Several different basis functions have been used to represent the Earth's gravity field in order to generate estimates of mass variations on Earth from the analysis of data of the Gravity Recovery ...and Climate Experiment (grace) and its successor grace Follow‐On missions, including spherical harmonics, mass concentration elements (mascons) and slepian functions. Each approach depends inherently upon accurate modeling of the orbits of the pair of satellites as they revolve around the Earth, so that the observations of inter‐satellite changes in range (or, more specifically, range rate) can be exploited to identify mass variations. We have developed software using a classical orbit modeling approach, mascons and 24‐hr orbit integration, to estimate simultaneously corrections to orbital parameters and the temporal gravity field from grace data. Rather than using the range rate, we use the range acceleration as the inter‐satellite observable as it aids in localizing the mass variations. Level‐1 B range acceleration observations contain high levels of high‐frequency noise that inhibits their usefulness for this purpose. Instead, we generate range acceleration observations by numerical differentiation of the Level‐1B range rate prefit residuals. Simulations show that the gravity signal is not attenuated in this process. Our monthly estimates of mass anomalies from grace data (2003–2016) agree well with previous studies, both spatially and temporally. When converted to spherical harmonics our time series of C2,0, derived from grace data alone, are close to the independent estimates from satellite laser ranging, but the overall solution is improved by substituting the SLR C2,0.
Plain Language Summary
The Gravity Recovery and Climate Experiment (grace) space gravity mission consists of a pair of identical twin satellites one following the other and separated by about 200 km. The distance between the satellites is altered by mass distribution on Earth. While all published temporal gravity field models are estimated from the rate of change of the inter‐satellite distances (known as range rate), this study uses the relative acceleration (known as range acceleration) between the satellites as the observation to determine the distribution of mass on Earth. The methodology we developed has multiple advantages with respect to solutions derived using the range rate. Mass changes on Earth are located spatially with greater accuracy and have a better resolution of the long wavelength gravity field.
Key Points
Mass anomalies are derived from GRACE inter‐satellite range acceleration observations
High levels of high‐frequency noise present in the Level‐1B range acceleration observation can be mitigated with appropriate pre‐processing
Our solutions on major ice sheets, the Amazon basin and over oceans derived with the range acceleration agree with other processing centers
Undernutrition is common in patients admitted with stroke. We aimed to establish whether the timing and route of enteral tube feeding after stroke affected patients' outcomes at 6 months.
The FOOD ...trials consist of three pragmatic multicentre randomised controlled trials, two of which included dysphagic stroke patients. In one trial, patients enrolled within 7 days of admission were randomly allocated to early enteral tube feeding or no tube feeding for more than 7 days (early versus avoid). In the other, patients were allocated percutaneous endoscopic gastrostomy (PEG) or nasogastric feeding. The primary outcome was death or poor outcome at 6 months. Analysis was by intention to treat.
Between Nov 1, 1996, and July 31, 2003, 859 patients were enrolled by 83 hospitals in 15 countries into the early versus avoid trial. Early tube feeding was associated with an absolute reduction in risk of death of 5·8% (95% CI −0·8 to 12·5, p=0·09) and a reduction in death or poor outcome of 1·2% (−4·2 to 6·6, p=0·7). In the PEG versus nasogastric tube trial, 321 patients were enrolled by 47 hospitals in 11 countries. PEG feeding was associated with an absolute increase in risk of death of 1·0% (−10·0 to 11·9, p=0·9) and an increased risk of death or poor outcome of 7·8% (0·0 to 15·5, p=0·05).
Early tube feeding might reduce case fatality, but at the expense of increasing the proportion surviving with poor outcome. Our data do not support a policy of early initiation of PEG feeding in dysphagic stroke patients.
The absence of dystrophin at the muscle membrane leads to Duchenne muscular dystrophy (DMD), a severe muscle-wasting disease that is inevitably fatal in early adulthood. In contrast, ...dystrophin-deficient mdx mice appear physically normal despite their underlying muscle pathology. We describe mice deficient for both dystrophin and the dystrophin-related protein utrophin. These mice show many signs typical of DMD in humans: they show severe progressive muscular dystrophy that results in premature death, they have ultrastructural neuromuscular and myotendinous junction abnormalities, and they aberrantly coexpress myosin heavy chain isoforms within a fiber. The data suggest that utrophin and dystrophin have complementing roles in normal functional or developmental pathways in muscle. Detailed study of these mice should provide novel insights into the pathogenesis of DMD and provide an improved model for rapid evaluation of gene therapy strategies.
