We study the equation of state of symmetric nuclear matter at zero temperature over a wide range of densities using two complementary theoretical approaches. At low densities, up to twice nuclear ...saturation density, we compute the energy per particle based on modern nucleon-nucleon and three-nucleon interactions derived within chiral effective field theory. For higher densities, we derive for the first time constraints in a Fierz-complete setting directly based on quantum chromodynamics using functional renormalization group techniques. We find remarkable consistency of the results obtained from both approaches as they come together in density and the natural emergence of a maximum in the speed of sound c S at supranuclear densities. The presence of this maximum appears tightly connected to the formation of a diquark gap. Notably, this maximum is observed to exceed the asymptotic value cS2 = 1 / 3 while its exact position in terms of the density cannot yet be determined conclusively.
The aim of this study was evaluated the effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages based on chitosan using the ELISA. The analysed protein ...was the tropomyosin. The results confirmed the presence of the tropomyosin (3.77 ± 0.79-5.75 ± 0.01 µg/g) in control samples. This study demonstrated that the bioactive substances in the form of grape (0.61 ± 0.34-0.43 ± 0.16 µg/g), blueberry (0.58 ± 0.32-0.39 ± 0.27 µg/g), and parsley extracts (2.09 ± 1.28-0.79 ± 0.40 µg/g) reduces immunoreactivity (p < 0.05) of the tropomyosin. The elder pollen had no significant effect (p > 0.05) on immunoreactivity. ZnO and TiO
2
nanoparticles also demonstrated immunoreactivity reduction (p < 0.05). The exception was silver nanoparticles, where the immunoreactivity increased with increasing concentration of grape extract (1.29 ± 0.01-5.47 ± 0.25 µg/g). The results confirmed the inhibitory effect of bioactive substances on the immunoreactivity of the used ELISA and also showed the need to consider immunoreactive substances when interpreting ELISA results.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abstract The effect of conventional and shock freezing on thaw drip loss and firmness of beef meat was studied. The meat samples was frozen in conventional freezer at -18 °C and shock frozen meat ...samples reached -50 °C temperature. Drip loss was measured by weighing the meat sample during thawing. The firmness was performed with TA-XT Plus Texture Analyzer with the volodkevich bite jaws. The firmness score and thaw drip loss differences did not been recognized between conventional and shock freezing meat excluding 7th day.
In this work, we study the equation of state of symmetric nuclear matter at zero temperature over a wide range of densities using two complementary theoretical approaches. At low densities, up to ...twice nuclear saturation density, we compute the energy per particle based on modern nucleon-nucleon and three-nucleon interactions derived within chiral effective field theory. For higher densities, we derive for the first time constraints in a Fierz-complete setting directly based on quantum chromodynamics using functional renormalization group techniques. We find remarkable consistency of the results obtained from both approaches as they come together in density and the natural emergence of a maximum in the speed of sound cS at supranuclear densities. The presence of this maximum appears tightly connected to the formation of a diquark gap. Notably, this maximum is observed to exceed the asymptotic value cS2=1/3 while its exact position in terms of the density cannot yet be determined conclusively.
The lateral membrane organization and phase behavior of the binary lipid mixture DMPC (1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine) - DSPC (1,2-distearoyl-sn-glycero-3-phosphatidylcholine) ...without and with incorporated gramicidin D (GD) as a model biomembrane polypeptide was studied by small-angle neutron scattering, Fourier-transform infrared spectroscopy, and by two-photon excitation fluorescence microscopy on giant unilamellar vesicles. The small-angle neutron scattering method allows the detection of concentration fluctuations in the range from 1 to 200nm. Fluorescence microscopy was used for direct visualization of the lateral lipid organization and domain shapes on a micrometer length scale including information of the lipid phase state. In the fluid-gel coexistence region of the pure binary lipid system, large-scale concentration fluctuations appear. Infrared spectral parameters were used to determine the peptide conformation adopted in the different lipid phases. The data show that the structure of the temperature-dependent lipid phases is significantly altered by the insertion of 2 to 5 mol% GD. At temperatures corresponding to the gel-fluid phase coexistence region the concentration fluctuations drastically decrease, and we observe domains in the giant unilamellar vesicles, which mainly disappear by the incorporation of 2 to 5 mol% GD. Further, the lipid matrix has the ability to modulate the conformation of the inserted polypeptide. The balance between double-helical and helical dimer structures of GD depends on the phospholipid chain length and phase state. A large hydrophobic mismatch, such as in gel phase one-component DSPC bilayers, leads to an increase in population of double-helical structures. Using an effective molecular sorting mechanism, a large hydrophobic mismatch can be avoided in the DMPC-DSPC lipid mixture, which leads to significant changes in the heterogeneous lipid structure and in polypeptide conformation.
