Objective: The pathogenesis of leukemia is complex and there are no effective diagnostic and prognostic indicators. Previous studies showed that microRNA-22 (miR-22) has altered expression level in ...multiple leukemia subtypes, which is associated with the survival outcomes of leukemia. Methods: According to the constituted retrieval strategy, eligible studies were included from January 2010 to November 2022 by searching database. The pooled Risk Ratio (RR) and 95% confidence intervals (CI) were used to study the relationship between miR-22 and survival. Stata12.0 was used for meta-analysis. Differential expression analysis was conducted based on expression profile of miRNA. Results: Four English articles were included containing a total of 215 leukemia patients. Data showed that the pooled RR for overall survival (OS) was 1.558 (95% CI: 1.197-2.028, P < .01). Subgroup analysis for OS of acute myeloid leukemia patients and the RFS of plasma cell leukemia patients were statistically significant with different expression levels of miR-22 (RR:1.495, 95%CI:1.141-1.958, P < .01 and RR:1.517, 95%CI:1.114-2.065, P < .01, respectively). Moreover, all data included had no significant heterogeneity and publication bias. Conclusions: miR-22 is associated with the survival outcome of leukemia patients suggesting that miR-22 may be a promising prognostic biomarker for this patient population, and the expression level of miR-22 in ALL patients down-regulated.
•Aptamers not only act as an encapsulant for luminol, but as a recognition element for prostate specific antigen.•The proposed sensor is simple, convenient and no other additional complex ...pretreatment.•The proposed sensor exhibits showed good selectivity, reproducibility and stability.
Here, a target-triggered signal chemiluminescence sensor was constructed for prostate specific antigen detection based on hollow porous silica encapsulated luminol by aptamers. In this work, luminol as a chemiluminescence illuminant was encapsulated in the cavity of hollow porous silica by aptamers, and the encapsulated silicon material was prepared and characterized. Then, the encapsulated silicon material was used to construct the target-triggered signal chemiluminescence sensor. When prostate specific antigen is present, prostate specific antigen and its aptamer are specifically recognized and combine together, and aptamer is detached from the surface of silicon material. So luminol will be released, and triggers chemiluminescence reaction of luminol-H2O2, in which horseradish peroxidase acts as a catalyst. So the target-triggered signal chemiluminescence sensor was structured for prostate specific antigen detection, and the feasibility of the sensor was studied. Under optimized conditions, the sensor showed a low detection limit of 0.27 pg/mL (S/N = 3), and wide linear range of 0.001–100 ng/mL. The sensor also showed good selectivity, reproducibility and stability. The spiked recovery in serum samples that no other additional complex pretreatment ranged from 99.3%–102.3%. It provides an alternative approach to high sensitivity, selectivity and accurate detection of prostate specific antigen in actual samples.
In this work, a dual-aptamer functionalized magnetic silicon composite was prepared and used to construct a chemiluminescence (CL) sensor for the detection of α-fetoprotein (AFP) and carcinoembryonic ...antigen (CEA). First, SiO2@Fe3O4 was prepared, and polydiallyl dimethylammonium chloride (PDDA) and AuNPs were sequentially loaded on SiO2@Fe3O4. Subsequently, the complementary strand of CEA aptamer (cDNA2) and the aptamer of AFP (Apt1) were attached to AuNPs/PDDA-SiO2@Fe3O4. Then, the aptamer of CEA (Apt2) and G quadruplex peroxide-mimicking enzyme (G-DNAzyme) were sequentially connected to cDNA2, leading to the final composite. Then, the composite was used to construct a CL sensor. When AFP is present, it will combine with Apt1 on the composite to hinder the catalytic ability of AuNPs to luminol-H2O2, achieving AFP detection. When CEA is present, it will recognize and bind with Apt2, so G-DNAzyme is released to solution and catalyzes the reaction of luminol-H2O2 to achieve CEA determination. After the application of the prepared composite, AFP and CEA were detected in the magnetic medium and supernatant, respectively, after simple magnetic separation. Therefore, the detection of multiple liver cancer markers is realized through the CL technology without additional instruments or technology, which broadens the application range of CL technology. The sensor for detecting AFP and CEA shows wide linear ranges of 1.0 × 10–4 to 1.0 ng·mL–1 and 0.0001–0.5 ng·mL–1 and low detection limits of 6.7 × 10–5 ng·mL–1 and 3.2 × 10–5 ng·mL–1, respectively. Finally, the sensor was successfully used to detect CEA and AFP in serum samples and provides great potential for detection of multiple liver cancer markers in early clinical diagnosis.
