Biomass is currently seen as a promising renewable energy source, which can be sustainably utilized in the production of fuels and electric energy adding no carbon dioxide to the environment. ...Co-gasification has unveiled its potential amongst thermal techniques, as a result of the valuable products obtained, strengthening a solid position in the conversion of residues. Thus, the prevention of a complete depletion of non-renewable sources is supported and the effects of their utilization alleviated.
Extensive literature review was conducted and, few reports on co-gasification of biomass and wastes were found. In this context, this review addresses their thermal conversion, highlighting issues related to the equipment, operating conditions and physicochemical phenomena involved in such a complex process. Among other conclusions, the most important finding of this work was the synergy often encountered between the two feedstocks, proving co-gasification can overcome several of the individual gasification issues enhancing products quality and yields over biomass or wastes alone, and attesting its environmental-friendly character, with lower greenhouse gas emissions. It was also possible to depict some trends on the effect of biomass and waste blending ratios, as well as elucidating some of the mechanisms involved in their interaction. These are majorly explained by the response of molecules during pyrolysis and by hydrogen transfer from waste polymers to biomass derivatives. Experimental conditions were also assessed, fluidized beds being reported as the most suitable reactors for biomass and wastes, under several different possible combinations of operational parameters. A critical discussion is presented, aiming to contribute to a more profound understanding of this matter, its key points and noteworthy potential.
Summary Background Zika virus is an arthropod-borne virus that is a member of the family Flaviviridae transmitted mainly by mosquitoes of the genus Aedes . Although usually asymptomatic, infection ...can result in a mild and self-limiting illness characterised by fever, rash, arthralgia, and conjunctivitis. An increase in the number of children born with microcephaly was noted in 2015 in regions of Brazil with high transmission of Zika virus. More recently, evidence has been accumulating supporting a link between Zika virus and microcephaly. Here, we describe findings from three fatal cases and two spontaneous abortions associated with Zika virus infection. Methods In this case series, formalin-fixed paraffin-embedded tissue samples from five cases, including two newborn babies with microcephaly and severe arthrogryposis who died shortly after birth, one 2-month-old baby, and two placentas from spontaneous abortions, from Brazil were submitted to the Infectious Diseases Pathology Branch at the US Centers for Disease Control and Prevention (Atlanta, GA, USA) between December, 2015, and March, 2016. Specimens were assessed by histopathological examination, immunohistochemical assays using a mouse anti-Zika virus antibody, and RT-PCR assays targeting the NS5 and envelope genes. Amplicons of RT-PCR positive cases were sequenced for characterisation of strains. Findings Viral antigens were localised to glial cells and neurons and associated with microcalcifications in all three fatal cases with microcephaly. Antigens were also seen in chorionic villi of one of the first trimester placentas. Tissues from all five cases were positive for Zika virus RNA by RT-PCR, and sequence analyses showed highest identities with Zika virus strains isolated from Brazil during 2015. Interpretation These findings provide strong evidence of a link between Zika virus infection and different congenital central nervous system malformations, including microcephaly as well as arthrogryposis and spontaneous abortions. Funding None.
