As imaging techniques rapidly evolve to probe nanoscale genome organization at higher resolution, it is critical to consider how the reagents and procedures involved in sample preparation affect ...chromatin at the relevant length scales. Here, we investigate the effects of fluorescent labeling of DNA sequences within chromatin using the gold standard technique of three-dimensional fluorescence in situ hybridization (3D FISH). The chemical reagents involved in the 3D FISH protocol, specifically formamide, cause significant alterations to the sub-200 nm (sub-Mbp) chromatin structure. Alternatively, two labeling methods that do not rely on formamide denaturation, resolution after single-strand exonuclease resection (RASER)-FISH and clustered regularly interspaced short palindromic repeats (CRISPR)-Sirius, had minimal impact on the three-dimensional organization of chromatin. We present a polymer physics-based analysis of these protocols with guidelines for their interpretation when assessing chromatin structure using currently available techniques.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
We present results from a search for a radio transient associated with the LIGO/Virgo source S190814bv, a likely neutron star-black hole (NSBH) merger, with the Australian Square Kilometre Array ...Pathfinder. We imaged a 30 deg2 field at ΔT = 2, 9, and 33 days post-merger at a frequency of 944 MHz, comparing them to reference images from the Rapid ASKAP Continuum Survey observed 110 days prior to the event. Each epoch of our observations covers 89% of the LIGO/Virgo localization region. We conducted an untargeted search for radio transients in this field, resulting in 21 candidates. For one of these, AT2019osy, we performed multiwavelength follow-up and ultimately ruled out the association with S190814bv. All other candidates are likely unrelated variables, but we cannot conclusively rule them out. We discuss our results in the context of model predictions for radio emission from NSBH mergers and place constrains on the circum-merger density and inclination angle of the merger. This survey is simultaneously the first large-scale radio follow-up of an NSBH merger, and the most sensitive widefield radio transients search to-date.
Issues with Private Practice Wine, Byron; Pritchard, Joshua K.
Journal of organizational behavior management,
10/2020, Letnik:
40, Številka:
3-4
Journal Article
Recenzirano
Organizational Behavior Management (OBM) is a robust sub-discipline of behavior analysis. Practitioners apply techniques across industries with any number of goals. Given the breadth of practice, ...many questions can arise around implementing OBM services. However, there exists very little well-controlled research or other resources for practitioners to navigate the many obstacles related to practice. This paper, based largely from the experience of the authors, presents many common issues seen in practice along with recommendations that we have found to be useful.
Abstract
We present the detection of rotationally modulated, circularly polarized radio emission from the T8 brown dwarf WISE J062309.94−045624.6 between 0.9 and 2.0 GHz. We detected this ...high-proper-motion ultracool dwarf with the Australian SKA Pathfinder in 1.36 GHz imaging data from the Rapid ASKAP Continuum Survey. We observed WISE J062309.94−045624.6 to have a time and frequency averaged Stokes
I
flux density of 4.17 ± 0.41 mJy beam
−1
, with an absolute circular polarization fraction of 66.3% ± 9.0%, and calculated a specific radio luminosity of
L
ν
∼ 10
14.8
erg s
−1
Hz
−1
. In follow-up observations with the Australian Telescope Compact Array and MeerKAT we identified a multipeaked pulse structure, used dynamic spectra to place a lower limit of
B
> 0.71 kG on the dwarf’s magnetic field, and measured a
P
= 1.912 ± 0.005 hr periodicity, which we concluded to be due to rotational modulation. The luminosity and period we measured are comparable to those of other ultracool dwarfs observed at radio wavelengths. This implies that future megahertz to gigahertz surveys, with increased cadence and improved sensitivity, are likely to detect similar or later-type dwarfs. Our detection of WISE J062309.94−045624.6 makes this dwarf the coolest and latest-type star observed to produce radio emission.
Abstract
We present the detection of 661 known pulsars observed with the Australian SKA Pathfinder (ASKAP) telescope at 888 MHz as part of the Rapid ASKAP Continuum Survey (RACS). Detections were ...made through astrometric coincidence and we estimate the false alarm rate of our sample to be ∼0.5%. Using archival data at 400 and 1400 MHz, we estimate the power-law spectral indices for the pulsars in our sample and find that the mean spectral index is −1.78 ± 0.6. However, we also find that a single power law is inadequate for modeling all the observed spectra. With the addition of flux densities between 150 MHz and 3 GHz from various imaging surveys, we find that up to 40% of our sample show deviations from a simple power-law model. Using Stokes
V
measurements from the RACS data, we measured the circular polarization fraction for 9% of our sample and find that the mean polarization fraction is ∼10% (consistent between detections and upper limits). Using the dispersion-measure-derived distance, we estimate the pseudo-luminosity of the pulsars and do not find any strong evidence for a correlation with the pulsars’ intrinsic properties.
We examined publicly available faculty salaries for men and women faculty members at 16 university programs accredited by the Association for Behavior Analysis International (ABAI). Overall, 52.4% of ...the 103 faculty members were women, although there were twice as many men as women at the full-professor level. Our data suggest that ABAI-accredited training programs pay women less than men at all academic levels. Both in absolute terms and relative to the wage gap reported in other areas of psychology, the difference in mean wages for women and men in our sample was substantial. The mean salaries of men were 13%, 6%, and 15% greater than those of women at the assistant-, associate-, and full-professor levels, respectively. At all levels, the highest salary reported was earned by a man, and the lowest salary was earned by a woman. This is an embarrassment for our discipline. It is time for a change, and we behavior analysts have the tools to make change happen. Let us put those tools to good use.
As the natural science of behavior evolves, the use of precise terminology is critical to maintain its conceptual and terminological integrity. The current state of terminology in stimulus control is ...well developed with respect to reinforcement and incomplete with respect to punishment. In this paper, we aim to make the case that the current conceptualization for discriminative stimulus control in relation to punishment would be enhanced by modifying the definition of the discriminative stimulus for punishment (S
) and by adding a new term to the current taxonomy that denotes when a punishment contingency is inactive.
As the natural science of behavior evolves, the use of precise terminology is critical to maintain its conceptual and terminological integrity. The current state of terminology in stimulus control is ...well-developed with respect to reinforcement and incomplete with respect to punishment. In this paper, we aim to make the case that the current conceptualization for discriminative stimulus control in relation to punishment would be enhanced by modifying the definition of the discriminative stimulus for punishment (SDp) and by adding a new term to the current taxonomy that denotes when a punishment contingency is inactive.
Dixon, Reed, Smith, Belisle, and Jackson (
Behavior Analysis in Practice 8
, 7–15,
2015
) evaluated research productivity in BACB training programs. While research productivity may indeed be an ...important metric for future researchers, we posit that the field consists mainly of practitioners, and as such training should be focused on practice. We provide an alternative perspective on enhancing the quality of education for behavior analysts who will, by and large, go on to be practitioners.
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