The planetary boundary layer (PBL) height is a key parameter in air quality control and pollutant dispersion. The PBL height cannot, however, be directly measured, and its estimation relies on the ...analysis of the vertical profiles of the temperature, turbulence or the atmospheric composition. An operational PBL height detection method including several remote sensing instruments (wind profiler, Raman lidar, microwave radiometer) and several algorithms (Parcel and bulk Richardson number methods, surface-based temperature inversion, aerosol or humidity gradient analysis) was developed and tested with 1 year of measurements, which allows the methods to be validated against radio sounding measurements. The microwave radiometer provides convective boundary layer heights in good agreement with the radio sounding (RS) (median bias < 25 m, R2 > 0.70) and allows the analysis of the diurnal variation of the PBL height due to its high temporal resolution. The Raman lidar also leads to a good agreement with RS, whereas the wind profiler yields some more dispersed results mostly due to false attribution problems. A comparison with the numerical weather prediction model COSMO-2 has shown a general overestimation of the model PBL height by some hundreds to thousand meters. Finally the seasonal cycles of the daytime and nighttime PBL heights are discussed for each instrument and each detection algorithm for two stations on the Swiss plateau.
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•A phylogeny based on 6 genes and morphology is presented for a large complex of eucerine bees.•A new generic-level classification is proposed for this systematically complex group of ...pollinators.•The evolution of morphological traits is analyzed relative to the molecular phylogeny.•The use of functional traits as diagnostic characters in classifications is critically discussed.•A biogeographic hypothesis explaining the wide distribution of this clade is added.
The longhorn bee tribe Eucerini (Hymenoptera: Apidae) is a diverse, widely distributed group of solitary bees that includes important pollinators of both wild and agricultural plants. About half of the species in the tribe are currently assigned to the genus Eucera and to a few other related genera. In this large genus complex, comprising ca. 390 species, the boundaries between genera remain ambiguous due to morphological intergradation among taxa. Using ca. 6700 aligned nucleotide sites from six gene fragments, 120 morphological characters, and more than 100 taxa, we present the first comprehensive molecular, morphological, and combined phylogenetic analyses of the ‘Eucera complex’. The revised generic classification that we propose is congruent with our phylogeny and maximizes both generic stability and ease of identification. Under this new classification most generic names are synonymized under an expanded genus Eucera. Thus, Tetralonia, Peponapis, Xenoglossa, Cemolobus, and Syntrichalonia are reduced to subgeneric rank within Eucera, and Synhalonia is retained as a subgenus of Eucera. Xenoglossodes is reestablished as a valid subgenus of Eucera while Tetraloniella is synonymized with Tetralonia and Cubitalia with Eucera. In contrast, we suggest that the venusta-group of species, currently placed in the subgenus Synhalonia, should be recognized as a new genus. Our results demonstrate the need to evaluate convergent loss or gain of important diagnostic traits to minimize the use of potentially homoplasious characters when establishing classifications. Lastly, we show that the Eucera complex originated in the Nearctic region in the late Oligocene, and dispersed twice into the Old World. The first dispersal event likely occurred 24.2–16.6 mya at a base of a clade of summer-active bees restricted to warm region of the Old World, and the second 13.9–12.3 mya at the base of a clade of spring-active bees found in cooler regions of the Holarctic. Our results further highlight the role of Beringia as a climate-regulated corridor for bees.
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•A phylogeny based on 5 genes is presented for the species-rich bee tribe Megachilini.•A new generic-level classification is proposed for this important group of pollinators.•A ...biogeographic scenario for this cosmopolitan bee group is inferred.•DNA barcodes are combined to a nuclear backbone phylogeny to increase taxon sampling.
Classification and evolutionary studies of particularly speciose clades pose important challenges, as phylogenetic analyses typically sample a small proportion of the existing diversity. We examine here one of the largest bee genera, the genus Megachile – the dauber and leafcutting bees. Besides presenting a phylogeny based on five nuclear genes (5480 aligned nucleotide positions), we attempt to use the phylogenetic signal of mitochondrial DNA barcodes, which are rapidly accumulating and already include a substantial proportion of the known species diversity in the genus. We used barcodes in two ways: first, to identify particularly divergent lineages and thus to guide taxon sampling in our nuclear phylogeny; second, to augment taxon sampling by combining nuclear markers (as backbone for ancient divergences) with DNA barcodes. Our results indicate that DNA barcodes bear phylogenetic signal limited to very recent divergences (3–4 my before present). Sampling within clades of very closely related species may be augmented using this technique, but our results also suggest statistically supported, but incongruent placements of some taxa. However, the addition of one single nuclear gene (LW-rhodopsin) to the DNA barcode data was enough to recover meaningful placement with high clade support values for nodes up to 15 million years old. We discuss different proposals for the generic classification of the tribe Megachilini. Finding a classification that is both in agreement with our phylogenetic hypotheses and practical in terms of diagnosability is particularly challenging as our analyses recover several well-supported clades that include morphologically heterogeneous lineages. We favour a classification that recognizes seven morphologically well-delimited genera in Megachilini: Coelioxys, Gronoceras, Heriadopsis, Matangapis, Megachile, Noteriades and Radoszkowskiana. Our results also lead to the following classification changes: the groups known as Dinavis, Neglectella, Eurymella and Phaenosarus are reestablished as valid subgenera of the genus Megachile, while the subgenus Alocanthedon is placed in synonymy with M. (Callomegachile), the subgenera Parachalicodoma and Largella with M. (Pseudomegachile), Anodonteutricharaea with M. (Paracella), Platysta with M. (Eurymella), and Grosapis and Eumegachile with M. (Megachile) (new synonymies). In addition, we use maximum likelihood reconstructions of ancestral geographic ranges to infer the origin of the tribe and reconstruct the main dispersal routes explaining the current, cosmopolitan distribution of this genus.