In this work, a highly sensitive and selective chemiluminescence (CL) aptasensor was prepared for thrombin (THR) detection based on aptamer-conjugated and hemin/G-quadruplex DNAzyme signal-amplified ...carbon fiber composite (HG-DNAzyme/T-Apt/SiO2@GO@CF). Initially, SiO2@GO@CF was successfully prepared and characterized by Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR). Thrombin aptamer (T-Apt) as an identification element and simulated enzyme - hemin/G-quadruplex DNAzyme (HG-DNAzyme) as a signal-amplified material, were applied in the CL aptasensor. Then, the immobilization properties of SiO2@GO@CF and adsorption properties of T-Apt/SiO2@GO@CF were studied. Lastly, HG-DNAzyme/T-Apt/SiO2@GO@CF was applied in construction of the CL aptasensor. When THR existed, HG-DNAzyme was desorbed from the surface of T-Apt/SiO2@GO@CF and catalyzed the CL system of luminol-H2O2. Under optimized CL conditions, THR was measured with the linear concentration range of 1.5 × 10−14 to 2.5 × 10−11 moL/L and the detection limit of 6.3 × 10−15 moL/L (3δ). The proposed CL aptasensor was used to the determination of THR in human serum samples and recoveries ranged from 99.0% to 102.4%. Those satisfactory results illustrated the CL aptasensor could achieve highly sensitive and selective detection of THR and revealed potential application in practical samples.
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•SiO2@GO@CF was successfully prepared.•Aptamer was introduced and significantly improved the selectivity of chemiluminescence (CL) method.•G-quadruplex DNAzyme, as a signal-amplified material, improved the detection sensitivity.•The CL aptasensor was successfully used for thrombin determination in serum samples.
A novel, highly selective and sensitive chemiluminescence (CL) biosensor for insulin (INS) detection was proposed based on aptamer and oligonucleotide-gold nanoparticles functionalized nanosilica @ ...graphene oxide aerogel. Initially, nanosilica functionalized graphene oxide aerogel (SiO2@GOAG) was successfully prepared and the composite showed rich pore distribution, large specific surface area and good biocompatibility. Insulin aptamer (IGA3) was used as a biorecognition element and oligonucleotide functionalized gold nanoparticles (ssDNA-AuNPs) was used as CL signal amplification materials, which were functionalized on the surface of SiO2@GOAG. The multi-functionalized composite - ssDNA-AuNPs/IGA3/SiO2@ GOAG was obtained and used to construct the CL biosensor for insulin detection. When insulin is present in a sample, the insulin will bind to the IGA3, which will result in the release of ssDNA-AuNPs. The released ssDNA-AuNPs would catalyze the luminescence of luminol and H2O2. The linear range of the CL biosensor for insulin detection was 7.5 × 10−12 to 5.0 × 10−9 moL/L and the detection limit was 1.6 × 10−12 moL/L (S/N = 3). The selectivity and stability of the CL biosensor were also studied and the results showed that the biosensor exhibited high selectivity and good stability due to the introduction of ssDNA-AuNPs/IGA3/SiO2@GOAG. The CL biosensor was finally used for recombinant human insulin detection in recombinant human insulin injection and the results were satisfactory.
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•Multi-functioned silica @ graphene oxide aerogel composite was successfully prepared.•Aptamer was introduced to improve the selectivity of the biosensor.•Oligonucleotide-gold nanoparticle improved the sensitivity of the biosensor.•The CL biosensor was successfully used for insulin injection samples.
Two new meroterpenoids, aspergienynes O and P (1 and 2), one new natural compound, aspergienyne Q (3), and a new α-pyrone derivative named 3-(4-methoxy-2-oxo-2H-pyran-6-yl)butanoic acid (4) were ...isolated from the mangrove endophytic fungal strain Aspergillus sp. GXNU-Y85, along with five known compounds (5–9). The absolute configurations of those new isolates were confirmed through extensive analysis using spectroscopic data (HRESIMS, NMR, and ECD). The pharmacological study of the anti-proliferation activity indicated that isolates 5 and 9 displayed moderate inhibitory effects against HeLa and A549 cells, with the IC50 values ranging from 16.6 to 45.4 μM.
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•Aptamer and G-quadruplex DNAzyme modified three-dimensional graphene was successfully prepared.•The introduction of aptamer improved the selectivity of the sensor.•G-quadruplex ...DNAzyme improved the sensitivity of the sensor.•The chemiluminescence sensor was successfully used to detect streptomycin in cucumber and milk samples.
In our work, a highly selective and sensitive streptomycin (STR) chemiluminescence (CL) sensor was successfully prepared based on aptamer and G-quadruplex DNAzyme (G-DNAzyme) modified three-dimensional graphene composite. Initially, β-cyclodextrins and ionic liquids functionalized graphene oxide aerogel (β-CD/IL@GOGA) was successfully prepared and characterized, in which graphene oxide aerogel (GOGA) was used as a skeleton material and provided large specific surface area, β-CD rich in hydroxyl groups and IL containing lots of stable ions increased the biocompatibility and stability of the composite, respectively. Then, tetracycline aptamer (S-Apt) and G-DNAzyme was immobilized on the surface of β-CD/IL@GOGA, consecutively. Finally, G-DNAzyme/S-Apt/β-CD/IL@GOGA was used to construct the CL sensor for STR detection. S-Apt with specific recognition ability to its target and G-DNAzyme as a catalyst of luminol-H2O2 CL system improved the selectivity and sensitivity of the sensor, respectively. When STR existed, G-DNAzyme was released from the surface of S-Apt/β-CD/IL@GOGA due to the specific recognition and binding ability between STR and S-Apt, catalyzing the CL reaction. The CL sensor showed the linear range of 1.4 × 10−12 to 2.8 × 10−9 mol/L and detection limit of 9.2 × 10-14 mol/L (3δ). It was successfully used for STR detection in cucumber and milk samples.