ObjectiveTo assess the prevalence of burn-out syndrome in healthcare workers working on the front line (FL) in Spain during COVID-19.DesignCross-sectional, online survey-based study.SettingsSampling ...was performed between 21st April and 3rd May 2020. The survey collected demographic data and questions regarding participants’ working position since pandemic outbreak.ParticipantsSpanish healthcare workers working on the FL or usual ward were eligible. A total of 674 healthcare professionals answered the survey.Main outcomes and measuresBurn-out syndrome was assessed by the Maslach Burnout Inventory-Medical Personnel.ResultsOf the 643 eligible responding participants, 408 (63.5%) were physicians, 172 (26.8%) were nurses and 63 (9.8%) other technical occupations. 377 (58.6%) worked on the FL. Most participants were women (472 (73.4%)), aged 31–40 years (163 (25.3%)) and worked in tertiary hospitals (>600 beds) (260 (40.4%)). Prevalence of burn-out syndrome was 43.4% (95% CI 39.5% to 47.2%), higher in COVID-19 FL workers (49.6%, p<0.001) than in non- COVID-19 FL workers (34.6%, p<0.001). Women felt more burn-out (60.8%, p=0.016), were more afraid of self-infection (61.9%, p=0.021) and of their performance and quality of care provided to the patients (75.8%, p=0.015) than men. More burn-out were those between 20 and 30 years old (65.2%, p=0.026) and those with more than 15 years of experience (53.7%, p=0.035).Multivariable logistic regression analysis revealed that, working on COVID-19 FL (OR 1.93; 95% CI 1.37 to 2.71, p<0.001), being a woman (OR 1.56; 95% CI 1.06 to 2.29, p=0.022), being under 30 years old (OR 1.75; 95% CI 1.06 to 2.89, p=0.028) and being a physician (OR 1.64; 95% CI 1.11 to 2.41, p=0.011) were associated with high risk of burn-out syndrome.ConclusionsThis survey study of healthcare professionals reported high rates of burn-out syndrome. Interventions to promote mental well-being in healthcare workers exposed to COVID-19 need to be immediately implemented.
Antibodies for therapeutic use are being continuously approved and their demand has been steadily growing. As known, the golden standard for monoclonal antibody (mAb) purification is Protein A ...affinity chromatography, a technology that has gained high interest because of its great performance and capabilities. The main concerns are the elevated resins costs and their limited lifetime compared to other resins (e.g. ion exchange chromatography). Great efforts have been carried out to improve purification conditions, such as resin characterization and designing alkali/acid stable resins with a longer lifetime. Modification of Protein A ligands and alternative formats such as monoliths membranes and microshperes have been tested to increase the purification performance. New technology has been proposed to improve the large‐scale separation; in addition, alternative ligands have been suggested to capture mAbs instead of Protein A ligand; however, most of the information is locked by pharmaceutical companies. This mini review summarizes and describes the advances, results, and impact on the Protein A chromatography purification processing.
Display omitted
Bone, tooth enamel, and dentin accumulate Sr2+, a natural trace element in the human body. Sr2+ comes from dietary and environmental sources and is thought to play a key role in ...osteoporosis treatments. However, the underlying impacts of Sr2+on bone mineralization remain unclear and the use of synthetic apatites (which are structurally different from bone mineral) and non-physiological conditions have led to contradictory results. Here, we report on the formation of a new Sr2+-rich and stable amorphous calcium phosphate phase, Sr(ACP). Relying on a bioinspired pathway, a series of Sr2+ substituted hydroxyapatite (HA) that combines the major bone mineral features is depicted as model to investigate how this phase forms and Sr2+ affects bone. In addition, by means of a comprehensive investigation the biomineralization pathway of Sr2+ bearing HA is described showing that not more than 10 at% of Sr2+, i.e. a physiological limit incorporated in bone, can be incorporated into HA without phase segregation. A combination of 31P and 1H solid state NMR, energy electron loss spectromicroscopy, transmission electron microscopy, electron diffraction, and Raman spectroscopy shows that Sr2+ introduces disorder in the HA culminating with the unexpected Sr(ACP), which co-exists with the HA under physiological conditions. These results suggest that heterogeneous Sr2+ distribution in bone is associated with regions of low structural organization. Going further, such observations give clues from the physicochemical standpoint to understand the defects in bone formation induced by high Sr2+ doses.
Understanding the role played by Sr2+ has a relevant impact in physiological biomineralization and provides insights for its use as osteoporosis treatments. Previous studies inspired by the bone remodelling pathway led to the formation of biomimetic HA in terms of composition, structures and properties in water. Herein, by investigating different atomic percentage of Sr2+ related to Ca2+ in the synthesis, we demonstrate that 10% of Sr2+ is the critical loads into the biomimetic HA phase; similarly to bone. Unexpectedly, using higher amount leads to the formation of a stable Sr2+-rich amorphous calcium phosphate phase that may high-dose related pathologies. Our results provide further understanding of the different ways Sr2+ impacts bone.
