Abstract
Cholesterol is a type of lipid found in the body that aids in the digestion of food and is required for the formation of hormones. Even yet, increased levels in the blood can raise the risk ...of coronary heart attacks. Medicinal plants, in addition to pharmacological therapy, can be used to prevent levels from rising. Lemongrass (Cymbopogon nardus (L.) Rendle) is one of these herbs. The goal of this study was to see if an ethanol extract of lemongrass leaves could lower cholesterol levels in wistar rats. Maceration was used to extract the ethanol from lemongrass leaves. In pharmacological testing, 25 rats were separated into five groups: the control group, the comparison group for simvastatin 0.9 mg/kg bw, the test group at 13 mg/kg bw, 26 mg/kg bw, and 52 mg/kg bw, and the test group at 13 mg/kg bw, 26 mg/kg bw, and 52 mg/kg bw. Cholesterol 1g/kg bw and PTU 100 mg/kg bw were employed as inductors and were supplied for 14 days with the test preparation. The levels of cholesterol in the blood were tested. The results demonstrated that the ethanolic extract of lemongrass leaves could considerably reduce cholesterol levels (p<0.005) when compared to the control group. The ethanol extract dose of 52 mg/kg bw had the greatest inhibitory effect on cholesterol levels, implying that the ethanol extract of lemongrass leaves had anticholesterol potential.
Objective: Uses molecular docking and pharmacophore modeling methods to examine the antimalarial activity of apigenin (API) on distinct kinds and varieties of P. falciparum (Pf) receptors.
Methods: ...Using Autodock 4.0.1 and ligandscout software, molecular docking was conducted on multiple types of Pf receptors, including lactate dehydrogenase (Oxidoreductase), Enoyl-acyl carrier-protein (Oxidoreductase), Triose-phosphate (Isomerase), and plasmepsin II (Hydroxylase).
Results: The lowest free energy binding values found in two of the four investigations (API on an enoyl-acyl carrier and triose-phosphate receptors) suggested a potential effect. These values were-8.06 kcal/mol and-8.76 kcal/mol, respectively. The API had lower values of the inhibitory constant on the lactate dehydrogenase, enoyl-acyl carrier-protein, Triose-phosphate, and plasmepsin II receptors (44.06 µM, 1.24 µM, 376.76 nM, and 57.04 µM, respectively). In terms of the essential elements of amino acid residue interaction, the API and the native ligand were identical (SER218 for 1LF3 receptor; LEU315, GLY110, and TYR111 for 1NWH receptor; VAL212, LYS12, ASN233, and GLY232 for 1O5X receptor; and ILE31, PRO250, and PRO246 for 1U4O receptor). According to the findings of the pharmacophore modeling, the functional groups of hydroxyl were the most important functional groups to interact with the important amino acid residues of the receptors.
Conclusion: The API considerably displays competitive antimalarial potency in various Pf receptors.
Malaria dapat menyebabkan disfungsi multi organ yang dikaitkan oleh respon inflamasi yang dipicu salah satunya produksi mediator inflamasi yang dikeluarkan dari eritrosit terinfeksi yang lisis. ...Berdasarkan hasil penelitian sebelumnya resveratrol memiliki aktivitas farmakologi seperti imunomodulator, kardioprotektif, antiinflamasi, antioksidan, dan antimalaria. Tujuan dari penelitian ini mengevaluasi pengaruh resveratrol terhadap organ mencit yang terinfeksi malaria Plasmodium berghei ANKA. Mencit swiss webster yang telah terinfeksi P. berghei dikelompokan secara acak dan diberikan resveratrol 25, 50, 100 mg/kg bb secara per oral selama tujuh hari. Parameter pengamatan meliputi indeks organ dan histopatologi organ hati, limpa serta ginjal. Hasil menunjukan resveratrol pada dosis 25, 50, 100 mg/kg bb memiliki nilai indeks organ limpa, hati, dan ginjal yang lebih kecil bila dibandingkan dengan kelompok kontrol (P<0,05). Sedangkan hasil pengamatan histologi limpa, hati, dan ginjal pada mencit yang diberikan resveratrol berbagai dosis menunjukan adanya perbaikan sel. Resveratrol pada dosis 25, 50, 100 mg/kg bb mempengaruhi terhadap indeks organ mencit yang terinfeksi P. berghei dan merupakan salah satu indikator bahwa resveratrol dapat mengurangi komplikasi malaria.