A proof‐of‐concept of space‐borne laser filamentation for atmospheric remote sensing is presented. The remote generation of laser filaments from an Earth‐orbiting satellite is shown by numerical ...simulations to be theoretically possible for a large range of laser parameters. The model includes a realistic representation of the stratified atmosphere and accounts for multi‐species ionization and the dependence of air density upon the molecule type and altitude profile. The remote generation of a white light continuum extending from 350 nm to 1.1 μm within the filament is demonstrated, and hereby proposed as an atmospheric in‐situ light source for monitoring greenhouse gases and pollutants on a global scale by light detection and ranging (lidar) techniques. Scaling laws are also derived for estimating the filament altitude as a function of peak pulse power (3 GW‐3 TW), beam radii (10‐200 cm) and for three different curvatures (300, 390, 500 km) for femtosecond infrared (800 nm) pulses. We find that operating conditions for remote supercontinuum generation are already available with current ground‐based mobile laser technology and within reach of future space laser systems.
Remote sensing of the atmosphere via an Earth‐orbiting satellite hosting a femtosecond pulse laser would provide attractive information in the field of climate science and atmospheric chemistry. This study provides a proof of concept for spaceborne filamentation and white light supercontinuum generation in the atmosphere. Operating conditions for spaceborne femtosecond LIDAR are found to be available with current ground‐based mobile laser technology and within reach of future space laser systems.
Within the Hymenoptera, bees are notable for their relationship with flowering plants, being almost entirely dependent on plant pollen and nectar. Though functionally herbivorous, as a result of ...their role as pollinators, bees have received comparatively little attention as models for insect herbivory. Bees often display dietary specialization, but quantitative comparison against other herbivorous insects has not previously been conducted.
In the most comprehensive analysis to date for 860 bee species, dietary specialization amounted to 50.1% of studied species collecting pollen from between 1 and 2 botanical families with a relatively long tail of dietary generalists, with 11.1% of species collecting from more than 10 botanical families. This distribution deviated from the truncated Pareto distribution of dietary breadth seen in other herbivorous insect lineages. However, this deviation was predominantly due to eusocial bee lineages, which show a range of dietary breadths that conformed to a normal distribution, while solitary bees show a typical truncated distribution not strongly different from other herbivorous insects. We hypothesize that the relatively low level of dietary specialization in bees as a whole reflects the relaxation of the constraints typically observed in herbivorous insects with a comparatively reduced importance of plant chemistry and comparatively increased importance of phenology and foraging efficiency. The long flight periods of eusocial bees that are necessary to allow overlapping generations both allows and necessitates the use of multiple flowering resources, whereas solitary bees with short flight periods have more limited access to varied resources within a constrained activity period.
Collectively, solitary bees show slightly lower specialization compared to other herbivorous insects, possibly due to their balanced relationship with plants, rather than direct antagonism such as seen in the direct consumption of plant tissues. An additional factor may be the mediocre diversity of bees at low latitudes combined with low levels of dietary specialization, whereas these areas typically display a high rate of specialization by herbivorous insects in general. Though the most important factors structuring dietary specialization in bees appear to differ from many other herbivorous insects, solitary bees show a surprisingly similar overall pattern of dietary specialization.
Abstract We present a measurement of the Cabibbo-Kobayashi-Maskawa unitarity triangle angle ϕ 3 (also known as γ) using a model-independent Dalitz plot analysis of B + → D ( K S 0 $$ {K}_S^0 $$ h + h ...− )h +, where D is either a D 0 or D ¯ $$ \overline{D} $$ 0 meson and h is either a π or K. This is the first measurement that simultaneously uses Belle and Belle II data, combining samples corresponding to integrated luminosities of 711 fb −1 and 128 fb −1, respectively. All data were accumulated from energy-asymmetric e + e − collisions at a centre-of-mass energy corresponding to the mass of the Υ(4S) resonance. We measure ϕ 3 = (78.4 ± 11.4 ± 0.5 ± 1.0)°, where the first uncertainty is statistical, the second is the experimental systematic uncertainty and the third is from the uncertainties on external measurements of the D-decay strong-phase parameters.
