Objective:To formulate a simple rapid procedure for bioreduction of silver nanoparticles using aqueous leaves extract of Moringa oleifera（M.oleifera）.Methods:10 mL of leaf extract was mixed to 90 mL ...of 1 mM aqueous of AgNO3 and was heated at 60-80 ℃ for 20 min.A change from brown to reddish color was observed.Characterization using UV-Vis spectrophotometry, Transmission Electron Microscopy（TEM） was performed.Results:TEM showed the formation of silver nanoparticles with an average size of 57 nm.Conclusions:M.oleifera demonstrates strong potential for synthesis of silver nanoparticles by rapid reduction of silver ions（Ag+ to Ag0）. Biological methods are good competents for the chemical procedures,which are eco-friendly and convenient.
Nanotechnology is gaining momentum due to its ability to transform metals into nanoparticles. The synthesis, characterization, and applications of biologically synthesized nanomaterials have become ...an important branch of nanotechnology. Plant extracts are a cost-effective, ecologically friendly, and efficient alternative for the large-scale synthesis of nanoparticles. In this study, silver nanoparticles (AgNps) were synthesized using Rhinacanthus nasutus leaf extract. After exposing the silver ions to the leaf extract, the rapid reduction of silver ions led to the formation of AgNps in solution. The synthesis was confirmed by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy. The in vitro antimicrobial activity of the AgNps synthesized using R. nasutus leaf extract was investigated against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Aspergillus niger, and Aspergillus flavus using a disc diffusion method. The AgNps showed potential activity against all of the bacterial strains and fungal colonies, indicating that R. nasutus has the potential to be used in the development of value-added products in the biomedical and nanotechnology-based industries.
•First report on soapnut extract mediated synthesis of nanoscale Cobalt substituted NdFeB ferromagnetic materials.•Biogenic magnetic nanomaterials exhibited enhanced magnetic properties compared to ...their bulk counterparts.•Eco-friendly and cheaper method to produce highly efficient ferromagnetic materials with anticipated potential industrial applications.
Neodymium iron boron (NdFeB) permanent magnets have high energy product with suitable magnetic and physical properties for an array of applications including power generation and motors. However, synthetic routes of NdFeB permanent magnets involve critical procedures with high energy and needs scientific skills. Herein, we report on soapnut extract mediated synthesis of nanoscale cobalt substituted NdFeB (Co-NdFeB) permanent magnetic powders (Nd: 15%, Fe: 77.5%, B: 7.5% and Co with molar ratios: 0.5, 1, 1.5 and 2). A 10 ml of 10% soapnut extract was added to 90 ml of respective chemical composition and heated to 60 °C for 30 min and aged for 24 h. The dried powder was sintered at 500 °C for 1 h. The characterization of the prepared nanoscale Co-NdFeB magnetic powders was done using the techniques such as Dynamic Light Scattering (DLS for size and zeta potential measurements), X-ray diffraction (XRD) for structural determination, Scanning electron microscopy (SEM) with energy dispersion spectroscopy (EDS) for surface morphological and elemental analysis, Fourier transform infrared spectroscopy (FT-IR) for the identification of functional groups associated and hysteresis loop studies to quantify the magnetization. The results revealed that particles were in irregular and tubular shaped and highly stable (Zeta potential: −44.4 mV) with measured size <100 nm. XRD micrographs revealed a tetragonal crystal structure and FTIR showed predominant N–H and O–H stretching indicates the involvement of these functional groups in the reduction and stabilization process of Co-NdFeB magnetic powders. Hysteresis studies signify the effect of an increase in Co concentration.
Synthesis of metal nanoparticles using biological systems is an expanding research area in nanotechnology. Moreover, search for new nanoscale antimicrobials is been always attractive as they find ...numerous avenues for application in medicine. Biosynthesis of metallic nanoparticles is cost effective and eco-friendly compared to those of conventional methods of nanoparticles synthesis. Herein, we present the synthesis of zinc oxide nanoparticles using the stem bark extract of
Boswellia ovalifoliolata,
and evaluation of their antimicrobial efficacy. Stable ZnO nanoparticles were formed by treating 90 ml of 1 mM zinc nitrate aqueous solution with 10 ml of 10 % bark extract. The formation of
B. ovalifoliolata
bark-extract-mediated zinc oxide nanoparticles (BZnNPs) was confirmed by UV–visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 230 nm. Fourier transform infrared spectroscopic (FT-IR) analysis revealed that primary and secondary amine groups in combination with the proteins present in the bark extract are responsible for the reduction and stabilization of the BZnNPs. The morphology and crystalline phase of the nanocrystals were determined by Transmission electron microscopy (TEM). The hydrodynamic diameter (20.3 nm) and a positive zeta potential (4.8 mV) were measured using the dynamic light scattering technique. The antimicrobial activity of BZnNPs was evaluated (in vitro) against fungi, Gram-negative, and Gram-positive bacteria using disk diffusion method which were isolated from the scales formed in drinking water PVC pipelines.