Thrombin is a marker of blood-related diseases, and its detection is of great significance in the fields of medical and biological research. Herein, a novel chemiluminescence (CL) sensor for thrombin ...detection was prepared based on dual-aptamer biorecognition and mesoporous silica encapsulated with iron porphyrin. Mesoporous silica encapsulated with hematin by aptamer1 (Apt1/hematin/M-SiO2) and magnetic microspheres modified with aptamer2 (Apt2/NH2-MS) were successfully prepared, and the two materials were used to construct a CL sensor to detect thrombin. Primarily, Apt2/NH2-MS is used for pretreatment separation of thrombin samples by the biorecognition effect between the aptamer (Apt2) and target (thrombin). Then, thrombin/Apt2/NH2-MS is again recognized with Apt1 on the surface of Apt1/hematin/M-SiO2 and Apt1/thrombin/Apt2/NH2-MS is formed, so dual-aptamer biorecognition is realized. Meanwhile, the generated Apt1/thrombin/Apt2/NH2-MS makes Apt1 shed off the surface of M-SiO2 and release hematin. The released hematin can catalyze the luminol-H2O2 CL reaction. Therefore, a sandwich-type CL sensor was constructed based on dual-aptamer biorecognition and hematin catalysis for the detection of thrombin. The sensor has a linear range of 7.5 × 10–15 to 2.5 × 10–10 mol·L–1 and a detection limit of 2.2 × 10–15 mol·L–1 and also exhibits excellent selectivity, reproducibility, and stability. The sensor was successfully used for the detection of thrombin in serum samples, which makes it possible to apply the sensor in the detection of thrombin in actual samples.
In this study, a simple and rapid dispersive liquid-liquid microextraction method was established, and the residuals of four isomers of hexachlorocyclohexane and six kinds of pyrethroid pesticides in ...milk were simultaneously determined by gas chromatography electron capture detector (GC-ECD). The milk sample was first extracted with acetonitrile and cleaned with primary secondary amine (PSA). Then 0.5 mL of acetonitrile was mixed with 140 μL of cyclohexane and rapidly injected into 3 mL of pure water. After vortexing and centrifugation, the floating phase was removed with a 0.1-mL pipette into the GC-ECD. The type and volume of extraction solvent, volume of disperser solvent, volume of water, vortex time, and amount of salt were optimized. Under optimal extraction conditions, the ten pesticides showed a good linear relationship in a certain concentration range in milk matrix, and the correlation coefficients were greater than 0.99. The limits of detection ranged from 0.07 to 2 μg/kg, and the limits of quantitation ranged from 0.2 to 5 μg/kg. The average recovery rates were between 70.1% and 106.3%, and the relative standard deviations were less than 15.2%. This method can be used for the determination of hexachlorocyclohexane and pyrethroid pesticides in milk and for subsequent research.
18-facet polyhedron Cu7S4 nanocrystal and CuS sphere were prepared from Cu2O precursor, and CuS flower was synthesized through a simple solvothermal approach. Their electrochemical performances were ...investigated towards H2O2 and it was interesting to discover that Cu7S4 nanocrystal had the best electrochemical catalysis compared with CuS sphere and CuS flower. It can deduce that the special structure of Cu7S4 nanocrystal endowed it more exposed active points, higher surface area and higher Cu/S ratio. Therefore, Cu7S4 nanocrystal was firstly employed to prepare a nonenzymatic biosensor for H2O2. Satisfactory results were obtained. In addition, a label-free sensing platform for prostate specific antigen (PSA) was constructed based on electrochemical catalysis towards H2O2 of Cu7S4 nanocrystal. The label-free immunosenosr offered accurate PSA in the range of 0.001–15 ng/mL with the detection limit of 0.001 ng/mL. Besides, the immunosensor possessed good sensitivity, selectivity and stability and could detect PSA in real sample. More importantly, this work demonstrated that Cu7S4 nanocrystal hold great promising application in electrochemical sensors.
•A label-free electrochemical immunosensor for PSA detection is constructed.•18-facet polyhedron Cu7S4 nanocrystals was obtained through a simple solvothermal approach.•A biosensor for H2O2 is constructed based on 18-facet polyhedron Cu7S4 nanocrystals.•The immunosensor has been used to detect PSA in human serum sample.
