI Citation Count: 0
Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function of the eukayotic cytoskeleton. We developed a pair of PCR primers that specifically amplified ...tubulysin biosynthetic genes. Using these primers, eight out of the eighty-one strains of myxobacteria belonging to the Cystobacteraceae family that harbored putative tubulysin biosynthetic genes were screened through PCR analysis. The selected strains included two Archangium gephyra, two Stigmatella sp., two Vitiosangium cumulatum, and two unidentified myxobacteria. LC-MS analysis of the culture extracts from the selected strains revealed that A. gephyra KYC4066 produced putative tubulysin A and B. KCI Citation Count: 1
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