Energetic metabolism supports rapid cell growth and proliferation, differentiation, polarization, and effector functions of T cells. T lymphocytes have the remarkable plasticity that allows them to ...shape their metabolism to adapt to extracellular and intracellular cues, a process that involves molecular modules referred to as "metabolic checkpoints" that sense metabolic signals and transduce effector messages. These metabolic checkpoints may represent a novel therapeutic strategy for immune modulation. Chemical immunosuppressive drugs including mammalian target of rapamycin inhibitors (sirolimus and everolimus), calcineurin inhibitors (tacrolimus and cyclosporine), and purine and pyrimidine synthesis inhibitors (6-mercaptopurine, mycophenolic acid, and methotrexate) are widely prescribed for the treatment of autoimmune and inflammatory diseases and for controlling alloimmunity in interfering with the signals that activate and allow T cells to proliferate. Emerging evidence indicates that these drugs also target T-cell metabolism and metabolic checkpoints, which, as a consequence, could contribute to their immunosuppressive effects. These examples raise the issue of how the modulation of these metabolic checkpoints can regulate T-cell activation, differentiation, and function. In this review we highlight emerging concepts about the modulation of metabolic reprogramming in T-cell responses by immunosuppressive drugs and how potential therapeutic interventions influence T-cell fate and effector function.
Matrix metalloproteinases (MMPs) are important targets for pathological conditions such as arthritis, chronic obstructive pulmonary disease, and cancer. The failure of the first broad-spectrum MMP ...inhibitors in clinical trials has led researchers to address the selectivity as one of their main objectives. The S1' pocket has been widely used to modulate the selectivity of these enzymes because it displays the highest variability in length and shape among MMPs. In this review, we encourage medicinal chemists to also consider the dynamics of this pocket as an important parameter to achieve the desired selectivity. To support this proposal, we collect examples from the literature where the flexibility of the S1' pocket was highlighted as a relevant and significant issue affecting selectivity. We also review the experimental studies on the dynamics of this pocket.
In recent decades, scientific institutions have undergone significant changes due to new managerialism and the application of excellence in research. This research model has given rise to tensions ...related to increasing pressures and working demands in a competitive international environment that accelerate the pace of academic life. In addition, precarious working conditions and job insecurity have affected academics' lives and careers. Academic literature has already addressed these organizational changes and their impact on academics, however, few studies have focused on psychosocial risks related to time constraints, meritocratic pressures and career insecurity from a gender perspective. This analysis is relevant given the gendered distribution of responsibilities and the evidence of gender biases in academia that hinder the advancement of gender equality in scientific institutions, as the persistent lack of women at the top of research careers show. In this paper, we explore the psychosocial effects of the new organizational model of science characterized by accelerated time regimes and precarious working conditions from a gender perspective. We draw attention to gender-based discriminatory practices that may yield an accumulative effect on the well-being of women academics. We analyze 36 interviews from women and men researchers from five areas of knowledge in Spanish universities and research centers, following a 'gendered institutions' approach. The results highlight psychosocial risks for both men and women academics as a result of accelerated work organizations, intensified by uncertainty and hyper-competition due to lack of positions. The hegemonic male work model characterized by total availability confirms academia as a gendered institution, especially damaging women's well-being and careers, as well as those of men committed to care responsibilities - challenging motherhood explanations - which may discourage them from the pursuit of gender equality. Our findings highlight discriminatory practices toward women academics which create psychological harm and feelings of being unwelcome, putting their career progression at risk. Lastly, we suggest a different model of work organization following the implementation of a culture based on an 'ethics of care' feminist approach.
PDF