There has been growing interest in the role of viral infections and their association with adverse pregnancy outcomes. However, little is known about the impact viral infections have on the fetal ...membranes (FM). Toll-like receptors (TLR) are thought to play a role in infection-associated inflammation at the maternal-fetal interface. Therefore, the objective of this study was to characterize the cytokine profile and antiviral response in human FMs exposed to viral dsRNA, which activates TLR3, and viral ssRNA, which activates TLR8; and to determine the mechanisms involved. The viral dsRNA analog, Poly(I:C), induced up-regulated secretion of MIP-1α, MIP-1β, RANTES and TNF-α, and down-regulated interleukin (IL)-2 and VEGF secretion. In contrast, viral ssRNA induced a broader panel of cytokines in the FMs by up-regulating the secretion of IL-1β, IL-2, IL-6, G-CSF, MCP-1, MIP-1α, MIP-1β, RANTES, TNF-α and GRO-α. Using inhibitory peptides against TLR adapter proteins, FM secretion of MIP-1β and RANTES in response to Poly(I:C) was MyD88 dependent; MIP-1α secretion was dependent on MyD88 and TRIF; and TNF-α production was independent of MyD88 and TRIF. Viral ssRNA-induced FM secretion of IL-1β, IL-2, IL-6, G-CSF, MIP-1α, RANTES and GRO-α was dependent on MyD88 and TRIF; MIP-1β was dependent upon TRIF, but not MyD88; and TNF-α and MCP-1 secretion was dependent on neither. Poly(I:C), but not ssRNA, induced an FM antiviral response by up-regulating the expression of IFNβ, myxovirus-resistance A, 2',5'-oligoadenylate synthetase and apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G. These findings demonstrate that human FMs respond to two viral signatures by generating distinct inflammatory cytokine/chemokine profiles and antiviral responses through different mechanisms.
Nine ecosystem process models were used to predict CO2 and water vapor exchanges by a 150‐year‐old black spruce forest in central Canada during 1994–1996 to evaluate and improve the models. Three ...models had hourly time steps, five had daily time steps, and one had monthly time steps. Model input included site ecosystem characteristics and meteorology. Model predictions were compared to eddy covariance (EC) measurements of whole‐ecosystem CO2 exchange and evapotranspiration, to chamber measurements of nighttime moss‐surface CO2 release, and to ground‐based estimates of annual gross primary production, net primary production, net ecosystem production (NEP), plant respiration, and decomposition. Model‐model differences were apparent for all variables. Model‐measurement agreement was good in some cases but poor in others. Modeled annual NEP ranged from −11 g C m−2 (weak CO2 source) to 85 g C m−2 (moderate CO2 sink). The models generally predicted greater annual CO2 sink activity than measured by EC, a discrepancy consistent with the fact that model parameterizations represented the more productive fraction of the EC tower “footprint.” At hourly to monthly timescales, predictions bracketed EC measurements so median predictions were similar to measurements, but there were quantitatively important model‐measurement discrepancies found for all models at subannual timescales. For these models and input data, hourly time steps (and greater complexity) compared to daily time steps tended to improve model‐measurement agreement for daily scale CO2 exchange and evapotranspiration (as judged by root‐mean‐squared error). Model time step and complexity played only small roles in monthly to annual predictions.
This paper describes the use of satellite data to calibrate a new climate vegetation greenness relation for global change studies. We examined statistical relations between annual climate indexes ...(temperature, precipitation, and surface radiation) and seasonal attributes of the AVHRR Normalized Difference Vegetation Index (NDVI) time series for the mid-1980s in order to refine our understanding of intra-annual patterns and global controls on natural vegetation dynamics. Multiple linear regression results using global 1 gridded data sets suggest that three climate indexes: degree days (growing/chilling), annual precipitation total, and an annual moisture index together can account to 70-80% of the geographical variation in the NDVI seasonal extremes (maximum and minimum values) for the calibration year 1984. Inclusion of the same annual climate index values from the previous year explains no substantial additional portion of the global scale variation in NDVI seasonal extremes. The monthly timing of NDVI extremes is closely associated with seasonal patterns in maximum and minimum temperature and rainfall, with lag times of 1 to 2 months. We separated well-drained areas from 1 grid cells mapped as greater than 25% inundated coverage for estimation of both the magnitude and timing of seasonal NDVI maximum values. Predicted monthly NDVI, derived from our climate-based regression equations and Fourier smoothing algorithms, shows good agreement with observed NDVI for several different years at a series of ecosystem test locations from around the globe. Regions in which NDVI seasonal extremes are not accurately predicted are mainly high latitude zones, mixed and disturbed vegetation types, and other remote locations where climate station data are sparse.
Pharmacological studies of narcoleptic canines indicate that exaggerated pontine cholinergic transmission promotes cataplexy. As disruption of orexin (hypocretin) signaling is a primary defect in ...narcolepsy with cataplexy, we investigated whether markers of cholinergic synaptic transmission might be altered in mice constitutively lacking orexin receptors (double receptor knockout; DKO). mRNA for Choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT) and the high‐affinity choline transporter (CHT1) but not acetylcholinesterase (AChE) was significantly higher in samples from DKO than wild‐type (WT) mice. This was region‐specific; levels were elevated in samples from the laterodorsal tegmental nucleus (LDT) and the fifth motor nucleus (Mo5) but not in whole brainstem samples. Consistent with region‐specific changes, we were unable to detect significant differences in Western blots for ChAT and CHT1 in isolates from brainstem, thalamus and cortex or in ChAT enzymatic activity in the pons. However, using ChAT immunocytochemistry, we found that while the number of cholinergic neurons in the LDT and Mo5 were not different, the intensity of somatic ChAT immunostaining was significantly greater in the LDT, but not Mo5, from DKO than from WT mice. We also found that ChAT activity was significantly reduced in cortical samples from DKO compared with WT mice. Collectively, these findings suggest that the orexins can regulate neurotransmitter expression and that the constitutive absence of orexin signaling results in an up‐regulation of the machinery necessary for cholinergic neurotransmission in a mesopontine population of neurons that have been associated with both normal rapid eye movement sleep and cataplexy.
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