How can moral transgressors rebuild their image as good people? Using affect control theory, I hypothesize that prosociality—benefitting others—will blunt negative impressions of a norm violator. I ...also hypothesize that benefitting good or weak people—and not bad or powerful people—will amplify the positive effect of prosociality. In two survey-vignette studies, participants reported their perceptions about a man who takes money from a found wallet—unethical behavior—and gives or does not give it to someone else—prosocial behavior. Results show prosociality redeems violators more when they help good rather than bad persons. In certain situations, helping powerless persons is more image revamping than helping powerful persons.
BACKGROUNDPassive immunotherapy with convalescent plasma (CP) is a potential treatment for COVID-19. Evidence from controlled clinical trials is inconclusive.METHODSWe conducted a randomized, ...open-label, controlled clinical trial at 27 hospitals in Spain. Patients had to be admitted for COVID-19 pneumonia within 7 days from symptom onset and not on mechanical ventilation or high-flow oxygen devices. Patients were randomized 1:1 to treatment with CP in addition to standard of care (SOC) or to the control arm receiving only SOC. The primary endpoint was the proportion of patients in categories 5 (noninvasive ventilation or high-flow oxygen), 6 (invasive mechanical ventilation or extracorporeal membrane oxygenation ECMO), or 7 (death) at 14 days. Primary analysis was performed in the intention-to-treat population.RESULTSBetween April 4, 2020, and February 5, 2021, 350 patients were randomly assigned to either CP (n = 179) or SOC (n = 171). At 14 days, proportion of patients in categories 5, 6, or 7 was 11.7% in the CP group versus 16.4% in the control group (P = 0.205). The difference was greater at 28 days, with 8.4% of patients in categories 5-7 in the CP group versus 17.0% in the control group (P = 0.021). The difference in overall survival did not reach statistical significance (HR 0.46, 95% CI 0.19-1.14, log-rank P = 0.087).CONCLUSIONCP showed a significant benefit in preventing progression to noninvasive ventilation or high-flow oxygen, invasive mechanical ventilation or ECMO, or death at 28 days. The effect on the predefined primary endpoint at 14 days and the effect on overall survival were not statistically significant.TRIAL REGISTRATIONClinicaltrials.gov, NCT04345523.FUNDINGGovernment of Spain, Instituto de Salud Carlos III.
The cryoglobulinaemias Ramos-Casals, Manuel, MD; Stone, John H, MD; Cid, Maria C, MD ...
The Lancet (British edition),
01/2012, Letnik:
379, Številka:
9813
Journal Article
Recenzirano
Cryoglobulins are immunoglobulins that precipitate in vitro at temperatures less than 37°C and produce organ damage through two main pathways: vascular sludging (hyperviscosity syndrome, mainly in ...type I cryoglobulinaemia) and immune-mediated mechanisms (principally vasculitis, in mixed cryoglobulinaemia). Cryoglobulinaemia is associated with many illnesses, which can be broadly grouped into infections, autoimmune disorders, and malignancies; the most common cause is infection with hepatitis C virus. Mixed cryoglobulinaemic syndrome is diagnosed when a patient has typical organ involvement (mainly skin, kidney, or peripheral nerve) and circulating cryoglobulins. Cutaneous purpura is the most common manifestation of cryoglobulinaemic vasculitis. The most frequently affected internal organs are the peripheral nerves, kidneys, and joints. The course varies widely and prognosis is influenced by both cryoglobulinaemic damage to vital organs and by comorbidities associated with underlying diseases. More than 90% of cases of cryoglobulinaemia have a known underlying cause; therefore treatment is focused on the cause of the disorder rather than merely symptomatic relief. Studies suggest that both combined or sequential antiviral therapies and targeted biological treatments might be more effective than monotherapy.
ZnO thin films having a nanocolumnar microstructure are grown by plasma‐enhanced chemical vapor deposition at 423 K on pre‐treated fluorine‐doped tin oxide (FTO) substrates. The films consist of ...c‐axis‐oriented wurtzite ZnO nanocolumns with well‐defined microstructure and crystallinity. By sensitizing CH3NH3PbI3 on these photoanodes a power conversion of 4.8 % is obtained for solid‐state solar cells. Poly(triarylamine) is found to be less effective when used as the hole‐transport material, compared to 2,2′,7,7′‐tetrakis(N,N‐di‐p‐methoxyphenylamine)‐9,9′‐spirobifluorene (spiro‐OMeTAD), while the higher annealing temperature of the perovskite leads to a better infiltration in the nanocolumnar structure and an enhancement of the cell efficiency.