The influence of incorporating the polypeptide gramicidin on the lateral mobility of the monoacylglyceride monoolein (MO) in its bicontinuous cubic lipid mesophases is studied applying static field ...gradient NMR. The effects of gramicidin on the topology, structure and phase behaviour of the system are characterized by small-angle x-ray scattering (SAXS) experiments. On the structural level the experiments show significant shifts in the boundaries of the various mesophases. Measurements of the translational dynamics are restricted to cubic mesophases, where the diffusion coefficients of lipid and additive are determined both by geometrical obstruction and by lipid-protein interaction effects.
Nowadays is it a common practice to add vegetable protein in the production of meat products. Because of the possible substitution of high-quality raw meat with vegetable protein without the ...labelling the product package and because of the allergenic potential of many vegetable proteins, it is important to develop accurate methods for its detection. The objective of the study was to compare histochemical, immunochemical (ELISA, ALERT gliadin screening test) and immunohistochemical methods for the detection of wheat protein in meat samples and sausages. Histochemical methods were useful for the detection of flour in meat samples, but the immunohistochemical method was better for the detection of wheat protein. ALERT gliadin screening test detected gliadin from 10 mg kg⁻¹, while an immunohistochemical method detected wheat protein concentrations from 1 g kg⁻¹ and an ELISA method detected wheat protein concentrations from 4 g kg⁻¹. ALERT gliadin screening test showed results within 1 day, whilst an ELISA detection method took 2 days, and an immunohistochemical procedure took 5 days at the soonest, all including sample preparation. This study also focused on optimisation of an immunohistochemical method for samples of cooked sausage. In addition, three samples were sufficient for wheat protein detection at a concentration of 1 g kg⁻¹ (and greater) with a confidence level greater than 95%.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Immunohistochemical detection of soya protein - optimisation and verification of the method Pospiech, M.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarniho Lekarstvi; Tremlova, B.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarniho Lekarstvi; Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) ...
Czech Journal of Food Sciences,
01/2009, Letnik:
27, Številka:
1
Journal Article
Recenzirano
Odprti dostop
A functional immunohistochemical method for soya proteins detection was developed. The procedure is based on the avidin-biotin complex method that attains sufficient sensitivity. The method was ...verified by the analysis of the model samples of different forms of soya additives containing various concentrations of soya isolate. The detection limit of soya present in the model samples was 0.5%. Different possibilities of the background staining were tested. The best results were obtained with the background staining according to Calleja. The results were confirmed by the accredited indirect ELISA method. The method allows the identification of various forms of soya proteins such as isolates, texturates, concentrates, and flour.
Determination of soya protein in model meat products using image analysis Randulova, Z.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie; Tremlova, B.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie; Rezacova-Lukaskova, Z.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie ...
Czech Journal of Food Sciences,
01/2011, Letnik:
29, Številka:
4
Journal Article
Recenzirano
Odprti dostop
The aim of our study was to confirm the possibility of image analysis application as a method to quantify soy protein in meat products. Model meat products with the addition of known amounts of soy ...protein in various forms were used in our experiment. The known data were compared with the results of image analyses and the relations expressed as correlation coefficients. The two sets of data were in good agreement as the correlation coefficients varied from 0,81 to 1,00. In conclusion, image analysis is a good method to determine the amount of soy protein in meat products.
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