Durable resistance against fungal pathogens is highly valuable for disease management in agriculture. For its sustainable use, and to avoid pathogen adaptation, it is important to understand the ...underlying molecular mechanisms. Many studies on durable disease resistance in plants have focused exclusively on the host plant, whereas possible reactions and adaptations of pathogens exposed to this type of resistance have not been well researched. The wheat Lr34 gene, encoding a putative ABC‐transporter, provides broad‐spectrum and durable resistance against multiple fungal pathogens in wheat and is functional as a transgene in all major cereals. Lr34‐based resistance is partial, meaning pathogens can grow and reproduce to some degree on Lr34‐containing plants. Therefore, Lr34‐expressing plants are ideal for studying the response of pathogens to partial resistance. Here, transcriptomic responses of the two fungal pathogens Blumeria graminis f. sp. hordei (barley powdery mildew) and Puccinia triticina (wheat leaf rust) during growth on their respective host plants containing Lr34 were compared to their responses on control plants without Lr34. Two different time points after inoculation were chosen for analysis of powdery mildew on barley and one time point for wheat leaf rust. Transcriptome analyses revealed that there were no differences in the expression patterns of the two pathogens growing on susceptible versus partially resistant plants, even though pathogen growth was reduced in the presence of Lr34. This reflects the absence of observable reaction in the pathogen to the presence of the Lr34 resistance gene and, consequently, no major alteration of fungal pathogen metabolism.
The profession of the health-care providers (HCPs) influences their recommendations to the patients. Conversely, interdisciplinarity seeks to challenge such differences, so that the patient receives ...one single and consistent therapeutic message. Some studies also suggest associations between HCPs life habits and recommendations. Our hypotheses were (1) that despite interdisciplinary work, the profession remains a predictor of recommendations and (2) that HCPs who are more physically active recommend more activity. Three clinical vignettes were presented to a group of experts of low back pain (LBP) (guidelines), and 20 physicians, 22 physiotherapists, and 23 nurses to assess how they evaluate the symptoms and pathologies of LBP patients and how much work and physical activity they recommend. Physical activity was assessed with accelerometers and questionnaires. Some interprofessional differences remained present within an interdisciplinary team. The nurses were more restrictive and further away from the guidelines. The physicians were the most in line with them. The physiotherapists recommend as much physical activity, but less work activity than the physicians. The level of physical activity of the HCPs is not associated with their recommendations. To ensure a clear and unique message, educational actions may be undertaken to promote the biopsychosocial model and clarify the guidelines.
A versatile method to automatically classify ice particle habit from various airborne optical array probes is presented. The classification is achieved using a multinomial logistic regression model. ...For each airborne probe, the model determines the particle habit (among six classes) based on a large set of geometrical and textural descriptors extracted from the two‐dimensional image of a particle. The technique is applied and evaluated using three probes with significantly different specifications: the high volume precipitation spectrometer, the two‐dimensional stereo probe, and the cloud particle imager. Performance and robustness of the method are assessed using standard machine learning tools on the basis of thousands of images manually labeled for each of the considered probes. The three classifiers show good performance characterized by overall accuracies and Heidke skill scores above 90%. Depending on the application and user preferences, the classification scheme can be easily adapted. For a more precise output, intraclass subclassification can be achieved in a nested fashion, illustrated here with columnar crystals and aggregates. A comparative study of the classification output obtained with the three probes is presented for two aircraft flight periods selected when the three probes were operating together. Results are globally consistent in term of proportions of habit identified (once blurry and partial images have been automatically discarded). A perfect agreement is not expected as the three considered probes are sensitive to different particle size range.
Plain Language Summary
An automatic classification method to identify ice particle habit from images is proposed. The technique is applied and evaluated using three airborne probes mounted on research aircraft with significantly different specifications: the high volume precipitation spectrometer, the two‐dimensional stereo probe, and the cloud particle imager. The method relies on thousand of images manually classified and advanced machine learning techniques to determine the snow crystal habit among six preset classes. High classification performance is achieved, with accuracies above 90% for each of the considered probes.
Key Points
A general and versatile method to automatically identify ice particle habit from airborne probe images is proposed
The method is successfully tested on three different airborne imaging probes
Good performance with classification accuracies >90% for each probe
Bees require large amounts of pollen for their own reproduction. While several morphological flower traits are known to have evolved to protect plants against excessive pollen harvesting by bees, ...little is known on how selection to minimize pollen loss acts on the chemical composition of pollen. In this study, we traced the larval development of four solitary bee species, each specialized on a different pollen source, when reared on non-host pollen by transferring unhatched eggs of one species onto the pollen provisions of another species. Pollen diets of Asteraceae and Ranunculus (Ranunculaceae) proved to be inadequate for all bee species tested except those specialized on these plants. Further, pollen of Sinapis (Brassicaceae) and Echium (Boraginaceae) failed to support larval development in one bee species specialized on Campanula (Campanulaceae). Our results strongly suggest that pollen of these four taxonomic groups possess protective properties that hamper digestion and thus challenge the general view of pollen as an easy-to-use protein source for flower visitors.
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