ZnO can work as an electron conduit in the fabrication of perovskite‐based solid‐state solar cells. Nanocolumnar ZnO thin films synthesized by plasma‐enhanced chemical vapor deposition are effective candidates for large‐area deposition.
•Thymol and D43B were added to PLA to get antioxidant nano-biocomposite films.•A full characterization of all formulations was carried out.•The presence of thymol in the films and its antioxidant ...activity were demonstrated.•The addition of D43B improved oxygen barrier and mechanical properties of films.•The film with thymol and 2.5wt% of D43B was considered the best enhanced material.
Novel nano-biocomposite films based on poly (lactic acid) (PLA) were prepared by incorporating thymol, as the active additive, and modified montmorillonite (D43B) at two different concentrations. A complete thermal, structural, mechanical and functional characterization of all nano-biocomposites was carried out. Thermal stability was not significantly affected by the addition of thymol, but the incorporation of D43B improved mechanical properties and reduced the oxygen transmission rate by the formation of intercalated structures, as suggested by wide angle X-ray scattering patterns and transmission electron microscopy images. The addition of thymol decreased the PLA glass transition temperature, as the result of the polymer plasticization, and led to modification of the elastic modulus and elongation at break. Finally, the amount of thymol remaining in these formulations was determined by liquid chromatography (HPLC-UV) and the antioxidant activity by the DPPH spectroscopic method, suggesting that the formulated nano-biocomposites could be considered a promising antioxidant active packaging material.
In its classical form, autophagy is an essential, homeostatic process by which cytoplasmic components are degraded in a double-membrane-bound autophagosome in response to starvation. Paradoxically, ...although autophagy is primarily a protective process for the cell, it can also play a role in cell death. The roles of autophagy bridge both the innate and adaptive immune systems and autophagic dysfunction is associated with inflammation, infection, neurodegeneration and cancer. In this review, we discuss the contribution of autophagy to inflammatory, infectious and neurodegenerative diseases, as well as cancer.
•Autophagy is a homeostatic process by which cell components are degraded.•Autophagy can also play a role in cell death.•Autophagy contributes to host defense and inflammation.•Autophagic dysfunction is associated with cancer.
Knowledge of the soil water balance is fundamental for improving crop water use in agricultural fields. Estimates are normally for representative and homogeneous areas where the variability of soil ...properties is neglected. However, this variability significantly impacts soil water dynamics at the field scale. In this study, the MOHID-Land distributed process-based model was used to compute the spatially explicit soil water dynamics in a 22.6-ha almond field located in southern Portugal. An electromagnetic induction survey was first performed to obtain electromagnetic images of the real soil conductivity in depth, which were related to soil texture. Then, pedotransfer functions were used to convert soil texture into soil hydraulic data. MOHID-Land results included maps of the spatial distribution of soil water contents, actual crop transpiration, actual soil evaporation, percolation below the rootzone, and surface runoff. These allowed identifying preferential flow pathways as well as the main control factors influencing soil water dynamics at the field scale. Some development needs were identified, and overcoming them would enhance the significance of contributions such as this study to the field of precision agriculture.
•The spatial variability of the soil water balance was assessed in an almond field.•Soil properties were inferred from an electromagnetic induction (EMI) survey.•Distributed modeling identified crop growth areas and preferred flow paths at the field scale.•Limitations of the modeling approach were identified and discussed.•The development of approaches combining distributed modeling and EMI sensing is of interest to precision agriculture.