Background: Hyperlipidemia is the disorder of lipid metabolism, characterized by elevated serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and very ...low-density lipoproteins cholesterol (VLDL-C) and decreased high-density lipoprotein cholesterol (HDL-C). Atherosclerosis is a cardiovascular and fibroproliferative inflammatory disease commonly associated with dyslipidemia.Methods: The study was conducted using 24 Wistar male rats, divided into two groups of 12 rats each. Hyperlipidemia and atherosclerosis were induced by the addition of 1% cholesterol and 15% saturated oil to the 1000g of standard rat diet and given to group II rats. Group I kept as control and maintained for 90 days. Result: The present experiment established hyperlipidemia with a significant increase of TC, TG, LDL-C and decreased HDL-C cholesterol with initiated atherosclerotic lesions in the aorta and showed varying degrees of positivity with CD31, CD44, Cadherin and Beta-catenin proteins.
Nanobiotechnology, the bio-branch of nanotechnology is considered to be one of the fastest emerging research fields. Biosynthesis of metallic nanoparticles is currently under exploitation. Use of ...plant and plant materials for the synthesis of Zinc nanoparticles is relatively new and exciting research field. The biogenic zinc nanoparticles were synthesized using the leaves of
Parthenium hysterophorous
by green synthesis route. UV–VIS absorption spectroscopy was used to monitor the quantitative formation of zinc nanoparticles. The characteristics of the synthesized zinc nanoparticles were studied using scanning electron microscopy and nanoparticle analyzer. Zinc nanoparticles were observed to be spherical in shape with size range of 16 to 108.5 nm. The measured zeta potentials varied from 100.4 to 117.20 mV indicate high dispersion of the zinc nanoparticles. The synthesized zinc nanoparticles showed good enzymatic activity and microbial activity. The physiological parameters increased from 30 to 60 days of sowing when compared to control.
Background: Atherosclerosis is diverse disease with heterogenous mechanisms of progression and is an oxidative, chronic inflammatory and thrombotic disease produced by elevated level of low density ...lipoprotein cholesterol (LDL-C) in the blood. Initiation of atherosclerosis begins with the production of various inflammatory cytokines and adhesive molecules on the endothelium of the large muscular arteries like aorta. The present study was carried out to know the expression of TNF-Alpha and IL-18 by real time PCR (Polymerase chain reaction).
Methods: Procured 24 Wistar albino rats and divided into two groups consisting of 12 rats in each. Atherosclerosis was induced by addition of 1% cholesterol and 15% saturated oil to the 1000g of standard rat diet and given to group II rats. Group I kept as control and maintained for 90 days.
Result: Group II rats clinically showed obesity with significant increase (p less than 0.05) in the body weight. TC, TG, LDL-C, VLDL-C were significantly (p less than 0.05) higher, whereas HDL-C was significantly (p less than 0.05) reduced. Atherogenic diet induced the initiation of atherosclerosis in the aorta with degeneration of endothelial cells, sub intimal lipid laden macrophages (foam cells), slight thickening of the tunica intima with proliferation of few SMCs in group I rats. Present study revealed positive expression of TNF- alpha and no expression of IL-18 cytokine in the aorta of group II rats by RT-PCR.
The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. Herein, we report on rapid biosynthesis of ...silver nanoparticles (SNPs) from aqueous stem bark extract of
Cochlospermum religiosum
a medicinal plant. The reduced silver nanoparticles were characterized by using UV–Visible spectroscopy (UV–Vis), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray analysis, atomic force microscopy, and Fourier transform infrared (FT-IR). The UV–Visible spectrum of the aqueous medium containing silver nanoparticles showed an absorption peak at around 445 nm, XRD showed that the particles are crystalline in nature, with a face-centered cubic structure and the SEM images showed that the spherical-shaped silver nanoparticles were observed and the size range was found to be 20–35 nm. FT-IR spectroscopy analysis revealed that carbohydrate, polyphenols, and protein molecules were involved in the synthesis and capping of silver nanoparticles. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone.
Cochlospermum religiosum
aqueous stem bark extract of SNPs showed highest toxicity to
Staphylococcus
followed by
Pseudomonas
,
Escherichia coli
and
Bacillus
and lowest toxicity towards
Proteus
. Whereas in fungal species highest inhibition zone against
Aspergillus flavus
followed by
Rhizopus,
Fusarium,
and
Curvularia,
and minimum inhibition zone was observed against
Aspergillus niger
species. The outcome of this study could be useful for the development of value added products from indigenous medicinal plants of India for nanotechnology-based biomedical applications.
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