Melanopsin has been implicated in the mammalian photoentrainment by blue light. This photopigment, which maximally absorbs light at wavelengths between 470 and 480 nm depending on the species, is ...found in the retina of all classes of vertebrates so far studied. In mammals, melanopsin activation triggers a signaling pathway which resets the circadian clock in the suprachiasmatic nucleus (SCN). Unlike mammals, Drosophila melanogaster and Danio rerio do not rely only on their eyes to perceive light, in fact their whole body may be capable of detecting light and entraining their circadian clock. Melanopsin, teleost multiple tissue (tmt) opsin and others such as neuropsin and va-opsin, are found in the peripheral tissues of Danio rerio, however, there are limited data concerning the photopigment/s or the signaling pathway/s directly involved in light detection. Here, we demonstrate that melanopsin is a strong candidate to mediate synchronization of zebrafish cells. The deduced amino acid sequence of melanopsin, although being a vertebrate opsin, is more similar to invertebrate than vertebrate photopigments, and melanopsin photostimulation triggers the phosphoinositide pathway through activation of a G(q/11)-type G protein. We stimulated cultured ZEM-2S cells with blue light at wavelengths consistent with melanopsin maximal absorption, and evaluated the time course expression of per1b, cry1b, per2 and cry1a. Using quantitative PCR, we showed that blue light is capable of slightly modulating per1b and cry1b genes, and drastically increasing per2 and cry1a expression. Pharmacological assays indicated that per2 and cry1a responses to blue light are evoked through the activation of the phosphoinositide pathway, which crosstalks with nitric oxide (NO) and mitogen activated protein MAP kinase (MAPK) to activate the clock genes. Our results suggest that melanopsin may be important in mediating the photoresponse in Danio rerio ZEM-2S cells, and provide new insights about the modulation of clock genes in peripheral clocks.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
► Thymol and carvacrol were incorporated to PP to get antimicrobial active films. ► A full characterization of all formulations was carried out. ► The presence of additives in the polymer matrix ...after processing was demonstrated. ► The antimicrobial activity of films against two bacteria was evaluated. ► PP films have demonstrated their ability as supporting matrix for active additives.
Antimicrobial active films based on polypropylene (PP) were prepared by incorporating thymol and carvacrol at three different concentrations: 4, 6 and 8wt.% of both additives as well as an equimolar mixture of them. A complete thermal, structural, mechanical and functional characterization of all formulations was carried out. SEM micrographs showed certain porosity for films with high additives concentrations. A decrease in elastic modulus was obtained for the active formulations compared with neat PP. The presence of additives did not affect the thermal stability of PP samples, but decreased PP crystallinity and oxygen barrier properties. The presence of thymol and carvacrol also increased stabilization against thermo-oxidative degradation, with higher oxidation induction parameters. Finally, thymol showed higher inhibition against bacterial strain present in food compared with carvacrol, leading to higher antimicrobial activity. The obtained results proved the permanence of certain amounts of the studied additives in the polymer matrix after processing making them able to be used as active additives in PP formulations.
Ceruloplasmin, the main copper binding protein in blood plasma, has been of particular interest for its role in efflux of iron from cells, but has additional functions. Here we tested the hypothesis ...that it releases its copper for cell uptake by interacting with a cell surface reductase and transporters, producing apoceruloplasmin. Uptake and transepithelial transport of copper from ceruloplasmin was demonstrated with mammary epithelial cell monolayers (PMC42) with tight junctions grown in bicameral chambers, and purified human (64)Cu-labeled ceruloplasmin secreted by HepG2 cells. Monolayers took up virtually all the (64)Cu over 16h and secreted half into the apical (milk) fluid. This was partly inhibited by Ag(I). The (64)Cu in ceruloplasmin purified from plasma of (64)Cu-injected mice accumulated linearly in mouse embryonic fibroblasts (MEFs) over 3-6h. Rates were somewhat higher in Ctr1+/+ versus Ctr1-/- cells, and 3-fold lower at 2 °C. The ceruloplasmin-derived (64)Cu could not be removed by extensive washing or trypsin treatment, and most was recovered in the cytosol. Actual cell copper (determined by furnace atomic absorption) increased markedly upon 24h exposure to holoceruloplasmin. This was accompanied by a conversion of holo to apoceruloplasmin in the culture medium and did not occur during incubation in the absence of cells. Four different endocytosis inhibitors failed to prevent 64Cu uptake from ceruloplasmin. High concentrations of non-radioactive Cu(II)- or Fe(III)-NTA (substrates for cell surface reductases), or Cu(I)-NTA (to compete for transporter uptake) almost eliminated uptake of (64)Cu from ceruloplasmin. MEFs had cell surface reductase activity and expressed Steap 2 (but not Steaps 3 and 4 or dCytB). However, six-day siRNA treatment was insufficient to reduce activity or uptake. We conclude that ceruloplasmin is a circulating copper transport protein that may interact with Steap2 on the cell surface, forming apoceruloplasmin, and Cu(I) that enters cells through CTR1 and an unknown copper uptake transporter.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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